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Citations for Zymo Research :
151 - 200 of 1369 citations for E 6 2 6 6 Trimethylcyclohex 2 en 1 yl hex 5 ene 2 4 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from SARS-CoV-2 infected and uninfected Huh7 cells using the Direct-zol™ RNA Miniprep (Zymo Research, USA).
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bioRxiv - Microbiology 2021Quote: RNA from homogenized virus-inactivated lung and brain tissues of SARS-CoV-2 infected animals was extracted using the Direct-zol RNA MiniPrep Kit (Zymo Research R2051) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA from SARS-CoV-2 infected and uninfected Huh7 cells and supernatent was extracted using the Direct-zol RNA Miniprep (Zymo Research, US) and quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... cultured neurons at 15 DIV were left untreated or treated for 48 h with 2 μM TTX and then processed for total RNA extraction using the kit Direct-zol™ RNA MicroPrep (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... C-SCCE0625), and ScriptCap 2′-O-Methyltransferase (Cellscript, C- SCMT0625) and purified by a Direct-zol RNA Microprep Kit (Zymo Research, D2060). The radiolabeled TOP RNAs (4 pmol ...
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bioRxiv - Immunology 2021Quote: ... followed by a final clean-up step by agarose-gel extraction (2% (w/v) gel) with Zymoclean Gel DNA Recovery kit (Zymo Research D4001). Yeast scFv display libraries were generated using the amplified VH and VL DNA libraries from above and the pYD1 yeast surface display vector (Addgene plasmid #73447 ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified cell lysates (2×108 cells) were used to purify total RNA using the Direct-zol RNA MiniPrep kit (Zymo Research, USA). One microgram of total RNA after Turbo DNase I (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from HL-7702 cell lines and 2 μg DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (ZYMO Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... cucullus vegetative cells (300,000 cells) and 2-week-old mature cysts (3,000,000 cells) using the Direct-zol RNA purification system (Zymo Research Crop., California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μl of 0.5 M EDTA was added to this mixture and RNA fragments were column purified (Zymo research Cat no-D4061) and eluted in RNAse free water ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA and RNA were extracted from fresh frozen tumor (n=2) and normal (n=3) samples using the ZR-Duet DNA/RNA MiniPrep extraction kit (ZYMO RESEARCH, Irvine, CA). Quantification was performed with a NanoDrop One™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... we prepared a series of 8 serial dilutions of a known mock microbial community consisting of 8 gram positive and negative bacteria and 2 yeast (Zymo Research, catalog # D6300), as previously described (31) ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was extracted from 2-10 mL of stationary cultures with the Zymo Research Midiprep gDNA kit (Zymo Research EUROPE GmbH, Germany). Library preparation and sequencing were performed at Novogene (UK ...
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bioRxiv - Genomics 2023Quote: ... Purification was performed according to manufacturer’s instructions and the converted DNA was then eluted in 32.5 µl nuclease-free water (17 µl × 2 times) through a column from the DNA Clean & Concentrator kit (Zymo Research, cat. no. D4003).
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bioRxiv - Zoology 2023Quote: ... for 3 min at 28 Hz in 2 mL microcentrifuge tubes in 1X sterile PBS or four volumes of DNA/RNA Shield (Zymo Research, Irvine, CA) and two 5 mm stainless steel grinding balls ...
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bioRxiv - Plant Biology 2024Quote: Each composite sample was placed in a 2 ml ZR BashingBead Lysis tube containing beads and ZR stabilization reagent (Zymo Research, Irvine, CA), and was mixed immediately in the field using a bead-beating TerraLyzer lysis system (Zymo Research ...
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bioRxiv - Bioengineering 2024Quote: ... we first made a PCR standard by gel extracting the 93-bp PCR amplicon produced from the GAPDH primer/probe set using a 2% agarose gel and Zymo DNA Gel Extraction kit (Zymo cat. no. D4007). The concentration of gel-extracted GAPDH amplicon was quantified with a QuBit high sensitivity dsDNA assay and then diluted in nuclease-free water to create 10-fold serial dilutions ranging from 1e9 to 1e2 molecules/uL ...
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bioRxiv - Genomics 2024Quote: ... and 2 µL of DNA isolated using the Quick-DNA Fecal-Soil Microbe kit or the Quick-DNA Miniprep kit (Zymo Research, Irvine, CA). Reactions were run in a BioRad C1000 Touch (Bio-Rad Laboratories ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Punches from each of these brain regions were placed in individual tubes containing 2□mm size beads (ZR BashingBeads Lysis Tubes, Zymo Research, item S6003-50) and 1 mL of QIAzol lysis reagent (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: RNA was isolated from the cell culture supernatant of SARS-CoV-2-infected cells using the Quick-RNA Viral Kit (Zymo, California, USA cat no. R1035) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: The expression vectors were introduced into the yeast PichiaPink™ Strain 2 using Frozen-EZ Yeast Transformation II™ (Zymo Research Co., Irvine, CA, USA). Recombinant strains were initially cultured in BMGY liquid medium at 30 °C for about 24 h ...
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bioRxiv - Microbiology 2023Quote: ... containing a known concentration and taxonomy of 8 bacterial and 2 fungal species was also included as an internal control (Zymo Research Corp, Cat No. D6310). Extracted nucleic acids from each sample were randomized into a 96-well rack (Figure 1C ...
