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Citations for Zymo Research :
151 - 200 of 271 citations for Inositol monophosphatase 1 IMPA1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were run on 1% agarose gel and purified using the Zymoclean gel DNA recovery kit (Zymo Research).
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bioRxiv - Microbiology 2022Quote: ... RNA extractions were performed from 1 ml LB culture using the Direct-zol RNA MiniPrep Plus (Zymo Research, ZR2073). Total RNAs were reverse-transcribed into cDNAs using iScript™ cDNA Synthesis Kit (BioRad ...
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bioRxiv - Systems Biology 2022Quote: ... The flow through was mixed with 1 volume of ethanol then transferred to a Zymo Spin IIC column (Zymo) and centrifuged ...
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bioRxiv - Biochemistry 2020Quote: ... The entire PCR reaction is run on 1% agarose gel and the DNA at 230bp was purified (Zymo Research). See Supplemental Figure S2.
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bioRxiv - Immunology 2021Quote: ... Cxcl1 promoter regions #1 and #2 were PCR amplified from sodium bisulfite converted gDNA using ZymoTaq Polymerase (Zymo Research) and purified by gel extraction using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae eby100 was retransformed using 1 µg of pGal1 – Aga2p_eGFP following the protocol of the EZ yeast transformation kit II (Zymo). 100 µL of cells were selected on SDCAA plates ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant and pellet samples on non-denaturing 1% agarose gel we extracted RNA with TRI reagent® (ZYMO Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant and pellet samples on non-denaturing 1% agarose gel we extracted RNA with TRI reagent® (ZYMO Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL ...
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bioRxiv - Plant Biology 2023Quote: RNA was purified from seedlings Col-0 and cpk32-1 using the Direct-zol RNA MiniPrep Kit (Zymo Research). The reverse-transcriptase cDNA synthesis reaction was carried out by the RevertAid Reverse Transcriptase (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: Bacterial RNA was extracted from ∼1 × 108 CFU after AMP exposure (above) using Direct-zol RNA Miniprep kit (Zymo) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... One of each type of swabs was placed into 1 mL DNA/RNA Shield (Zymo Research, Irvine California, USA) and the other of each into 1 mL methanol ...
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bioRxiv - Microbiology 2022Quote: ... 1(10)6 lymphocytes were harvested into an equal volume of Trizol and DNA/RNA shield (Zymo Research R110-250). Total RNA was extracted using using Zymo Directzol Microprep (Zymo Research R2060 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Then 1 mL of the culture was removed for DNA extraction using the Zymoprep yeast Plasmid Miniprep II (Zymo Research) kit ...
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bioRxiv - Bioengineering 2020Quote: ... at 37°C for 1 hour to remove the gblock template then purified using a RNA spin column (Zymo Research). Purified RNA was then treated with 200 units of calf intestinal phosphatase (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: 1 μg of genomic DNA was hydrolyzed overnight at 37 ºC with 5 U of DNA Degradase Plus (Zymo Research) following the manufacturer’s protocol in 50 μl volume ...
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bioRxiv - Synthetic Biology 2021Quote: For changing cation concentration from 1 to 0 we utilized the ssDNA clean-up kit (ssDNA/RNA clean/concentrator D7010) from Zymo Research such that all the extended ssDNA synthesized in the initial part of the experiment was retained on the column and the TdT ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pQE60::trpCp variants were transformed into the ΔtrpD JW1255-1 strain (BW25113) from the KEIO collection 47 using the Mix & Go! protocol from Zymo Research (Zymo Research ...
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bioRxiv - Systems Biology 2021Quote: ... at which point a 200 µL aliquot was removed and preserved in 1 mL of DNA/RNA Shield (Zymo Research) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Metagenomic libraries were diluted to 1 nM using the Select-a-Size DNA Clean and Concentrator MagBead Kit (Zymo Research) and pooled for further sequencing on the Illumina MiniSeq platform ...
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bioRxiv - Genomics 2022Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted by vortexing for 30 min in elution buffer (1% SDS and 0.1 M NaHCO3) and DNA was purified using a ChIP DNA Clean & Concentrator kit (Zymo Research). For each ChIP ...
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bioRxiv - Synthetic Biology 2020Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Biophysics 2021Quote: ... Sufficient amounts of DNA were obtained growing 1 L of transfected XL1 Blue Escherichia coli cells overnight and performing a gigaprep (Zymo). For protein expression ...
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bioRxiv - Evolutionary Biology 2019Quote: 208 female beetles (Table 1) p stored at −80°C were homogenized in Tri-Reagent® (Zymo Research, California, USA) using an electric bead mill ...
