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Citations for Zymo Research :
51 - 97 of 97 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... resulting plasmids are transfected into competent cells (Zymo Mix & Go! Competent Cells Zymo 10B #T3019), plated on a 10 cm dish and left at 37°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were propagated by transforming Escherichia Coli (Mix&Go! Competent Cells - DH5 Alpha, Zymo Research) using standard procedures ...
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bioRxiv - Plant Biology 2024Quote: ... All cloned plasmids were transformed into the Mix & Go DH5alpha competent cells (Zymo Research, USA) and selected on LB agar plates containing the relevant antibiotics ...
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bioRxiv - Microbiology 2023Quote: ... Competent Salmonella TE3006 cells were prepared using the Zymo Mix and Go kit (Zymo Research), following the provided instructions ...
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bioRxiv - Microbiology 2019Quote: ... qPCR was used to quantify 16S rRNA gene abundances [40] using the Femto™ Bacterial DNA Quantification Kit (Zymo Research). Assays were conducted following manufacturer instructions using a BioRad CFX Connect™ Real-Time PCR Detection System ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmid DNAs were transformed and amplified in Mix & Go Competent Cells-Strain HB 101 (Zymo Research ...
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bioRxiv - Synthetic Biology 2022Quote: ... All constructs were cloned into Escherichia coli TOP 10 strain using Mix&Go kit (Zymo Research) as indicated by the manufacturer ...
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bioRxiv - Genetics 2023Quote: ... The injection mix was prepared by mixing the purified plasmid DNA (Zymo D4016 PLASMID MINIPREP-CLASSIC) with DNA lad-der (1 kb Plus DNA Ladder ...
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bioRxiv - Systems Biology 2024Quote: ... and cloned these into 10beta cells made competent via the Mix-and-go kit (Zymo Research). Clones from these were arrayed in a 96-well plate and screened for chloramphenicol resistance that would indicate presence of the Gateway counterselection cassette ...
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bioRxiv - Synthetic Biology 2024Quote: ... and transformed into MegaX cells made chemically competent (Mix & Go E. Coli Transformation Kit, Zymo Research). The top 8 variants of each library selection were constructed ...
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bioRxiv - Plant Biology 2021Quote: ... The ITS regions were amplified by qPCR using ITS-specific primers provided by the Femto(tm) Fungal DNA Quantification Kit (Zymo Research) to quantify the relative abundance of fungal DNA following the manufacturer’s recommendations for the three biological replicates ...
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bioRxiv - Genomics 2021Quote: ... The libraries were pooled based on qPCR data and subjected to bisulfite conversion using the EZ DNA Methylation Direct Kit (Zymo Research) with changes to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA extracted from individual particle-attached communities was quantified through qPCR of the 16S rRNA gene using the Femto Bacterial DNA Quantification Kit (Zymo Research), which has a lower limit of detection of 20fg ...
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bioRxiv - Microbiology 2022Quote: ... or by RT-qPCR (IDT technologies, Charité kit) after RNA extraction with TRI reagent and Direct-Zol RNA miniprep kit (Zymo Research).
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bioRxiv - Microbiology 2023Quote: ... the reaction was prepared in a volume of 20 µL consisting in 2 µL of the template DNA and 18 µL of Femto Bacterial qPCR Premix (Femto Bacterial DNA Quantification Kit, Zymo Research). The PCR reaction was performed using a 7500 Fast Real-Time PCR System (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: 96-well PCR plates with 50 µL of DH10B ‘Mix & Go Competent Cells’ (prepared using Zymo T3001) in each well were prepared beforehand so that high-throughput transfers could be done using multichannel pipettes or the Tecan Fluent ...
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bioRxiv - Immunology 2022Quote: ... The assembled plasmid was directly transformed into competent SM10λpir using Mix&Go competent cells (Zymo Research Corporation) and plated on selective LB plates containing 50 μg/mL kanamycin and 15 μg/mL gentamicin ...
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bioRxiv - Immunology 2019Quote: ... The assembled plasmid was directly transformed into competent SM10λpir using Mix&Go competent cells (Zymo Research Corporation) and plated on selective LB plates containing 50 µg/mL kanamycin and 15 µg/mL gentamicin ...
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bioRxiv - Developmental Biology 2019Quote: ... The mix was then treated with a Zymo DNA Clean and Concentrator-5 Kit (Zymo research #D4014). ATAC-seq libraries were constructed in the same manner as that described above for ChIP-seq libraries.
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bioRxiv - Synthetic Biology 2020Quote: ... Frozen aliquots of these cells were prepared with the “Mix & Go!” Transformation Kit (Zymo Research, Irvine, CA). All restriction enzymes ...
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bioRxiv - Immunology 2021Quote: ... Amplicons were purified directly from PCR reaction mix by using DNA Clean & Concentrator Kits (Zymo Research, #D4013) or recovered from agarose gel by using Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Genomics 2023Quote: ... 2 µl adapter filling mixture containing 0.375 mM 5-Methylcytosine dNTP Mix (Zymo Research, cat. no. D1030), 0.75 µl of Thermopol Reaction buffer (New England Biolabs ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were suspended in 6 ml of 1:1 mix of 1 M sorbitol: YPDplus medium (Zymo Research) and incubated at 30°C for 1 h ...
