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Citations for Zymo Research :
1 - 50 of 1307 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.075% 5-fluorooritic acid (Zymo Research)) ...
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bioRxiv - Bioengineering 2021Quote: ... 10 mL 5-Fluoroorotic Acid (Zymo Research), 50 mg uracil (MP Biomedicals 103204) ...
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bioRxiv - Developmental Biology 2024Quote: ... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Bioengineering 2021Quote: ... Selection for pyrF mutations was performed with 5-fluoroorotic acid (5-FOA, Zymo Research, part number F9001-5) at a final concentration of 0.5 mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... selective media with 5-fluoroorotic acid (Zymo Research, Tustin, CA), or YPD selective media (YPD G418+) ...
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bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
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bioRxiv - Molecular Biology 2023Quote: ... Infectious viral stocks were inactivated by a 1:3 dilution with TRI Reagent® (Zymo, R2050-1-200) immediately prior to RNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Genomics 2023Quote: ... and gDNA wash buffers from Zymo (#D3004-1, #D3004-5). DNA library preparation was also performed in 96-well plates ...
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bioRxiv - Molecular Biology 2024Quote: ... Strep-Tactin resin (Zymo Research, catalog number P2004-1-5), or S-protein agarose (Novagen ...
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bioRxiv - Molecular Biology 2020Quote: ... all samples’ total nucleic acid (TNA) were treated with DNAse 1(Zymo Research, Catalog # E1010), which cuts both double-stranded and single-stranded DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... The nucleic acid was purified using a RNA Clean & Concentrator-5 (Zymo Research) and eluted in 7µL H2O.
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bioRxiv - Cancer Biology 2020Quote: Approximately 1.4kb of genomic fragment spanning SALL4 5’ UTR - exon 1 - intron 1 was PCR amplified (Zymo Research) and cloned into the pGEM-T Easy vector ...
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bioRxiv - Immunology 2024Quote: ... and 3×104-5×104 cells were collected for RNA isolation (RNA Clean & Concentrator-5, Zymo # R1013). The isolated RNA was hybridized with a nCounter custom probe set (108 probes ...
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bioRxiv - Pathology 2024Quote: ... Adapter-ligated fragments of 150-250bp and 250-300bp were recovered from a 2/5% NuSieve 1:1 agarose gel (Zymoclean Gel DNA Recovery Kit, Zymo Research). The fragments were then bisulfite treated using EX DNA Methylation-Lightning Kit (Zymo Research) ...
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bioRxiv - Microbiology 2022Quote: ... samples were homogenized 3:1 weight:volume in DNA/RNA Shield® (Zymo® Research Inc.).
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... at a ratio of 1:3 (DNA : Zymo Shield / 20 µl DNA:60 µl Zymo Shield). O volume of 5µl of the PCR product was loaded on a gel for confirmation of the reaction ...
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bioRxiv - Synthetic Biology 2022Quote: ... at a ratio of 1:3 (DNA : Zymo Shield / 20 µl DNA:60 µl Zymo Shield). O volume of 5µl of the PCR product was loaded on a gel for confirmation of the reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 % ethanol was added to the aqueous phase (1:1, v/v) and transferred to RNA Clean and Concentrator (RCC) ™ −5 kit (Zymo Research; R1013) and centrifuged at 16,000 x g for 30 s ...
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bioRxiv - Genetics 2020Quote: ... Reactions were then quenched by the addition of 1 volume DTT 1 M and then purified on an RNA Clean & Concentrator-5 column (Zymo Research, cat. R1013).
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bioRxiv - Bioengineering 2022Quote: ... DMS-modified nucleic acids were purified using a Zymo RNA Clean and Concentrator-5 Kit (Zymo Research) and eluted in 10 μl RNase-free water.
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bioRxiv - Developmental Biology 2021Quote: ... and submerged in digest solution containing 1X Digest Buffer (Zymo Research, Cat#: D3050-1-5) and supplemented with 1mg/mL Proteinase K (Zymo Research ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 aliquots were collected in 1 ml stabilization buffer tubes (DNA/RNA Shield, Zymo Research, Irvine, California) using sterile swabs ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 and 4 by lysing cells in the 6-well plate in RNA lysis buffer (Zymo) and freezing at −80 °C until extraction ...
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bioRxiv - Genomics 2024Quote: ... and 5 µL of DNA Digestion Buffer and 1 µL of DNase I (Zymo Research; R1013) were added ...
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bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
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bioRxiv - Genetics 2023Quote: ... Microinjections were performed with 4-5 µg/µl (diluted in nuclease free water, Zymo Research) fas5 and GFP dsRNA on a Femtojet microinjector (Eppendorf ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 ml Trizol (R2050-1, Zymo Research) as well as 200 µl chloroform were added to the interface and phase separation and removal were repeated ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
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bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
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bioRxiv - Bioengineering 2021Quote: 1 μg of genomic DNA was hydrolyzed overnight at 37 ºC with 5 U of DNA Degradase Plus (Zymo Research) following the manufacturer’s protocol in 50 μl volume ...
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bioRxiv - Synthetic Biology 2020Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and DNA was extracted at days 1 and 5 utilizing the Quick-DNA/RNA Miniprep (Zymo Research). Viral genomes were quantified using a standard curve and normalized to RNAseP.
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bioRxiv - Molecular Biology 2023Quote: ... PCR product sizes were verified on a 1% agarose gel prior to PCR clean up (DNA Clean & Concentrator-5, Zymo). A DNA fragment for transcription of lpp (lpp sequence preceded by aforementioned T7 transcription-enabling sequence ...
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bioRxiv - Biochemistry 2023Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Biochemistry 2024Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Bioengineering 2024Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Genomics 2020Quote: ... DNA in the initial 20-μL nucleic acids solution was digested by adding 16-μL DNase I (1 U/μL) plus 4-μL 10X DNase I buffer (DNase I set; Zymo Research), incubating for 15 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... followed by max speed centrifugation at 4 °C for 5 minutes and DNA purification by column purification (Zymo) prior to sample preparation for next-generation sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... DNase I (1 U ml−1, Zymo Research, #E1010), MgCl2 (5 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... the aqueous phase was diluted back in TRizol reagent (1:3) to proceed with RNA purification by zymodirect RNA kit (Zymo research, cat. R2051). The quality of RNA samples was assessed using Agilent 2200 TapeStation (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at 37°C for 1 hour and then purified with Zymo DNA Clean & Concentrator-5 (Zymo Research) following the PCR product protocol and eluting DNA into 10.5 μL of water ...
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bioRxiv - Biochemistry 2024Quote: ... at 37 °C for specific time (20 min, 1 hour, 2 hours, 4 hours, or 16 hours) followed by Oligo Clean & Concentrator Kits (OCC, Zymo Research, D4061) cleanup ...
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bioRxiv - Evolutionary Biology 2022Quote: ... strains were struck to synthetic defined (SD) media (CSM minus arginine, yeast nitrogen base, glucose) containing 5-Fluoroorotic Acid (5FOA) (Zymo Research, Irvine, CA, USA). to isolate cells that spontaneously lost pTC416-SEC53 ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and 3 swabs (2 for metagenomic DNA isolation, 1 for storage) moistened with DNA/RNA shield (Zymo Research, Cat. No. ZYR.R1100-250). Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e ...
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bioRxiv - Molecular Biology 2024Quote: ... Coprecipitated proteins were released from the beads by heating/denaturation (CBD-, s-AP) in 1 × loading buffer or eluting with Strep-Elution buffer (Strep-AP, Zymo Research, P2004-3-30) or lysis buffer containing 3xFlag peptide (Flag IP ...