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Citations for Zymo Research :
1 - 50 of 1173 citations for 7 16 DIHYDROBENZO A BENZO 5 6 QUINO 3 2 I ACRIDINE 9 18 DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 5 μl DNase I (6 U/μl) (Zymo Research) and 75 μl DNA Digestion Buffer (Zymo Research ...
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bioRxiv - Genomics 2020Quote: ... DNA in the initial 20-μL nucleic acids solution was digested by adding 16-μL DNase I (1 U/μL) plus 4-μL 10X DNase I buffer (DNase I set; Zymo Research), incubating for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: Three embryos at neural plate (st. 16-18) or three dissected heads at larval (st. 28-34) stages were collected in TRI-reagent (Zymo) and processed for RNA extraction with DNAse I treatment using the Direct-zol RNA Miniprep kit (Zymo) ...
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bioRxiv - Microbiology 2023Quote: ... 2 mock community samples (Zymo Research DNA standard I D6305), 2 negative DNA extraction samples ...
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bioRxiv - Microbiology 2023Quote: ... 2 mock community samples (Zymo Research DNA standard I D6305), 2 negative DNA extraction samples ...
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bioRxiv - Genomics 2020Quote: ... concentrated (RNA Clean & Concentrator™-5 with DNase I, Zymo Research), and used for library preparation (Trio RNA-Seq ...
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bioRxiv - Molecular Biology 2020Quote: ... treated for two hrs using 2 units of DNase I per hour and was cleaned of residual DNase I with the RNA clean and concentrator-5 (Zymo). DNase-treated RNA was then divided into two equal aliquots ...
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bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
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bioRxiv - Microbiology 2020Quote: ... 0.5% Triton X-100 in PBS) containing 5–10 U DNase I (Zymo Research) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... DNase I-digested and cleaned using R1014 RNA Clean & Concentrator-5 kit (Zymo Research), cDNA synthesized using the qScript Flex cDNA kit (95049 Quanta ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then treated with Turbo DNAse 2 to 3 times and then purified with the RNA Clean and Concentrator-5 Kit (Zymo Research Cat#: 11-325) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... 0.25 μL of Proteinase K (Zymo, D3001-2-5), and 2.5μL of H2O ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 and 4 by lysing cells in the 6-well plate in RNA lysis buffer (Zymo) and freezing at −80 °C until extraction ...
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bioRxiv - Microbiology 2023Quote: ... the reaction was prepared in a volume of 20 µL consisting in 2 µL of the template DNA and 18 µL of Femto Bacterial qPCR Premix (Femto Bacterial DNA Quantification Kit, Zymo Research). The PCR reaction was performed using a 7500 Fast Real-Time PCR System (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... DNase I treated and concentrated using the RNA Clean & Concentrator™ −5 Kit (#R1014, Zymo Research). Total RNA (1μg ...
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bioRxiv - Plant Biology 2021Quote: ... Lysis tubes were shaken 5 min at 16 Hz using a ball mill to extract and purify DNA following the manufacturer’s guidelines (Zymo Research). Microbiome profiling of isolated DNA samples was performed by Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
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bioRxiv - Neuroscience 2020Quote: ... and sorted into 384-well plates preloaded with 2μl of digestion buffer for snmC-seq215 (20 mL digestion buffer consists of 10 mL M-digestion buffer (2×, Zymo D5021-9), 1 ml Proteinase K (20 mg ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... DNase I treated RNA samples were purified and further concentrated using RNA clean and concentrator-5 (Zymo Research, R1014) according to the manufacture’s protocols ...
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bioRxiv - Neuroscience 2022Quote: ... and 16S targeted sequencing was completed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA) and V3-V4 16S primers (Zymo Research ...
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bioRxiv - Plant Biology 2021Quote: ... RNA samples were digested with DNase I and purified using RNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... The cells debris was removed after centrifugation at 15000g for 5 mins and cell supernatant containing the released virions were subjected to 5μl DNase I (Zymo research) for 1 hour to remove remaining untransfected chimeric NL plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... DNase I (Zymo Research) treatment was performed on-column according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... FAIRE DNA was next purified by Zymo-I spin columns (Zymo) and detected by qPCR assay.
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bioRxiv - Synthetic Biology 2021Quote: ... Dnase I (Zymo Research) was added to degrade linear DNA template ...
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bioRxiv - Microbiology 2020Quote: ... The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene using primers custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated for 16 hours at 16 °C and were terminated by cleaning with RNA Clean & Concentrator (Zymo Research). Crosslink reversal was done by irradiating the RNA on ice with 2.5 KJ/m2 254 nm UVC using a CL-1000 crosslinker (UVP).
