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Citations for Zymo Research :
451 - 500 of 501 citations for Mouse CSRNP1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: DNA associated with the mutants recovered from the fourth round of screening was isolated using a Zymoprep yeast plasmid miniprep II kit (Zymo Research). For both the TOM22 and c-Kit screens ...
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bioRxiv - Biophysics 2022Quote: ... the dsDNA target was PCR amplified from the plasmid containing the protospacer and purified by DNA Clean & Concentrator-25 (Zymo Research). The reaction was initiated with incubating 1 μM Casπ RNP effectors with 20 nM dsDNA target at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids used for transient transfections in HEK293T cells were prepared and purified using the Zymopure Midiprep Kit (Zymo Research, cat #D4201). Mutations to create the CFTR ΔPRF constructs were chosen using RNA structure predictions performed by the ViennaRNA RNAfold Web Server.
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bioRxiv - Cell Biology 2023Quote: ... Bacteria containing the constructs were then grown overnight at 37°C and the DNA was extracted using the Zyppy Plasmid Miniprep Kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2023Quote: ... Yeast transformations (plasmids and linear fragments for genomic integration) were done using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). All other chemicals were obtained from Merck unless otherwise specified.
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bioRxiv - Bioengineering 2023Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the PCR DNA template via in vitro transcription (IVT ...
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bioRxiv - Genomics 2024Quote: ... The transformed library was grown overnight in 50 mL of liquid Luria Broth (LB) + 50 μg/mL Ampicillin and purified using the ZymoPURE II Plasmid Midiprep kit (Zymo D4200) according to manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in 50-100 mL of LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE II Plasmid Midiprep Kit (Zymo #D4201) by following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE Plasmid Miniprep Kit (Zymo #D4210) by following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Synthetic Biology 2024Quote: ... Donor DNA was generated by PCR amplification from the pre-assembled donor DNA plasmids and purified by DNA Clean & Concentrator Kit (Zymo Research).
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bioRxiv - Cell Biology 2024Quote: ... Assembled plasmids (termed pTREP-ID vector hereafter) were then purified using a DNA Clean and Concentrator purification kit (Zymo, no. D4014) and transformed into One Shot ccdB Survival 2 T1R resistant competent cells (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... This was done by isolating genomic DNA of each yeast strain using the Zymoprep yeast plasmid miniprep II kit (Zymo Research) according to the manufacturer’s protocol and PCR amplifying the region of interest.
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated from 5µl of mouse serum using the RNA Clean & Concentrator -5 (Zymo) or High Pure Viral Nucleic Acid kit (Roche ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was isolated from mouse kidneys using Direct-zolTM RNA MiniPrep Plus (Zymo Research). 1 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The Carbon-Clarke equation was used to calculate how many colonies were needed to ensure a 99% confidence that all guides in the library was represented after each transformation (∼37,000 colonies).20 Colonies were scraped and midi-prepped (Zyppy Plasmid Midiprep Kit, Zymo Research, Irvine, CA) in preparation to be transformed into strain W.
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted and stored at −20°C for at least six hours prior to extraction using Zymo Yeast Plasmid Miniprep II (Zymo Research # D2004), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the LentiCRISPRv2 hygro plasmid was purified using a 0.7% agarose gel and a Zymoclean gel DNA recovery kit (Zymo Research cat # D4001). The prepared sgRNA oligo duplexes were then ligated into the purified LentiCRISPRv2 hygro plasmid ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were extracted from a 10 mL culture (5 OD) of sorted cells subpopulations using the Zymoprep™ Yeast Plasmid Miniprep II (Zymo Research) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... coli cells were recovered by centrifugation and pGFP was purified using ZymoPURE™ Plasmid Maxiprep Kit (catalog #D4203, Zymo Research, Irvine, USA). The DNA concentration was measured in a NanoDrop™ 2000/2000c Spectrophotometer (ThermoScientific® ...
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bioRxiv - Genetics 2023Quote: ... The amplicons were treated with DpnI restriction enzyme to remove the plasmid template and transformed into XL10 gold competent cells prepared by ‘Mix and Go!’ transformation kit (Zymo Research, T3001). The sequence of the isolated plasmids was confirmed using nanopore sequencing (Plasmidsaurus ...
