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Citations for Zymo Research :
1 - 50 of 104 citations for Mouse Anti Staphylococcus Aureus Enterotoxin G Antibody SEG 16 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: For long read sequencing DNA extractions of Staphylococcus aureus ST188 and selected evolved isolates were performed using the Quick-DNA™ High Molecular Weight (HMW) MagBead Kit (Zymo Research, UK) with the addition of 100 µL of 0.5 mg/mL lysostaphin (from Staphylococcus staphylolyticus ...
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bioRxiv - Neuroscience 2022Quote: ... and 16S targeted sequencing was completed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA) and V3-V4 16S primers (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene using primers custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated for 16 hours at 16 °C and were terminated by cleaning with RNA Clean & Concentrator (Zymo Research). Crosslink reversal was done by irradiating the RNA on ice with 2.5 KJ/m2 254 nm UVC using a CL-1000 crosslinker (UVP).
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bioRxiv - Immunology 2023Quote: ... Bacterial 16S rRNA gene targeted sequencing was performed using the Quick-16S NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Microbiology 2021Quote: ... 16S ribosomal RNA gene-targeted sequencing was performed using a Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA), in which 16S primers were used to amplify the V3/V4 region of the 16S rRNA gene ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA sequencing libraries were prepared by amplifying and barcoding the V1-V2 region of the 16S rRNA gene using the Quick-16S NGS Library Prep Kit (Zymo Research). Samples were pooled and sequenced on the Illumina MiSeq platform with 2 × 250 base pair standard run at Washington University DNA Sequencing Innovation Lab.
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bioRxiv - Physiology 2021Quote: ... bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S NGS Library Preparation Kit (Zymo Research, Irvine, CA). The bacterial 16S primers ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene using primers custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
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bioRxiv - Microbiology 2021Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Bacterial 16S primers amplify the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Molecular Biology 2023Quote: TM4 and C-terminal tail point mutations were made by digesting Asu-ACR-16-REM and Dme-ACR-16-REM with Bsu15I and ApaI (ThermoScientific, USA) followed by gel purification (Zymo Research, USA). These were then ligated to oligo inserts containing the TM4 and C-terminal tail point mutations (S Table 2) ...
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bioRxiv - Neuroscience 2022Quote: ... and V3-V4 16S primers (Zymo Research, Irvine, CA). The sequencing library was prepared using real-time PCR ...
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bioRxiv - Neuroscience 2022Quote: ... The DNA samples were prepared for targeted sequencing with the Quick-16S NGS library Prep kit and 16S primer set V3-V4 (Zymo Research Irvine, CA). The final library was sequenced on Ilumina MiSeq with a V3 reagent Kit (600 cycles) ...
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bioRxiv - Neuroscience 2022Quote: ... was used for bacterial 16S ribosomal RNA targeted sequencing and custom 16S primers were utilized to amplify the V3-V4 region (Zymo Research, Irvine, CA). Real-time PCR was then used to prepare the sequencing library and final qPCR fluorescence readings were pooled together according to equal molarity and the final pooled library was cleaned using the Select-a-Size DNA Clean & Concentrator™ (Zymo Research ...
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bioRxiv - Bioengineering 2024Quote: ... Primers were custom designed by Zymo Research to ensure best coverage of the 16S gene and high sensitivity - Quick-16S™ Primer Set V1-V3 (Zymo Research, Irvine, CA). The final PCR products were quantified with qPCR fluorescence readings and pooled together based on equal molarity ...
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bioRxiv - Microbiology 2021Quote: ... To characterize the microbial diversity based on 16S rRNA gene sequences we used the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine CA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and the Quick-16S™ Primer Set V3-V4 (Zymo Research). The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation ...
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bioRxiv - Neuroscience 2022Quote: ... The Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA) was used for bacterial 16S ribosomal RNA targeted sequencing and custom 16S primers were utilized to amplify the V3-V4 region (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: The 16S rRNA sequencing and analysis was performed in service by Zymo Research (Germany) ...
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bioRxiv - Microbiology 2024Quote: ... 16S rRNA gene and ITS library preparation and sequencing were performed by Zymo Research in an Illumina MiSeq platform and generating a minimum of 25,000 PE 300bp reads per amplicon type for each sample ...
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bioRxiv - Neuroscience 2021Quote: ... The primer sets used were Quick-16S™ Primer Set V3-V4 (Zymo Research). The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation ...
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bioRxiv - Neuroscience 2021Quote: The 16s rRNA sequencing and analysis was performed by a service offered by Zymo Research (Irvine ...
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bioRxiv - Microbiology 2022Quote: 16S sequencing was performed and analyzed by the ZymoBIOMICS Service (Zymo Research, Irvine, CA). Zymo Research performed library preparation ...
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bioRxiv - Bioengineering 2023Quote: ... The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research) supplemented with RNase A (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were incubated overnight at 16°C and were terminated using RNA Clean & Concentrator (Zymo Research). Crosslink reversal was done by irradiating the RNA with 2.5 KJ/m2 254 nm UVC using a CL-1000 crosslinker (UVP ...
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bioRxiv - Bioengineering 2023Quote: ... and 600 µl of g-DNA Wash buffer (Zymo Research) were added with intermediate centrifugation steps for 1 min at 10000 x g ...
