Labshake search
Citations for Zymo Research :
51 - 100 of 143 citations for Hepatitis C Virus Core Antigen HCcAg since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The elution was incubated at 65°C over-night and DNA was extracted with DNA purification kit (Zymo D4013). Library was sequenced on the Illumina HiSeq X instrument to 400 million 150 bp paired end reads.
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bioRxiv - Cell Biology 2021Quote: ... Samples were stored at −20°C until RNA was isolated using the Direct-zolTM-96 RNA plate isolation (Zymo), 5PRIME Phase Lock Gel extraction (Quantabio ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were either resuspended in 30μL 100% RNALater and stored at -70°C or in 100μL TRI reagent (Zymo Research). Samples were crushed with a micropestle and RNA was extracted using a Direct-zol RNA MicroPrep kit (Zymo Research) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054), following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... for at least 1.5 hr at 56°C and purified using ChIP DNA Clean and Concentrator Kit (Zymo Research).
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bioRxiv - Physiology 2021Quote: ... molecular samples were digested at 55°C in 300 µl of DNA/RNA Shield (Zymo Research, Irvine, CA, USA) for two to three-and-a-half hours and centrifuged at 2200 RCF for one minute to separate the remaining solids ...
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bioRxiv - Immunology 2021Quote: ... by incubation for 45 minutes at 37°C and immediate purification using Zymo DNA Clean & Concentrator kit (Zymo D4005). Shortly before transformation ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were incubated (37°C, 30 min) and purified with the Zymo RNA Clean & Concentrator Kit (Zymo Research). The concentration of purified RNA was determined (Qubit™ RNA HS Assay Kit) ...
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bioRxiv - Genomics 2023Quote: ... and stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054) following manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... C shaker for 12h-16h prior to harvesting for plasmid purification using ZymoPURE II Plasmid Midiprep kit (Zymo D4200). Based on the colony forming units from the small volumes in the bacterial plates ...
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bioRxiv - Molecular Biology 2024Quote: Liver samples were collected at euthanasia and stored at -80°C in 1X DNA/RNA Shield™ from Zymo Research ...
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bioRxiv - Developmental Biology 2020Quote: ... and stored in −80°C until extraction of total RNA extraction using the Direct-zol™ RNA kit (Zymo Research). The optical density value of total RNA was measured using the NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... The reaction was incubated 10 minutes at 55°C followed by purification with DNA Clean & Concentrator-5 kit (Zymo Research) and elution in 21 µL of water ...
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bioRxiv - Bioengineering 2020Quote: ... at 37°C for 1 hour to remove the gblock template then purified using a RNA spin column (Zymo Research). Purified RNA was then treated with 200 units of calf intestinal phosphatase (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
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bioRxiv - Immunology 2021Quote: ... Samples were stored in RNAlater (Invtrogen) for 24 hours at 4°C and then homogenized in TRI Reagent (Zymo Research) with a beadbeater ...
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bioRxiv - Immunology 2021Quote: ... Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo). Fragments were amplified according to the previously published protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: 208 female beetles (Table 1) p stored at −80°C were homogenized in Tri-Reagent® (Zymo Research, California, USA) using an electric bead mill ...
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bioRxiv - Genomics 2020Quote: ... was added and incubated for 60 min at 50 °C and the DNA was purified using the Oligo Clean & Concentrator kit (Zymo). DNA was analyzed for oxidized 5mC modifications using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated for 16 hours at 16 °C and were terminated by cleaning with RNA Clean & Concentrator (Zymo Research). Crosslink reversal was done by irradiating the RNA on ice with 2.5 KJ/m2 254 nm UVC using a CL-1000 crosslinker (UVP).
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... Crosslinks were reversed at 65°C overnight and DNA was purified with ChIP DNA Clean & Concentrator kit (Zymo Research, D5205). Sequencing libraries were prepared using the KAPA Hyper Prep kit ...
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bioRxiv - Molecular Biology 2023Quote: ... for 2 hours at 25°C after which the circularized RNA was purified with the Oligo Clean & Concentrator kit (D4061) by Zymo Research ...
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bioRxiv - Genomics 2023Quote: ... at 37 °C for 2 hours and purified using Zymo ssDNA/RNA clean & concentrator kit following manufacturer’s instructions (Zymo D7011). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... and the 100–400nt region was cut and dissolved in 1X TEN250 buffer (1X TE 7.4, 0.25M NaCl) at 80°C for 30min followed by column purification (Zymo research, R1016). For the HEK293T libraries ...
