Labshake search
Citations for Zymo Research :
251 - 300 of 359 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and depleted samples using the Zymo Direct-zol RNA Microprep Kit with on-column DNase treatment (Zymo Research). The step-by-step Bb enrichment protocol is available at ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNase treated samples were purified further using the RNA Clean and Concentrator Kit (Zymo Research, R1015/R1017) and stored at – 80°C after addition of ribonuclease inhibitor (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... the PCR master mix has been treated with 0.02% (v/v) of DNAse I 1 U/mL (Zymo) at room temperature for 15 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The solutions were treated twice with DNAse I and thereafter purified using RNA Clean/Concentrator 25 columns (Zymo).
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted with Direct-zol RNA miniprep kit with on-column DNAse I digestion (Zymo Research). 0.5 to 1 μg of total RNA was used for reverse-transcription with ReverTra Ace qPCR RT Master Mix (Toyobo ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then purified using the Zymo RNA Clean & Concentrator kit along with DNase treatment (Zymo Research). Libraries were prepared with the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB ...
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bioRxiv - Genomics 2024Quote: ... RNA concentration step and an additional DNase treatment were undertaken using an RNA clean & concentrator kit (Zymo Research). RNA amount and integrity were checked using TapeStation 4200 (Agilent) ...
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bioRxiv - Immunology 2024Quote: ... and RCC model DNAse removal and RNA concentration were performed using a commercial kit (Zymo Research, cat. R1013) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Lysed samples were purified and DNAse treated using the Direct-zol RNA MicroPrep kit (Zymo Research Corporation, R2060). cDNA was synthesized by adding 2μg of total DNAse-treated RNA to 2μl of 100μM oligo-dT(15-18 ...
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bioRxiv - Genomics 2021Quote: ... DNA was then eluted in 20 μl deionized water using the Zymo DNA Clean and Concentrator kit (Zymo, D4033). 10 μl of eluted DNA was used for library preparation exactly as described for CUT&RUN ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was then eluted in 20 µl deionized water using the Zymo DNA Clean and Concentrator kit (Zymo, D4033). 10 µl of eluted DNA was used for library preparation exactly as described for CUT&RUN ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL ...
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bioRxiv - Molecular Biology 2023Quote: ... Added water to volume of 50 μL and cleaned up the RNA (Zymo RNA clean and concentrator -5 # R1013) and eluted in 15 μL water.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with RNase for 1 hour and proteinase K for 1 hour before ChIP DNA Clean & Concentrator (Zymo Research) to extract the DNA.
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bioRxiv - Cell Biology 2023Quote: ... The RNAse treatments were carried out for 20 min at 30°C followed by RNA extraction using DirectZolTM (Zymo Research®) kits as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× RNase H buffer overnight at 37 °C before immunoprecipitation and recovered using the Oligo Clean & Concentrator kit (Zymo Research). For m1A-DIP ...
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bioRxiv - Bioengineering 2023Quote: ... but modified by inclusion of an additional RNase A treatment and a purification step using Zymo-Spin III columns (Zymo-Research). Purity of gDNA was assessed using either a Denovix DS-11 spectrophotometer or the Qubit 3 fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by RNA purification and DNase I treatment by means of a Direct-zol™ kit (Zymo Research, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were subsequently treated with DNase I and purified using the RNA Clean and Concentrator kit (Zymo Research R1013). cDNA was synthesized using the iScript™ cDNA Synthesis Kit (1708891 ...
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bioRxiv - Microbiology 2021Quote: ... RNA was depleted by using Turbo DNase according to the manufacturer’s instructions (AM2238, Invitrogen. The DNase-treated RNA was then cleaned using an RNA clean up kit (R1019, Zymo) according to manufacturer’s instructions and DNA depletion was verified by amplifying 1 μL of RNA in a PCR reaction ...
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bioRxiv - Genetics 2022Quote: ... Samples were then spun through a binding column and on-column DNA digest was performed with DNase I (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA was cleaned and purified using an RNA Clean and Concentrator Kit with in-column DNase treatment (Zymo Research). RNA quality and quantity was quantified using a NanoDrop 2000c (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The resulting supernatant was purified using the Clean & Concentrator kit incorporating the on-column DNase digestion step (Zymo Research). Yields were determined using a Qubit 2.0 fluorometer with the RNA BR assay kit (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction and DNase treatment were performed using a Direct-zolTM RNA MiniPrep Kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted with Direct-zol RNA miniprep kit with on-column DNase I digestion (Zymo Research #R2053). 0.5 to 1 μg of total RNA was used for reverse-transcription with ReverTra Ace qPCR RT master mix (Toyobo #FSQ-101 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA was treated with DNase I and cleaned using an RNA Clean and Concentrator Kit (Zymo Research, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... DNase I treated RNA samples were purified and further concentrated using RNA clean and concentrator-5 (Zymo Research, R1014) according to the manufacture’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Omega Bio-tek®) and subjected to DNase treatment and purification by the RNA Clean & Concentrator kit (Zymo Research) to yield high-quality RNA for library preparation ...
