Labshake search
Citations for Zymo Research :
201 - 250 of 254 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... All the steps from here were optimized from the standard protocol provided with the EZ Nucleosomal DNA Prep Kit (D5220, Zymo Research). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA sequencing libraries were prepared by amplifying and barcoding the V1-V2 region of the 16S rRNA gene using the Quick-16S NGS Library Prep Kit (Zymo Research). Samples were pooled and sequenced on the Illumina MiSeq platform with 2 × 250 base pair standard run at Washington University DNA Sequencing Innovation Lab.
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bioRxiv - Immunology 2020Quote: ... the DNA was subjected to bisulfite conversion and PCR-based sequencing adapter tagging as described for WGBS above (Pico Methyl-Seq Library Prep Kit, Zymo Research). The library was quantified and sequenced on an Illumina Nextseq 550 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The NEBNext® Ultra™ II DNA Library Prep Kit for Illumina was used for library preparation and was combined with EZ-96 DNA Methylation-Gold™ MagPrep (ZYMO) for bisulfite libraries ...
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bioRxiv - Microbiology 2022Quote: ... DNA and RNA were extracted using the Quick DNA/RNA mini prep plus kit with ZR BashingBeads (0.1 and 0.5mm; Zymo Research, Carlsbad, CA). For coral samples ...
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bioRxiv - Plant Biology 2020Quote: ... total RNA were prepared from rosettes of three biological replicates of four (Fig. 10) and five (Fig. 6) week-old plants using the Direct-zolTM RNA Mini Prep Kit (Zymo Research). DNA contaminations were removed using Turbo DNase I ...
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bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged 1 min at 12000 g at 4°C and RNAs were extracted from supernatants with Direct-zol RNA Mini Prep kit (Zymo Research), following manufacturer’s protocol and including the optional DNAse treatment step ...
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bioRxiv - Genetics 2020Quote: ... We then prepared whole-genome bisulphite sequencing libraries from each DNA sample using the Pico Methyl-Seq Library Prep Kit (Zymo Research), according to the manufacturer’s protocol (see Supplementary Table 1 for detailed sample metadata and sequence accession codes) ...
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bioRxiv - Genomics 2022Quote: ... was added to extract total RNA using the Direct-zol RNA Mini Prep Plus kit (Zymo Research, Irvine, CA, United States). The integrity and quality of extracted RNA were checked using an Experion electrophoresis system (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... FFPE scrolls were processed for DNA and RNA extraction using a Quick-DNA/RNA FFPE Miniprep Kit with on-column DNase digestion for the RNA preps (Cat. # R1009; Zymo Research). RNA was assessed for mass concentration using the Qubit RNA Broad Range Assay Kit (Cat ...
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bioRxiv - Genomics 2023Quote: ... using an in-house Tn5 transposase (IMBA-IMP-GMI Molecular Biology Services) and an EZ-96 DNA Methylation-Gold Mag Prep kit (Zymo Research) for bisulfite conversion ...
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bioRxiv - Microbiology 2024Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Genomics 2024Quote: Genomic DNA from placental tissues was isolated using a Quick-DNA mini prep plus kit according to the manufacturer’s instructions (Zymo Research D4068). LINE-1 methylation levels were quantified using an ELISA-based Global DNA Methylation Assay LINE-1 kit ...
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bioRxiv - Developmental Biology 2024Quote: We isolated genomic DNA from all samples using the Zymo Quick DNA mini-prep Plus Kit (Zymo Research; Cat. no. D4068). The samples were amplified and prepared for sequencing as described previously (Gilbert et al ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was extracted following the CTAB method (Doyle and Doyle 1987) and ZYMO kits (ZR Plant/Seed DNA mini-Prep kit; Zymo Research, USA). The quality and quantity of DNA extracted was evaluated using spectrophotometric (NanoDrop™ 8000 ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene using primers custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from PPN was extracted using the TransZol reagent (TransGen Biotech, China) in combination with the Direct-Zol RNA mini prep kit (Zymo Research, USA). cDNA was produced from 500 ng of total RNA with EasyScript Reverse Transcriptase (TransGen Biotech Cat #AE101 ...
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bioRxiv - Microbiology 2021Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Bacterial 16S primers amplify the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Bioengineering 2020Quote: ... Single-fly genomic DNA preps were prepared using the solid tissue protocol of the Quick-DNA™ Miniprep Plus Kit (Zymo Research). In total ...
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bioRxiv - Cell Biology 2022Quote: ... ethanol was added in a ratio of 1:1 to the supernatant from the previous step and continued according to the manufacturer instructions of Direct-zol mini (for tissues) and micro (for fecal wash) prep kit (ZYMO research, R2052).
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bioRxiv - Systems Biology 2022Quote: ... the cells were lysed in 700uL of Qiazol and RNA was isolated using the Zymo Direct-zol Mini-prep kit and reagents (Zymo-Catalog # RD2051). RNA integrity was determined with a Bioanalyzer and only RNA samples with a RNA Integrity Number (RIN ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from snap-frozen cell pellets using the Quick-RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... was added to each well followed by isolation of total RNA using Direct-Zol RNA Mini-Prep Plus Kit (Zymo Research, R1058) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Vendor-designed primers for bacterial 16S amplified the V3-V4 region ...
