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Citations for Zymo Research :
1 - 50 of 1427 citations for 3 4 Hydroxy 5 isopropyl 6 oxo 1 6 dihydro pyrimidin 2 ylsulfanyl propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... 3 and 4 by lysing cells in the 6-well plate in RNA lysis buffer (Zymo) and freezing at −80 °C until extraction ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl DNase I (6 U/μl) (Zymo Research) and 75 μl DNA Digestion Buffer (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... ∼4-6×109 cells were concentrated and suspended in 500 μl of DNA/RNA Shield (Zymo Research) in 2 mL screw cap tubes ...
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bioRxiv - Molecular Biology 2023Quote: ... cfRNAs were then purified and concentrated into 6 µl using an RNA Clean and Concentrator-5 kit (Zymo, R1016). Plasma extracellular vesicles (EVs ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.075% 5-fluorooritic acid (Zymo Research)) ...
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bioRxiv - Genomics 2023Quote: ... cytosines in 87bp DNA fragment were modified with hydroxy-methylation by PCR amplification with 5-hydroxymethyl-dCTP supplement (ZYMO). The hydroxy-methylated DNA fragment was combined with equal amounts of CpG methylated pUC19 DNA (NEB ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from late third instar fat bodies (n = 4-6 pooled/sample) using a Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA from 105 days old CorticOs (N = 4; n = 6-8 per genotype) was extracted using the Direct-zol RNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2024Quote: 20 zebrafish embryos per biological replicate were collected at 4 or 6 hpf and snap-frozen in 300 µl of DNA/RNA Shield (#R1100-50, Zymo Research). Total RNA then was extracted using Direct-zol RNA Miniprep Kit (#R2050 ...
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bioRxiv - Microbiology 2020Quote: ... 6) ZymoBIOMICS™ DNA Microprep kit (Zymo Research, California, USA), by the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 10 mL 5-Fluoroorotic Acid (Zymo Research), 50 mg uracil (MP Biomedicals 103204) ...
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bioRxiv - Genetics 2022Quote: RNA was extracted from pellets of ∼2 million iPSCs or ∼6 million neurons immediately after harvesting with the Quick-RNA Miniprep Kit (Zymo R1054) following manufacturer’s instructions except for two alterations ...
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bioRxiv - Developmental Biology 2024Quote: ... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... 1(10)6 lymphocytes were harvested into an equal volume of Trizol and DNA/RNA shield (Zymo Research R110-250). Total RNA was extracted using using Zymo Directzol Microprep (Zymo Research R2060 ...
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bioRxiv - Bioengineering 2021Quote: ... Selection for pyrF mutations was performed with 5-fluoroorotic acid (5-FOA, Zymo Research, part number F9001-5) at a final concentration of 0.5 mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... selective media with 5-fluoroorotic acid (Zymo Research, Tustin, CA), or YPD selective media (YPD G418+) ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from each well of a 6-well plate containing ES or DE cells using 1 mL Tripure and the Direct-Zol RNA Extraction kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... assembly products were purified and eluted in 6 μL water (Zymo Clean and Concentrate).
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bioRxiv - Molecular Biology 2023Quote: ... The products were purified and eluted in 6 μL water (Zymo Clean and Concentrate). Using the same procedure as above ...
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bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
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bioRxiv - Developmental Biology 2024Quote: ... DNA was hydrolysed for 6 h at 37°C using DNA degradase plus (Zymo Research). Mass spectrometry quantification was performed as previously described36 in the Babraham Institute mass spectrometry facility using three individual E18.5 wild-type and three individual E18.5 Tet3 knockout embryos.
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bioRxiv - Biochemistry 2023Quote: ... the products were individually purified and eluted in 6 μL water (Zymo Clean and Concentrate). NEB-10β E ...
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
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bioRxiv - Molecular Biology 2023Quote: ... The assembly products were then cleaned and eluted in 6 μL water (Zymo Clean and Concentrate). Then ...
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bioRxiv - Microbiology 2024Quote: ... added to 6 mL of DNA binding buffer from the DNA Clean & Concentrator kit (Zymo Research). DNA purified with this kit and eluted in 25 µL of DNAse/RNAse free water ...
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bioRxiv - Molecular Biology 2022Quote: ... The nucleic acid was purified using a RNA Clean & Concentrator-5 (Zymo Research) and eluted in 7µL H2O.
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bioRxiv - Developmental Biology 2022Quote: ... DNA was extracted from 6-14 dpf embryos (after yolk removal) using the DNA miniprep kit (Zymo). PCR products were amplified with Phusion (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... 75ng of DNA isolated using both lab types (n=6) and the Zymobiomics microbial community standard was used as a control (n=1) (Zymo Research Corporation, Irvine, CA, USA). Sequencing of all samples was run for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... and 3×104-5×104 cells were collected for RNA isolation (RNA Clean & Concentrator-5, Zymo # R1013). The isolated RNA was hybridized with a nCounter custom probe set (108 probes ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated at 65 °C for 6 hours and extracted with ChIP DNA Clean&Concentrator kit (Zymo Research, USA).