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bioRxiv - Developmental Biology 2024Quote: ... Polysome-bound RNA was retrieved from Dynabeads and DNase-treated using the Direct-zolTM RNA MicroPrep kit (Zymo Research).
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bioRxiv - Molecular Biology 2024Quote: ... A wash using RNA wash buffer was done prior to a DNAse treatment following manufacturer recommendations (Zymo Research, E1010). After DNAse treatment ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples treated with DNAse I were subjected to purification using the RNA Clean and ConcentratorTM kit (Zymo research) following the protocol provided by the manufacturer for purification and concentration of total RNA including small RNAs ...
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bioRxiv - Neuroscience 2024Quote: ... brains were transferred to tubes containing digestion buffer (0.05% Collagenase D Sigma-Aldrich Cat. C0130-500MG; 0.1µM TLCK, Sigma-Aldrich Cat. T7254-100MG, 10µg/mL DNAse 1, Zymo Cat ...
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bioRxiv - Genetics 2020Quote: ... DNA extractions by miniprep were done with the ZymoPURE Plasmid Miniprep kit and elution with water (Zymo Research, cat. D4208T).
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bioRxiv - Molecular Biology 2023Quote: ... eluted RNA is spun out into fresh tubes and a second elution with water is performed (Zymo-I = 30μL, Zymo-II = 50μL, Zymo-IIICG = 60μL). To the eluate ...
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bioRxiv - Genetics 2020Quote: ... 100 mg of thawed cell pellets were washed three times in 1 mL deionized water and extracted using Quick-DNA Fungal/Bacterial Miniprep kit (Zymo Research). A total of 2.4 μg of purified gDNA was fragmented by sonication to average size of ~350 bp in four separate aliquots using a Diagenode Picoruptor ...
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bioRxiv - Microbiology 2023Quote: ... one for each extraction batch) using commercially available kits optimized for water and tissue-based microbial communities (Quick DNA Fecal/Soil Kit, Zymo Research). DNA extract was roughly quantified and purity-checked with a Thermo Scientific™ NanoDrop™ OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific ...
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bioRxiv - Physiology 2024Quote: ... was diluted in water and added directly to the column (50 μL for Zymo-II and 60 μL for Zymo-IICG). The column was sealed with a cap or parafilm and then incubated at 37°C for 45 min ...
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bioRxiv - Physiology 2024Quote: ... was diluted in water and added directly to the column (50 μL for Zymo-II and 60 μL for Zymo-IICG). The column was sealed with a cap or parafilm and then incubated at 37°C for 45 min ...
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bioRxiv - Microbiology 2024Quote: ... Negative controls (sterile water treated as a sample) and one positive control (ZymoBIOMICS Microbial Community Standard, Zymo Research, Cat No. D6300) were included in each batch ...
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bioRxiv - Immunology 2021Quote: ... followed by a 15 minute treatment with DNase and subsequent purification with Direct-zol™ RNA MiniPrep Plus (Zymo Research). All shRNAs used in this study can be found in Supplementary Dataset 2.
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bioRxiv - Plant Biology 2021Quote: ... RNA samples were digested with DNase I and purified using RNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR ...
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bioRxiv - Plant Biology 2020Quote: ... RNA isolation and DNase treatment was carried out by Direct-zol RNA MiniPrep Plus Kit using the manufacturer’s protocol (Zymo Research). Q-PCR was performed on a Bio-Rad IQ5 real-time PCR detection system by using iTaq Universal SYBR Green One-Step RT-qPCR kit (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from seedlings using a Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research). cDNA synthesis was performed with 2 µg of total RNA using oligo(dT ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... RNA was extracted and purified using the Direct-zol RNA miniprep kit with in-column DNAse treatment (Zymo Research, #R2051). Purified RNA was eluted in UltraPure water and stored at −80°C until needed for rt-qPCR or RNAseq library preparation ...
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bioRxiv - Systems Biology 2022Quote: ... and RNA was extracted using Direct-zol RNA mini-prep with an additional on-column DNAse digestion step (Zymo Research). The quality of the purified RNA was assessed with a Nanodrop ND-1000 spectrophotometer measuring the A260/A280 and A260/A230 ratios ...
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bioRxiv - Zoology 2020Quote: ... RNA purification and DNAse treatment were conducted following the Direct-zolTM RNA MiniPrep Kit protocol (Zymo Research, Irvine, CA, USA) with slight modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... and RNA extracted using Direct-zol columns with a 30 minute on-column DNase step at room temperature (Zymo Research). cDNA was generated with Superscript III First-Strand Synthesis SuperMix (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted using the Quick RNA Fungal/Bacterial Miniprep kit including DNase I column treatment (cat#R2014, Zymo Research). RNA was eluted in 30μL of molecular grade water and assessed for concentration and quality via Nanodrop and RNA gel electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... The cells debris was removed after centrifugation at 15000g for 5 mins and cell supernatant containing the released virions were subjected to 5μl DNase I (Zymo research) for 1 hour to remove remaining untransfected chimeric NL plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... when RNA was further purified using the RNA Clean & Concentrator kit with in-column DNase-I treatment (Zymo Research Corp), following manufacturer instructions ...