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Citations for Zymo Research :
101 - 150 of 159 citations for FABP2 I FABP Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted with Direct-zol RNA miniprep kit with on-column DNase I digestion (Zymo Research #R2053). 0.5 to 1 μg of total RNA was used for reverse-transcription with ReverTra Ace qPCR RT master mix (Toyobo #FSQ-101 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA was treated with DNase I and cleaned using an RNA Clean and Concentrator Kit (Zymo Research, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: RNA from human RPTECs was extracted using the Direct-zol MicroPrep Plus Kit (Zymo) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cultured or purified human cells were lysed directly in RNA lysis buffer (Zymo Research) and homogenized by pipetting ...
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bioRxiv - Plant Biology 2021Quote: ... RNA samples were digested with DNase I and purified using RNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from seedlings using a Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research). cDNA synthesis was performed with 2 µg of total RNA using oligo(dT ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted using the Quick RNA Fungal/Bacterial Miniprep kit including DNase I column treatment (cat#R2014, Zymo Research). RNA was eluted in 30μL of molecular grade water and assessed for concentration and quality via Nanodrop and RNA gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... eluted RNA is spun out into fresh tubes and a second elution with water is performed (Zymo-I = 30μL, Zymo-II = 50μL, Zymo-IIICG = 60μL). To the eluate ...
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bioRxiv - Microbiology 2023Quote: ... The cells debris was removed after centrifugation at 15000g for 5 mins and cell supernatant containing the released virions were subjected to 5μl DNase I (Zymo research) for 1 hour to remove remaining untransfected chimeric NL plasmid ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed and corrected recombinant plasmids were digested with Not I for linearization and then transformed into Po1fΔKu70 using the Frozen-EZ yeast transformation II Kit (Zymo Research). Transformants were screened on YNB plates supplemented with auxotrophic supplements containing appropriate amino acids and placed in an incubator at 30 °C for 2-3 day ...
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bioRxiv - Cell Biology 2024Quote: ... when RNA was further purified using the RNA Clean & Concentrator kit with in-column DNase-I treatment (Zymo Research Corp), following manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from primary human PTC with the Direct-zol MicroPrep Kit (Zymo) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and the RNA was extracted using the Direct-zol™ RNA purification kit following the manufacturer’s protocol for DNase I digestion (Zymo Research). cDNA was synthesized with Maxima Reverse Transcriptase (Thermo Scientific™ ...
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bioRxiv - Genetics 2019Quote: ... Total RNA was isolated from cell pellets in 5 batches (each batch contained one of each of the 5 strains) using the ZR Fungal/Bacterial RNA mini-prep kit including the DNase I on-column digestion step (Zymo Research). Each cell pellet was resuspended in lysis buffer and transferred to screw-capped tubes containing glass beads and the cells were broken open using a mini-beadbeater (Biospec Products ...
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bioRxiv - Microbiology 2021Quote: ... Exceptions to these protocols were as follows: (i) RNA purification after acylation was performed using RNA C&C columns (Zymo Research), rather than ethanol precipitation ...
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bioRxiv - Genomics 2020Quote: ... The reaction was then treated with DNase I to remove template DNA and RNA product was purified using an RNA Clean and Concentrator-5 (Zymo #R1016). Purified RNA was used as the template for reverse transcription using ProtoScript II according to manufacturer protocol ...
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bioRxiv - Cell Biology 2022Quote: ... An aliquot of the supernatant was then used for RNA purification with on-column DNase-I treatment (Direct-Zol RNA Microprep R2060, Zymo Research). RNA concentration and purity was determined by spectrophotometry (NanoDrop 1000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... After the second treatment with DNase I the samples were column purified and concentrated using RNA Clean & Concentrator-5 kit (Zymo Research). Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... and total RNA was extracted from the tissue powder using a Direct-Zol RNA MiniPrep kit with DNase I treatment (Zymo Research) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... and Direct-zol RNA Miniprep Plus kits followed by on-column DNase I treatment for the removal of contaminating DNA (Zymo Research). To measure PIV5-W3 transcription ...
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bioRxiv - Cell Biology 2021Quote: ... human islets or EndoC-βH5 cells using a commercial column-based purification with DNAse I treatment (Direct-Zol RNA MicroPrep with Trizol Reagent, Zymo Research). RNA was quantified by Nanodrop spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The extracted RNA was treated with DNase I for 15 minutes at 37°C and further purified using a Quick-RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2022Quote: ... The samples were treated with DNase I and cleaned using ethanol precipitation and subsequent RNA Clean & Concentrator-5 spin-column purification (Zymo Research). Successful removal of genomic DNA was confirmed by PCR using a host-specific primer pair (Supplementary Figure S1) ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from cell lysates using the Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research, USA) according to the mnufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: The 2 μL of EVs were incubated at room temperature for 15 min with 1 U of DNase I (Zymo Research) and 1x DNA Digestion Buffer ...
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bioRxiv - Molecular Biology 2023Quote: Total genomic DNA was extracted from ∼0.2 mg (i) spinach and (ii) mixed-species Teklad samples using a Zymo Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research). Following extractions ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from whole embryos or isolated tailbuds prepared as described above was treated with RNase-free DNase I (Zymo Research) to remove genomic DNA ...
