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Citations for Zymo Research :
1 - 50 of 153 citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: RNA were isolated from purified CD4+ T cells using Direct-zol RNA Purification Kit (Zymo Research) and cDNA were generated using iScript Reverse Transcriptase (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: Total RNA from purified CD8+ T cells was extracted with Direct-zol RNA MiniPrep Plus (Zymo Research) following DNA removal step ...
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bioRxiv - Microbiology 2021Quote: ... Exceptions to these protocols were as follows: (i) RNA purification after acylation was performed using RNA C&C columns (Zymo Research), rather than ethanol precipitation ...
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bioRxiv - Genomics 2019Quote: Purified DNA was bisulfite converted following the T-WGBS protocol[16] with the EZ DNA methylation kit (Zymo). Oligonucleotide replacement was performed by incubating 9 μl of tagmented M-ATAC or M-ChIP purified DNA with 2 ng of phage lambda DNA as carrier ...
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bioRxiv - Biochemistry 2020Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... into the 20 μl ligation reaction for incubation with 30 minutes at 30°C and 3 minutes at 65°C to inactivate the enzymes followed by column purification (Zymo Research, R1016). Before the reverse transcription (RT) ...
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bioRxiv - Molecular Biology 2021Quote: ... C-SCCE0625), and ScriptCap 2′-O-Methyltransferase (Cellscript, C- SCMT0625) and purified by a Direct-zol RNA Microprep Kit (Zymo Research, D2060). The radiolabeled TOP RNAs (4 pmol ...
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bioRxiv - Genomics 2022Quote: ... at 37°C for 1h and purified by Zymo columns ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from sorted memory CD4 T cells using Quick-DNA Microprep kit (Zymo Research, Irvine, CA, USA). ImmunoSEQ hsTCRB sequencing kit (Adaptive Biotechnologies ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from the sorted CD8+ T cells using the Direct-zol RNA MiniPrep (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from the sorted CD8+ T cells using the Direct-zol RNA MiniPrep (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA extraction for both T-47D and PDX samples was performed using a Quick-RNA RNA Miniprep kit (Zymo Research), and RNA was measured using a Qubit fluorometer ...
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bioRxiv - Molecular Biology 2020Quote: ... and frozen at −80°C in 500 ul TRI-Reagent (Zymo). Frozen worms were thawed on ice ...
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bioRxiv - Genomics 2020Quote: ... The genomic DNA of JLat cells and CD4 T cells from individuals #3 and #4 were extracted with a Quick-DNA Miniprep Plus kit (Zymo Research #D4068) according to the product protocol ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: RNA from HEK Flp-In™ T-REx™ 293 and A549 cells was extracted using Zymo III columns (Zymo, Freiburg, Germany). Reverse transcription was performed using random hexamer primers (IDT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and eluted in 10 μL prewarmed 65°C provided elution buffer (Zymo). Six μL of each were then combined and sequenced off the U2 region via Illumina amplicon sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and eluted in 10 μL prewarmed 65°C provided elution buffer (Zymo). Six μL of each were then combined and sequenced off the U2 region by Illumina sequencing on one lane of HiSeq4000 SR50 at the QB3 Genomics Sequencing Laboratory at UC Berkeley.
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bioRxiv - Molecular Biology 2023Quote: ... for 30min at 30°C followed by column purification (Zymo research, R1016). The following reverse transcription (RT ...
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bioRxiv - Genomics 2019Quote: ... RNA was isolated from different number of murine splenic CD3 positive T cell samples and human oocyte samples by using the Direct-zol™ RNA MiniPrep Kit (ZYMO, cat. R2050). The extracted RNA samples were dissolved into 30 µl of nuclease-free water (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... Protein degradation was performed by adding Proteinase K (Zymo) and Qiagen Protease (Qiagen) ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from each of the two technical replicates of sorted CFSElow CD4 T cells using Quick-RNA Microprep kit (Zymo Research, Irvine, CA, USA). Without measuring the resulted RNA concentration ...
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bioRxiv - Immunology 2021Quote: ... Single viable CFSElow CD3+ CD8− CD4+ T cells were sorted into 96-well PCR plates containing DNA/RNA Shield (Zymo Research, Irvine, CA, USA) using FACSAria II and Diva Software (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... which were then grown overnight at 37 °C and subsequently purified (Zymo, D4200). For each step ...
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bioRxiv - Microbiology 2022Quote: ... at 30°C for 30 min and removed by RNA Clean & Concentrator (Zymo Research). Reverse transcription reactions including the ligated RNA above ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL CircLigase) for 12 hours at 60°C and subsequently purified by Zymo RNA Clean & Concentrator 5 columns (100 μL sample ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for two hours followed by agarose gel purification (Zymo Research, USA) and overnight ligation at 4°C (T4 DNA ligase ...
