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Citations for Zymo Research :
101 - 150 of 246 citations for Monobenzyl Phthalate Unlabeled 100 Ug Ml In Methyl Tert Butyl Ether since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Samples were either resuspended in 30μL 100% RNALater and stored at -70°C or in 100μL TRI reagent (Zymo Research). Samples were crushed with a micropestle and RNA was extracted using a Direct-zol RNA MicroPrep kit (Zymo Research) ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA (100 ng per sample) was treated with sodium bisulfite using the DNA Methylation Gold Kit (Zymo Research). The bisulfite-treated DNA was PCR-amplified with TaKaRa EpiTaq HS (TaKaRa Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... 600μL aqueous phase was mixed with 600 μL 100% ethanol and RNA was purified on silica columns (Zymo, R1013).
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bioRxiv - Molecular Biology 2023Quote: ... and 150 µL of 100% ethanol and then purified through a column from the purification kit (Zymo Research, R1015) followed by quality check with a Bioanalyzer small RNA kit (Agilent).
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from 100 µl of lysates prepared for ribosome profiling using Direct-zol mini kit (Zymo) or Trizol reagent (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... 1.5 ml of Lysis Buffer (ZR-DuetDNA/RNA Miniprep Plus, Zymo Research) was added for 15 minutes at room temperature (around 35°C) ...
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bioRxiv - Plant Biology 2020Quote: RNA was extracted from a total of 100 mg of ground frozen shoot tissue with Direct-zol kit (Zymo Research) according to manufacturer’s protocol with in-column DNAse treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... Eluates were then concentrated in a SpeedVac to ∼100 µL and purified with the DNA Clean and Concentrator Kit (Zymo). Libraries were sequenced on a HiSeq 2000 at the BioMicroCenter Core Facility at MIT with 50 nt read length.
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bioRxiv - Microbiology 2019Quote: ... The filter-associated biofilm was disrupted by adding 1 ml PBS + 0.01% Triton X-100 detergent and agitating the tubes at high speed for 2 minutes using a Genie Disruptor (Zymo). This suspension was diluted ...
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bioRxiv - Developmental Biology 2022Quote: ... The DNA digestion efficiency was monitored by using 100 ng 5-methylcytosine and 5-hydroxymethylcytosine DNA standard sets (Zymo Research) in parallel under identical conditions ...
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bioRxiv - Molecular Biology 2019Quote: 100 ng of bisulphite-converted DNA was used as template for the PCR using the ZymoTaq DNA polymerase (Zymo Research). The reaction was carried out in a total volume of 40 µl in the reaction buffer provided by the manufacturer supplemented with of 1 mM dNTPs ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted from either 100 µl of ribosome footprinting lysate with the RNA Clean & Concentrator-25 kit (Zymo, R1017), or directly from cells using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was combined with an equal volume of 95-100% ethanol and transferred to a RCC5 column part of the RNA Clean and Concentrator kit from Zymo Research and manufacturer’s instructions were followed.
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bioRxiv - Microbiology 2022Quote: ... Filters were then transferred into a 1.5 ml tube and the filter-associated cells were resuspended by adding 1 ml 1X PBS + 0.05% Triton X-100 detergent and agitating the tubes at high speed for 2 minutes using a Genie Disruptor (Zymo). This suspension as well as the inoculum were diluted ...
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bioRxiv - Microbiology 2021Quote: ... Equal volume of 100% EtOH was mixed with the TRIzol homogenate and processed using Direct-Zol RNA MiniPrep Kit (Zymo) to extract viral RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction volume was increased to 100 μl with H2O and further purified using RNA Clean & Concentrator (Zymo Research, R1013) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... Total RNA was extracted from 100 mg tumor tissue using the Direct-zol RNA Miniprep kits (Zymo Research, Cat # R2053) then reverse transcribed with cDNA Reverse Transcription Kit (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... the cells were pelleted and resuspended at 1x106 cells per 300 ul of RNA lysis buffer (Zymo, #R1060-1-100). Cells were pipette mixed and frozen at -80 until RNA isolation was performed ...
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bioRxiv - Genomics 2023Quote: ... 100 ng of sheared DNA was bisulphite-converted using the EZ DNA Methylation-Gold Kit (D5005; Zymo Research, Irvine, CA). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... a small piece of gel (∼100 μl volume) was dissolved in 400 μl TRI-reagent (Zymo Research, R2050-1-200) and the RNA was purified using an RNA isolation kit (Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... the aqueous phase was transferred to a fresh tube and 1 volume of 100% ethanol was added and mixed thoroughly before binding to an RNA Clean & Concentrator (Zymo). RNA was DNase I digested on-column (Zymo) ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were cleaned up with RNA Clean and Concentrator 100 columns (Zymo, referred to as “kit-purified” in text). Purity was assessed using a 6% Tris-Borate-Urea gel (Invitrogen).
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bioRxiv - Developmental Biology 2021Quote: ... and supplemented with 1mg/mL Proteinase K (Zymo Research, Cat#: D3001-2-5) for 1 hour at 55°C ...
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bioRxiv - Microbiology 2023Quote: ... which were initially lysed by adding 1 ml of TRI Reagent (Zymo Research) and subsequently processed with the Direct-zol RNA Miniprep Plus kit (Zymo Research ...
