Labshake search
Citations for Zymo Research :
1 - 50 of 60 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... at 30°C for 30 min and removed by RNA Clean & Concentrator (Zymo Research). Reverse transcription reactions including the ligated RNA above ...
-
bioRxiv - Genomics 2022Quote: ... into the 20 μl ligation reaction for incubation with 30 minutes at 30°C and 3 minutes at 65°C to inactivate the enzymes followed by column purification (Zymo Research, R1016). Before the reverse transcription (RT) ...
-
bioRxiv - Biochemistry 2023Quote: ... cleaned and eluted in 30 μL water (Zymo Clean and Concentrate).
-
bioRxiv - Biochemistry 2023Quote: ... 30 μL of the reaction mixture was collected and purified by ZYMO Oligo Clean & Concentrator spin column (ZYMO Research ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 30min at 30°C followed by column purification (Zymo research, R1016). The following reverse transcription (RT ...
-
bioRxiv - Cancer Biology 2024Quote: ... 30 µL of homogenously suspended mag-beads (Zymo research PN: R2130/R2131), and 400 µL of 100% ethanol (Acros organics ...
-
bioRxiv - Cell Biology 2024Quote: ... 30 minutes and purified by RNA Clean&Concentrator-5 (Zymo Research, R1014). cDNA libraries (100 ng RNA ...
-
bioRxiv - Cancer Biology 2020Quote: ... Total RNA was eluted in RNAse/DNAse-free water (Zymo Research, W1001-30) and submitted to the University of Chicago Genomics Facility for further analysis.
-
bioRxiv - Molecular Biology 2021Quote: ... #C3040) at 30°C and purified using the Zyppy Plasmid Miniprep kit (Zymo Research, #D4036) or ZymoPURE II Plasmid Midiprep kit (Zymo Research ...
-
bioRxiv - Molecular Biology 2020Quote: ... at 37 °C for 30 minutes and was cleaned using RNA Clean & Concentrator (Zymo Research).
-
bioRxiv - Molecular Biology 2021Quote: ... 30 or 60 s by adding binding buffer from the DNA Clean & Concentrator-25 kit (Zymo Research) supplemented with 30 mM EDTA and 37 mM sodium acetate ...
-
bioRxiv - Biochemistry 2020Quote: ... DNA was recovered from 30 individual clones using a Zymoprep yeast plasmid miniprep II kit (Zymo Research) and sequenced ...
-
bioRxiv - Microbiology 2023Quote: ... at 37°C for 30 min followed by cleanup using RNA concentrate and clean kit (ZYMO lnc.). For Northern blot analysis of HCV genomic RNA and vcircRNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 30 min and then total RNA collected using Direct-Zol RNA Miniprep Plus Kit (Zymo, R2070). Bru-labeled nascent RNA was then enriched 47 using anti-BrdU antibodies conjugated to magnetic beads ...
-
bioRxiv - Developmental Biology 2024Quote: ... at 37°C for 30 minutes and treated with the RNA Clean and Concentrator-5 kit (Zymo Research). The purity and concentration of the resulting RNA were assessed using Nanodrop (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2024Quote: ... at 37°C for 30 minutes and treated with the RNA Clean and Concentrator-5 kit (Zymo Research). A total of 1 µg of RNA was used as a template for cDNA synthesis using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 30 min at 37 °C and column-purified using the RNA Clean & Concentrator-5 kit (Zymo Research), eluting in 12.5 μL RNase-free water ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... for 30 minutes at 37 °C and products were further cleaned with RNA clean & concentrator 5 (Zymo Research) and eluted with 8-10 µl RNase free water ...
-
bioRxiv - Genomics 2022Quote: ... The reactions were incubated at 37ºC for 30 minutes and then cleaned up with a Clean and Concentrate-25 column (Zymo). To elute pools ...
-
bioRxiv - Cancer Biology 2023Quote: ... The samples were incubated (37°C, 30 min) and purified with the Zymo RNA Clean & Concentrator Kit (Zymo Research). The concentration of purified RNA was determined (Qubit™ RNA HS Assay Kit) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... and RNA extracted using Direct-zol columns with a 30 minute on-column DNase step at room temperature (Zymo Research). cDNA was generated with Superscript III First-Strand Synthesis SuperMix (Life Technologies) ...
-
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
-
bioRxiv - Genomics 2024Quote: ... Eluted DNA was treated with Proteinase K at 55ºC for 30 min and then purified with a ChIP DNA Clean and Concentrator kit (Zymo Research) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
-
bioRxiv - Microbiology 2022Quote: ... total RNA was eluted from the column in 60 uL of Buffer AVE (QIAmp) or from the beads in 30 uL of DNase/RNase-free water (Zymo) and immediately used for RT-qPCR and library preparation ...
