Labshake search
Citations for Zymo Research :
51 - 100 of 106 citations for 5α CHOLESTAN 3 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Genomic DNA was extracted from 3×106 HEK293_LPCD19/BiTE cells using Quick-DNA midiprep kit (#D4075, Zymo Research). Gene specific primers annealing to the signal sequence (5’-CTGGTCCTGCATCATCCTGTTTC-3’ ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... We then lysed 3 dpf larvae individually by rigorously pipetting in 55 μl DNA/RNA Shield (Zymo Research) at RT followed by overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and were filled with 3 µl of the RNA Lysis Buffer (Quick-RNA Microprep Kit, Zymo, Irvine, Ca). The pipette was mounted on a micromanipulator and used to aspirate 2– 10 corpuscles from duck bill skin by applying negative pressure using a high-speed pressure clamp system (HSPC-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Infectious viral stocks were inactivated by a 1:3 dilution with TRI Reagent® (Zymo, R2050-1-200) immediately prior to RNA extraction ...
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bioRxiv - Bioengineering 2019Quote: The 3-D centrifuge was used to perform DNA extractions using the Quick-DNA Miniprep Plus kit (Zymo Research) according to the protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA products were separated on a 3% agarose gel and then extracted using gel extraction kit (ZYMO research). In vitro transcription with T7 RNA polymerase and in vitro Dicer cleavage assays were performed as previously described (Sheng et al ...
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bioRxiv - Bioengineering 2019Quote: Total RNA was isolated from 2-3 million HAP1 cells using the Direct-zol-96 RNA kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: Total RNA was isolated from 2-3 million HAP1 cells using the Direct-zol-96 RNA kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from 3×105 cells per replicate using Direct-Zol RNA Miniprep Kit (Zymo Research, USA) according to the manufacturer’s protocol and shipped in dry ice to the MDC/BIH Genomic Core Facility for quality assessment using TapeStation (Agilent Technologies) ...
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bioRxiv - Systems Biology 2023Quote: ... 3 μl of reaction mix were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... the total RNA was extracted from active and tun samples (3 replicates) using Direct-Zol RNA MicroPrep Kits (Zymo Research), and the sequencing library was constructed using SMARTer v4 Ultra Low Input RNA Kit for Sequencing (Clonetech ...
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bioRxiv - Developmental Biology 2019Quote: ... The Pgap2 exon 3 variant was Sanger sequenced using the Zymo DNA clean & Concentrator kit (Zymo Research Corporation, Irvine, CA).
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bioRxiv - Genetics 2022Quote: Yeast colonies containing bait and prey constructs that grew on Trp−/Leu−/His− + 25 mM 3-Amino-1,2,4-triazole (3AT) selection plates were extracted using the Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004) followed by Sanger sequencing for gene identification ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
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bioRxiv - Genomics 2023Quote: ... 3 colonies were randomly selected and further expanded for plasmid DNA purification (Zyppy™ Plasmid Purification Kit; Zymo research #D4019). Extracted DNA was then sequenced to verify the integrity of the cloned fragments.
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from 3 biological replicates of 100mg of frozen tissue of each accession using the Quick-DNA/RNA Miniprep kit (Zymo) following manufacturer’s instructions with the following modifications ...
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bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Genetics 2019Quote: 25-30 eyes were dissected from both WT and pitx2ΔCE5-7 eye zebrafish embryos at 3 dpf in Ringer buffer and genomic DNA was purified using Quick-DNA microprep Plus kit (ZYMO Research). Eyes were resuspended in Solid Tissue buffer containing Proteinase K and incubated at 55°C for 6 hours ...
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bioRxiv - Microbiology 2019Quote: ... at 48 hpe cells were washed 3 times with PBS to eliminate extracellular virus and total RNA was extracted using Direct-zol RNA MiniPrep Plus (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Individual 50 μL reactions were run on a 3% agarose gel and libraries were purified using the Zymoclean Gel DNA Recovery kit (D4007, Zymo Research). Sequencing was performed using standard Illumina instructions ...