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bioRxiv - Biochemistry 2023Quote: ... and 10% SDS) and 1 µl Proteinase K (20 mg/ml; Zymo Research) were added to stop the DNA digest and start the protein digest to free the ssDNA from the AAV particles ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were directly lysed in 1 ml of Trizol (R2050-1, Zymo Research) and subjected to interface cleanup as described below.
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bioRxiv - Synthetic Biology 2024Quote: ... and 10 % SDS) and 1 μl Proteinase K (20 mg/ml; Zymo Research) were added inhibiting the Benzonase and digesting proteins including the AAV capsid to free the ssDNA ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 1 ml of sediment slurry was serially diluted and 100 μL was plated in 10−4 dilution in triplicate on MB2216 agar plates using rattler beads (Zymo #S1001); colony forming units (CFU ...
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bioRxiv - Cell Biology 2021Quote: ... Spheroplasting of harvested yeast cells was performed for 2 minutes in lysis buffer (1 M sorbitol 100 mM EDTA, 0.1% β-mercaptoethanol) containing 25 units of zymolyase (Zymo Research). RNA extraction was performed with an RNeasy kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: Viral particles were collected from virus-infected A549 hACE2 cell supernatants at 72 hours post infection (100 μL) and viral RNAs were isolated from the supernatants using the Quick RNA Viral 96 Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
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bioRxiv - Genetics 2022Quote: ... The aqueous layer from the chloroform extraction was combined with an equal volume of 100% ethanol and transferred to a Zymo-Spin IC column (Zymo Research). Columns were centrifuged for 30 s at 16,000 RCF ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 µL of this soma sample was then carried forward for RNA isolation using the Zymo QuickRNA MicroPrep kit (Zymo R1050). In parallel ...
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bioRxiv - Genomics 2020Quote: ... 4-SUTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAseq reaction to eliminate traces of genomic DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μL of this soma sample was then used for RNA isolation using the Zymo QuickRNA MicroPrep kit (Zymo Research, #R1050). RNA was also isolated from the 500 μL neurite lysate using the same kit ...
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bioRxiv - Microbiology 2021Quote: The extracted DNA (100-1000 ng) from anal cells was bisulfite conversion by using EZ kit Gold Bisulfite Conversion Kit (Zymo Research) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA for short-read sequencing was extracted from 80-100 mg of fungal tissue with the Fungal/Bacterial Microprep kit (Zymo; www.zymo.com). Paired-end libraries were prepared and sequenced using the Illumina HiSeq X (150-bp-long ...
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bioRxiv - Immunology 2021Quote: ... 5µl of each reaction including a negative control without scFv was subsequently transformed into 100 µl Fast Transformation of Mix & Go Competent Cells (Zymo T3001) each and plated on LB plates containing Ampicillin ...
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bioRxiv - Developmental Biology 2022Quote: ... Gel band within 100-250bp was cut and purified using Zymoclean Gel DNA recovery kit/ DNA Clean & Concentrator-5 (Zymo Research). If needed ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μL each reaction was transformed into 100 μL DH10B and transformed according to the Mix and Go Transformation kit (Zymo Research).
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bioRxiv - Bioengineering 2023Quote: ... or a modified pNTA vector with V5 tag into EBY-100 yeast using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). Transformed yeast were passaged once in minimal glucose medium (SDCAA ...
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bioRxiv - Microbiology 2024Quote: ... The total microbe DNA from 100 mg of bulk or rhizosphere samples was extracted using the Quick-DNATM Fecal/Soil Microbe Miniprep kit (Zymo Research) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... aqueous phase containing cfRNAs was mixed with 1 volume of ethanol (95-100%) and then added to the Zymo-Spin column (Zymo, R1016) for RNA binding ...
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bioRxiv - Microbiology 2024Quote: ... Samples were extracted with 100 μl of chloroform (Fisher C298-500) and the aqueous phase was processed on a Zymo column (Zymo R1018). 300 ng of each sample was loaded on Hybond-N+ membrane (Amersham RPN119B) ...
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bioRxiv - Cancer Biology 2024Quote: ... The soluble fraction was mixed with equal volume of 100% ethanol and RNA was isolated using the Direct-zol RNA Miniprep Kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cell pellet was resuspended in 750 mL of BashingBead Buffer (Zymo Research Corp.) and transferred to BashingBead Lysis Tubes (Zymo Research Corp. ...
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bioRxiv - Genomics 2022Quote: ... in presence of 1.5 ml of DNA/RNA shield (Zymo Research, Irvine, CA, USA) was thawed and disrupted by the simultaneous multidirectional striking using a high-speed homogenizer FastPrep-24 5G Instrument (MP Biomedicals ...
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bioRxiv - Genomics 2022Quote: ... in presence of 1.5 ml of DNA/RNA shield (Zymo Research, Irvine, CA, USA) and stored at −20 °C until RNA purification.
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bioRxiv - Genomics 2022Quote: ... Each 200 mL of culture was purified in Endotoxin Free MaxiPrep Reactions (Zymo #D4203) and eluted in 0.4 mL elution buffer per column.