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bioRxiv - Microbiology 2023Quote: RNA was isolated from mid-log phase protists grown at normal growth temperature or 12°C using the Direct-zol RNA Miniprep kit (Zymo). RNA libraries were generated using the KAPA Stranded mRNA Sequencing Kit (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Developmental Biology 2024Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were treated with 1 μL of DPNI for 3 h at 37°C and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s protocol (Zymo CN# D4004).
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bioRxiv - Genomics 2020Quote: ... The 300 bp sized Micro-C library was purified via 1.5 % agarose gel electrophoresis and extracted with Zymoclean Gel DNA Recovery Kit (Zymo Research, D4002) with a final elution volume of 50 μL ...
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bioRxiv - Developmental Biology 2021Quote: ... The reactions were incubated for 30 min at 37°C and immediately purified using ZYMO RNA Clean & Concentrator (ZYMO RESEARCH, D7011). The purified RNA samples were eluted in 20 μl of nuclease free water ...
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bioRxiv - Biochemistry 2022Quote: ... These sorted libraries were grown for 1 day at 30°C shaking in SD-CAA media and then 300 µl of the cultures were miniprepped (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... purified PCR product was digested with EcoRI-HF and BamHI-HF at 37°C for 1.5 hours and purified again using a Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). For the cloning backbone ...
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bioRxiv - Molecular Biology 2021Quote: ... The reactions were treated with DNAse (15 min at 37°C) and remaining RNA was recovered using Direct-zol RNA mini prep kit (Zymo research) according to the protocol ...
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bioRxiv - Microbiology 2022Quote: ... and stored at -20°C until (≤ 3 days) the DNA was extracted via the Zymo Quick-DNA Fecal/Soil Microbe kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Prepared lysates were stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Digests were heat inactivated for 20 minutes at 80°C then column cleaned using the DNA Clean and Concentrator kit (Zymo Research). Dilute ligations (100-500 ng DNA ...
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bioRxiv - Biochemistry 2019Quote: ... Beads for protein analysis were incubated with Laemmli buffer and heated at 95°C for 10 min and beads for RNA analysis were exposed to TRI reagent (Zymo Research) for RNA extraction using Direct-Zol (Zymo Research ...
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bioRxiv - Molecular Biology 2019Quote: ... the DNA was treated with sodium bisulfite under non-denaturing conditions (no denaturation and incubation at 37°C for 3 hrs) using the Zymo Lightning bisulfite modification kit (Zymo Research). Following sample clean-up ...
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bioRxiv - Biochemistry 2019Quote: ... the DNA samples used for 450k analysis were digested overnight at 37°C using BamHI and EcoRI and then bisulfite converted using EZ DNA Methylation-Lightning Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The intermediate RNA products were removed with NaOH-EDTA solution in 95 °C for 10 minutes and the single strand DNA oligo probes were purified via DNA clean & concentrator-25 (D4033, Zymo Research). The ssDNA probes were either directly used for Oligopaint FISH or store in aliquots at −80°C.
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bioRxiv - Cancer Biology 2020Quote: ... Transposition was carried out at 37°C for 30 minutes followed by DNA purification with DNA Clean and Concentrator-25 (Zymo Research) according to manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2019Quote: ... Elutions were subsequently de-crosslinked overnight at 65 °C and DNA was purified using ChIP DNA Clean and Concentrator (Zymo Research) and DNA was used for qPCR ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 300µm diameter) follwing a Proteinase K digestion (at 55°C for 2 hours) using a Quick-RNA MicroPrep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
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bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged 1 min at 12000 g at 4°C and RNAs were extracted from supernatants with Direct-zol RNA Mini Prep kit (Zymo Research), following manufacturer’s protocol and including the optional DNAse treatment step ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were incubated for 1 hour at 37 °C and DNA was purified using the DNA Clean & Concentrator-25 kit (Zymo Research) as described by the manufacturer.
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bioRxiv - Genomics 2021Quote: ... The mixture was incubated at 37°C for 30 minutes and subsequently purified using the ChIP DNA purification kit (Zymo Research) as described by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... The extracted RNA was treated with DNase I for 15 minutes at 37°C and further purified using a Quick-RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).