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bioRxiv - Microbiology 2022Quote: ... and stored at -20°C until (≤ 3 days) the DNA was extracted via the Zymo Quick-DNA Fecal/Soil Microbe kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
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bioRxiv - Molecular Biology 2019Quote: ... the DNA was treated with sodium bisulfite under non-denaturing conditions (no denaturation and incubation at 37°C for 3 hrs) using the Zymo Lightning bisulfite modification kit (Zymo Research). Following sample clean-up ...
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bioRxiv - Genomics 2021Quote: ... The nascent RNA was purified by adding 3 volumes of TRIzol and processed with the Direct-zol RNA Miniprep kit (Zymo Research) with addition of 1/100 volume of 100 mM DTT to each supplied wash buffer.
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bioRxiv - Microbiology 2021Quote: ... and genomic DNA was extracted from 3 mL of overnight (TSB) broth culture using the ZR Duet DNA/RNA MiniPrep Plus kit (Zymo Research) following manufacturer’s protocol for the “suspended cells” option ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Microbiology 2022Quote: ... The high molecular weight bands were excised with a razor and dissolved in 3 volumes of Zymo Agarose Dissolving Buffer (Zymo D4001) at 50°C ...
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bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
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bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Genomics 2020Quote: ... The genomic DNA of JLat cells and CD4 T cells from individuals #3 and #4 were extracted with a Quick-DNA Miniprep Plus kit (Zymo Research #D4068) according to the product protocol ...
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bioRxiv - Genomics 2022Quote: ... into the 20 μl ligation reaction for incubation with 30 minutes at 30°C and 3 minutes at 65°C to inactivate the enzymes followed by column purification (Zymo Research, R1016). Before the reverse transcription (RT) ...
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bioRxiv - Plant Biology 2020Quote: Genomic DNA was extracted from aerial parts of 3-week-old seedlings using Quick-DNA Plant/Seed Miniprep Kit (Zymo research, D6020) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: A PCR of all constructs using forward primer pTD2F (5’-TTGGCACCAAAATCAACGGG–3’) and reverse primer SP6 was carried out with SuperFi DNA Polymerase (ThermoFischer Scientific, USA) and agarose gel purified (Zymo Research, USA). These primers amplify the gene insert ...
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bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
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bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
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bioRxiv - Zoology 2024Quote: ... Clean-up used a combination of two kits: the beads from the Select-a-size Magbead kit (Zymo Cat# D4084-3-10) and the buffers from Genomic DNA Clean & Concentrator -25 (Zymo Cat# D4064) ...
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bioRxiv - Physiology 2024Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick-RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
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bioRxiv - Physiology 2023Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick- RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA and RNA were extracted from fresh frozen tumor (n=2) and normal (n=3) samples using the ZR-Duet DNA/RNA MiniPrep extraction kit (ZYMO RESEARCH, Irvine, CA). Quantification was performed with a NanoDrop One™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... the aqueous phase was diluted back in TRizol reagent (1:3) to proceed with RNA purification by zymodirect RNA kit (Zymo research, cat. R2051). The quality of RNA samples was assessed using Agilent 2200 TapeStation (Agilent Technologies ...
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bioRxiv - Physiology 2020Quote: ... from all the experimental groups (n=3/group) was subjected to bisulphite treatment using the EZ DNA methylation™ kit (Zymo Research, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... one set of replicates (total of 3 replicates per treatment) was harvested for DNA and RNA using ZR-Duet DNA/RNA MiniPrep (Plus) kit (Zymo Research Cat # D7003) following manufacturer’s protocol ...
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Phage-centric ecological interactions in aquatic ecosystems revealed through ultra-deep metagenomicsbioRxiv - Microbiology 2019Quote: ... were cut in small pieces (≅ 3–5mm) using sterile scissors and processed for DNA extraction using the ZR Soil Microbe DNA MiniPrep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and 3 swabs (2 for metagenomic DNA isolation, 1 for storage) moistened with DNA/RNA shield (Zymo Research, Cat. No. ZYR.R1100-250). Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e ...