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bioRxiv - Bioengineering 2024Quote: The 10 μL of eEVs were incubated at room temperature for 15 min with 1 U of DNase I (Zymo Research) and DNA Digestion Buffer ...
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bioRxiv - Genomics 2022Quote: RNA from human RPTECs was extracted with the TRIZOL and Direct-zol MicroPrep Plus Kit (Zymo) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed in TRIzol buffer and RNA was isolated using RNA Clean Concentrate Kit with in-column DNase-I treatment (Zymo Research Corp), following manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... and RRBS Libraries were prepared from 40 ng of genomic DNA digested with Msp I and then extracted with DNA Clean & Concentrator™-5 kit (Zymo Research). Fragments were ligated to pre-annealed adapters containing 5’-methyl-cytosine instead of cytosine according to Illumina’s specified guidelines (www.illumina.com) ...
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bioRxiv - Synthetic Biology 2021Quote: Plasmids were isolated from 5-10 mL yeast cultures either by alkaline lysis (39) or using Zymo Yeast Miniprep Kit I (Zymo Research D2001). Plasmids were then recovered into DH10B ElectroMax E ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from three biological replicates of E/I networks composed of Ngn2 #2 and Ascl1 #1 (DIV 49) with the Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA was isolated using TRIzol and the Direct-zol™ RNA MiniPrep Kit with two times of in-column DNase I treatment according to the manufacturer’s instructions (Zymo Research, R2053). RNA purity and concentration were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: RNA isolation of digested samples was performed using the Directzol RNA Miniprep kit with DNAse-I treatment to remove trace DNA before RNA elution (Zymo Research, R2050). RNA was quantified via Nanodrop spectrophotometry ...
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bioRxiv - Genetics 2020Quote: ... DNA was digested in columns by adding a mixture of 5 μl RNA Lysis Buffer 1 from the kit andl DNase I and 75 μl RNA Lysis Buffer 1 from the kit andl DNA Digestion Buffer from DNase I Set kit (Zymo Research E1010) followed by a 15-minute incubation at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was purified from the aqueous phase using the RNA Clean & Concentrator-5 kit with DNase I treatment to remove genomic DNA contamination (Zymo Research R1013). RNA quantity was determined with the Qubit RNA Broad Range Assay (ThermoFisher Scientific Q10210) ...
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bioRxiv - Molecular Biology 2023Quote: ... 38% v/v saturated acidic phenol) and the Direct-zol RNA MiniPrep Kit (including on-column DNase I treatment, Zymo Research, R2052) or according to the protocol for TRIzol™ Reagent (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... The transcribed product was treated with DNase I for 30 min at 37°C and then purified using RNA Clean and Concentrator Kit (Zymo Research #R1016).
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bioRxiv - Plant Biology 2024Quote: Total RNA from seedlings was isolated using a Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research, Irvine, CA). cDNA was synthesized using Oligo(dT ...
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bioRxiv - Genomics 2023Quote: ... extracted RNA was concentrated and treated with DNAse I utilizing on-column digestion following the manufacturer’s protocol (Zymo Research, Irvine, CA, USA). The resulting RNA was depleted of ribosomal RNA for two hours ...
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bioRxiv - Cancer Biology 2024Quote: RNA from K562 and Nalm6 cells was isolated using the NucleoSpin RNA Mini kit (Macherey Nagel) followed by DNase-digestion with the DNase I set (Zymo Research, #E1010) and clean-up using the NucleoSpin RNA Clean-up Mini Kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from 50-100 mg seedlings using the Quick-RNA Miniprep Kit with on-column DNase I digestion (Zymo Research, Irvine, CA). cDNA was synthesized with 2–2.5 μg total RNA using the Invitrogen SuperScript III Reverse Transcriptase and the Oligo(dT)20 primer (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast cells transformed with plasmids encoding the CDM library or control variant are harvested and the plasmid DNA extracted using the Zymoprep Yeast Plasmid Miniprep I Kit (Zymo Research, Irvine, CA). DNA sequences of the variant library are then PCR-amplified using primers with NGS adapters and gel-extracted from the reaction mixture by length of the PCR product ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNase I treated RNA was further purified into small (<200nt) and large (>200nt) RNA fractions using RNA Clean & Concentrator™-25 (Zymo Research # R1017) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Digestion was confirmed with methylated and unmethylated human DNA standards provided with the OneStep qMethyl kit (Zymo) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Bisulfite conversion was confirmed by PCR using Universal Methylated Human DNA Standard with hMLH1 Primers (Zymo Research). Converted DNA was then applied to an Illumina Infinium MethylationEPIC850 BeadChip (865918 probes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... total RNA was extracted and genomic DNA was removed using the Direct-Zol™ Miniprep Kit with an in column DNase I treatment (Zymo Research, Irvine, CA). cDNA was prepared from total RNA using the iScript™ Reverse Transcription System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... eluted RNA is spun out into fresh tubes and a second elution with water is performed (Zymo-I = 30μL, Zymo-II = 50μL, Zymo-IIICG = 60μL). To the eluate ...