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Citations for Zymo Research :
651 - 700 of 946 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: RNA was isolated from the sorted CD8+ T cells using the Direct-zol RNA MiniPrep (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA of the snap frozen cells was extracted using the Quick-DNA Midiprep Plus Kit (Zymo Research, D4075). sgRNA amplification was performed following a previously published protocol(49) ...
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bioRxiv - Systems Biology 2023Quote: ... 3 μl of reaction mix were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Systems Biology 2023Quote: ... We thawed the cell pellets on ice and then resuspended with 400 µL RNA binding buffer (Zymo, #R1013-2). The mixtures were then transferred to 2 mL screw cap tubes with zirconia beads inside ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA (gDNA) was purified from ES cells and tail clips using the Quick-DNA Miniprep Kit (Zymo Research). Initial validation of target insertion was performed with primers designed to amplify across the 5’ and 3’ integration sites (Supplementary data 1-4) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from transduced HEK 293 cells using the Zymo Quick-RNA Microprep Kit (Zymo Research, #R1050). Reverse transcription was performed with Invitrogen’s SuperScript IV VILO Master Mix with ezDNAse enzyme (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... At least four million IL-1βlow and IL-1βhigh cells were collected for genomic DNA extraction using Quick-DNA FFPE Kit (Zymo Research). PCR reactions containing up to 10 μg genomic DNA in each reaction were performed using ExTaq DNA Polymerase with primers to amplify the sgRNAs ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from transduced HOS cells using a Quick-DNA Miniprep kit (Zymo Research, Irvine, CA, USA). Vector titers were determined by calculating the ratio between the copies of HIV psi and every two copies of RPP30 via QX200 digital droplet PCR system (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: RNAs from respective cell lines were isolated and purified via the Zymo Quick-RNA microprep kit (Zymo Research, #R1058). 1000 ng of DNAse-treated RNA was converted to cDNA using the iScript cDNA synthesis kit (BioRad ...
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bioRxiv - Immunology 2023Quote: ... cells and tissue were harvested in TRIzol and RNA was isolated using Direct-zol RNA Miniprep kits (Zymo Research) with 1 h DNase treatment ...
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bioRxiv - Genetics 2024Quote: RNA was extracted from total cell lysates directly from beads after IP using TRIzol RNA Miniprep Plus (Zymo Research). First ...
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bioRxiv - Immunology 2024Quote: Bisulfite modification of genomic DNA from FACS purified cells was performed using the EZ DNA methylation kit (Zymo Research). For methylation analysis on Ifng gene promoter CpG sites ...
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bioRxiv - Bioengineering 2024Quote: ... The cell samples were lysed with Trizol (Life-science) and RNA purification was done with Zymo kit (R1050, Zymo). RNA quantification was assessed with a Nano Drop 8000 Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was purified from these cell lysates using the standard protocol in the DNA extraction kits (Zymo Research #D3010), except that DNA was resuspended in sterile water in the final step ...
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bioRxiv - Immunology 2021Quote: ... containing 1% pME and total RNA was isolated using the Quick-RNA MicroPrep Kit (Zymo Research, 11-328M). All subsequent RNA was used for reverse transcription using random hexamer and oligo dT primers with SuperScriptll (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... An aliquot of each lung homogenate (300µL) was added to 100µL of TRIReagent (Zymo Research R2050-1-200) and stored at −80°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Fragments were amplified using overhang primers (Supplementary table 1) and purified using DNA Clean & Concentrator kit (Zymo Research). The pUC19-iTol2 backbone was digested with BamHI-HF (NEB ...
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bioRxiv - Immunology 2022Quote: ... 500μL of nasal wash was added to 500μL of TRIzol reagent (Zymo Research Catalog No R2050-1-200) and the remainder of the nasal wash was stored for serological analysis ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng - 1 μg of poly(A)+ RNA was converted using the EZ RNA methylation kit (Zymo Research) with a modified high-stringency conversion condition (Sulfonation ...
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Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... 1) Amplification of a dilution series of a ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA U.S.A.), 2 ...
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bioRxiv - Neuroscience 2022Quote: ... linear PCR products were purified from agarose gel (1%) with a Zymoclean-Gel DNA Recovery kit (Zymo Research) and circularized with DNA Ligation Kit ‘Mighty Mix’ (Takara) ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour at room temperature followed by isolation with a RNA Clean & Concentrator-25 kit (Zymo Research). 1 µg RNA was converted to ribosomal depleted cDNA libraries ready for sequencing using the TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat kit (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng-1 μg of genomic DNA was bisulfite converted using the EZ DNA Methylation-Gold kit (Zymo). Bisulfite-converted DNA was PCR amplified ...
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bioRxiv - Cell Biology 2024Quote: ... After purifying both PCR products using a 1% agarose gel and a gel purification kit (Zymo, no. D4007), the two fragments were assembled using a Gibson assembly mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... the PCR master mix has been treated with 0.02% (v/v) of DNAse I 1 U/mL (Zymo) at room temperature for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was extracted from no treatment and SHX-treated flash frozen cells using the Quick RNA extraction kit (Zymo) following the standard protocol ...
