Labshake search
Citations for Zymo Research :
1 - 50 of 1304 citations for 1 Boc 4 chloro 5 formyl 3 6 dihydro 2H pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
-
bioRxiv - Developmental Biology 2021Quote: ... 3 and 4 by lysing cells in the 6-well plate in RNA lysis buffer (Zymo) and freezing at −80 °C until extraction ...
-
bioRxiv - Microbiology 2022Quote: ... the 2h condition was harvested in TRI Reagent (Zymo Research, R2050-1) and the rest of infected-HIE were resuspended in BME and plated in a pre-warmed 24-well plate (3x 10uL drop/condition) ...
-
bioRxiv - Microbiology 2020Quote: ... 5 μl DNase I (6 U/μl) (Zymo Research) and 75 μl DNA Digestion Buffer (Zymo Research ...
-
bioRxiv - Developmental Biology 2024Quote: ... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...
-
bioRxiv - Immunology 2024Quote: ... and 3×104-5×104 cells were collected for RNA isolation (RNA Clean & Concentrator-5, Zymo # R1013). The isolated RNA was hybridized with a nCounter custom probe set (108 probes ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... ∼4-6×109 cells were concentrated and suspended in 500 μl of DNA/RNA Shield (Zymo Research) in 2 mL screw cap tubes ...
-
bioRxiv - Molecular Biology 2023Quote: ... cfRNAs were then purified and concentrated into 6 µl using an RNA Clean and Concentrator-5 kit (Zymo, R1016). Plasma extracellular vesicles (EVs ...
-
bioRxiv - Genetics 2023Quote: ... Microinjections were performed with 4-5 µg/µl (diluted in nuclease free water, Zymo Research) fas5 and GFP dsRNA on a Femtojet microinjector (Eppendorf ...
-
bioRxiv - Physiology 2020Quote: Total RNA was extracted from late third instar fat bodies (n = 4-6 pooled/sample) using a Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
-
bioRxiv - Developmental Biology 2024Quote: Total RNA from 105 days old CorticOs (N = 4; n = 6-8 per genotype) was extracted using the Direct-zol RNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocols ...
-
bioRxiv - Developmental Biology 2024Quote: 20 zebrafish embryos per biological replicate were collected at 4 or 6 hpf and snap-frozen in 300 µl of DNA/RNA Shield (#R1100-50, Zymo Research). Total RNA then was extracted using Direct-zol RNA Miniprep Kit (#R2050 ...
-
bioRxiv - Genomics 2022Quote: ... followed by max speed centrifugation at 4 °C for 5 minutes and DNA purification by column purification (Zymo) prior to sample preparation for next-generation sequencing.
-
bioRxiv - Genomics 2023Quote: ... and gDNA wash buffers from Zymo (#D3004-1, #D3004-5). DNA library preparation was also performed in 96-well plates ...
-
bioRxiv - Molecular Biology 2024Quote: ... Strep-Tactin resin (Zymo Research, catalog number P2004-1-5), or S-protein agarose (Novagen ...
-
bioRxiv - Molecular Biology 2023Quote: ... Infectious viral stocks were inactivated by a 1:3 dilution with TRI Reagent® (Zymo, R2050-1-200) immediately prior to RNA extraction ...
-
bioRxiv - Genomics 2020Quote: ... The genomic DNA of JLat cells and CD4 T cells from individuals #3 and #4 were extracted with a Quick-DNA Miniprep Plus kit (Zymo Research #D4068) according to the product protocol ...
-
bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
-
bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
-
bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
-
bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
-
bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
-
bioRxiv - Physiology 2023Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick- RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
-
bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
-
bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
-
bioRxiv - Microbiology 2022Quote: ... samples were homogenized 3:1 weight:volume in DNA/RNA Shield® (Zymo® Research Inc.).
-
bioRxiv - Cancer Biology 2020Quote: Approximately 1.4kb of genomic fragment spanning SALL4 5’ UTR - exon 1 - intron 1 was PCR amplified (Zymo Research) and cloned into the pGEM-T Easy vector ...
