Labshake search
Citations for Zymo Research :
101 - 150 of 375 citations for Peptide Standard 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The plasmids with the A/B standards were then extracted and purified using the ZymoPURE Plasmid Miniprep Kit (Zymo Research), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: The Zymobiomics microbial community DNA standard consisting of 8 bacterial and 2 fungal genomic DNAs (cat. no. D6305) was obtained from Zymo Research (Irvine ...
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bioRxiv - Microbiology 2021Quote: ... used in this study has a log abundance distribution of different bacterial species and fungi ranging from 108 to 102 cells (ZymoBIOMICS Microbial Community Standard II, ZYMO RESEARCH EUROPE GMBH ...
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bioRxiv - Microbiology 2020Quote: ... see below) to which we added 50 μl of ZymoBIOMICS Microbial Community Standard Cells (Zymo Research Corp., Irvine, CA, USA). Mock sap samples were then processed as indicated above for the PowerPlant DNA extraction protocol ...
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bioRxiv - Genomics 2023Quote: ... High molecular weight DNA extracted from whole blood by standard protocols was bisulfite converted with the EZ-96 DNA Methylation-Gold Kit (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... from weight-normalized contents from the distal small intestine and proximal colon after addition of Low Abundance Microbiota Standard (Zymo) and extraction using the Zymo DNA Mini kit (Zymo) ...
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bioRxiv - Molecular Biology 2023Quote: All RNA reporters were made by in vitro transcription with a standard T7 RNA polymerase protocol using DNA template gel extracted using the Zymoclean Gel DNA Recovery Kit (Zymo), 1x T7 RNA Polymerase buffer (NEB) ...
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bioRxiv - Genetics 2021Quote: ... We extracted and isolated RNA by phase separation using standard protocols followed by purification using the Direct-zol RNA microprep kit (Zymo R2060) with an on-column DNAse step per manufacturer directions ...
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bioRxiv - Genomics 2021Quote: ... The yeast DNA samples were collected by thawing frozen yeast and following the standard procedure of a Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research).
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bioRxiv - Molecular Biology 2022Quote: ... All the steps from here were optimized from the standard protocol provided with the EZ Nucleosomal DNA Prep Kit (D5220, Zymo Research). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction reagent only controls were included for each batch of DNA extractions along with ZymoBIOMICS Microbial Community Standards (Zymo Research) and E ...
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bioRxiv - Molecular Biology 2020Quote: ... The linear A/B standards were finally treated with Proteinase K and further purified with the Zymo ChIP DCC-25 purification kit (Zymo Research). The final A/B standards were quantified using Qubit dsDNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial DNA was extracted from the fecal and intestinal samples and from two negative extraction controls and two ZymoBIOMICS Microbial Community Standard positive extraction controls (D6300 Zymo Research) with the DNeasy PowerSoil Pro Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: A standard whole-cell mixture consisting of 21 representative strains from 17 species of the gut microbiota (Zymo Research, ref D6331) was divided into 10 aliquots and stored in the storage solution provided by the manufacturer at −80 °C ...
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bioRxiv - Plant Biology 2020Quote: ... As a positive control we included a mock microbial community in three wells (ZymoBiomics Microbial Community Standard, Zymo Research, Irvine, CA, USA). We used the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... we added negative DNA extractions and PCR controls from each time point and mock communities (ZymoBIOMICS microbial community standard, Zymo, Irvine, CA) to each library for additional quality control and inference.
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bioRxiv - Microbiology 2019Quote: ... and a mock microbial community DNA (8 bacterial + 2 yeasts species mixed in defined proportions) (ZymoBIOMICS Microbial Community DNA standard, Zymo Research, USA) were included in the experiments ...
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bioRxiv - Plant Biology 2019Quote: ... to several others we added a mock microbial community as a positive control (ZymoBiomics Microbial Community Standard, Zymo Research, Irvine, CA, USA). We extracted DNA using the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics ...
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bioRxiv - Immunology 2022Quote: ... Samples were either kept in −80C at this point or proceeded with standard Trizol-mediated RNA extraction protocol or the Direct-zol 96 kit (Zymo Research, R2054) was used following the on-column DNA digestion protocol ...
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bioRxiv - Genomics 2023Quote: Reads were aligned to the 16S genes extracted from the complete reference genomes of the bacteria present in the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research D6305) using minimap2 ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 10 trunk fragments per biological replicate using the standard Trizol RNA extraction protocol with high salt precipitation and clean-up on Zymo RNA columns (Zymo R1013/R1017). RNA (1 μg ...
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bioRxiv - Microbiology 2021Quote: ... buffer without any added fecal material) and two microbial mock communities as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, California, USA).
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bioRxiv - Microbiology 2022Quote: ... Reagent blanks were included as negative controls and cells from a microbial community of known composition were included as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, CA). The V4 region of the 16S rRNA gene was amplified from approximately 5 ng of extracted DNA in 25μl reactions using Q5 HS High-Fidelity polymerase (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: ... we also produced ten separate V3-V4 16S rRNA gene amplicon libraries from the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA) using the 2-Step library preparation method ...