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bioRxiv - Genomics 2019Quote: Purified DNA was bisulfite converted following the T-WGBS protocol[16] with the EZ DNA methylation kit (Zymo). Oligonucleotide replacement was performed by incubating 9 μl of tagmented M-ATAC or M-ChIP purified DNA with 2 ng of phage lambda DNA as carrier ...
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bioRxiv - Genetics 2023Quote: ... individual array bearing worms were isolated and lysed in 4µl of EB (Zymo, Cat. No.: D3004-4-16) buffer with 1mg/ml proteinase K (NEB) ...
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bioRxiv - Pathology 2023Quote: ... The swabs were analyzed through sequencing of the 16S rDNA gene undertaken by ZymoBIOMICS (Zymo Research, Irvine, CA). DNA was extracted from swabs using ZymoBIOMICS-96 MagBead DNA kit (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... DNA samples were prepared for targeted sequencing with the Quick-16S™ NGS Library Prep Kit (Zymo Research) and the Quick-16S™ Primer Set V3-V4 (Zymo Research) ...
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bioRxiv - Immunology 2022Quote: ... 16S bacterial rDNA was isolated from stool and intestinal contents with a ZymoBIOMICS DNA kit (Zymo Research, Irvine, CA) kit ...
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bioRxiv - Neuroscience 2021Quote: The DNA samples were prepared for targeted sequencing with the Quick-16S™ NGS Library Prep Kit (Zymo Research). The primer sets used were Quick-16S™ Primer Set V3-V4 (Zymo Research) ...
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bioRxiv - Genomics 2024Quote: All nucleic acid extraction for 16S rRNA amplicon sequencing was performed using ZymoBIOMICS DNA/RNA Miniprep Kit (Zymo Research) and stored at −20 °C until further processed ...
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bioRxiv - Biochemistry 2023Quote: ... and Zymo-Spin IIICG columns (Zymo Research, catalog #C1006-50-G) as per the manufacturer’s instructions with a minor change in the flowthrough step ...
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bioRxiv - Developmental Biology 2021Quote: Three embryos at neural plate (st. 16-18) or three dissected heads at larval (st. 28-34) stages were collected in TRI-reagent (Zymo) and processed for RNA extraction with DNAse I treatment using the Direct-zol RNA Miniprep kit (Zymo) ...
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bioRxiv - Plant Biology 2021Quote: ... Lysis tubes were shaken 5 min at 16 Hz using a ball mill to extract and purify DNA following the manufacturer’s guidelines (Zymo Research). Microbiome profiling of isolated DNA samples was performed by Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2019Quote: ... qPCR was used to quantify 16S rRNA gene abundances [40] using the Femto™ Bacterial DNA Quantification Kit (Zymo Research). Assays were conducted following manufacturer instructions using a BioRad CFX Connect™ Real-Time PCR Detection System ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA samples were prepared for targeted sequencing with the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Primers were custom designed by Zymo Research to ensure best coverage of the 16S gene and high sensitivity - Quick-16S™ Primer Set V1-V3 (Zymo Research ...
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bioRxiv - Genomics 2020Quote: ... DNA in the initial 20-μL nucleic acids solution was digested by adding 16-μL DNase I (1 U/μL) plus 4-μL 10X DNase I buffer (DNase I set; Zymo Research), incubating for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were made from the Zymo mock community using the LoopSeq 16S long-read kit (referred to throughout as ‘Zymo-Loop’). This preparation was also followed for the ATCC-gut and ATCC-oral communities as well as the uterine lavage and cyanobacterial enrichment samples ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA extracted from individual particle-attached communities was quantified through qPCR of the 16S rRNA gene using the Femto Bacterial DNA Quantification Kit (Zymo Research), which has a lower limit of detection of 20fg ...
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bioRxiv - Developmental Biology 2021Quote: ... Sheared DNA was ligated with methylated adaptors (MGIEasy WGBS Adapters-16, MGI) and subjected to bisulfite conversion using the EZ DNA Methylation-gold kit (Zymo Research). Bisulfite- converted DNA was amplified with 13 PCR cycles and purified by AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... a sequencing library that captured the V3V4 hypervariable region of the 16S rRNA gene was made from the Zymo mock community (named ‘Zymo-V3V4’). To generate this comparison library ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM CaCl2 or both chemicals for 16 h (see Plant materials, growth and stress assays) using the ZR Plant RNA MiniPrep Kit (Zymo Research). RNA sequencing was performed at The Mantoux Institute for Bioinformatics of the Nancy and Stephen Grand Israel National Center for Personalized Medicine ...
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bioRxiv - Pathology 2023Quote: ... The DNA samples were then prepared for targeted sequencing with Quick-16S Primer Set V3-V4 Library (Zymo Research, Irvine, CA). The sequencing library was prepared using innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and limit PCR chimera formation ...
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bioRxiv - Molecular Biology 2022Quote: ... G/I nucleotide-tailed RNA was then purified using spin columns (Zymo Research). The RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was reverse-crosslinked overnight (12-16 hours) at 65°C prior to ChIP DNA isolation using Zymogen ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205). For ChIP-seq ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal volumes of fractions 10-16 were pooled and RNA was by acidic phenol chloroform extraction followed by column purification (Zymo Research, R1013) as described above ...
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bioRxiv - Genomics 2023Quote: Reads were aligned to the 16S genes extracted from the complete reference genomes of the bacteria present in the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research D6305) using minimap2 ...
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...