-
bioRxiv - Bioengineering 2024Quote: ... 1 mL of suspended biomass was collected in triplicate from the mixed tank and PBR effluent and stored in 5 mL polypropylene transport tubes (FisherbrandTM, USA) prefilled with 3 mL DNA/RNA ShieldTM (Zymo Research corp., CA, USA). The samples were briefly vortexed and stored at -20 °C until they were shipped to University at Buffalo (UB ...
-
bioRxiv - Biochemistry 2024Quote: ... The reaction mixture was incubated at 65 °C for 3 h and the RNA was then isolated by using the Zymo RNA clean and concentrator-5 kit (Zymo Research, cat. no. R1013) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2022Quote: ... at a ratio of 1:3 (DNA : Zymo Shield / 20 µl DNA:60 µl Zymo Shield). O volume of 5µl of the PCR product was loaded on a gel for confirmation of the reaction ...
-
bioRxiv - Synthetic Biology 2022Quote: ... at a ratio of 1:3 (DNA : Zymo Shield / 20 µl DNA:60 µl Zymo Shield). O volume of 5µl of the PCR product was loaded on a gel for confirmation of the reaction ...
-
bioRxiv - Microbiology 2022Quote: ... 1(10)6 lymphocytes were harvested into an equal volume of Trizol and DNA/RNA shield (Zymo Research R110-250). Total RNA was extracted using using Zymo Directzol Microprep (Zymo Research R2060 ...
-
bioRxiv - Systems Biology 2022Quote: ... the second sample was stored at RT for 3 – 5 days and the third sample was collected in a Zymo research tube (Zymo Research Inc., Irvine, CA, USA) containing 9ml DNA/RNA shield and stored at RT for 3 – 5 days before storage at −80 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... 3 aliquots were collected in 1 ml stabilization buffer tubes (DNA/RNA Shield, Zymo Research, Irvine, California) using sterile swabs ...
-
bioRxiv - Genomics 2023Quote: ... RNA was extracted from each well of a 6-well plate containing ES or DE cells using 1 mL Tripure and the Direct-Zol RNA Extraction kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... and submerged in digest solution containing 1X Digest Buffer (Zymo Research, Cat#: D3050-1-5) and supplemented with 1mg/mL Proteinase K (Zymo Research ...
-
bioRxiv - Pathology 2024Quote: ... Adapter-ligated fragments of 150-250bp and 250-300bp were recovered from a 2/5% NuSieve 1:1 agarose gel (Zymoclean Gel DNA Recovery Kit, Zymo Research). The fragments were then bisulfite treated using EX DNA Methylation-Lightning Kit (Zymo Research) ...
-
bioRxiv - Bioengineering 2021Quote: ... Selection for pyrF mutations was performed with 5-fluoroorotic acid (5-FOA, Zymo Research, part number F9001-5) at a final concentration of 0.5 mg/ml ...
-
bioRxiv - Genomics 2024Quote: ... and 5 µL of DNA Digestion Buffer and 1 µL of DNase I (Zymo Research; R1013) were added ...
-
bioRxiv - Microbiology 2020Quote: ... 6) ZymoBIOMICS™ DNA Microprep kit (Zymo Research, California, USA), by the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... 100 % ethanol was added to the aqueous phase (1:1, v/v) and transferred to RNA Clean and Concentrator (RCC) ™ −5 kit (Zymo Research; R1013) and centrifuged at 16,000 x g for 30 s ...
-
bioRxiv - Genetics 2020Quote: ... Reactions were then quenched by the addition of 1 volume DTT 1 M and then purified on an RNA Clean & Concentrator-5 column (Zymo Research, cat. R1013).
-
bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
-
bioRxiv - Plant Biology 2023Quote: ... Relative quantification of 5-methylcytosine (5-mC) over time was determined using the 5-mC DNA ELISA Kit (Zymo Research), using 100 ng of genomic DNA per sample as input ...
-
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
-
bioRxiv - Genomics 2024Quote: ... 5 µL DNA Digestion Buffer and 5 µL DNase I (Zymo Research; R1013) was added ...
-
bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
-
bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
-
bioRxiv - Microbiology 2024Quote: ... 75ng of DNA isolated using both lab types (n=6) and the Zymobiomics microbial community standard was used as a control (n=1) (Zymo Research Corporation, Irvine, CA, USA). Sequencing of all samples was run for 24 hours ...