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bioRxiv - Genetics 2023Quote: ... Microinjections were performed with 4-5 µg/µl (diluted in nuclease free water, Zymo Research) fas5 and GFP dsRNA on a Femtojet microinjector (Eppendorf ...
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bioRxiv - Molecular Biology 2022Quote: ... we used Zymo-Spin V-E (#C1024; Zymo Research) columns which are included in Zymo Research RNA extraction midi kits or can be purchased separately ...
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bioRxiv - Genomics 2022Quote: ... followed by max speed centrifugation at 4 °C for 5 minutes and DNA purification by column purification (Zymo) prior to sample preparation for next-generation sequencing.
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bioRxiv - Genomics 2023Quote: ... and gDNA wash buffers from Zymo (#D3004-1, #D3004-5). DNA library preparation was also performed in 96-well plates ...
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bioRxiv - Molecular Biology 2024Quote: ... Strep-Tactin resin (Zymo Research, catalog number P2004-1-5), or S-protein agarose (Novagen ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli DH5α (Zymo Mix & Go E. coli Transformation Kit, Zymo Research #T3001). Each construct was verified by Sanger sequencing (Australian Genome Research Facility ...
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bioRxiv - Cancer Biology 2020Quote: Approximately 1.4kb of genomic fragment spanning SALL4 5’ UTR - exon 1 - intron 1 was PCR amplified (Zymo Research) and cloned into the pGEM-T Easy vector ...
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Synthetic Biology 2024Quote: ... and transformed into MegaX cells made chemically competent (Mix & Go E. Coli Transformation Kit, Zymo Research). The top 8 variants of each library selection were constructed ...
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bioRxiv - Microbiology 2024Quote: Bacterial genomic DNA was extracted by using e Quick-DNA™ Miniprep Plus Kit (Zymo Research) according to the manufacturer’s instruction as the template ...
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bioRxiv - Developmental Biology 2021Quote: ... and submerged in digest solution containing 1X Digest Buffer (Zymo Research, Cat#: D3050-1-5) and supplemented with 1mg/mL Proteinase K (Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... and from pool BFU-E and single colonies using the Quick-DNA/RNA Miniprep kit (ZYMO Research). RNA was treated with DNase using the DNase I kit (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... Selection for pyrF mutations was performed with 5-fluoroorotic acid (5-FOA, Zymo Research, part number F9001-5) at a final concentration of 0.5 mg/ml ...
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bioRxiv - Genomics 2024Quote: ... and 5 µL of DNA Digestion Buffer and 1 µL of DNase I (Zymo Research; R1013) were added ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 % ethanol was added to the aqueous phase (1:1, v/v) and transferred to RNA Clean and Concentrator (RCC) ™ −5 kit (Zymo Research; R1013) and centrifuged at 16,000 x g for 30 s ...
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bioRxiv - Genetics 2020Quote: ... Reactions were then quenched by the addition of 1 volume DTT 1 M and then purified on an RNA Clean & Concentrator-5 column (Zymo Research, cat. R1013).
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bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
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bioRxiv - Plant Biology 2023Quote: ... Relative quantification of 5-methylcytosine (5-mC) over time was determined using the 5-mC DNA ELISA Kit (Zymo Research), using 100 ng of genomic DNA per sample as input ...
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bioRxiv - Synthetic Biology 2021Quote: ... Complementary plasmids were co-transformed into competent MC1061ΔpspF cells carrying the reporter plasmid pLY54 (prepared using the Mix & Go E. coli Transformation Kit from Zymo Research ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α λπ (prepared in-lab with Mix & Go! E. coli Transformation Kit and Buffer Set from ZYMO Research). Plasmid DNA was isolated using E.Z.N.A ...
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bioRxiv - Genomics 2024Quote: ... 5 µL DNA Digestion Buffer and 5 µL DNase I (Zymo Research; R1013) was added ...
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bioRxiv - Microbiology 2023Quote: ... the whole gastrointestinal tract of zebrafish was then carefully dissected out and stored in an MP Biomedicals Lysing E Matrix tube with 0.5 ml of 1X DNA/RNA Shield (Zymo research). A total of three whole gastrointestinal tracts were collected.
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bioRxiv - Microbiology 2023Quote: ... fecal matter was then transferred to and stored in an MP Biomedicals Lysing E Matrix tube with 0.5 ml of 1X DNA/RNA Shield (Zymo research). A total of three replicate fecal samples were collected.
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bioRxiv - Microbiology 2023Quote: ... approximately ½ of a 10 ul loop of the intestinal content was then transferred to an MP Biomedicals Lysing E Matrix tube with 0.5 ml of 1X DNA/RNA Shield (Zymo research). Four intestinal-content samples were collected.
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bioRxiv - Microbiology 2023Quote: ... approximately ½ of a 10 ul loop fecal matter was then transferred to and stored in an MP Biomedicals Lysing E Matrix tube with 0.5 ml of 1X DNA/RNA Shield (Zymo research). A total of three “squeezed gut” samples were collected.
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bioRxiv - Cancer Biology 2022Quote: ... was used for the estimation of global 5-hmC and 5-mC levels by ELISA using the Quest 5-hmC ELISA kit and the 5-mC DNA ELISA kit (Zymo research Inc, USA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...