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Citations for Zymo Research :
1 - 50 of 1448 citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... we dissected embryonic tails at E12.5 (n = 4) and E14.5 (n = 4) and extracted RNA using 50 µl Direct-zol (Zymo Research) following the manufacturer’s protocol and used the same library preparation procedures as for the parental samples ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA from 105 days old CorticOs (N = 4; n = 6-8 per genotype) was extracted using the Direct-zol RNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 and 4 by lysing cells in the 6-well plate in RNA lysis buffer (Zymo) and freezing at −80 °C until extraction ...
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bioRxiv - Genetics 2023Quote: ... Microinjections were performed with 4-5 µg/µl (diluted in nuclease free water, Zymo Research) fas5 and GFP dsRNA on a Femtojet microinjector (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from late third instar fat bodies (n = 4-6 pooled/sample) using a Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
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bioRxiv - Microbiology 2023Quote: ... control samples (n = 4, one for each extraction batch) using commercially available kits optimized for fecal-based microbial communities (Zymo fecal/soil kit), and some aliquots archived ...
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bioRxiv - Genomics 2022Quote: ... followed by max speed centrifugation at 4 °C for 5 minutes and DNA purification by column purification (Zymo) prior to sample preparation for next-generation sequencing.
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from the PMA-treated soil samples (n = 48) and the no-template (water) controls (n = 4) using the Quick-DNA Fecal/Soil Kit (Zymo Research, Freiburg, Germany), which is designed explicitly for soil-based microbial communities ...
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bioRxiv - Microbiology 2023Quote: ... 4 ml of urine was added to 4 ml of 2X DNA/RNA Shield® (Zymo, USA). Serum and urine samples were stored and transported on ice to the Institute of Microbiology at Universidad San Francisco de Quito and thereafter maintained at −20°C until DNA extraction.
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bioRxiv - Developmental Biology 2024Quote: ... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... ∼4-6×109 cells were concentrated and suspended in 500 μl of DNA/RNA Shield (Zymo Research) in 2 mL screw cap tubes ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl DNase I (6 U/μl) (Zymo Research) and 75 μl DNA Digestion Buffer (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: Total RNAs were extracted from BMDMs (male mice: 4 Cre+ and 4 Cre−) using the Quick-RNA miniprep plus kit (Zymo). With a minimum of 300 ng input RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... nucleus accumbens and lung of the 4 juvenile and 4 old female mice was bisulfite converted using the EZ DNA methylation Gold kit (Cat# D5006, Zymo Research) according to manufacturer’s instructions and eluted ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from rosette tissue harvested at ZT 23 on day 25 post imbibition (n = 4 biological replicates) using the Direct-zol-96 MagBead RNA kit (Zymo Research). RNA was checked for degradation using a Tapestation 4150 (Agilent ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was isolated from the fresh feces collected from UT1- or PBS-gavaged mice (female, n = 4/group) using a ZymoBIOMICS DNA Miniprep Kit (Zymo Research). Note ...
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bioRxiv - Biochemistry 2024Quote: ... at 37 °C for specific time (20 min, 1 hour, 2 hours, 4 hours, or 16 hours) followed by Oligo Clean & Concentrator Kits (OCC, Zymo Research, D4061) cleanup ...
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bioRxiv - Developmental Biology 2024Quote: 20 zebrafish embryos per biological replicate were collected at 4 or 6 hpf and snap-frozen in 300 µl of DNA/RNA Shield (#R1100-50, Zymo Research). Total RNA then was extracted using Direct-zol RNA Miniprep Kit (#R2050 ...
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bioRxiv - Genetics 2023Quote: ... we extracted DNA from 2–4 dpf whole larvae using the Quick-DNA Microprep Kit (D3021, Zymo Research). We then performed a PCR for GFP as well as a ‘junction PCR’ to amplify a region including both mitfa and GFP to ensure integration at the intended locus.
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bioRxiv - Immunology 2024Quote: ... and 3×104-5×104 cells were collected for RNA isolation (RNA Clean & Concentrator-5, Zymo # R1013). The isolated RNA was hybridized with a nCounter custom probe set (108 probes ...
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bioRxiv - Genomics 2022Quote: ... 0.25 μL of Proteinase K (Zymo, D3001-2-5), and 2.5μL of H2O ...
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bioRxiv - Genomics 2020Quote: ... The genomic DNA of JLat cells and CD4 T cells from individuals #3 and #4 were extracted with a Quick-DNA Miniprep Plus kit (Zymo Research #D4068) according to the product protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mixture was incubated at 65 °C for 3 h and the RNA was then isolated by using the Zymo RNA clean and concentrator-5 kit (Zymo Research, cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting product was purified using 4 spin columns (Zymo, DCC-25) to produce the round 1 product.
