Labshake search
Citations for Zymo Research :
101 - 150 of 487 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2024Quote: ... Plasmids were isolated from the remaining culture using the ZR Plasmid Miniprep – Classic kit (Zymo).
-
bioRxiv - Biophysics 2020Quote: ... All the plasmids were amplified using ZymoPURE II Plasmid Midiprep Kit (D4201; Zymo Research, California, USA) and their concentration and purity were confirmed by a UV-Vis spectrophotometer (NanoDrop 2000 ...
-
bioRxiv - Genetics 2021Quote: ... We extracted plasmid from clones or pools of clones using the Yeast Plasmid MiniPrep Kit (Zymo) and transformed this into bacteria for amplification.
-
bioRxiv - Genetics 2020Quote: ... and plasmid DNA was harvested using the ZymoPure Plasmid Midiprep Kit (ZYMO Research, Irvine, California, USA). After sequence confirmation ...
-
bioRxiv - Genetics 2021Quote: Plasmids were extracted from the yeast transformant pool using Zymoprep Yeast Plasmid Miniprep II (Zymo Research). Plasmid fragments containing the barcode and variant were isolated using M13/pUC primers with KAPA HiFi Hotstart Readymix and the following cycling conditions ...
-
Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... Endotoxin-free plasmid library DNA was prepared using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research). To clone the library of gRNA pairs ...
-
bioRxiv - Developmental Biology 2022Quote: ... Plasmid DNA for each verified construct was isolated using ZymoPURE II Plasmid Midiprep kit (Zymo Research).
-
bioRxiv - Immunology 2020Quote: ... and the plasmids were purified using ZymoPURE™ Plasmid Miniprep Kit (Zymo Research, Irvine, CA, USA). The sgRNA sequence in each plasmid was validated by Sanger sequencing according to the method described above (see Supplementary Table 3 for list of sequencing primers).
-
bioRxiv - Genetics 2019Quote: ... pBID-UASC-FG-Ras plasmids were prepared with a ZymoPURE II Plasmid Midiprep Kit (Zymo Research) and sent to Model System Injections (Durham ...
-
bioRxiv - Synthetic Biology 2022Quote: ... plasmids were extracted from 1 ml of culture using the ZymoPURE Plasmid Miniprep Kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: The plasmids from sorted libraries were extracted using Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). The xylanase inserts were amplified by PCR ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasmid libraries were isolated from cells pellets using ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, D4203) per manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... The injection mix was prepared by mixing the purified plasmid DNA (Zymo D4016 PLASMID MINIPREP-CLASSIC) with DNA lad-der (1 kb Plus DNA Ladder ...
-
bioRxiv - Bioengineering 2023Quote: ... Colonies were collected and the plasmids were extracted with Zymoprep Yeast Plasmid Miniprep II (Zymo Research). For the next rounds of mutagenesis ...
-
bioRxiv - Microbiology 2023Quote: Plasmids were retrieved from yeast using the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research: D2004). The protocol was modified according to Starr et al. ...
-
bioRxiv - Genomics 2023Quote: ... and plasmid DNA was extracted by using the Zymopure II plasmid midiprep kit (Zymo research #D4201).
-
bioRxiv - Genomics 2023Quote: ... and plasmid DNA was extracted by using the Zymopure II plasmid midiprep kit (Zymo research #D4201). Transfection of plasmid DNA (2.5μg vector + 1μg of transposase plasmid ...
-
bioRxiv - Genomics 2024Quote: ... The plasmid pool was extracted and purified using ZymoPURE II Plasmid Maxiprep Kit (#D4202, Zymo Research).
-
bioRxiv - Cell Biology 2024Quote: ... and plasmid DNA was prepared using the ZymoPURE II Plasmid DNA Maxiprep kit (Zymo Research D4202). Three electroporations were mixed proportionally to their electroporation efficiency for a combined total library coverage of ∼27-fold ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were isolated by miniprep (Zymo) and a 243 bp region of the ori was amplified (pBR322-5prime-promoter1-rev 5’-TCTACACTCT TTCCCTA-CAC GACGCTCTTC CGaTcTCAGA CCCCGTA-GAA AAGaTcAAAG GaTcTTC-3’ ...
-
bioRxiv - Genomics 2021Quote: ... prior to plasmid purification (Zymo, D4209).
-
bioRxiv - Biochemistry 2023Quote: ... Yeast plasmid miniprep II (Zymo Research) was used for plasmid preparation from yeast ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Plasmid DNA was purified with the ZR Plasmid Miniprep or ZymoPure II Midiprep kit (both Zymo Research). The integrity of all plasmids was verified by Sanger Sequencing (GENEWIZ Europe).
-
bioRxiv - Systems Biology 2022Quote: ... and plasmid DNA was extracted with multiple ZymoPURE II Plasmid Midiprep columns (Zymo Research cat. no. D4200). Library coverage was estimated by counting the number of bacterial colonies on a 1:1,000 dilution plate and a 1:10,000 dilution plate ...
-
bioRxiv - Genomics 2020Quote: ... Endo-free plasmids used for mammalian transfection were prepped using ZymoPURE II Plasmid Midiprep (Zymo Research Corporation) from 50 mL Mach1 (ThermoFisher ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Plasmid purification was carried out using the The ZR Plasmid MiniprepTM-Classic (Zymo Research, Irvine, CA, USA) and sequenced by Macrogen Europe (Amsterdam ...