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bioRxiv - Cell Biology 2019Quote: RNA was typically isolated from confluent monolayers of cells seeded in 10-cm plates using the direct-zol RNA miniprep kit from Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from cells lysed with Trizol using the Direct-zol RNA Miniprep Plus Kit (Zymo Research, R2072). 1 µg of total RNA was reverse transcribed using Maxima H Minus Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... One batch of B cells was then harvested immediately after infection (day 0 of infection) with TRI Reagent (Zymo Research) and the rest of the infected cells were kept in culture for 3 days at 37°C ...
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bioRxiv - Genomics 2020Quote: ... Cells were lysed in Tri-reagent and total RNA was isolated using the Direct-zol RNA mini-prep kit (Zymo). For spike-in ...
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bioRxiv - Microbiology 2019Quote: ... Transformation of yeast cells was performed according to manufacturer's guidelines using the Frozen‑EZ Yeast Transformation II Kit (Zymo Research) and cells were selected on complete supplement mixture (CSM ...
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bioRxiv - Genetics 2019Quote: ... RNA was extracted from FACS-collected cells with TRIzol and the Direct-zol RNA MiniPrep Kit (Zymo Research, Irvine, CA). RNA quality was assessed on an Agilent 2100 Bioanalyzer utilizing the Agilent RNA 6000 Pico Kit (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... DENV RNA in cell culture supernatants was isolated according to the manufacturer’s protocol for Direct-zol RNA MiniPrep Kit (Zymo Research) and assayed by qRT-PCR ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from cells using the Direct-zol RNA MiniPrep Plus kit following the manufacturer’s instructions (Zymo research) and used for cDNA reverse transcription (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2021Quote: ... cell debris was spun down and plasmid DNA was collected using the Zymo Zyppy plasmid preparation kit (Zymo Research, D4036) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was extracted from cells with Direct-zol-96 RNA Kit or Direct-zol RNA MiniPrep Kit (Zymo Research). RNA samples were subjected to qRT-PCR analysis using AgPath-ID One-Step RT-PCR Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... used in this study has a log abundance distribution of different bacterial species and fungi ranging from 108 to 102 cells (ZymoBIOMICS Microbial Community Standard II, ZYMO RESEARCH EUROPE GMBH ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated from PG3-Oc-treated cells using the Quick-RNA mini prep kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: DNA extraction: DNA was extracted from control-RNP and IFNA-targeted cells using Quick-DNATM Microprep kit (Zymo Research, # D3021), and DNA concentration was calculated on Nanodrop OneC (Thermofisher).
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bioRxiv - Cell Biology 2022Quote: ... Cells were collected by the addition of lysis buffer and RNA extracted using the Quick-RNA MiniPrep kit (Zymo Research). Next Generation Sequencing libraries were prepared using by TruSeq RNA sample preparation kit (Illumina) ...
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bioRxiv - Microbiology 2020Quote: Total RNAs were extracted from the cells indicated in the figures using Direct-zol RNA MiniPrep kit according to the manufacturer’s instructions (Zymo Research). Equal amounts of RNA samples were used for cDNA synthesis by QuantiTect reverse transcription (RT ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was prepared from cell lines and orthotopic xenografts using Direct-zol RNA Miniprep kits (Zymo Research, Irvine, CA). RNA quality was determined with the Agilent 2100 Bioanalyzer Nano Assay (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated using the PerfectPure RNA Cultured Cell kit (5 PRIME) or Quick-RNA™ MiniPrep (Zymo Research). One microgram of the total RNA was reverse-transcribed into cDNA with M-MLV (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... genomic DNA from euploid 46,XX cells was bisulfite-converted using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA), labeled and hybridized using the Infinium Methylation EPIC BeadChip Kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from infected cells in the 96-well plate using a ZR-96 Viral RNA Kit (Zymo Research) per manufacturer instructions ...
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bioRxiv - Systems Biology 2022Quote: ... we collected the cells and extracted genomic DNA with a commercial kit (Zymo Quick DNA miniprep Plus Kit, Cat#D4068).
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was purified from WT and Chr4-cl KO ES cells using the Quick-gDNA purification kit (Zymo Research) and bisulfite-converted with the NEXTflex™ Bisulfite-Seq Kit (Bio Scientific ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed once with 1x PBS and RNA was harvested by adding Trizol reagent (Zymo Research, Irvine, CA, USA). RNA was isolated using Direct-Zol RNA MiniPrep kit (Zymo Research ...
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bioRxiv - Genomics 2021Quote: Total RNA was purified from the 10 replicate cell cultures using a Direct-zol RNA Miniprep Plus kit (Zymo Research), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from different PCa cell lines using Direct-zol quick miniprep plus RNA extraction kit (Zymo Research). cDNA was synthesized from extracted RNA using RT2 First Strand Kit (Qiagen ...