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bioRxiv - Bioengineering 2023Quote: Wild type 4D5 and 5A12 VH and VL scFv gene fragments were purchased from Twist Bioscience and transformed into the BamHI digested pYD1 plasmid via homologous recombination (Boder and Wittrup, 1997; Chao et al., 2006) using Frozen-EZ Yeast Transformation II (Zymo Research, T2001). 4D5 scFv was transformed in a HA-VL-218-VH-FLAG orientation and 5A12 scFv as HA-VH-218-VL-FLAG ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from mouse kidneys using Direct-zol™ RNA MiniPrep Plus (Zymo Research). 2 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research), and all of them were assessed for purity and quantity using a Nanodrop 1000 spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Biochemistry 2021Quote: ... which were then grown in liquid culture prior to purification of the plasmid library using a ZymoPure II Endotoxin-Free Midiprep kit (Zymo Research, Irvine, CA). An analysis of our final DMS data suggest this library contains at least 7,614 of 7,686 possible variants ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmid DNAs were linearized by restriction enzyme digest (SpeI) and purified using the Zymo DNA Clean and Concentrator protocol (Zymo Research, Irvine, CA). In vitro transcription of mRNA was performed using the T7 mScript Standard mRNA Production System (Cellscript ...
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bioRxiv - Synthetic Biology 2020Quote: ... MCs were further cleaned to remove any PP contamination using Plasmid-Safe ATP Dependent DNase kit (Epicenter, WI, USA) followed by the DNA Clean & Concentrator Kit (Zymo Research, CA, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... High yield pure plasmid DNA preparations were subsequently obtained from 100 mL overnight LB broth cultures using the endo-free ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, USA).
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bioRxiv - Molecular Biology 2021Quote: ... pLXSP-16E7 or control vector infected primary mouse keratinocytes with the Quick-RNA Miniprep kit (Zymo Research). cDNA was synthesized with the Quantinova Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Genomics 2020Quote: Total RNA was isolated from mouse cochlea sensory epithelia using the Direct-zolTM RNA MiniPrep (Zymo Research). The RNA was quantified using a Nano-drop® (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from mouse hearts using either the Direct-zol RNA Miniprep kit (Zymo, cat# R2051) or the RNeasy Fibrous Tissue Mini Kit (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from DIV12 infected mouse cortical neurons using the Direct-zol RNA microprep (Zymo). Ten µg were reverse-transcribed into cDNA by Maxima First Strand cDNA Synthesis Kit (Thermofischer) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse hippocampus with the Quick-RNA Miniprep Plus Kit (Zymo Research, R1058) and RNA integrity was determined using Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA (400 ng) from mouse tail was bisulphite converted with the EZ DNA Methylation-Lightning kit (Zymo Research ...
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bioRxiv - Genomics 2022Quote: ... The methylation data of each brain sample was then compared with cortex aging data of mouse by Zymo’s DNAge® predictor tool as described earlier (42).
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bioRxiv - Molecular Biology 2019Quote: Total RNA was prepared from fibroblasts isolated from mouse hearts using Direct-zol RNA miniprep kit (Zymo Research). cDNA libraries were prepared using the NEBNext Ultra Directional Library Preparation Kit following rRNA depletion using the NEB rRNA depletion kit ...
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bioRxiv - Molecular Biology 2024Quote: Mouse back skin was homogenized and RNA was extracted using Quick-RNA Miniprep Kit (Zymo Research, Cat# R1054) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from mouse iBAT following the standard protocol for the Direct-zol RNA Miniprep kit (Zymo Research) and assessed for quality on the Agilent 2200 TapeStation (Agilent Technologies) ...
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bioRxiv - Systems Biology 2022Quote: Total RNA was extracted from mouse kidneys and livers by the Direct-zol™ RNA Purification Kits (Zymo Research). Purified RNA was reverse transcribed using the SuperScript™ IV First-Strand Synthesis System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: Total RNAs were purified from each group of mouse cortex part using Direct-ZolTM RNA Miniprep Plus (Zymo research) and used for reverse transcription by SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... all mouse tissues obtained from BSL3 were treated with 1% Triton by volume or placed in TRIzol (Zymo R2050-1) to inactivate virus.
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bioRxiv - Immunology 2021Quote: ... triplicate cell samples or 50 uL fresh mouse blood were harvested into Trizol reagent before purification via Direct-zol RNA miniprep (Zymo Research) per manufacturer’s instructions with an additional dry spin after disposing of the final wash to prevent carryover ...
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bioRxiv - Microbiology 2021Quote: Infected Huh-7 cells (with or without medium) or mouse lung tissues were lysed in DNA/RNA shield reagent (Zymo Research) and total RNA was extracted by using RNeasy kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... 4sU-labelled mouse and Drosophila cells were mixed and RNA was extracted using the Direct-zol DNA/RNA Miniprep kit (Zymo Research) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was isolated from a small aliquot of mixed mouse and fly cells using Quick-DNA Miniprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from human iPSC-FBs and mouse Raw 264-7 cells using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: Fecal pellets were collected for individual mouse in each group at the same day and stored in DNA/RNA shield solution (Zymo Reasearch) at −20°C until further processing ...