-
bioRxiv - Genomics 2024Quote: ... in 100 µL volume for 30 min at 37 °C and then further purified by spin column (Zymo Research, R1013) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... The reactions were incubated for 30 min at 37°C and immediately purified using ZYMO RNA Clean & Concentrator (ZYMO RESEARCH, D7011). The purified RNA samples were eluted in 20 μl of nuclease free water ...
-
bioRxiv - Biochemistry 2022Quote: ... These sorted libraries were grown for 1 day at 30°C shaking in SD-CAA media and then 300 µl of the cultures were miniprepped (Zymo Research) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: ... Transposition was carried out at 37°C for 30 minutes followed by DNA purification with DNA Clean and Concentrator-25 (Zymo Research) according to manufacturer’s recommendation ...
-
bioRxiv - Cell Biology 2021Quote: ... Mixture was incubated at 37 ℃ for 30 min followed by purification with a Zymo DNA Clean and Concentrator-5 Kit (Zymo, DP4033). Transposed DNA was eluted in 24 μl elution buffer and amplified by PCR reaction (10 μl 5 X TAB ...
-
bioRxiv - Genomics 2021Quote: ... The mixture was incubated at 37°C for 30 minutes and subsequently purified using the ChIP DNA purification kit (Zymo Research) as described by the manufacturer ...
-
bioRxiv - Microbiology 2022Quote: ... were incubated in a total volume of 20 µL for 30 min at 37°C and subsequently column-purified using RNA Clean and concentrator 5 (Zymo Research).
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were either resuspended in 30 µL 100% RNALater and stored at -70°C or resuspended in 100 µL TRI reagent (Zymo Research). Samples were crushed with a micropestle and RNA was extracted using a Direct-zol RNA MicroPrep kit (Zymo Research).
-
bioRxiv - Genetics 2023Quote: ... Equal volumes (at least 30 µL) of each culture were pooled and DNA was extracted using the YeaStarTM genomic DNA kit (ZYMO research).
-
bioRxiv - Cell Biology 2023Quote: ... The RNAse treatments were carried out for 20 min at 30°C followed by RNA extraction using DirectZolTM (Zymo Research®) kits as per manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from the heads of 25-30 adult male flies using TRIzol Reagent and Direct-zol RNA microprep kit (Zymo Research) followed by cDNA preparation from 0.5–1 μg total RNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
-
bioRxiv - Molecular Biology 2024Quote: ... treated with 1.25 units of RQ1 DNase for 30 minutes at 37°C and RNA recovered using an RNA Clean & Concentrator-25 spin column kit (Zymo Research), following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... All 30 samples were pooled in an equivolume and purified with RNA Clean & Concentrator-5 columns (Zymo Research, Irvine, CA, USA). RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 30 min at 37°C and further purified by using a Zymo DNA Clean and Concentrator column (Zymo Research, D4033).
-
bioRxiv - Microbiology 2024Quote: ... using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research; Tustin, CA). Eluted RNA was analyzed in a Nanodrop Lite (Thermo Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... Prototrophic colonies were grown in uracil dropout medium (MilliporeSigma) at 30 °C and 250 rpm and the plasmid was purified using the Yeast Plasmid MiniPrep II Kit (Zymo Research). The plasmid was transformed into TransforMax EPI300TM E ...
-
bioRxiv - Molecular Biology 2022Quote: ... a total of 30 μg of isolated total RNA per replicate was purified with the Quick-RNA Microprep Kit (Zymo research) and 1 μg of purified RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad) ...
-
bioRxiv - Evolutionary Biology 2022Quote: RNA was extracted from 30 μL of each virus inoculum using a Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... The resulting RNA pellets were resuspended in water and digested with 2 U of DNAse I for 30 min at 37 °C followed by purification using RNA clean & concentrator 5 columns (Zymo Research). mRNA levels in every fraction was quantified by RT-qPCR as described above ...
-
bioRxiv - Molecular Biology 2024Quote: ... The resulting RNA was digested with 2 U of DNAse I for 30 min at 37 °C and further purified using RNA clean & concentrator 5 columns (Zymo research). About 1.5 μg of total RNA was retrotranscribed with hexamers using Superscript VI retrotranscriptase (Invitrogen) ...
-
bioRxiv - Genetics 2024Quote: ... recovered for 1 hour at 30°C with shaking and then grown for 12-16 hours at 30°C prior to plasmid preparation using ZymoPURE II kits (Zymo Research). To generate the final homology-directed repair template (HDRT ...
-
bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
-
bioRxiv - Molecular Biology 2022Quote: ... The linear plasmid was excised from the gel and purified to 30 μl mQ using the Zymoclean™ Gel DNA Recovery Kit by Zymo (D4002). The codon random mRFP library (15 μl ...
-
bioRxiv - Genetics 2023Quote: RNA was extracted from each of the 384 samples in batches of 30 samples using the Direct-zol RNA Miniprep kit (Zymo Research, R2050). Batches consisted of samples from the same tissue and were processed in the order individual samples were collected ...