Labshake search
Citations for Zymo Research :
401 - 450 of 501 citations for Mouse PNP shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: DNA of the active population was extracted from yeast cells using Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) and amplified in two steps ...
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bioRxiv - Bioengineering 2023Quote: Plasmids of enriched nanobodies were isolated from the terminal round of sorting using a yeast miniprep kit (Zymo, D2004). For nanobody Fc fusions ...
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bioRxiv - Synthetic Biology 2023Quote: ... and sequence confirmed (Sequetech) and then maxi-prepped per the manufacturer’s protocols (ZymoPure II Plasmid Maxiprep Kit, Zymo Research). Plasmids were sterile filtered using a 0.22-μm syringe filter and stored at 4 °C.
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bioRxiv - Microbiology 2024Quote: ... Infantis plasmid status was monitored by DNA extraction using ZR BAC DNA Miniprep Kit (Zymo Research; Irvine, CA, US) followed by agarose gel electrophoresis of plasmid linearizd with NotI ...
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bioRxiv - Biochemistry 2024Quote: ... DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004). VH sequences were amplified by PCR and submitted to Genewiz for Amplicon NGS sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... Colonies were collected with 1.5mL sterile DDW and extracted using Zymoprep Yeast Plasmid Miniprep II (Zymo Research, CAT #D2004).
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bioRxiv - Cell Biology 2020Quote: ... and primary mouse keratinocytes was isolated using Direct-zol RNA Microprep Kits (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: RNA from mouse lungs was extracted using RNA Miniprep Plus Kit (Zymo Research). Briefly ...
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bioRxiv - Synthetic Biology 2019Quote: ... Then 1 mL of the culture was removed for DNA extraction using the Zymoprep yeast Plasmid Miniprep II (Zymo Research) kit ...
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bioRxiv - Biochemistry 2021Quote: ... Quick-RNA miniprep Kit (Cat. No. R1054) and ZymoPURE II Plasmid Maxiprep Kit (Cat. No. D4203) were obtained from Zymo Research ...
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bioRxiv - Biochemistry 2021Quote: ... Isolated colonies were expanded into overnight cultures and the pDNA was isolated with the ZymoPURE Plasmid MiniPrep according to manufacturer’s protocol (Zymo Research). The mutation in the sequence was confirmed by sequencing (Macrogen).
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bioRxiv - Pathology 2021Quote: ... cerevisiae strains PLY240 (MATa his3Δ200 leu2-3,112 trp1Δ901 ura3-52 suc2Δ9 trk1Δ51 trk2Δ50::lox-kanMX-lox) [55] was transformed with the constructed pYES2sh plasmids using Frozen-EZ Yeast Transformation II kit (Zymo Research Europe GmbH ...
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bioRxiv - Genetics 2022Quote: Yeast colonies containing bait and prey constructs that grew on Trp−/Leu−/His− + 25 mM 3-Amino-1,2,4-triazole (3AT) selection plates were extracted using the Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004) followed by Sanger sequencing for gene identification ...
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bioRxiv - Plant Biology 2019Quote: ... The plasmid was linearized using the restriction enzyme SacI and purified using the DNA Clean and Concentrator Kit (Zymo Research). We used the TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... frozen yeast were thawed and then their plasmids were extracted using a DNA Clean and Concentrator kit (Zymo Research, D4013). Extracted plasmids were prepped and sequenced using Sanger sequencing ...
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bioRxiv - Genomics 2019Quote: ... Products were run on a 0.8% TAE agarose gel and linearized plasmids were isolated with Zymoclean Gel DNA Recovery Kit (Zymo Research). Vectors were then digested in a similar fashion with XbaI (New England Biolabs ...
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bioRxiv - Synthetic Biology 2020Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2019Quote: DNA was extracted from each of the 96 isolates using a ZR Plasmid Miniprep – Classic Kit (Zymo Research, Irvine, CA). After quality control analysis on an Agilent TapeStation ...
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bioRxiv - Microbiology 2021Quote: ... all PCR reactions were DpnI treated to digest the methylated plasmid template DNA before the PCR fragments were purified with the DNA Clean & Concentrator Kit (Zymo). To construct pMRE-Himar-13X ...
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bioRxiv - Genetics 2020Quote: ... DNA extractions by miniprep were done with the ZymoPURE Plasmid Miniprep kit and elution with water (Zymo Research, cat. D4208T).
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bioRxiv - Bioengineering 2020Quote: ... Around 50 ml of yeast culture was harvested and DNA was extracted using Zymoprep Yeast Plasmid Miniprep II (Zymo Research). Two rounds of PCR were performed to amplify the coding sequence of the selected proteins and attach standard Illumina sequencing adaptors ...
