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Citations for Zymo Research :
401 - 430 of 430 citations for hsa mir 222 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The vector and PCR products were digested with restriction enzymes and band-purified (vector) or cleaned (PCR products) using the Zymoclean Gel DNA Recovery Kit (Zymo Research) or the DNA Clean & Concentrator-5 kit (Zymo Research) ...
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bioRxiv - Genomics 2024Quote: ... DNA was eluted in 105 µL RNAse free H2O and was further cleaned using OneStep PCR Inhibitor Removal Kit (Zymo Research). Concentrations for all DNA samples were measured using Qubit 3.0 Fluorometer (Thermo Fisher Scientific ...
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In vitro impact of fluconazole on oral microbial communities, bacterial growth and biofilm formationbioRxiv - Microbiology 2023Quote: ... and quantified by real-time PCR using Zymo DNA quantification kits for bacteria/fungi (Femto Bacterial and fungal DNA Quantification kit, Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... the dsDNA target was PCR amplified from the plasmid containing the protospacer and purified by DNA Clean & Concentrator-25 (Zymo Research). The reaction was initiated with incubating 1 μM Casπ RNP effectors with 20 nM dsDNA target at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X ...
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bioRxiv - Microbiology 2023Quote: ... the same PCR fragment used for Sanger sequencing was purified using a ZymocleanTM Gel DNA Recovery Kit (Zymo Research, cat# D4002). DNA (∼1 µg ...
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bioRxiv - Synthetic Biology 2024Quote: Yeast genomic DNA was extracted from the potential aMF biosensor yeast strains and the unedited yWS677 strain (to be used as negative control in PCR analysis) using the YeaStar Genomic DNA kit and protocol (Zymo Research), with no known deviations.
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bioRxiv - Immunology 2019Quote: ... PCR products were applied to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... we added negative DNA extractions and PCR controls from each time point and mock communities (ZymoBIOMICS microbial community standard, Zymo, Irvine, CA) to each library for additional quality control and inference.
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bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: Six PCR reactions from each sample were pooled and purified with the DNA Clean & Concentrator™ kit (Zymo Research, Irvine, CA, USA), and quantified with Qubit 1.0 fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... PCR products were subjected to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The sgRNA target sites were amplified using PCR and purified using the DNA clean and concentrate kit (Zymo Research, Cat. No. D4033). The purified PCR amplicon products were sequenced by Eurofins Genomics ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were isolated from agarose gel after electrophoresis and purified using Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, CA, USA). Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR amplified DNAs were purified by DNA Clean and Concentrator or Zymoclean™ Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Primers were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were purified from agarose gels using the Zymoclean® Gel DNA Recovery Kit or from PCR reactions using the DNA Clean & Concentrator®-5 DNA kit from Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... The assembled plasmids were confirmed by standard yeast colony PCR and then extracted from yeast using the Zymoprep Yeast Plasmid Miniprep I (Zymo Research, USA). Selected plasmids were used for E ...
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bioRxiv - Microbiology 2021Quote: ... Isolated gDNAs were further prepared and cleaned up using a OneStep™ PCR Inhibitor Removal Kit according to manufacturer instructions (Zymo Research, D6030).
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bioRxiv - Cell Biology 2022Quote: IRES-Venus and human SNCA fragments were amplified by PCR (MJ Research, PTC-200 Peltier Thermal Cycler) and purified using DNA Clean & Concentrator™-5 Kit (Zymo Research, D4003) to provide 20 μl of eluted DNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 µl of the PCR product that contained the specific modules were stored in Zymo DNA/RNA shield (Zymo Research Cat.No R1100-50) in the freezer at -20 °C ...
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bioRxiv - Microbiology 2020Quote: ... the nucleic acids were resuspended in DEPC-treated nuclease-free water purified with the OneStep PCR Inhibitor Removal Kit (Zymo Research, Irvine, USA), then split into a fraction for DNA and one for RNA ...
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bioRxiv - Genetics 2020Quote: ... Two representative loci per fungal strain and the human MLH1 for the control were amplified by PCR (ZymoTaq PreMix, Zymo Research, Irvine, CA) using specifically designed primers for amplification of the bisulfite-treated DNA (designed using the ‘Bisulfite Primer Seeker’ (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from the solid and liquid aliquots using the Qiagen AllPrep PowerViral DNA/RNA kit and further purified using the Zymo OneStep PCR inhibitor removal columns (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Undigested bands corresponding to mutant PCR products were gel extracted with the Zymoclean Gel DNA Recovery Kit (Zymo Research, Cat. No./ID: D4007), then cloned into pJET vectors (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: PCR products were visualized by agarose gel (1%) electrophoresis then extracted and purified using Zymoclean Gel DNA Recovery Kit (Zymo Research Lot. #213587) following manufacturer protocols ...
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bioRxiv - Immunology 2021Quote: ... Single viable CFSElow CD3+ CD8− CD4+ T cells were sorted into 96-well PCR plates containing DNA/RNA Shield (Zymo Research, Irvine, CA, USA) using FACSAria II and Diva Software (BD Biosciences ...
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bioRxiv - Systems Biology 2022Quote: ... DNA was eluted in 60 μl elution buffer and further purified by using the OneStep PCR Inhibitor Removal Kit (ZymoBIOMICS, Zymo Research, Irvine, CA, USA).
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was prepared using the ZymoPure II Plasmid Midiprep Kit followed by an ethanol precipitation and linear expression templates were prepared by PCR and subsequent cleanup using the Zymo DNA Clean and Concentrator (Zymo Research, Irvine, CA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The barcoded PCR products were further pooled and gel-extracted using Zymo gel extraction kit and DNA clean & concentrator (Zymo research 11-301, 11-303) and quantified by Qubit 1× dsDNA HS assay kits (ThermoFisher Q32851) ...
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bioRxiv - Molecular Biology 2022Quote: ... The barcoding PCR reactions were further pooled and gel-extracted using Zymo gel extraction kit and DNA clean & concentrator (Zymo research Cat. #: 11-301 and 11-303) and quantified by Qubit 1x dsDNA HS assay kits (Thermo Fisher Scientific ...