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bioRxiv - Genomics 2021Quote: Total RNA was purified in duplicate from 1 million frozen cells using the Direct-zol RNA MiniPrep kit from Zymo Research (#R2050) ...
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bioRxiv - Genetics 2020Quote: ... total RNA from approximately 1 × 108 cells was extracted using the Quick-RNA Fungal/Bacterial Miniprep (Zymo Research, Irvine, CA). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were vortexed for 10 seconds in 300 μL of 1 × DNA/RNA Shield™ (Zymo Research, Cat. No. R1100) and centrifuged for 5 minutes at 5,000 × g ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and DNA was extracted at days 1 and 5 utilizing the Quick-DNA/RNA Miniprep (Zymo Research). Viral genomes were quantified using a standard curve and normalized to RNAseP.
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bioRxiv - Molecular Biology 2023Quote: ... PCR product sizes were verified on a 1% agarose gel prior to PCR clean up (DNA Clean & Concentrator-5, Zymo). A DNA fragment for transcription of lpp (lpp sequence preceded by aforementioned T7 transcription-enabling sequence ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
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bioRxiv - Biochemistry 2023Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Genomics 2023Quote: ... the cells were pelleted and resuspended at 1x106 cells per 300 ul of RNA lysis buffer (Zymo, #R1060-1-100). Cells were pipette mixed and frozen at -80 until RNA isolation was performed ...
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bioRxiv - Molecular Biology 2024Quote: ... the aqueous phase was transferred to a fresh tube and 1 volume of 100% ethanol was added and mixed thoroughly before binding to an RNA Clean & Concentrator (Zymo). RNA was DNase I digested on-column (Zymo) ...
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bioRxiv - Genomics 2024Quote: ... tagmentation was performed at 55°C for 5 min after the addition of 1/50uL of N7-adaptor loaded Tn5 and subsequent quenching with DNA binding buffer (Zymo) for 5 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... a small piece of gel (∼100 μl volume) was dissolved in 400 μl TRI-reagent (Zymo Research, R2050-1-200) and the RNA was purified using an RNA isolation kit (Zymo Research ...
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bioRxiv - Plant Biology 2019Quote: ... at 50°C for 1 h and RNA was extracted from input and pellet fractions using TRIzol and RNA Clean and Concentrator Columns (Zymo Research) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... These sorted libraries were grown for 1 day at 30°C shaking in SD-CAA media and then 300 µl of the cultures were miniprepped (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... DNA was then extracted from approximately 1 ml of the red blood cell pellets using Zymo Bead Genomic DNA kit (Zymo Research) following the manufacturer’s procedures ...
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bioRxiv - Genomics 2020Quote: ... We plated 1% of the library to estimate complexity and grew the rest of the sample and then midi prepped (Zymo D4200) the resulting library.
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bioRxiv - Genomics 2020Quote: ... DNA in the initial 20-μL nucleic acids solution was digested by adding 16-μL DNase I (1 U/μL) plus 4-μL 10X DNase I buffer (DNase I set; Zymo Research), incubating for 15 min at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... fine forceps were used to manually remove each neuron. Identified neurons (Fig. 1) were immediately placed in a cryogenic microcentrifuge tube containing 400 µL lysis buffer (Zymo Research) and stored at −80°C until RNA extraction ...
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bioRxiv - Plant Biology 2019Quote: ... RNA was isolated from Tak-1 and Tak-2 whole-plant material using the Direct-Zol RNA Mini-Preparation Kit (Zymo Research). cDNA synthesis was carried out using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The Variovorax CL14 genes contained in Vectors 1 and 2 were inferred by isolating plasmid from these clones using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research) and Sanger sequencing the insert ends using primers JMC247 and JMC270 (Supplementary Table 19) ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR products were subjected to 1% agarose gel electrophoresis and they were purified using a Zymogen gel DNA recovery kit (Zymo Research). The assembly reactions were transformed to chemically competent E ...
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bioRxiv - Cancer Biology 2021Quote: Genomic DNA was extracted from GBM-DCs and TRA-1-81+/SSEA4+ ic-GSCs using the Quick-DNA Microprep Kit (D-3020, Zymo Research). Bisulfite conversion ...
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bioRxiv - Cell Biology 2021Quote: ... Spheroplasting of harvested yeast cells was performed for 2 minutes in lysis buffer (1 M sorbitol 100 mM EDTA, 0.1% β-mercaptoethanol) containing 25 units of zymolyase (Zymo Research). RNA extraction was performed with an RNeasy kit (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was extracted from 1 ml breastmilk using Quick-DNA Fungal/Bacterial extraction kit following the manufacturer’s instructions (Zymo Research, USA). Extraction kits were purchased separately for Batch 1 and Batch 2 ...