Labshake search
Citations for Zymo Research :
3551 - 3600 of 9639 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Genomic DNA (∼500ng) was treated with sodium bisulfite using the Zymo EZ DNA Methylation-Lightning kit (Zymo Research). DNA methylation was profiled across the genome using the Illumina Infinium MethylationEPIC BeadChip (Illumina ...
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bioRxiv - Genomics 2023Quote: ... the resulting poly-A+ RNA fraction (corresponding to 0.7-2% of the starting amount) was then purified again on a Clean & Concentrator-5 Column Kit (Zymo) and eluted in 10µL of molecular-grade water.
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bioRxiv - Evolutionary Biology 2023Quote: ... we resuspended the pellet in 200 µL of nuclease-free water followed by addition of 750 µL of BashingBead buffer from the Direct-Zol RNA MicroPrep RNA kit (Zymo Research). We then transferred our 950 µL of suspension into BashingBead tubes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total RNA was purified with RNA clean and concentrator columns (Zymo Reseach, #R1013) following manufacturer instructions with critical modifications to the washing steps ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore-conjugated DNA probes were purified with Oligo Clean & Concentrator (Zymo Research D4060), and diluted to 1μM in ultrapure water ...
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bioRxiv - Genomics 2023Quote: ... Final PCR product was purified with DNA clean & concentrator (Zymo Research D4013). The primer pairs CRISPRmap-F and CRISPRmap-R in Supplementary Table 6 were used in both rounds ...
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bioRxiv - Genomics 2023Quote: ... Bacterial colonies were scraped from the agar plates and pooled together for plasmid extraction (Zymo Research D4212).
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bioRxiv - Neuroscience 2023Quote: Plasmids were digested by restriction enzymes and purified using a DNA clean & concentrator kit (Zymo research). Antisense probes were synthesized with RNA polymerases and a DIG RNA labeling mix ...
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bioRxiv - Cell Biology 2023Quote: ... The immunoprecipitated DNA were then purified using the DNA clean and concentrator 5 column (Zymo Research). The ChIP DNA was used for qPCRs using indicated primers (Table S3 ...
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bioRxiv - Genetics 2023Quote: ... Small RNAs (<200nt) size-fractionated using RNA Clean & Concentrator 5 column kits (Zymo), as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... For specific miRNA quantification, we size-fractionated small RNAs (< 200nt, containing pre-miRNAs) using RNA Clean & Concentrator 5 column kits (Zymo), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted DNA was concentrated using Genomic DNA clean and concentrator (Zymo).
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bioRxiv - Plant Biology 2023Quote: ... Secondary validation of genotyping reactions was performed as needed using the Quick-DNA Plant/Seed Miniprep kit (Zymo Research, cat#D6020). Bulk Illumina and Nanopore data from Tpd1 ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted using the Quick-DNA Miniprep Plus Kit (Zymo) according to manufacturer instruction ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from 100 µl of lysates prepared for ribosome profiling using Direct-zol mini kit (Zymo) or Trizol reagent (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The samples then were purified further using ZymoDNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA). DNA sample quality was verified by a fluorimetric Picogreen assay (>0.2 ng/µl).
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bioRxiv - Biochemistry 2023Quote: ... The extended dsDNA oligos were then purified using DNA Clean & Concentration Kits (ZYMO Research, USA). The purified and extended dsDNA were then ligated to Illumina sequencing adaptors and extended ...
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bioRxiv - Cancer Biology 2023Quote: ... we purified and concentrated both WCL_DNA and Cyto_DNA using DNA Clean & Concentrator-5 Kit (ZYMO RESEARCH; Cat #D4004) and used the DNA for quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was prepared using the Quick-RNA Miniprep Kit (R1054, Zymo, Irvine, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... but modified by inclusion of an additional RNase A treatment and a purification step using Zymo-Spin III columns (Zymo-Research). Purity of gDNA was assessed using either a Denovix DS-11 spectrophotometer or the Qubit 3 fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Colonies were collected and the plasmids were extracted with Zymoprep Yeast Plasmid Miniprep II (Zymo Research). For the next rounds of mutagenesis ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR product was run on a 1% Agarose gel and the band at the appropriate size was excised and the DNA extracted using the Zymoclean Gel DNA Recovery kit (Zymo Research). Purified insert DNA was cloned into the linearized modRNAc1 plasmid using the In-Fusion Cloning Kit (Takara Bio) ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA of the snap frozen cells was extracted using the Quick-DNA Midiprep Plus Kit (Zymo Research, D4075). sgRNA amplification was performed following a previously published protocol(49) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli JM109 (Zymo Research). The AND gate constructs were optimized by making constructs with ribosome binding site library (5’-GAAAGANNNGANNNACTA-3’ ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from cells harvested in TRIzol using Direct-zol RNA Miniprep kits (Zymo Research, R2052) with an hour DNase treatment ...
