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Citations for Zymo Research :
151 - 200 of 1084 citations for R 4 Benzyl 2 2 diphenylphosphino benzyl 4 5 dihydrooxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... before being spun down (10k rpm, 2 minutes) and resuspended in 80 μL of digestion buffer (included in the YeaStar RNA Kit, Zymo Research). Cells were then stored on ice until ready for RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... nojo-1 and nojo-2 complete roots of plants growing 12 DAS using Quick-RNA Miniprep (Zymo Research, Irvine, CA, USA). Complementary DNA was generated using the SuperScriptTMII Reverse Transcriptase (InvitrogenTM ...
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bioRxiv - Systems Biology 2023Quote: ... Pools 4-8 from experimental replicate #2 were resuspended in 100 µL spheroplast digest buffer with 2.5U/mL Zymolyase T100 (Zymo Research #E1005) and incubated at 37°C for 20 minutes ...
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bioRxiv - Genomics 2024Quote: ... for 2 hr at 37 C and then cleaned and concentrated using a Zymo DNA Clean and Concentrator column (Zymo D4013). The debranched RCA product was run on a 1% low-melt agarose gel ...
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bioRxiv - Neuroscience 2020Quote: ... and sorted into 384-well plates preloaded with 2μl of digestion buffer for snmC-seq215 (20 mL digestion buffer consists of 10 mL M-digestion buffer (2×, Zymo D5021-9), 1 ml Proteinase K (20 mg ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from three biological replicates of E/I networks composed of Ngn2 #2 and Ascl1 #1 (DIV 49) with the Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from SARS-CoV-2 infected and uninfected Huh7 cells using the Direct-zol™ RNA Miniprep (Zymo Research, USA).
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bioRxiv - Microbiology 2021Quote: RNA from homogenized virus-inactivated lung and brain tissues of SARS-CoV-2 infected animals was extracted using the Direct-zol RNA MiniPrep Kit (Zymo Research R2051) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA from SARS-CoV-2 infected and uninfected Huh7 cells and supernatent was extracted using the Direct-zol RNA Miniprep (Zymo Research, US) and quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... cultured neurons at 15 DIV were left untreated or treated for 48 h with 2 μM TTX and then processed for total RNA extraction using the kit Direct-zol™ RNA MicroPrep (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... C-SCCE0625), and ScriptCap 2′-O-Methyltransferase (Cellscript, C- SCMT0625) and purified by a Direct-zol RNA Microprep Kit (Zymo Research, D2060). The radiolabeled TOP RNAs (4 pmol ...
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bioRxiv - Immunology 2021Quote: ... followed by a final clean-up step by agarose-gel extraction (2% (w/v) gel) with Zymoclean Gel DNA Recovery kit (Zymo Research D4001). Yeast scFv display libraries were generated using the amplified VH and VL DNA libraries from above and the pYD1 yeast surface display vector (Addgene plasmid #73447 ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified cell lysates (2×108 cells) were used to purify total RNA using the Direct-zol RNA MiniPrep kit (Zymo Research, USA). One microgram of total RNA after Turbo DNase I (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from HL-7702 cell lines and 2 μg DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (ZYMO Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... cucullus vegetative cells (300,000 cells) and 2-week-old mature cysts (3,000,000 cells) using the Direct-zol RNA purification system (Zymo Research Crop., California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μl of 0.5 M EDTA was added to this mixture and RNA fragments were column purified (Zymo research Cat no-D4061) and eluted in RNAse free water ...
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bioRxiv - Bioengineering 2021Quote: ... Selection for pyrF mutations was performed with 5-fluoroorotic acid (5-FOA, Zymo Research, part number F9001-5) at a final concentration of 0.5 mg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA and RNA were extracted from fresh frozen tumor (n=2) and normal (n=3) samples using the ZR-Duet DNA/RNA MiniPrep extraction kit (ZYMO RESEARCH, Irvine, CA). Quantification was performed with a NanoDrop One™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... we prepared a series of 8 serial dilutions of a known mock microbial community consisting of 8 gram positive and negative bacteria and 2 yeast (Zymo Research, catalog # D6300), as previously described (31) ...
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bioRxiv - Microbiology 2022Quote: ... 2 extracted with tissue samples and 1 run with water samples) and 2 samples of DNA from mock bacterial communities (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Irvine CA) were prepared and sequenced ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was extracted from 2-10 mL of stationary cultures with the Zymo Research Midiprep gDNA kit (Zymo Research EUROPE GmbH, Germany). Library preparation and sequencing were performed at Novogene (UK ...
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bioRxiv - Genomics 2023Quote: ... Purification was performed according to manufacturer’s instructions and the converted DNA was then eluted in 32.5 µl nuclease-free water (17 µl × 2 times) through a column from the DNA Clean & Concentrator kit (Zymo Research, cat. no. D4003).
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bioRxiv - Zoology 2023Quote: ... for 3 min at 28 Hz in 2 mL microcentrifuge tubes in 1X sterile PBS or four volumes of DNA/RNA Shield (Zymo Research, Irvine, CA) and two 5 mm stainless steel grinding balls ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified samples were run on a 2% agarose gel and fragments of the correct size were purified using ADB Agarose Dissolving Buffer (Zymo D4001-1-100) and UPrep Micro Spin Columns (Genesee Scientific 88– 343) ...