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bioRxiv - Physiology 2024Quote: Simultaneous isolation of genomic DNA and total RNA from liver tissues was performed using the ZR-Duet DNA/RNA Mini Prep kit (Zymo Research, California). RNA was treated with DNase during the isolation process ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from ten 10 μm-thick FFPE sections using the Quick-RNA Mini Prep Plus Kit (Zymo Research, cat# R1057) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The DNA samples were prepared for targeted sequencing with the Quick-16S NGS library Prep kit and 16S primer set V3-V4 (Zymo Research Irvine, CA). The final library was sequenced on Ilumina MiSeq with a V3 reagent Kit (600 cycles) ...
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bioRxiv - Microbiology 2022Quote: ... samples were processed for RNA extraction and purification using the Zymo Direct-zol™ RNA Mini Prep kit (Zymo Research, Irvine, CA, USA) per manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... The cell pellets were washed with PBS before total RNA was extracted with the Direct-zol RNA Micro-Prep method (Cat. R2063-A, Zymo Research Corp., CA), and the extracted RNA was reverse transcribed using LunaScript® RT SuperMix Kit (Cat ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was performed according to manufacturer’s protocol using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA, USA, #D6400). The pooled libraries were sequenced on an Illumina MiSeq using MiSeq®Reagent Kit v2 (500-cycle ...
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bioRxiv - Microbiology 2024Quote: Libraries for mRNA sequencing were constructed from total RNA sample using the Zymo-Seq RiboFree Total RNA Library Prep Kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The resulting pellet was resuspended in 100 uL RNAse free water and cleaned using the Zymo Research Quick-RNA Mini-Prep Kit and DNase1 treatment (Cat # R1054, Zymo Research Corporation, Irvine, CA) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... To characterize the microbial diversity based on 16S rRNA gene sequences we used the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine CA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Large-scale DNA plasmid was amplified in Escherichia coli DH5α and purified by using a plasmid giga prep kit (Zymo Research, Costa Mesa, CA). DNA sequencing was performed to confirm the plasmid sequence’s authenticity.
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted directly from the TRI reagent using a Direct-Zol RNA Mini-Prep Kit according to manufacturer instructions (Zymo Research Corporation, Irvine, CA). RNA from all four groups was processed using a Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from the NRVMs four days after reverse transfection of siRNA using the Direct-zol RNA mini prep kit (Zymo Research, Catalog No. R2050). RNA was reverse-transcribed into complementary DNA using the qScript cDNA synthesis kit (Quanta Biosciences ...
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bioRxiv - Microbiology 2023Quote: RNA was isolated from mid-log phase protists grown at normal growth temperature or 12°C using the Direct-zol RNA Miniprep kit (Zymo). RNA libraries were generated using the KAPA Stranded mRNA Sequencing Kit (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... The 60 samples were pooled into 5 groups of 12 samples each during purification using RNA Clean & Concentrator (Zymo Research) and all eluted with 16µl DEPC UltraPure water.
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from the 12-day old plant seedlings using Direct-zol RNA Miniprep kit (Zymo research, R2050). Library preparation was performed using Nanopore Direct RNA Sequencing Kit (SQK-RNA004 ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA from either CD34+ cells sorted after transduction or bulk cells at day 12 of culture was extracted using Direct-Zol RNA MicroPrep Kit (Zymo Research) and reverse transcription was performed with SuperScript Vilo cDNA Synthesis Kit (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted (12 biological samples in total) with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... were added to media-removed cell monolayers per single well of 12 well plates followed by on-column DNA digestion and purification (Zymo Research). For RNA purification from RNP capture sample ...
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bioRxiv - Genetics 2022Quote: ... The resulting RNA product was run on 12% SequaGel -UreaGel (national diagnostics) and purified with ZR small-RNA PAGE Recovery KitTM (R1070, ZYMO RESEARCH) following provider’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... nojo-1 and nojo-2 complete roots of plants growing 12 DAS using Quick-RNA Miniprep (Zymo Research, Irvine, CA, USA). Complementary DNA was generated using the SuperScriptTMII Reverse Transcriptase (InvitrogenTM ...
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bioRxiv - Genetics 2024Quote: ... recovered for 1 hour at 30°C with shaking and then grown for 12-16 hours at 30°C prior to plasmid preparation using ZymoPURE II kits (Zymo Research). To generate the final homology-directed repair template (HDRT ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was extracted from 12-day old gametophytes using Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, Irvine, CA, USA). Primers were designed using SnapGene with elongation factor 1 (Ceric.09G081400 ...
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bioRxiv - Zoology 2021Quote: ... The supernatant was collected and added to 700 μL of 95% ethanol for total RNA recovery using the Direct-zol™ RNA mini-prep kit (Zymo Research®, Irvine, CA, USA). The solution was transferred to filter columns and centrifuged (16,000 g × 30 s) ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was reverse-crosslinked overnight (12-16 hours) at 65°C prior to ChIP DNA isolation using Zymogen ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205). For ChIP-seq ...
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bioRxiv - Neuroscience 2023Quote: Primary cortical neurons were infected at 3 DIV with FL1 and at 10-12 DIV neurons were harvested and DNA extracted using Quick-DNA Miniprep Kit (Zymo Research, USA) following manufacturer recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... the beads were boiled in Tris-EDTA containing 1% SDS and 12% β-Mercaptoethanol before RNA was extracted using a Direct-zol RNA Microprep kit (Zymo Research). The extracted RNA was then prepared for RT- qPCR analysis to examine RB1 ...