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Citations for Zymo Research :
1 - 50 of 1000 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... the 50 µL aliquot plus 5 µL of 10% (w/v) SDS was purified using the Zymo RNA clean and concentrator kit (Zymo Research; R1017). The total RNA was rRNA-depleted with the Ribo-Zero plant leaf kit (Illumina ...
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bioRxiv - Genomics 2024Quote: ... 80 μM alpha-factor (Zymo, Y1001) was added to the culture for a total of 2.5 h ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5 Alpha Competent Cells (Zymo Research) per the manufacturer’s instructions and isolated using a QIAprep Spin Miniprep Kit (QIAGEN) ...
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bioRxiv - Immunology 2021Quote: ... followed by a final clean-up step by agarose-gel extraction (2% (w/v) gel) with Zymoclean Gel DNA Recovery kit (Zymo Research D4001). Yeast scFv display libraries were generated using the amplified VH and VL DNA libraries from above and the pYD1 yeast surface display vector (Addgene plasmid #73447 ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 % ethanol was added to the aqueous phase (1:1, v/v) and transferred to RNA Clean and Concentrator (RCC) ™ −5 kit (Zymo Research; R1013) and centrifuged at 16,000 x g for 30 s ...
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bioRxiv - Bioengineering 2024Quote: ... and bacterial transformations (Mix & Go! Competent Cells - DH5 Alpha, Zymo). DNA constructs were sequence confirmed by Sanger Sequencing (Elim Bio ...
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bioRxiv - Developmental Biology 2021Quote: ... and submerged in digest solution containing 1X Digest Buffer (Zymo Research, Cat#: D3050-1-5) and supplemented with 1mg/mL Proteinase K (Zymo Research ...
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bioRxiv - Genomics 2022Quote: ... were synchronized in either G1 with alpha-factor (Zymo Research, CAT. Y1001), or in late anaphase ...
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bioRxiv - Cell Biology 2024Quote: ... High salt solutions were transferred to Zymo RNA columns (Zymo RNA Clean & Concentrator 5 kit, R1013) for DNAse treatment and purification as per the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Precipitated RNA solutions were transferred to Zymo RNA columns (Zymo RNA Clean & Concentrator 5 kit, R1013) for DNAse treatment and purification as per manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... Calculations of alpha and beta diversity were done by standard methodology by Zymo Research.
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bioRxiv - Immunology 2024Quote: ... DNA fragments were purified from enzyme solution using Zymo DNA Clean and Concentrator TM -5 kit (Zymo). Libraries were barcoded (Nextera Index Kit ...
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bioRxiv - Systems Biology 2023Quote: ... We ran this concentrated library on a 2% agarose 1X TAE gel with an EtBr stain at 90 V for 85 minutes and extracted the resulting 636 bp band using a DNA Clean & Concentrator-5 Kit (Zymo, #D4014) with a 20 μL MB H2O elution ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from 1 × 106 A549 cells treated with 0.5 % (v/v) DMSO or 5 µM FICD inhibitors (C22 or C73) for approximately 24 hours using an RNA miniprep kit (Zymo Research) and quantified using a NanoDrop™ One Microvolume UV-Vis Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: G1 arrest was performed using 50 ng/ml of alpha-factor (Zymo Research, Y1001M) in Δbar1 deleted strains during 3 hours prior to imaging ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were propagated by transforming Escherichia Coli (Mix&Go! Competent Cells - DH5 Alpha, Zymo Research) using standard procedures ...
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bioRxiv - Genetics 2024Quote: ... Purified ligations were transformed into chemically competent cells (Mix & Go! Competent Cells - DH5 Alpha, Zymo Research). Sequences were confirmed by whole plasmid next-generation sequencing (Plasmidsaurus ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The sample was then centrifuged at 2300 rpm for 5 min and the supernatant was collected and concentrated and purified using a Zymo-Spin V reservoir (Zymo Research Irvine, CA, USA) and Qiagen MinElute spin column (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Most of the supernatant was removed and 750 μl of ZymoBIOMICS Lysis Solution and 19 μl of proteinase K (D3001-2-20/D3001-2-5, Zymo Research) were added to the remaining 200 μl of the samples and incubated for 30 min at +55 °C ...
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bioRxiv - Microbiology 2024Quote: ... the PCR master mix has been treated with 0.02% (v/v) of DNAse I 1 U/mL (Zymo) at room temperature for 15 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... 3 μl of reaction mix were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Bioengineering 2024Quote: ... The RNA was mixed with ethanol absolute at a 1:1 v/v ratio and submitted to a spin column-based purification protocol (Direct-zol RNA Miniprep kit, Zymo Research), including a DNAse treatment step ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then arrested in G1 using a final concentration of 4.0 µg/mL alpha-factor mating pheromone (Zymo Research) and incubated at 30°C with shaking until the vast majority of cells in the population exhibited the ‘shmoo’ phenotype (2.5 hours) ...
