Labshake search
Citations for Zymo Research :
201 - 250 of 264 citations for Rabbit Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: ... A 2-cm-long piece of each cotton strip was cut off and transferred into a ZR BashingBead Lysis Tube (0.1 & 0.5 mm) pre-filled with 1 mL of DNA/RNAShield (Zymo Research, Freiburg, Germany) using sterile laboratory gloves ...
-
bioRxiv - Microbiology 2022Quote: ~1 kb products from the Pan-Entamoeba PCR above were gel purified using a Zymoclean Gel DNA Recovery Kit (Zymo Research) and submitted to the UW-Madison Biotechnology Center for Sanger Sequencing using the amplification primers described above ...
-
bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
-
bioRxiv - Microbiology 2023Quote: Cells were infected with 1 MOI of TB40/EGFP and DNA and RNA was isolated using Quick-DNA/RNA miniprep kit (Zymo Research) from 0–72 hpi ...
-
bioRxiv - Genomics 2023Quote: Total RNA (∼1 µg) was used to make RNA-seq libraries following the Zymo-Seq Ribo-free Total RNA Library Kit (Zymo Research) instructions with dual indexing ...
-
bioRxiv - Molecular Biology 2023Quote: ... nojo-1 and nojo-2 complete roots of plants growing 12 DAS using Quick-RNA Miniprep (Zymo Research, Irvine, CA, USA). Complementary DNA was generated using the SuperScriptTMII Reverse Transcriptase (InvitrogenTM ...
-
bioRxiv - Bioengineering 2023Quote: The 2 μL of EVs were incubated at room temperature for 15 min with 1 U of DNase I (Zymo Research) and 1x DNA Digestion Buffer ...
-
bioRxiv - Genomics 2023Quote: ... RNA was extracted from each well of a 6-well plate containing ES or DE cells using 1 mL Tripure and the Direct-Zol RNA Extraction kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Constructs were circularized with vector backbone by Gibson assembly and introduced to XL-1 Blue Mix and Go chemical competent cells (Zymo Research), miniprepped (Qiagen) ...
-
bioRxiv - Bioengineering 2023Quote: ... The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research) supplemented with RNase A (Sigma-Aldrich ...
-
bioRxiv - Genomics 2023Quote: gDNA was isolated from ∼25 mg of frozen tissue or ∼1 x 106 HepG2 cells suspended in 200 µL of PBS using the Quick-DNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocol with an overnight (for frozen tissue ...
-
bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at 37°C for 1 hour and then purified with Zymo DNA Clean & Concentrator-5 (Zymo Research) following the PCR product protocol and eluting DNA into 10.5 μL of water ...
-
bioRxiv - Immunology 2023Quote: BMDMs (1 × 106) were treated as indicated and RNA was purified using Direct-zol RNA Miniprep (Zymo Research Corporation, Cat# R2053). Samples were submitted to Azenta Life Sciences for standard RNA-seq processing ...
-
bioRxiv - Cancer Biology 2023Quote: ... Modifications to the published method are the following: 1) extraction of RNA prior to cDNA reverse transcription with the Zymo Direct-zol-96 RNA isolation kit (Zymo, #R0256) 2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... aqueous phase containing cfRNAs was mixed with 1 volume of ethanol (95-100%) and then added to the Zymo-Spin column (Zymo, R1016) for RNA binding ...
-
bioRxiv - Genomics 2023Quote: ... the supernatant was transferred to a deep 96-well plate and mixed with 400ul of genomic lysis buffer (Zymo, #D3004-1). The mix was stored at -20C to await further processing ...
-
bioRxiv - Genetics 2023Quote: ... followed by resuspension of ~200k to 1 million cells in 300ul RNA Lysis Buffer from the Quick-RNA Miniprep Kit (Zymo R1055) and stored in −70deg ...
-
bioRxiv - Molecular Biology 2024Quote: ... in 1× RNase H buffer overnight at 37 °C before immunoprecipitation and recovered using the Oligo Clean & Concentrator kit (Zymo Research). For m1A-DIP ...
-
bioRxiv - Bioengineering 2024Quote: Up to 1 × 108 yeast cells from a growing culture were taken to perform a zymoprep using the ZymoprepII kit (D2004, Zymo Research). The TRACeR sequences were amplified from the extracted plasmids and the adapter sequences were added by PCR ...
-
bioRxiv - Bioengineering 2024Quote: The 10 μL of eEVs were incubated at room temperature for 15 min with 1 U of DNase I (Zymo Research) and DNA Digestion Buffer ...
-
bioRxiv - Genetics 2024Quote: ... at 37°C for 1 hr and purified again by illustra microspin G-50 column or Direct-zol RNA Miniprep kits (Zymo Research) before transfection (Supplemental Fig ...
-
bioRxiv - Genetics 2024Quote: ... DNA from a minimum of 1 e-6 cells from each fraction was isolated using the YeaStar Genomic DNA Kit (Zymo Research). DNA was fragmented by sonication and sequenced.
-
bioRxiv - Genetics 2021Quote: DNA bisulfite conversion was performed directly starting from cell pellets (a maximum of 1×105 cells per sample) using the EZ DNA Methylation-Direct kit (Zymo Research #D5021) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... PCR products were applied to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: ... The corresponding amount of polyA RNA were incubated with 1X fragmentation buffer at 95 °C for 1 minute to generate ~300 nt of RNA fragment followed by column purification (Zymo Research, R1016) as described before [23] ...