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bioRxiv - Microbiology 2023Quote: ... RNA Miniprep Plus and RNA clean-up Quick-RNA Miniprep Kits (Zymo Research) were used to isolate and purify mRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... We extracted gDNA and RNA from the tissue blocks simultaneously using the Quick-DNA/RNA Microprep Plus Kit (ZYMO Research, cat. no. D7005). We first homogenize the tissues that were stored in 1XDNA/RNA Shield following the instructions for Tough-to-Lyse samples using Biomedical FastPrep Homogenizer (MP Bio) ...
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bioRxiv - Biochemistry 2023Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted using the Zymo Direct-zol RNA Microprep Kit (Zymo Research) as per manufacturer’s instructions and stored at -80C until library preparation.
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bioRxiv - Synthetic Biology 2023Quote: ... treated with 1 μL of DpnI for 3 h at 37℃ and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo CN# D4004). Then ...
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bioRxiv - Synthetic Biology 2023Quote: DNA Clean and Concentrate kit (Zymo Research), for purifying PCR products prior to assembly (Note ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA was extracted from frozen cells using Quick-RNA Fungal/Bacterial Miniprep kit (Zymo Research, California, USA) and was reverse transcribed using a PrimeScript RT Reagent Kit (Takara ...
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bioRxiv - Microbiology 2023Quote: Isolated poly(A)+ RNA was firstly subjected to a clean-up and concentration step using the RNA clean & concentrator kit (Zymo Research). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was harvested from cells using a Quick-DNA 96 Plus Kit (Zymo Research). The DNA concentration was measured using a Qubit 1X dsDNA BR assay kit (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was prepared using the Direct-zol RNA miniprep kit (Zymo Research #R2050) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... 357-nt mini-PTC RNA constructs were purified with RNA Clean and Concentrator-25 columns (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from liver tissue was isolated using the TRIzol reagent (Direct-zol™ RNA MiniPrep, Zymo Research Corporation, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and cleaved DNA was purified using the ChIP Clean and Concentrator Kit (Zymo). Libraries were prepared with the NEBNext Ultra II Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 40–60 minutes and demethylated using rtStar tRNA-optimized First strand cDNA synthesis kit following manufacturer’s protocol with intervening RNA cleaning steps (Zymo RNA Clean and Concentration Kit). For the annealing reaction ...
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bioRxiv - Synthetic Biology 2023Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... Sequence confirmed plasmids were purified (Zyppy Plasmid Miniprep Kit, Zymo Research, Irvine, CA, Cat#D4019) for further studies.
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bioRxiv - Synthetic Biology 2023Quote: ... All plasmids were isolated from cells via miniprep (Zymo kit).
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bioRxiv - Synthetic Biology 2023Quote: ... Total RNA was extracted using a Direct-zol RNA Microprep Kit (Zymo Research) and 1,000 ng was used as input for reverse transcription ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was harvested from each sorted cell population using a Quick-DNA 96 Plus Kit (Zymo Research). The DNA concentration of each sample was measured using a Qubit 1X dsDNA BR assay kit (ThermoFisher).
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bioRxiv - Genetics 2023Quote: ... Plasmids were then transformed into Zymo Mix & Go DH5α Competent Cells (Zymo T3007) for verification and amplification before re-purification and transformation into MG1655 E ...
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bioRxiv - Genetics 2023Quote: For transformation into Zymo Mix & Go DH5α competent cells (Zymo T3007), 100 µL of cells were thawed on ice per reaction ...
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bioRxiv - Cell Biology 2023Quote: ... Bisulfite treatment was performed on 500 ng of DNA using the EZ DNA Methylation-Lightning Kit (Zymo research). Bisulfite-converted DNA was amplified using the oligonucleotides #2479 and #2480 (Supplemental Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... all 384 wells were pooled and purified with two DCC-5 columns (Zymo Research). Libraries were sequenced on a NovaSeq6000 system (Illumina ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from 200-250µm-thick cryopreserved rat lung tissue using the Zymo Quick-DNA MicroPrep Kit (Zymo, Irvine, CA), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... followed by on column cleanup with DNAse digestion (Zymo). RNA libraries were prepared with Illumina Truseq stranded mRNA kit (Illumina) ...