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bioRxiv - Immunology 2023Quote: ... Bacterial 16S rRNA gene targeted sequencing was performed using the Quick-16S NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Developmental Biology 2021Quote: ... and supplemented with 1mg/mL Proteinase K (Zymo Research, Cat#: D3001-2-5) for 1 hour at 55°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 ul M-Digestion Buffer and 1ml Proteinase K (Zymo D3001-2-5) were added and incubated for 20 minutes at 50°C ...
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bioRxiv - Molecular Biology 2023Quote: ... cfRNAs were then purified and concentrated into 6 µl using an RNA Clean and Concentrator-5 kit (Zymo, R1016). Plasma extracellular vesicles (EVs ...
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bioRxiv - Microbiology 2021Quote: ... 16S ribosomal RNA gene-targeted sequencing was performed using a Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA), in which 16S primers were used to amplify the V3/V4 region of the 16S rRNA gene ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA sequencing libraries were prepared by amplifying and barcoding the V1-V2 region of the 16S rRNA gene using the Quick-16S NGS Library Prep Kit (Zymo Research). Samples were pooled and sequenced on the Illumina MiSeq platform with 2 × 250 base pair standard run at Washington University DNA Sequencing Innovation Lab.
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bioRxiv - Physiology 2021Quote: ... bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S NGS Library Preparation Kit (Zymo Research, Irvine, CA). The bacterial 16S primers ...
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bioRxiv - Physiology 2022Quote: Total RNA was extracted from larval fat bodies (2-5 pooled/sample) or larval brains (5 pooled/sample) using the Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
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bioRxiv - Genetics 2019Quote: ... Total RNA was isolated from cell pellets in 5 batches (each batch contained one of each of the 5 strains) using the ZR Fungal/Bacterial RNA mini-prep kit including the DNase I on-column digestion step (Zymo Research). Each cell pellet was resuspended in lysis buffer and transferred to screw-capped tubes containing glass beads and the cells were broken open using a mini-beadbeater (Biospec Products ...
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bioRxiv - Genomics 2020Quote: ... The reaction was then treated with DNase I to remove template DNA and RNA product was purified using an RNA Clean and Concentrator-5 (Zymo #R1016). Purified RNA was used as the template for reverse transcription using ProtoScript II according to manufacturer protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... After the second treatment with DNase I the samples were column purified and concentrated using RNA Clean & Concentrator-5 kit (Zymo Research). Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
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bioRxiv - Genomics 2022Quote: ... The samples were treated with DNase I and cleaned using ethanol precipitation and subsequent RNA Clean & Concentrator-5 spin-column purification (Zymo Research). Successful removal of genomic DNA was confirmed by PCR using a host-specific primer pair (Supplementary Figure S1) ...
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bioRxiv - Microbiology 2020Quote: ... Most of the supernatant was removed and 750 μl of ZymoBIOMICS Lysis Solution and 19 μl of proteinase K (D3001-2-20/D3001-2-5, Zymo Research) were added to the remaining 200 μl of the samples and incubated for 30 min at +55 °C ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene using primers custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
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bioRxiv - Microbiology 2021Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Bacterial 16S primers amplify the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Molecular Biology 2023Quote: TM4 and C-terminal tail point mutations were made by digesting Asu-ACR-16-REM and Dme-ACR-16-REM with Bsu15I and ApaI (ThermoScientific, USA) followed by gel purification (Zymo Research, USA). These were then ligated to oligo inserts containing the TM4 and C-terminal tail point mutations (S Table 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5μL DNase I (Zymo #R1014) and 35μL of DNA Digestion Buffer (Zymo #R1014 ...
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bioRxiv - Genomics 2024Quote: ... the annealed oligos were incubated with 10 mM 5-hydroxymethyl-2’-dCTP (Zymo Research), along with dGTP ...
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bioRxiv - Neuroscience 2022Quote: ... and V3-V4 16S primers (Zymo Research, Irvine, CA). The sequencing library was prepared using real-time PCR ...
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bioRxiv - Genomics 2019Quote: ... and RRBS Libraries were prepared from 40 ng of genomic DNA digested with Msp I and then extracted with DNA Clean & Concentrator™-5 kit (Zymo Research). Fragments were ligated to pre-annealed adapters containing 5’-methyl-cytosine instead of cytosine according to Illumina’s specified guidelines (www.illumina.com) ...
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bioRxiv - Synthetic Biology 2021Quote: Plasmids were isolated from 5-10 mL yeast cultures either by alkaline lysis (39) or using Zymo Yeast Miniprep Kit I (Zymo Research D2001). Plasmids were then recovered into DH10B ElectroMax E ...