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bioRxiv - Cell Biology 2023Quote: ... ChIP-DNA was prepared using ZymoMag Protein A beads (Zymo Research). Input samples were also prepared using 10% of chromatin samples ...
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bioRxiv - Molecular Biology 2022Quote: ... Digest of the vector (1 h, 37 C) was purified from agarose gel (Zymo Research). We ligated both with an insert:vector ratio of 1:4 using Ligase (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... #C3040) at 30°C and purified using the Zyppy Plasmid Miniprep kit (Zymo Research, #D4036) or ZymoPURE II Plasmid Midiprep kit (Zymo Research ...
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bioRxiv - Molecular Biology 2020Quote: ... at 37 °C for 30 minutes and was cleaned using RNA Clean & Concentrator (Zymo Research).
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bioRxiv - Developmental Biology 2024Quote: ... DNA was hydrolysed for 6 h at 37°C using DNA degradase plus (Zymo Research). Mass spectrometry quantification was performed as previously described36 in the Babraham Institute mass spectrometry facility using three individual E18.5 wild-type and three individual E18.5 Tet3 knockout embryos.
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were incubated overnight at 16°C and were terminated using RNA Clean & Concentrator (Zymo Research). Crosslink reversal was done by irradiating the RNA with 2.5 KJ/m2 254 nm UVC using a CL-1000 crosslinker (UVP ...
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bioRxiv - Genomics 2020Quote: ... for 60 min at 50 °C and subsequently purified using Oligo Clean & Concentrator kit (Zymo Research) and analyzed for oxidized 5mC modifications using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS ...
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bioRxiv - Developmental Biology 2023Quote: ... 60 min at 55 °C) and cleaned using a ChIP DNA Clean & Concentrator kit (Zymo Research). The chromatin was quality-controlled (QC ...
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bioRxiv - Molecular Biology 2020Quote: ... for 1.5 h at 56°C and purified using ChIP DNA Clean and Concentrator Kit (Zymo Research).
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bioRxiv - Molecular Biology 2019Quote: ... for 1.5 hours at 56°C and purified using ChIP DNA Clean and Concentrator Kit (Zymo Research).
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bioRxiv - Cancer Biology 2021Quote: ... incubated at 37 °C for 30min and column purified using the RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C for 30 min followed by cleanup using RNA concentrate and clean kit (ZYMO lnc.). For Northern blot analysis of HCV genomic RNA and vcircRNA ...
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bioRxiv - Developmental Biology 2021Quote: ... a mouse 110 kb BAC clone encoding Nctc1 and Igf2 was purchased from Thermo Fisher Scientific (RPCI23.C) and the DNA was prepared with a ZR BAC DNA Miniprep kit (Zymo Research (D4048)) as control ...
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bioRxiv - Molecular Biology 2019Quote: ... for 2 hours at 45°C and purified using the ChIP DNA Clean and Concentrator Kit (Zymo Research).
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bioRxiv - Plant Biology 2020Quote: ... hen2-2 se-2 plants grown at 20 °C using the Direct-zol™ RNA kit (Zymo Research). RNA was quantified using the Qubit RNA Assay Kit (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... followed by max speed centrifugation at 4 °C for 5 minutes and DNA purification by column purification (Zymo) prior to sample preparation for next-generation sequencing.
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bioRxiv - Developmental Biology 2024Quote: ... at 37°C for 30 minutes and treated with the RNA Clean and Concentrator-5 kit (Zymo Research). The purity and concentration of the resulting RNA were assessed using Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... at 37°C for 30 minutes and treated with the RNA Clean and Concentrator-5 kit (Zymo Research). A total of 1 µg of RNA was used as a template for cDNA synthesis using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT ...
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bioRxiv - Molecular Biology 2023Quote: ... for 30 min at 37 °C and column-purified using the RNA Clean & Concentrator-5 kit (Zymo Research), eluting in 12.5 μL RNase-free water ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 30 minutes at 37 °C and products were further cleaned with RNA clean & concentrator 5 (Zymo Research) and eluted with 8-10 µl RNase free water ...
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bioRxiv - Genetics 2023Quote: ... and stored in the -80°C freezer prior to total RNA prep (DirectZol RNA microprep kit, Zymo R2060). Three independent biological replicate samples were prepared for N2 males and N2 hermaphrodites ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated at 65 °C for 6 hours and extracted with ChIP DNA Clean&Concentrator kit (Zymo Research, USA).
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bioRxiv - Genomics 2023Quote: ... worms were thawed at 4°C and subsequently preserved in DNA/RNA Shield (Zymo Research, Irvine, CA, USA). Adults of T ...