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Citations for Zymo Research :
201 - 250 of 378 citations for 6 Phenyl piperidine 1 3 dicarboxylic acid 1 tert butyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... linear PCR products were purified from agarose gel (1%) with a Zymoclean-Gel DNA Recovery kit (Zymo Research) and circularized with DNA Ligation Kit ‘Mighty Mix’ (Takara) ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour at room temperature followed by isolation with a RNA Clean & Concentrator-25 kit (Zymo Research). 1 µg RNA was converted to ribosomal depleted cDNA libraries ready for sequencing using the TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat kit (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng-1 μg of genomic DNA was bisulfite converted using the EZ DNA Methylation-Gold kit (Zymo). Bisulfite-converted DNA was PCR amplified ...
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bioRxiv - Microbiology 2023Quote: ... 70∼80% cells were positive for Thy1.1 after sorting and lysed with TRI reagent (Zymo Research #R2050-1) for RT-qPCR.
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bioRxiv - Cell Biology 2024Quote: ... After purifying both PCR products using a 1% agarose gel and a gel purification kit (Zymo, no. D4007), the two fragments were assembled using a Gibson assembly mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... the PCR master mix has been treated with 0.02% (v/v) of DNAse I 1 U/mL (Zymo) at room temperature for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: The double stranded deoxyribonucleic acids (dsDNA) was extracted from the duplicate sludge samples using ZR soil microbe DNA kit (Zymo Research, CA., USA), according to manufacturers instructions ...
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bioRxiv - Microbiology 2020Quote: ... the nucleic acids were resuspended in DEPC-treated nuclease-free water purified with the OneStep PCR Inhibitor Removal Kit (Zymo Research, Irvine, USA), then split into a fraction for DNA and one for RNA ...
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bioRxiv - Microbiology 2020Quote: ... The YAC DNA was extracted using spin column-based nucleic acid purification (ZR BAC DNA Miniprep Kit, ref. D4049, Zymo Research, Irvine, CA). The YAC containing viral cDNA was transformed into E ...
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bioRxiv - Synthetic Biology 2020Quote: ... were pretreated with a solution of β-mercaptoethanol plus 60 mM EDTA and then incubated with 6 U/mL zymolyase (Zymo Research) in Buffer I to remove the cell wall ...
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bioRxiv - Microbiology 2022Quote: ... 6-10 aphids colonized with fluorescent bacteria from each condition were collected and crushed in DNA/RNA shield (Zymo Research, CA, USA). Whole aphid RNA was purified using an RNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Cancer Biology 2023Quote: In vitro-infected keratinocytes and SCC cells were lysed in 6-wells with 1mL TRIzol Reagent and the RNA was purified by using the Direct-zol™ RNA Miniprep kit (Zymo Research). The procedure was performed according to the manufacturer protocol except for an additional 1 min centrifugation after the last washing step to completely remove eventual ethanol leftover ...
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bioRxiv - Plant Biology 2023Quote: ... Each biological replicated was generated by pooling three individual ChIP pull-downs (each consisting of 6 g of whole seedling tissues) during DNA purification using the ChIP DNA Clean & Concentrator Kit (Zymo Research, D5201). The samples were sent to BGI Genomics for library preparation and sequencing using DNBSEQ ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from three biological replicates of vehicle-treated (Veh) and 48 h-TTX-treated (TTX) neurons (6 samples in total) were isolated using Quick-RNA Microprep kit (Zymo Research, R1051) according to the instructions of manufacturer ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from 3 x 105 Raji cells using Direct-zolTM RNA MiniPrep kit (Zymo Research) according to manufacturers’ instructions ...
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bioRxiv - Physiology 2024Quote: ... and KI-TTL mice (N=3 per group) using the Direct-zol RNA Microprep Kit (Zymo Research) followed by a DNase digestion step ...
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bioRxiv - Evolutionary Biology 2022Quote: ... strains were struck to synthetic defined (SD) media (CSM minus arginine, yeast nitrogen base, glucose) containing 5-Fluoroorotic Acid (5FOA) (Zymo Research, Irvine, CA, USA). to isolate cells that spontaneously lost pTC416-SEC53 ...
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bioRxiv - Genomics 2021Quote: Total nucleic acids were extracted from 300 μL of nasopharyngeal samples and eluted with 60 μL sterile RNase-free water using a commercial kit (Zymo total nucleic acid, USA). Total NA concentration was assayed by Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were run on 1% agarose gel and purified using the Zymoclean gel DNA recovery kit (Zymo Research).
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bioRxiv - Microbiology 2022Quote: ... RNA extractions were performed from 1 ml LB culture using the Direct-zol RNA MiniPrep Plus (Zymo Research, ZR2073). Total RNAs were reverse-transcribed into cDNAs using iScript™ cDNA Synthesis Kit (BioRad ...
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bioRxiv - Systems Biology 2022Quote: ... The flow through was mixed with 1 volume of ethanol then transferred to a Zymo Spin IIC column (Zymo) and centrifuged ...
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bioRxiv - Biochemistry 2020Quote: ... The entire PCR reaction is run on 1% agarose gel and the DNA at 230bp was purified (Zymo Research). See Supplemental Figure S2.
