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Citations for Zymo Research :
101 - 150 of 173 citations for Mouse Anti Hepatitis C Virus NS4b Antibody 1867 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
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bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged 1 min at 12000 g at 4°C and RNAs were extracted from supernatants with Direct-zol RNA Mini Prep kit (Zymo Research), following manufacturer’s protocol and including the optional DNAse treatment step ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were incubated for 1 hour at 37 °C and DNA was purified using the DNA Clean & Concentrator-25 kit (Zymo Research) as described by the manufacturer.
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bioRxiv - Genomics 2021Quote: ... The mixture was incubated at 37°C for 30 minutes and subsequently purified using the ChIP DNA purification kit (Zymo Research) as described by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... The extracted RNA was treated with DNase I for 15 minutes at 37°C and further purified using a Quick-RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).
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bioRxiv - Microbiology 2022Quote: ... were incubated in a total volume of 20 µL for 30 min at 37°C and subsequently column-purified using RNA Clean and concentrator 5 (Zymo Research).
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bioRxiv - Microbiology 2023Quote: ... The reaction was incubated at 32°C for 4 hours before being treated with DNase for 20 minutes at 37°C then purified using an RNA clean and concentrate kit (Zymo Research). The RNA quality was checked using a MOPS/formaldehyde agarose gel electrophoresis.
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bioRxiv - Molecular Biology 2023Quote: ... Samples were either resuspended in 30 µL 100% RNALater and stored at -70°C or resuspended in 100 µL TRI reagent (Zymo Research). Samples were crushed with a micropestle and RNA was extracted using a Direct-zol RNA MicroPrep kit (Zymo Research).
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bioRxiv - Immunology 2023Quote: ... incubated for 15 min at room temperature and either frozen at -80°C or directly followed by RNA isolation using a Direct-zol RNA microprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Two hundred and fifty nanograms of linker-ligated genomic DNA were subjected to sodium bisulfite conversion for 210 min at 64°C using the EZ DNA Methylation Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The RNAse treatments were carried out for 20 min at 30°C followed by RNA extraction using DirectZolTM (Zymo Research®) kits as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... The lysates were centrifuged at 5000 x g for 10 min at 4 °C and supernatant added to gDNA extraction columns (Zymo Research). The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research ...
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bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria containing the constructs were then grown overnight at 37°C and the DNA was extracted using the Zyppy Plasmid Miniprep Kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at 37°C for 1 hour and then purified with Zymo DNA Clean & Concentrator-5 (Zymo Research) following the PCR product protocol and eluting DNA into 10.5 μL of water ...
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bioRxiv - Molecular Biology 2023Quote: ... for 30 min at 37°C and further purified by using a Zymo DNA Clean and Concentrator column (Zymo Research, D4033).
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bioRxiv - Microbiology 2023Quote: ... Prototrophic colonies were grown in uracil dropout medium (MilliporeSigma) at 30 °C and 250 rpm and the plasmid was purified using the Yeast Plasmid MiniPrep II Kit (Zymo Research). The plasmid was transformed into TransforMax EPI300TM E ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× RNase H buffer overnight at 37 °C before immunoprecipitation and recovered using the Oligo Clean & Concentrator kit (Zymo Research). For m1A-DIP ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with 1.25 units of RQ1 DNase for 30 minutes at 37°C and RNA recovered using an RNA Clean & Concentrator-25 spin column kit (Zymo Research), following manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... at 37°C for 1 hr and purified again by illustra microspin G-50 column or Direct-zol RNA Miniprep kits (Zymo Research) before transfection (Supplemental Fig ...
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bioRxiv - Genetics 2023Quote: ... Transposition reactions were incubated for 30 min at 37°C while shaking at 1,000 rpm and cleaned using DNA Clean & Concentrator-5 (Zymo Research, D4003). Transposed libraries were amplified in 50 μl PCRs (0.5 μl Q5 DNA polymerase ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was reverse-crosslinked overnight (12-16 hours) at 65°C prior to ChIP DNA isolation using Zymogen ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205). For ChIP-seq ...
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bioRxiv - Genomics 2022Quote: ... The corresponding amount of polyA RNA were incubated with 1X fragmentation buffer at 95 °C for 1 minute to generate ~300 nt of RNA fragment followed by column purification (Zymo Research, R1016) as described before [23] ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted and stored at −20°C for at least six hours prior to extraction using Zymo Yeast Plasmid Miniprep II (Zymo Research # D2004), following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... for 1 h at 37°C to remove free oligonucleotide and purified by ethanol precipitation/ or the DNA Clean and Concentrator 5 kit (Zymo Research, D4003T). Libraries were resolved on a 2% MetaPhor agarose (Lonza ...