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bioRxiv - Systems Biology 2024Quote: ... These pools were each transformed into competent 10beta cells prepared with a Mix and Go kit (Zymo Research), selected with LB+Carb media ...
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bioRxiv - Evolutionary Biology 2024Quote: ... pipetted the mix into a column provided in the Direct-zol RNA Miniprep Plus kit (Zymo Research, #R2072) and followed manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 75μl supernatants were collected at 48hpi and inactivated 1:1 in 1X DNA/RNA Shield for RNA extraction and RT-qPCR analysis (Zymo Research, Irvine, CA). RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected at 48hpi and inactivated 1:1 in 1X DNA/RNA Shield for RNA extraction and RT-qPCR analysis (Zymo Research, Irvine, CA). RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2021Quote: ... Complementary plasmids were co-transformed into competent MC1061ΔpspF cells carrying the reporter plasmid pLY54 (prepared using the Mix & Go E. coli Transformation Kit from Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... suspensions were transferred into ZR BashingbeadTM lysis tubes containing a mix of 0.1 mm and 0.5 mm zirconium beads (Zymo Research) and 750 μL lysis buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pQE60::trpCp variants were transformed into the ΔtrpD JW1255-1 strain (BW25113) from the KEIO collection 47 using the Mix & Go! protocol from Zymo Research (Zymo Research ...
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bioRxiv - Genomics 2022Quote: ... The reaction is incubated in the thermocycler at 50 degrees for 30 minutes and 2μL of assembled reactions were transformed into 20 μL of competent Stbl3 generated by Mix and Go! competency kit (Zymo) and plated on agar plates supplemented with appropriate antibiotics ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μL of assembled reactions were transformed into 20 μL of competent Stbl3 generated by Mix and Go! competency kit (Zymo) and plated on agar plates supplemented with appropriate antibiotics ...
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bioRxiv - Bioengineering 2021Quote: ... 2μL of assembled reactions were transformed into 20 μL of competent Stbl3 generated by Mix and Go! competency kit (Zymo) and plated on agar plates supplemented with appropriate antibiotics ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells were prepared and transformed with plasmids using the Mix and Go™ kit (Zymo Research, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Genetics 2024Quote: ... A cell line was prepared encoding this donor plasmid by transforming BL21(DE3) and making the resultant cell line chemically competent using Mix & Go preparation kit (Zymo). The temperature sensitive donor plasmid was then transformed with a second plasmid encoding a T7-inducible recombinase and a constitutively expressed bridge RNA ...
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bioRxiv - Cell Biology 2022Quote: All above constructs were transformed into Top10 competent cells prepared using Mix & Go E.coli Transformation Kit and Buffer set (Zymo Research #T3002), cultured on LB agar plates to select for antibiotic resistance using standard workflows for molecular cloning and DNA production 46 ...
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bioRxiv - Microbiology 2019Quote: ... 5 µL of ligated plasmid was then transformed into competent BW23473 cells (made using the Mix & Go E. coli Transformation Kit & Buffer Set, Zymo Research) and plated on LB + amp plates and grown overnight ...
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bioRxiv - Bioengineering 2021Quote: ... coli were made chemically competent following either the Inoue method84 or using the Zymo Research Mix & Go! Protocol (Zymo Research T3002). E ...
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bioRxiv - Immunology 2021Quote: ... 5µl of each reaction including a negative control without scFv was subsequently transformed into 100 µl Fast Transformation of Mix & Go Competent Cells (Zymo T3001) each and plated on LB plates containing Ampicillin ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μL each reaction was transformed into 100 μL DH10B and transformed according to the Mix and Go Transformation kit (Zymo Research).
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bioRxiv - Synthetic Biology 2023Quote: ... Constructs were circularized with vector backbone by Gibson assembly and introduced to XL-1 Blue Mix and Go chemical competent cells (Zymo Research), miniprepped (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... pEXG2 suicide vector containing 700-bp sequences of the flanking regions of the selected gene was directly inserted into competent SM10λpir (Mix&Go competent cells, Zymo Research Corporation) and subsequently selected on LB-Agar supplemented with 50 μg/mL kanamycin /15 μg/mL gentamicin ...
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bioRxiv - Cell Biology 2020Quote: ... mixed 1:1 with 100% ethanol and the mix was transferred to a column of the Direct-zol RNA MiniPrep Plus Kit (Zymo Research, #R2070). The following purification steps were done according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The amplicons were treated with DpnI restriction enzyme to remove the plasmid template and transformed into XL10 gold competent cells prepared by ‘Mix and Go!’ transformation kit (Zymo Research, T3001). The sequence of the isolated plasmids was confirmed using nanopore sequencing (Plasmidsaurus ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α λpir (Table 1) was prepared using the commercially available Mix and Go™ transformation kit (Zymo Research International, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...