-
bioRxiv - Molecular Biology 2021Quote: ... templates were run on a 1% agarose gel to confirm cutting and the reactions were purified with Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). 1ug of linearized template was used in a 100uL T7 RNA Polymerase (purified in house ...
-
bioRxiv - Molecular Biology 2021Quote: ... PCR products were run on a 1% agarose gel to check the product size and purified using Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). To create the cloning insert ...
-
bioRxiv - Genomics 2019Quote: ... or by sodium bisulfite using two commercially available kits: BS (1)EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA Research) and BS (2 ...
-
bioRxiv - Neuroscience 2020Quote: RNA was isolated from three biological replicates of E/I networks composed of Ngn2 #2 and Ascl1 #1 (DIV 49) with the Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: Total RNA from adipose tissues (experiment 1) was extracted using the Direct-zol™ RNA MiniPrep Plus kit protocol (Zymo research, #R2071). RNA was reverse-transcribed using the SuperScript™ III Reverse Transcriptase Kit (ThermoFisher ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
-
bioRxiv - Genetics 2020Quote: ... DNA was digested in columns by adding a mixture of 5 μl RNA Lysis Buffer 1 from the kit andl DNase I and 75 μl RNA Lysis Buffer 1 from the kit andl DNA Digestion Buffer from DNase I Set kit (Zymo Research E1010) followed by a 15-minute incubation at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Genomic DNA (1 μg) was treated with sodium bisulfite using the EZ DNA Methylation Gold Kit per manufacturer’s instructions (Zymo Research; Irvina, CA). Bisulfite converted DNA (∼20 ng ...
-
bioRxiv - Immunology 2021Quote: ... PCR products were subjected to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2022Quote: DNA bisulfite conversion was performed starting from a maximum of 1 × 105 pelleted cells per sample using the EZ DNA Methylation-Direct kit (Zymo Research #D5021), and following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Single colonies were grown in Sc-Ura medium for 1 d and plasmid DNA was purified using Zymoprep II Yeast plasmid Miniprep kit (Zymo Research, CA). Plasmids were then transformed into E ...
-
bioRxiv - Plant Biology 2023Quote: ... The adapter-ligated DNA molecules were purified using 1× Agencourt AMPure XP magnetic beads and were subjected to bisulfite conversion using ZYMO EZ DNA Methylation-Gold Kit (Zymo, Cat. #D5005). Libraries were then amplified by PCR using 20 μL of bisulfite-converted DNA molecules ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
-
bioRxiv - Microbiology 2024Quote: ... The amplified promoter and coding PIGJ DNA sequence were first purified from 1% agarose gels using the ZymocleanTM Gel DNA recovery kit (Zymo Research, #D4007) and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB ...
-
bioRxiv - Immunology 2021Quote: MSP1-specific B cells that successfully expanded in culture were collected by centrifugation (5 min. at 250 × g and RT) and stored at -70°C in 50 µl Tri-Reagent (Zymo #R2050-1-200). Heavy and light chain variable regions were amplified from MSP1-specific B cells by cDNA synthesis and a series of PCR reactions ...
-
bioRxiv - Biochemistry 2021Quote: ... Cleared supernatant was discarded and the concentrated high-speed pellet (“100,000 x g pellet”) was lysed directly in 300 μl of TRI reagent (Zymo Research, R2050-1-200). RNA extraction was performed according to instructions for the Direct-zol RNA Miniprep Kit (Zymo Research ...
-
bioRxiv - Immunology 2021Quote: ... mice were euthanized with isoflurane overdose and one lobe of lung tissue was collected in an Omni Bead ruptor tube filled with Tri Reagent (Zymo, #R2050-1-200). Tissue was homogenized using an Omni Bead Ruptor 24 (5.15 ms ...
-
bioRxiv - Molecular Biology 2022Quote: ... the aqueous phase was diluted back in TRizol reagent (1:3) to proceed with RNA purification by zymodirect RNA kit (Zymo research, cat. R2051). The quality of RNA samples was assessed using Agilent 2200 TapeStation (Agilent Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... 2 extracted with tissue samples and 1 run with water samples) and 2 samples of DNA from mock bacterial communities (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Irvine CA) were prepared and sequenced ...
-
bioRxiv - Microbiology 2022Quote: ... PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research, Irvine, CA). The purified DNA fragments were then submitted for Sanger sequencing (GENEWIZ ...
-
bioRxiv - Plant Biology 2022Quote: ... Fragments were resolved with gel electrophoresis (1% agarose in 1x TAE buffer) and excised using the Zymoclean Gel DNA Recovery Kit (Zymo Research; Ref.: D4008) for elution of high-concentration ultra-pure DNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... Two-third of final flag elute was taken for DNase treatment using NEB DNase 1 (M0303) and RNA was purified using RNA Clean and Concentrator Kit (Zymo Research, R1015/R1017). The final RNA concentration was determined using Qubit RNA high Sensitivity Assay Kit and 100ng of RIP-purified RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB ...
-
bioRxiv - Cancer Biology 2022Quote: ... The outer PCR products amplified using primer #1 and #2 were purified and concentrated using the DNA Clean and Concentrator 5 kit (Zymo Research, Orange, CA) and eluted in 20ul of TE buffer ...
-
bioRxiv - Evolutionary Biology 2023Quote: PCR products were visualized by agarose gel (1%) electrophoresis then extracted and purified using Zymoclean Gel DNA Recovery Kit (Zymo Research Lot. #213587) following manufacturer protocols ...
-
bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α λpir (Table 1) was prepared using the commercially available Mix and Go™ transformation kit (Zymo Research International, Irvin, CA, USA) according to the manufacturer’s indications ...