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bioRxiv - Cancer Biology 2021Quote: ... Direct-zol™ RNA MiniPrep w/ Zymo-Spin™ IIC Columns (Zymo cat # D4019) were used to extract RNA from tumors ...
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bioRxiv - Molecular Biology 2023Quote: ... 38% v/v saturated acidic phenol) and the Direct-zol RNA MiniPrep Kit (including on-column DNase I treatment, Zymo Research, R2052) or according to the protocol for TRIzol™ Reagent (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... we used Zymo-Spin V-E (#C1024; Zymo Research) columns which are included in Zymo Research RNA extraction midi kits or can be purchased separately ...
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bioRxiv - Microbiology 2024Quote: ... 750 µl of lysis solution (ZymoBiomics Lysis Solution) and 19 µl 20 mg/ml proteinase K solution in storage buffer (Zymo Research, Irvine, CA, USA) were added into each tube and mixed ...
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bioRxiv - Immunology 2024Quote: ... and Direct-zol™ RNA MiniPrep w/ Zymo-Spin™ IIC Columns (Zymo Research, Irvine, CA). After purification from genomic DNA using DNase I (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and Direct-zol™ RNA MiniPrep w/ Zymo-Spin™ IIC Columns (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Selection for pyrF mutations was performed with 5-fluoroorotic acid (5-FOA, Zymo Research, part number F9001-5) at a final concentration of 0.5 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... transferred in the Zymo-Spin V column with Reservoir (Zymo Research), and centrifuged at 500 RCF for 5 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Direct-zol™ RNA MiniPrep w/Zymo-Spin™ IIC Columns (Zymo Research, Irvine, CA, USA). 1 μg of RNA from each sample was reverse transcribed using FastGene Scriptase Basic Kit (Nippon Genetics Europe ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Microbiology 2020Quote: ... a nucleic acid stabilizing solution from Zymo Research (R1100) ...
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bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
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bioRxiv - Plant Biology 2023Quote: ... Relative quantification of 5-methylcytosine (5-mC) over time was determined using the 5-mC DNA ELISA Kit (Zymo Research), using 100 ng of genomic DNA per sample as input ...
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bioRxiv - Genomics 2024Quote: ... Total RNA was then isolated using the Direct-zol RNA MiniPrep w/ TriReagent kit (Zymo Research R2053-A). Subsequently ...
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bioRxiv - Genetics 2023Quote: ... The PCR products were purified using Zymospin V columns (Zymo Research, C1016) and then by Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2024Quote: ... 5 µL DNA Digestion Buffer and 5 µL DNase I (Zymo Research; R1013) was added ...
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bioRxiv - Genomics 2022Quote: ... lower centrifugation speeds and the use of Zymo-Spin V columns (Zymo Research) rather than the Amicon columns)2 ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for the estimation of global 5-hmC and 5-mC levels by ELISA using the Quest 5-hmC ELISA kit and the 5-mC DNA ELISA kit (Zymo research Inc, USA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... pellet resuspended with 2 ml Host Depletion Solution (Zymo) and transferred without the beads to two 1.5 ml tubes per sample ...
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bioRxiv - Bioengineering 2024Quote: ... After adding 700 μL of wash solution (Zymo Research), the column was centrifuged again at 16,000 ×g for 30 s to remove the solution ...
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bioRxiv - Microbiology 2022Quote: Patient samples were collected using DNA/RNA Shield Lysis Tubes w/Swab (Microbe) (catalog number R1104; Zymo Research, California, USA). Sterile methods were used to collect pus samples from the infected breast ...
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bioRxiv - Genetics 2022Quote: ... RNA was extracted following instructions in the Quick-RNA™ MicroPrep Kit w/ Zymo-Spin™ IC Columns kit (Zymo Research).
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bioRxiv - Biochemistry 2023Quote: ... or a combination of TRIzol and the RNA Clean & Concentrator-5 (RCC-5) kit (Zymo Research), according to the Zymo instructions for combination Trizol/Zymo-Spin-Column RNA isolation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.075% 5-fluorooritic acid (Zymo Research)) ...
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bioRxiv - Genomics 2020Quote: ... We verified the presence of high molecular weight DNA products in the samples before purifying nucleic acids by Zymo DNA Clean & Concentrator-5 (ZYMO Research).
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bioRxiv - Synthetic Biology 2020Quote: ... and DCC-5 columns (Zymo Research). The sublibraries were pooled and sequenced on a single lane of a HiSeq4000 (Illumina) ...