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bioRxiv - Immunology 2021Quote: ... Cxcl1 promoter regions #1 and #2 were PCR amplified from sodium bisulfite converted gDNA using ZymoTaq Polymerase (Zymo Research) and purified by gel extraction using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae eby100 was retransformed using 1 µg of pGal1 – Aga2p_eGFP following the protocol of the EZ yeast transformation kit II (Zymo). 100 µL of cells were selected on SDCAA plates ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant and pellet samples on non-denaturing 1% agarose gel we extracted RNA with TRI reagent® (ZYMO Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant and pellet samples on non-denaturing 1% agarose gel we extracted RNA with TRI reagent® (ZYMO Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL ...
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bioRxiv - Plant Biology 2023Quote: RNA was purified from seedlings Col-0 and cpk32-1 using the Direct-zol RNA MiniPrep Kit (Zymo Research). The reverse-transcriptase cDNA synthesis reaction was carried out by the RevertAid Reverse Transcriptase (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: Bacterial RNA was extracted from ∼1 × 108 CFU after AMP exposure (above) using Direct-zol RNA Miniprep kit (Zymo) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... One of each type of swabs was placed into 1 mL DNA/RNA Shield (Zymo Research, Irvine California, USA) and the other of each into 1 mL methanol ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-500 ng of DNA from each sample was bisulfite-converted using the Zymo EZ DNA Methylation Kit (Zymo Research, Irvine, CA USA) following the manufacturer’s protocol and the specified modifications for the Illumina Infinium methylation assays ...
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bioRxiv - Bioengineering 2021Quote: Genomic DNA was extracted from 3×106 HEK293_LPCD19/BiTE cells using Quick-DNA midiprep kit (#D4075, Zymo Research). Gene specific primers annealing to the signal sequence (5’-CTGGTCCTGCATCATCCTGTTTC-3’ ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... We then lysed 3 dpf larvae individually by rigorously pipetting in 55 μl DNA/RNA Shield (Zymo Research) at RT followed by overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and were filled with 3 µl of the RNA Lysis Buffer (Quick-RNA Microprep Kit, Zymo, Irvine, Ca). The pipette was mounted on a micromanipulator and used to aspirate 2– 10 corpuscles from duck bill skin by applying negative pressure using a high-speed pressure clamp system (HSPC-1 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Then 1 mL of the culture was removed for DNA extraction using the Zymoprep yeast Plasmid Miniprep II (Zymo Research) kit ...
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bioRxiv - Bioengineering 2020Quote: ... at 37°C for 1 hour to remove the gblock template then purified using a RNA spin column (Zymo Research). Purified RNA was then treated with 200 units of calf intestinal phosphatase (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: 1 μg of genomic DNA was hydrolyzed overnight at 37 ºC with 5 U of DNA Degradase Plus (Zymo Research) following the manufacturer’s protocol in 50 μl volume ...
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bioRxiv - Synthetic Biology 2021Quote: For changing cation concentration from 1 to 0 we utilized the ssDNA clean-up kit (ssDNA/RNA clean/concentrator D7010) from Zymo Research such that all the extended ssDNA synthesized in the initial part of the experiment was retained on the column and the TdT ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pQE60::trpCp variants were transformed into the ΔtrpD JW1255-1 strain (BW25113) from the KEIO collection 47 using the Mix & Go! protocol from Zymo Research (Zymo Research ...
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bioRxiv - Systems Biology 2021Quote: ... at which point a 200 µL aliquot was removed and preserved in 1 mL of DNA/RNA Shield (Zymo Research) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Metagenomic libraries were diluted to 1 nM using the Select-a-Size DNA Clean and Concentrator MagBead Kit (Zymo Research) and pooled for further sequencing on the Illumina MiniSeq platform ...
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bioRxiv - Genomics 2022Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted by vortexing for 30 min in elution buffer (1% SDS and 0.1 M NaHCO3) and DNA was purified using a ChIP DNA Clean & Concentrator kit (Zymo Research). For each ChIP ...
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bioRxiv - Synthetic Biology 2020Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Biophysics 2021Quote: ... Sufficient amounts of DNA were obtained growing 1 L of transfected XL1 Blue Escherichia coli cells overnight and performing a gigaprep (Zymo). For protein expression ...
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bioRxiv - Evolutionary Biology 2019Quote: 208 female beetles (Table 1) p stored at −80°C were homogenized in Tri-Reagent® (Zymo Research, California, USA) using an electric bead mill ...
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bioRxiv - Genomics 2021Quote: Total RNA was purified in duplicate from 1 million frozen cells using the Direct-zol RNA MiniPrep kit from Zymo Research (#R2050) ...
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bioRxiv - Genetics 2020Quote: ... total RNA from approximately 1 × 108 cells was extracted using the Quick-RNA Fungal/Bacterial Miniprep (Zymo Research, Irvine, CA). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were vortexed for 10 seconds in 300 μL of 1 × DNA/RNA Shield™ (Zymo Research, Cat. No. R1100) and centrifuged for 5 minutes at 5,000 × g ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and DNA was extracted at days 1 and 5 utilizing the Quick-DNA/RNA Miniprep (Zymo Research). Viral genomes were quantified using a standard curve and normalized to RNAseP.