-
bioRxiv - Synthetic Biology 2019Quote: ... All of the plasmid DNA was extracted with a Zyppy – 96 Plasmid Miniprep kit (Zymo, Irvine, CA) following the next steps ...
-
bioRxiv - Biochemistry 2022Quote: ... Plasmids were isolated from individual bacterial colonies using a ZR plasmid miniprep kit (Zymo Research cat # D4054). The resultant plasmids were screened with restriction enzyme-digest and agarose gel electrophoresis ...
-
bioRxiv - Bioengineering 2022Quote: ... Final plasmids were maxipreped using the ZymoPURE II Plasmid Maxiprep kit (Zymo Research, Cat No./ID: D4202) and sanger sequenced in preparation for embryonic microinjection ...
-
bioRxiv - Biochemistry 2022Quote: ... Plasmids were isolated from individual bacterial colonies using a ZR plasmid miniprep kit (Zymo Research cat # D4054). The resultant plasmids were screened with restriction enzyme-digest and agarose gel electrophoresis ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and plasmid DNA was extracted using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research, Irvine, CA, #D4201).
-
bioRxiv - Immunology 2022Quote: ... Plasmids were isolated from expanded bacterial colonies using the ZymoPURE™ II Plasmid Kit (Zymo Research #D4203). Ten μg of isolated plasmid was transfected into HEK293T cells using Lipofectamine® 2000 Transfection Reagent (Invitrogen #11668-019) ...
-
bioRxiv - Genomics 2022Quote: ... plasmid DNA was harvested from the 1L culture using the ZymoPURE II Plasmid Gigaprep (Zymo Research, #D4204). Before prepping ...
-
bioRxiv - Plant Biology 2022Quote: ... Plasmid DNA was prepared from confirmed clones using the Zyppy Plasmid Miniprep Kit (Zymo Research, Irvine, CA).
-
bioRxiv - Molecular Biology 2023Quote: Plasmids from the yeast cells were extracted using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... Plasmids from these strains were isolated using a ZymoPURE plasmid miniprep kit (Zymo Research Corporation, Irvine, CA) and transformed into BW25113 using a Mix and Go Transformation kit (Zymo Research ...
-
bioRxiv - Cell Biology 2020Quote: ... the plasmid DNA was purified using the ZR Plasmid Miniprep™ Classic Kit (Zymo Research; Irvine, CA, USA) and sequenced (Eurofins MWG Operon GmbH Ebersberg ...
-
bioRxiv - Genetics 2020Quote: ... ACE2 mutant library yeast display plasmids were rescued using the ZymoPrep Yeast Plasmid Miniprep II kit (Zymo Research). Rescued plasmids were amplified via electroporation into 10G Supreme E ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Plasmid preparations were made using the Zippy Plasmid Mini Prep Kit (Zymo Research Ltd., Orange County, CA, USA). Horizontal electrophoresis of DNA was carried out using 1% Seakem LE agarose gels (Lonza ...
-
bioRxiv - Immunology 2021Quote: ... Plasmids containing TCR libraries were purified using a ZymoPURE II Plasmid Maxiprep Kit (D4202, Zymo Research, Irvine, CA). The TRBC-p2A-TRAL insert was amplified using a KAPA HiFi HotStart PCR Kit (7958897001 ...
-
bioRxiv - Molecular Biology 2019Quote: Plasmids from the final round of selection were isolated using the Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research) and sequenced ...
-
bioRxiv - Genomics 2021Quote: ... Plasmids were extracted from sorted cell pellets using the Zymoprep Yeast Plasmid Miniprep I kit (Zymo Research D2001). Each sorted sample was split into two for PCR replicates ...
-
CRISpy-pop: a web tool for designing CRISPR/Cas9-driven genetic modifications in diverse populationsbioRxiv - Genetics 2020Quote: ... The plasmids with the inserted sgRNAs were each isolated using the ZR Plasmid Miniprep Classic kit (Zymo Research). We confirmed correct sgRNA sequence insertion by performing BigDye™ (Applied Biosystems ...
-
bioRxiv - Zoology 2023Quote: ... high titer plasmid DNA was isolated using a ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, Irvine, USA) and used for transfection of mammalian cells utilized in the functional heterologous receptor assay.
-
bioRxiv - Microbiology 2024Quote: ... Transformed Escherichia coli colonies with successful insert were plasmid extracted using the Zyppy Plasmid Miniprep kit (Zymo Research). Plasmid copy numbers were estimated using the formula:
-
bioRxiv - Microbiology 2019Quote: ... The Zyppy Plasmid Miniprep Kit (Zymo Research) was used to purify plasmid DNA ...
-
bioRxiv - Bioengineering 2023Quote: ... Final selected plasmids were maxi-prepped (Zymo Research ...
-
bioRxiv - Microbiology 2022Quote: The ZymoBIOMICS human fecal reference sample (Zymo D6323) was thawed at room temperature and 100 μL was taken and centrifuged at 35g for 20 minutes to separate cells from large fecal particles ...
-
bioRxiv - Genomics 2019Quote: ... we estimated the complexity as >500K and extracted the plasmid using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research).
-
bioRxiv - Genomics 2021Quote: ... and plasmids from both before and after selection were isolated using the Yeast Plasmid Miniprep II Kit (Zymo Research).