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bioRxiv - Plant Biology 2024Quote: Each composite sample was placed in a 2 ml ZR BashingBead Lysis tube containing beads and ZR stabilization reagent (Zymo Research, Irvine, CA), and was mixed immediately in the field using a bead-beating TerraLyzer lysis system (Zymo Research ...
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bioRxiv - Bioengineering 2024Quote: ... we first made a PCR standard by gel extracting the 93-bp PCR amplicon produced from the GAPDH primer/probe set using a 2% agarose gel and Zymo DNA Gel Extraction kit (Zymo cat. no. D4007). The concentration of gel-extracted GAPDH amplicon was quantified with a QuBit high sensitivity dsDNA assay and then diluted in nuclease-free water to create 10-fold serial dilutions ranging from 1e9 to 1e2 molecules/uL ...
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bioRxiv - Genomics 2024Quote: ... and 2 µL of DNA isolated using the Quick-DNA Fecal-Soil Microbe kit or the Quick-DNA Miniprep kit (Zymo Research, Irvine, CA). Reactions were run in a BioRad C1000 Touch (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
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bioRxiv - Plant Biology 2023Quote: ... Relative quantification of 5-methylcytosine (5-mC) over time was determined using the 5-mC DNA ELISA Kit (Zymo Research), using 100 ng of genomic DNA per sample as input ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and 3 swabs (2 for metagenomic DNA isolation, 1 for storage) moistened with DNA/RNA shield (Zymo Research, Cat. No. ZYR.R1100-250). Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e ...
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bioRxiv - Microbiology 2021Quote: ... were drawn from the intravenous line at 1- or 2-hour intervals and collected in DNA/RNA shield (Cat# R1150, Zymo Research, Irvine, CA, USA). All procedures were approved by SNUBH IACUC (BA1804-246/040-01).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Punches from each of these brain regions were placed in individual tubes containing 2□mm size beads (ZR BashingBeads Lysis Tubes, Zymo Research, item S6003-50) and 1 mL of QIAzol lysis reagent (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... and lung and nasal turbinate tissues were collected in 2-mL tubes containing ceramic beads filled with Tri Reagent (Zymo Research, #R2050-1-200). Tissues were homogenized using an Omni Bead Ruptor 24 and then centrifuged to remove debris ...
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bioRxiv - Physiology 2024Quote: ... Complementary DNA (cDNA) was synthesized from 1 to 2 μg of RNA using High-Capacity RNA-to-cDNA™ Kit (Zymo Research, Irvine, CA). Quantitative real-time polymerase chain reaction (qPCR ...
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bioRxiv - Microbiology 2020Quote: ... RNase R (Lecigen) treatment and follow-up purification (RNA Clean and Concentrator, Zymo) was performed as described in (36) ...
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bioRxiv - Genomics 2024Quote: ... 5 µL DNA Digestion Buffer and 5 µL DNase I (Zymo Research; R1013) was added ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: RNA was isolated from the cell culture supernatant of SARS-CoV-2-infected cells using the Quick-RNA Viral Kit (Zymo, California, USA cat no. R1035) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: The expression vectors were introduced into the yeast PichiaPink™ Strain 2 using Frozen-EZ Yeast Transformation II™ (Zymo Research Co., Irvine, CA, USA). Recombinant strains were initially cultured in BMGY liquid medium at 30 °C for about 24 h ...
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bioRxiv - Microbiology 2023Quote: ... containing a known concentration and taxonomy of 8 bacterial and 2 fungal species was also included as an internal control (Zymo Research Corp, Cat No. D6310). Extracted nucleic acids from each sample were randomized into a 96-well rack (Figure 1C ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for the estimation of global 5-hmC and 5-mC levels by ELISA using the Quest 5-hmC ELISA kit and the 5-mC DNA ELISA kit (Zymo research Inc, USA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... or a combination of TRIzol and the RNA Clean & Concentrator-5 (RCC-5) kit (Zymo Research), according to the Zymo instructions for combination Trizol/Zymo-Spin-Column RNA isolation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.075% 5-fluorooritic acid (Zymo Research)) ...
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bioRxiv - Genomics 2020Quote: ... We verified the presence of high molecular weight DNA products in the samples before purifying nucleic acids by Zymo DNA Clean & Concentrator-5 (ZYMO Research).
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bioRxiv - Synthetic Biology 2020Quote: ... and DCC-5 columns (Zymo Research). The sublibraries were pooled and sequenced on a single lane of a HiSeq4000 (Illumina) ...
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bioRxiv - Genomics 2020Quote: ... and then the fragmented DNAs were purified with DNA Clean & Concentrator-5 Kit (Zymo Research, DCC-5) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and 3×104-5×104 cells were collected for RNA isolation (RNA Clean & Concentrator-5, Zymo # R1013). The isolated RNA was hybridized with a nCounter custom probe set (108 probes ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cell suspension was mixed with 5 µl of 5 U/μl Zymolyase (Zymo Research, catalog no. E1005) and incubated for 1 h at 37°C with agitation ...