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 10 min at 37°C before quenching using a Zymo Genomic DNA Clean & Concentrator 10 (Zymo Research D4011) kit according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The transcribed product was treated with DNase I for 30 min at 37°C and then purified using RNA Clean and Concentrator Kit (Zymo Research #R1016).
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bioRxiv - Cell Biology 2023Quote: ... The crosslinking was reversed at 65 °C and the samples were purified using the ChIP DNA Clean and Concentrator kit (Zymo Research, D5205). The DynaMagTM-2 Magnet (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were fragmented to around 300nt length at 95°C for 1min and purified using RNA clean and concentrator (Zymo research, R1016). The fragmented RNA was then subjected to a 5’ dephosphorylation reaction at 37°C for 30min by adding rSAP (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
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bioRxiv - Genomics 2023Quote: ... Single colonies were used to inoculate ampicillin liquid cultures grown overnight with shaking at 37°C and plasmids were subsequently purified with the ZR Plasmid Miniprep kit (Zymo Research #D4016). The purified plasmids were screened by Sanger sequencing (GeneWiz ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mL of an overnight culture of EC1000 grown in LB media at 37°C was transferred to 50 mL of ZymoBroth (Zymo Research #M3015) and cultured for 15–16 hours at 22°C with shaking to obtain a culture of OD600=0.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... pLXSP-16E7 or control vector infected primary mouse keratinocytes with the Quick-RNA Miniprep kit (Zymo Research). cDNA was synthesized with the Quantinova Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Genomics 2020Quote: Total RNA was isolated from mouse cochlea sensory epithelia using the Direct-zolTM RNA MiniPrep (Zymo Research). The RNA was quantified using a Nano-drop® (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from mouse hearts using either the Direct-zol RNA Miniprep kit (Zymo, cat# R2051) or the RNeasy Fibrous Tissue Mini Kit (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from DIV12 infected mouse cortical neurons using the Direct-zol RNA microprep (Zymo). Ten µg were reverse-transcribed into cDNA by Maxima First Strand cDNA Synthesis Kit (Thermofischer) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse hippocampus with the Quick-RNA Miniprep Plus Kit (Zymo Research, R1058) and RNA integrity was determined using Agilent Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: MSP1-specific B cells that successfully expanded in culture were collected by centrifugation (5 min. at 250 × g and RT) and stored at -70°C in 50 µl Tri-Reagent (Zymo #R2050-1-200). Heavy and light chain variable regions were amplified from MSP1-specific B cells by cDNA synthesis and a series of PCR reactions ...
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bioRxiv - Microbiology 2020Quote: The respiratory samples were prospectively stored at −80°C after stabilization by mixing an equal volume of DNA/RNA Shield (2X concentrate; Zymo Research, Irvine, CA). The thawed samples were centrifuged at 20,000×g for 2 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Total RNA from archived (stored at -80°C) American alligator whole blood was isolated using the Quick-RNA Whole Blood Kit (Zymo Research, Irvine, CA). Samples from both LW and CFR were selected randomly based only on the availability of samples ...
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bioRxiv - Microbiology 2021Quote: ... Each strain was grown in 1 ml of LB+CA100 at 30°C for 48 hours and genomic DNA was extracted using a Quick-DNA High Molecular Weight kit (Zymo Research, cat#D6060) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... chromatin was de-crosslinked for 15 minutes at 65°C and purified using the ChIP DNA Clean & Concentrator kit (Zymo Research, Freiburg, Germany). Binding of Msh2 ...
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bioRxiv - Microbiology 2021Quote: ... The pellet was frozen at -20°C until DNA extraction using a Zymo fecal/soil DNA extraction kit according to enclosed directions (Zymo Research, Irvine, CA). After sonication ...
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bioRxiv - Microbiology 2020Quote: ... isolates were grown shaking in SG broth at 37°C and genomic DNA isolated using the Quick-DNA Fungal/Bacterial miniprep kit (Zymo Research, Irvine, CA) according to the manufacturer's suggested protocol.
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA (400 ng) from mouse tail was bisulphite converted with the EZ DNA Methylation-Lightning kit (Zymo Research ...
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bioRxiv - Genomics 2022Quote: ... The methylation data of each brain sample was then compared with cortex aging data of mouse by Zymo’s DNAge® predictor tool as described earlier (42).