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bioRxiv - Pathology 2024Quote: ... and 4 tonsils) with Quick-DNA/RNA FFPE Miniprep Kit (Zymo Research). Barcoded RNA-seq library was established with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified one last time using Zymo RNA Clean and Concentrator-5 (Zymo Research R1013). Capped RNA concentrations were measured using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific Q32852) ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA and RNA were extracted from fresh frozen tumor (n=2) and normal (n=3) samples using the ZR-Duet DNA/RNA MiniPrep extraction kit (ZYMO RESEARCH, Irvine, CA). Quantification was performed with a NanoDrop One™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
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bioRxiv - Genomics 2023Quote: ... The DNA pellet was eluted in elution buffer (D3004-4-10 Zymo Research) and incubated at 50°C for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... Libraries were eluted in DNA Elution Buffer (Zymo Research, Cat #D3004-4-10). The concentration and average size of the libraries was verified using the TapeStation DNA 5000 Assay Reagents and Screen Tapes (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... Circular RNA was purified through RNA Clean & Concentrator(tm)-5 column (Zymo research, Cat. n° R1013) and quantified using Qubit(tm ...
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bioRxiv - Physiology 2022Quote: Total RNA was extracted from larval fat bodies (2-5 pooled/sample) or larval brains (5 pooled/sample) using the Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
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bioRxiv - Genomics 2024Quote: ... The DNA pellet was eluted in an elution buffer (D3004-4-10 Zymo Research) and incubated at 50°C for 10 minutes ...
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bioRxiv - Pathology 2024Quote: ... Adapter-ligated fragments of 150-250bp and 250-300bp were recovered from a 2/5% NuSieve 1:1 agarose gel (Zymoclean Gel DNA Recovery Kit, Zymo Research). The fragments were then bisulfite treated using EX DNA Methylation-Lightning Kit (Zymo Research) ...
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bioRxiv - Bioengineering 2024Quote: Cell number was directly assessed as a function of total isolated DNA at experimental Days 1 and 7 using a Zymo Quick-DNA Microprep Plus Kit (n=5, Zymo Research Crop, Irvine, CA). A standard curve at experimental Day 0 was generated using known quantities of hMSCs and following the “Biological Fluids & Cells” protocol as directed ...
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bioRxiv - Genomics 2020Quote: ... DNA in the initial 20-μL nucleic acids solution was digested by adding 16-μL DNase I (1 U/μL) plus 4-μL 10X DNase I buffer (DNase I set; Zymo Research), incubating for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Golden Gate reactions for all-in-one vector assembly were purified with DNA Clean & Concentrator-5 (Zymo Research) and electroporated into E ...
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bioRxiv - Microbiology 2023Quote: ... long RNA species (>200bp) were separated from short ones (<200) using RNA Clean & Concentrator-5 kit (Zymo, R1013).
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bioRxiv - Genomics 2021Quote: ... Pellets were then resuspended in Zymo DNA elution buffer (Zymo, Cat No: D3004-4-10), and placed on the heat block at 65°C for 1 hour to completely dissolve the genomic DNA.
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bioRxiv - Genomics 2024Quote: ... Pellets were then resuspended in Zymo DNA elution buffer (Zymo, Cat No: D3004-4-10), and then incubated at 65°C for 1 hour to completely dissolve the genomic DNA.
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bioRxiv - Microbiology 2024Quote: ... 75ng of DNA isolated using both lab types (n=6) and the Zymobiomics microbial community standard was used as a control (n=1) (Zymo Research Corporation, Irvine, CA, USA). Sequencing of all samples was run for 24 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... and supplemented with 1mg/mL Proteinase K (Zymo Research, Cat#: D3001-2-5) for 1 hour at 55°C ...
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bioRxiv - Bioengineering 2021Quote: ... Selection for pyrF mutations was performed with 5-fluoroorotic acid (5-FOA, Zymo Research, part number F9001-5) at a final concentration of 0.5 mg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... total RNA was isolated at 4 hpf using Direct-zol RNA Miniprep Kit (#R2050, Zymo Research) following manufacturer’s instructions and quantified using the Qubit fluorometric quantification (#Q10210 ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C) and flowed over 1.1 mL volume streptactin column (Streptactin XT superflow, Zymo Research, ZRE160702). Flow through was collected and added to the column a second time ...
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bioRxiv - Genomics 2023Quote: ... and gDNA wash buffers from Zymo (#D3004-1, #D3004-5). DNA library preparation was also performed in 96-well plates ...
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bioRxiv - Molecular Biology 2024Quote: ... Strep-Tactin resin (Zymo Research, catalog number P2004-1-5), or S-protein agarose (Novagen ...