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bioRxiv - Molecular Biology 2022Quote: ... Linearized plasmid DNAs and RNA bait DNAs were kit purified prior to IVTT (DNA Clean and Concentrator; Zymo Research, D4004). All DNAs were eluted into Tris-EDTA (TE ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid-backbone and insert fragments were isolated from gels with the Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine CA). DNA concentrations were measured with a NanoDrop 2000 spectrophotometer (Thermo Fisher ...
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bioRxiv - Genetics 2023Quote: ... coli-derived Ydj1-CXXX plasmid library was transformed into yWS304 (ydj1Δ) via Frozen-EZ Yeast transformation II Kit by Zymo Research (Irvine ...
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bioRxiv - Biochemistry 2023Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells were prepared and transformed with plasmids using the Mix and Go™ kit (Zymo Research, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Synthetic Biology 2023Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Bioengineering 2023Quote: The terminal sorts of the yeast-displayed libraries were miniprepped using the Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research, D2004) to recover enriched yeast plasmids ...
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bioRxiv - Genetics 2024Quote: ... A cell line was prepared encoding this donor plasmid by transforming BL21(DE3) and making the resultant cell line chemically competent using Mix & Go preparation kit (Zymo). The temperature sensitive donor plasmid was then transformed with a second plasmid encoding a T7-inducible recombinase and a constitutively expressed bridge RNA ...
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bioRxiv - Cell Biology 2023Quote: gDNA was isolated from mouse gastrocnemius using Quick-DNA Microprep Plus Kit (Zymo D4074). gDNA was amplified using CloneAmp™ HiFi PCR Premix (Takara 639298 ...
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Synthetic Biology 2020Quote: ... yeast strain was used to make competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research).
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bioRxiv - Bioengineering 2019Quote: ... 1.5-mL aliquots of cell culture were used as input in minipreps with the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Isolation of the plasmid DNA from bacterial clones was conducted using Zymo or Qiagen mini prep and maxi prep kits (Qiagen #27104, Zymo #D4208T)
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bioRxiv - Bioengineering 2020Quote: ... Sequence verified and purified plasmids were transformed into yeast strain W303α using the Frozen-EZ Yeast Transformation II Kit (Zymo research) according to the manufacturer’s instructions and plated on YNB-URA dropout plates ...
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bioRxiv - Microbiology 2021Quote: ... were prepared and transformed with the Y2H plasmid constructs by using the Frozen-EZ Yeast Transformation II kit (Zymo Research, USA) and following the manufacturer-supplied protocol and recommendations ...
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bioRxiv - Genomics 2021Quote: ... The yeast DNA samples were collected by thawing frozen yeast and following the standard procedure of a Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research).
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bioRxiv - Microbiology 2019Quote: ... 5 µL of ligated plasmid was then transformed into competent BW23473 cells (made using the Mix & Go E. coli Transformation Kit & Buffer Set, Zymo Research) and plated on LB + amp plates and grown overnight ...
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bioRxiv - Genomics 2021Quote: ... Clones were verified by Sanger sequencing (Quintara Biosciences) prior to retransformation and maxiprep using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Immunology 2022Quote: ... Plasmid DNA was extracted from ten million yeast from each round of selection using the Zymoprep Yeast Miniprep Kit (Zymo Research), following manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: Testing and control combinations of plasmids were co-transformed into yeast AH109 strain using the Frozen-EZ Yeast Transformation II Kit (ZYMO, T2001) and cultured on a SD/-Leu-Trp selective plate at 30 °C for 2–4 days ...
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bioRxiv - Immunology 2022Quote: DNA was obtained from at least 107 yeast per round of selection using a Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). Primers were designed to amplify the Aga2 leader peptide through to the beginning of the β2M sequence ...
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bioRxiv - Bioengineering 2022Quote: The isolated yeast populations obtained after round 4 were subjected to colony screen and plasmid recovery by using Zymoprep kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the DNA template via in vitro transcription (IVT ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Immunology 2021Quote: Overnight cultures of MACS sorted antibody-escaped and ACE2 preselected yeast populations were proceed to yeast plasmid extraction kit (Zymo Research). PCRs were performed to amplify the N26 barcode sequences as previously described13 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and cell pellets were frozen on dry ice until extraction of plasmid DNA was performed using a commercial kit (ZymoPURE Miniprep Kit, Zymo Research) and stored at -20 °C until further use.
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bioRxiv - Microbiology 2021Quote: ... as well as two replicates of sorted yeast display library based on RBD-binding and expression were extracted from sorted yeast cells using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was isolated from 10 million yeast from each round using a Zymoprep Yeast Miniprep Kit (Zymo Research; Irvine, CA). Amplicons were generated to capture the peptide through the 3C protease site ...