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bioRxiv - Neuroscience 2024Quote: ... A negative control with the control from DNA extraction and a positive control of ZymoBIOMICS™ Microbial Community Standard (ZYMO Research, Irvine, CA) were set during amplicon library preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer specificity was tested by performing ddPCR in the presence of an excess of eukaryotic (fish) or prokaryotic [ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA, USA)] DNA ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was also performed on three no-sample added (negative) and three ZymoBIOMICS Microbial Community Standard (positive) controls (Zymo Research, Irvine, CA, USA). DNA from an additional eight GWSS had been previously extracted using the Blood and Tissue kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... we then tested the fidelity of this kit by extracting DNA from gypsum spiked with cells from a mock community (ZymoBIOMICS Microbial Community Standard, Zymo Research, Irvine, CA, USA). We added 25 μL of this standard (∼4.7×108 cells ...
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bioRxiv - Microbiology 2022Quote: ... a negative control to account for microbial contaminants present in DNA extraction kits or introduced during the extraction (Eisenhofer et al., 2019) and a microbial community standard (Zymo Research, cat. no. D6300, Irving, CA). The DNA concentration was quantified with Qubit 3 using the high sensitivity dsDNA kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... we generated a mock microbiota community suspension using two commercially available reference standards: the first was the ZymoBIOMICS™ faecal reference kit with TruMatrix™ technology (D6323; Zymo Research, Irvine CA, USA); DNA content ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 ml Trizol (R2050-1, Zymo Research) as well as 200 µl chloroform were added to the interface and phase separation and removal were repeated ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were suspended in 6 ml of 1:1 mix of 1 M sorbitol: YPDplus medium (Zymo Research) and incubated at 30°C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... filled with 1% FBS-HBSS or Tri Reagent (Zymo, #R2050-1-200). Tissue was homogenized in an Omni Bead Ruptor 24 (5.15 ms ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were directly lysed in 1 ml of Trizol (R2050-1, Zymo Research) and subjected to interface cleanup as described below.
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bioRxiv - Molecular Biology 2023Quote: ... Round 1 PCRs were cleaned using DNA Binding Buffer (Zymo ZD4004-1-L) and UPrep Micro Spin Columns (Genesee Scientific 88–343) ...
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bioRxiv - Neuroscience 2021Quote: ... or TRI Reagent (Zymo, #R2050-1) for gene expression analysis ...
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bioRxiv - Genomics 2021Quote: ... digestion with 1% zymolyase (Zymo Research) to weaken ascus walls ...
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bioRxiv - Microbiology 2020Quote: An aliquot of each fraction was then mixed 1:1 with 2× DNA/RNA Shield (Zymo Research) spiked with in vitro transcribed RNA (generated by T7 transcription off a Firefly luciferase (Fluc)-containing plasmid) ...
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bioRxiv - Cancer Biology 2020Quote: Approximately 1.4kb of genomic fragment spanning SALL4 5’ UTR - exon 1 - intron 1 was PCR amplified (Zymo Research) and cloned into the pGEM-T Easy vector ...
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bioRxiv - Molecular Biology 2023Quote: ... Infectious viral stocks were inactivated by a 1:3 dilution with TRI Reagent® (Zymo, R2050-1-200) immediately prior to RNA extraction ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from mixtures of KU-1 or BL3.1 and BTL26 for a total of 1 × 106 cells using Quick-DNA Miniprep Kits (Zymo Research). The DNA concentrations of the samples were measured by UV absorbance at 260 nm using a NanoDrop 8000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... all mouse tissues obtained from BSL3 were treated with 1% Triton by volume or placed in TRIzol (Zymo R2050-1) to inactivate virus.
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bioRxiv - Bioengineering 2022Quote: ... and 1 mg/ml Proteinase K (Zymo Research) for 1 hour at 55°C ...
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bioRxiv - Microbiology 2021Quote: ... A 10:1 RNA/DNA shield from Zymo was added to each saliva sample to maintain samples during shipping.
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bioRxiv - Microbiology 2022Quote: ... and 100μL TRIzol (Zymo Research, R2050-1-50) was added to each well followed by isolation of total RNA using Direct-Zol RNA Mini-Prep Plus Kit (Zymo Research ...
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bioRxiv - Immunology 2020Quote: PCR testing for SARS-CoV-2 was carried out on respiratory specimens mixed 1:1 in DNA/RNA Shield (Zymo Inc) using an in-house Clinical Laboratory Improvement Amendments (CLIA ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with RNase for 1 hour and proteinase K for 1 hour before ChIP DNA Clean & Concentrator (Zymo Research) to extract the DNA.
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bioRxiv - Developmental Biology 2022Quote: ... 1 x ZymoTaq PreMix (Zymo Research, Irvine CA, USA) and 10μM of each primer ...
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bioRxiv - Genomics 2020Quote: ... with Oligo binding buffer (Zymo Research, D4060-1-10). The final product was assumed to have full yield (for a list of sequences ...
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bioRxiv - Cancer Biology 2022Quote: ... then homogenized with TRI Reagent (Zymo Research, R2050-1) by the gentleMACS Dissociator (Miltenyi Biotec 130-093-235) ...