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bioRxiv - Pathology 2024Quote: ... Adapter-ligated fragments of 150-250bp and 250-300bp were recovered from a 2/5% NuSieve 1:1 agarose gel (Zymoclean Gel DNA Recovery Kit, Zymo Research). The fragments were then bisulfite treated using EX DNA Methylation-Lightning Kit (Zymo Research) ...
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bioRxiv - Genomics 2022Quote: ... 0.25 μL of Proteinase K (Zymo, D3001-2-5), and 2.5μL of H2O ...
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bioRxiv - Microbiology 2024Quote: ... Replicon DNA was isolated after another 6-hour growth using the ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research). Reporter replicon-transfected HEK293T cells were imaged 48 h after transfection on an EVOS M5000 Imaging System (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... lysate from ∼1×106 cells in the well of a 6-well plate was collected in 300 µL of DNA/RNA Shield (Zymo). RNA was isolated using the Quick-RNA Miniprep Plus Kit (Zymo ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then cultured for 6 days prior to RNA isolation using the ZR-Duet DNA/RNA MiniPrep Plus Kit (Zymo D7003). RNA quality and concentration were confirmed on an Agilent TapeStation and Life Technologies Qubit ...
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bioRxiv - Plant Biology 2020Quote: ... total RNA were prepared from rosettes of three biological replicates of four (Fig. 10) and five (Fig. 6) week-old plants using the Direct-zolTM RNA Mini Prep Kit (Zymo Research). DNA contaminations were removed using Turbo DNase I ...
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bioRxiv - Cell Biology 2020Quote: Organoids were washed with PBS and 6 wells/condition were pooled in 300 µL of TRI Reagent (Zymo Research, Irvine, CA) and kept at -80°C until total RNA extraction ...
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bioRxiv - Microbiology 2024Quote: ... three replicates with 6-20 guts were collected in a screw-cap tube containing 300 μl of DNA/RNA Shield (Zymo Research) (details on number of guts obtained for each sample can be found in Supplementary Table S1).
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bioRxiv - Bioengineering 2024Quote: Total RNA was extracted from SCD or HD HSPCs (15, 24, 48, 72 hours and 6 days post-transfection) using the Quick-DNA/RNA Miniprep (ZYMO Research). RNA was treated with DNase using the DNase I kit (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... DMS-modified nucleic acids were purified using a Zymo RNA Clean and Concentrator-5 Kit (Zymo Research) and eluted in 10 μl RNase-free water.
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bioRxiv - Synthetic Biology 2020Quote: ... were pretreated with a solution of β-mercaptoethanol plus 60 mM EDTA and then incubated with 6 U/mL zymolyase (Zymo Research) in Buffer I to remove the cell wall ...
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bioRxiv - Microbiology 2022Quote: ... 6-10 aphids colonized with fluorescent bacteria from each condition were collected and crushed in DNA/RNA shield (Zymo Research, CA, USA). Whole aphid RNA was purified using an RNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Cancer Biology 2023Quote: In vitro-infected keratinocytes and SCC cells were lysed in 6-wells with 1mL TRIzol Reagent and the RNA was purified by using the Direct-zol™ RNA Miniprep kit (Zymo Research). The procedure was performed according to the manufacturer protocol except for an additional 1 min centrifugation after the last washing step to completely remove eventual ethanol leftover ...
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bioRxiv - Plant Biology 2023Quote: ... Each biological replicated was generated by pooling three individual ChIP pull-downs (each consisting of 6 g of whole seedling tissues) during DNA purification using the ChIP DNA Clean & Concentrator Kit (Zymo Research, D5201). The samples were sent to BGI Genomics for library preparation and sequencing using DNBSEQ ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from three biological replicates of vehicle-treated (Veh) and 48 h-TTX-treated (TTX) neurons (6 samples in total) were isolated using Quick-RNA Microprep kit (Zymo Research, R1051) according to the instructions of manufacturer ...
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bioRxiv - Genomics 2024Quote: ... The pre-adenylated linker was eluted in 6 µl H2O following the clean-up using the Oligo Clean & Concentrator Kit (Zymo Research, D4060).
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bioRxiv - Biochemistry 2024Quote: ... at 37 °C for specific time (20 min, 1 hour, 2 hours, 4 hours, or 16 hours) followed by Oligo Clean & Concentrator Kits (OCC, Zymo Research, D4061) cleanup ...