Labshake search
Citations for Zymo Research :
451 - 487 of 487 citations for Human NID1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: DNA was obtained from at least 107 yeast per round of selection using a Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). Primers were designed to amplify the Aga2 leader peptide through to the beginning of the β2M sequence ...
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bioRxiv - Bioengineering 2022Quote: The isolated yeast populations obtained after round 4 were subjected to colony screen and plasmid recovery by using Zymoprep kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the DNA template via in vitro transcription (IVT ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Immunology 2021Quote: Overnight cultures of MACS sorted antibody-escaped and ACE2 preselected yeast populations were proceed to yeast plasmid extraction kit (Zymo Research). PCRs were performed to amplify the N26 barcode sequences as previously described13 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and cell pellets were frozen on dry ice until extraction of plasmid DNA was performed using a commercial kit (ZymoPURE Miniprep Kit, Zymo Research) and stored at -20 °C until further use.
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bioRxiv - Microbiology 2021Quote: ... as well as two replicates of sorted yeast display library based on RBD-binding and expression were extracted from sorted yeast cells using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was isolated from 10 million yeast from each round using a Zymoprep Yeast Miniprep Kit (Zymo Research; Irvine, CA). Amplicons were generated to capture the peptide through the 3C protease site ...
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bioRxiv - Bioengineering 2019Quote: DNA associated with the mutants recovered from the fourth round of screening was isolated using a Zymoprep yeast plasmid miniprep II kit (Zymo Research). For both the TOM22 and c-Kit screens ...
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bioRxiv - Biophysics 2022Quote: ... the dsDNA target was PCR amplified from the plasmid containing the protospacer and purified by DNA Clean & Concentrator-25 (Zymo Research). The reaction was initiated with incubating 1 μM Casπ RNP effectors with 20 nM dsDNA target at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids used for transient transfections in HEK293T cells were prepared and purified using the Zymopure Midiprep Kit (Zymo Research, cat #D4201). Mutations to create the CFTR ΔPRF constructs were chosen using RNA structure predictions performed by the ViennaRNA RNAfold Web Server.
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bioRxiv - Cell Biology 2023Quote: ... Bacteria containing the constructs were then grown overnight at 37°C and the DNA was extracted using the Zyppy Plasmid Miniprep Kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2023Quote: ... Yeast transformations (plasmids and linear fragments for genomic integration) were done using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). All other chemicals were obtained from Merck unless otherwise specified.
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bioRxiv - Biophysics 2024Quote: ... This was done by isolating genomic DNA of each yeast strain using the Zymoprep yeast plasmid miniprep II kit (Zymo Research) according to the manufacturer’s protocol and PCR amplifying the region of interest.
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bioRxiv - Cell Biology 2024Quote: ... Assembled plasmids (termed pTREP-ID vector hereafter) were then purified using a DNA Clean and Concentrator purification kit (Zymo, no. D4014) and transformed into One Shot ccdB Survival 2 T1R resistant competent cells (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE Plasmid Miniprep Kit (Zymo #D4210) by following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... Donor DNA was generated by PCR amplification from the pre-assembled donor DNA plasmids and purified by DNA Clean & Concentrator Kit (Zymo Research).
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the PCR DNA template via in vitro transcription (IVT ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in 50-100 mL of LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE II Plasmid Midiprep Kit (Zymo #D4201) by following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The transformed library was grown overnight in 50 mL of liquid Luria Broth (LB) + 50 μg/mL Ampicillin and purified using the ZymoPURE II Plasmid Midiprep kit (Zymo D4200) according to manufacturer’s instructions.
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bioRxiv - Genomics 2019Quote: ... RNA was isolated from different number of murine splenic CD3 positive T cell samples and human oocyte samples by using the Direct-zol™ RNA MiniPrep Kit (ZYMO, cat. R2050). The extracted RNA samples were dissolved into 30 µl of nuclease-free water (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... Two representative loci per fungal strain and the human MLH1 for the control were amplified by PCR (ZymoTaq PreMix, Zymo Research, Irvine, CA) using specifically designed primers for amplification of the bisulfite-treated DNA (designed using the ‘Bisulfite Primer Seeker’ (Zymo Research ...
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bioRxiv - Biochemistry 2020Quote: ... The Carbon-Clarke equation was used to calculate how many colonies were needed to ensure a 99% confidence that all guides in the library was represented after each transformation (∼37,000 colonies).20 Colonies were scraped and midi-prepped (Zyppy Plasmid Midiprep Kit, Zymo Research, Irvine, CA) in preparation to be transformed into strain W.
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted and stored at −20°C for at least six hours prior to extraction using Zymo Yeast Plasmid Miniprep II (Zymo Research # D2004), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the LentiCRISPRv2 hygro plasmid was purified using a 0.7% agarose gel and a Zymoclean gel DNA recovery kit (Zymo Research cat # D4001). The prepared sgRNA oligo duplexes were then ligated into the purified LentiCRISPRv2 hygro plasmid ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were extracted from a 10 mL culture (5 OD) of sorted cells subpopulations using the Zymoprep™ Yeast Plasmid Miniprep II (Zymo Research) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... coli cells were recovered by centrifugation and pGFP was purified using ZymoPURE™ Plasmid Maxiprep Kit (catalog #D4203, Zymo Research, Irvine, USA). The DNA concentration was measured in a NanoDrop™ 2000/2000c Spectrophotometer (ThermoScientific® ...
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bioRxiv - Genetics 2023Quote: ... The amplicons were treated with DpnI restriction enzyme to remove the plasmid template and transformed into XL10 gold competent cells prepared by ‘Mix and Go!’ transformation kit (Zymo Research, T3001). The sequence of the isolated plasmids was confirmed using nanopore sequencing (Plasmidsaurus ...
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bioRxiv - Bioengineering 2023Quote: Wild type 4D5 and 5A12 VH and VL scFv gene fragments were purchased from Twist Bioscience and transformed into the BamHI digested pYD1 plasmid via homologous recombination (Boder and Wittrup, 1997; Chao et al., 2006) using Frozen-EZ Yeast Transformation II (Zymo Research, T2001). 4D5 scFv was transformed in a HA-VL-218-VH-FLAG orientation and 5A12 scFv as HA-VH-218-VL-FLAG ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from LV apical core tissue and from beating human iPS cell derived cardiomyocytes using Quick-RNA Miniprep Plus (Zymo Research, Irvine, CA, USA). The qRT-PCR was performed using SYBR mix (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Biochemistry 2021Quote: ... which were then grown in liquid culture prior to purification of the plasmid library using a ZymoPure II Endotoxin-Free Midiprep kit (Zymo Research, Irvine, CA). An analysis of our final DMS data suggest this library contains at least 7,614 of 7,686 possible variants ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmid DNAs were linearized by restriction enzyme digest (SpeI) and purified using the Zymo DNA Clean and Concentrator protocol (Zymo Research, Irvine, CA). In vitro transcription of mRNA was performed using the T7 mScript Standard mRNA Production System (Cellscript ...
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bioRxiv - Synthetic Biology 2020Quote: ... MCs were further cleaned to remove any PP contamination using Plasmid-Safe ATP Dependent DNase kit (Epicenter, WI, USA) followed by the DNA Clean & Concentrator Kit (Zymo Research, CA, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... High yield pure plasmid DNA preparations were subsequently obtained from 100 mL overnight LB broth cultures using the endo-free ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, USA).