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Citations for Zymo Research :
151 - 200 of 516 citations for 6 TRIFLUOROMETHYL 1 2 3 4 TETRAHYDRO ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and genomic DNA was extracted from 3 mL of overnight (TSB) broth culture using the ZR Duet DNA/RNA MiniPrep Plus kit (Zymo Research) following manufacturer’s protocol for the “suspended cells” option ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Microbiology 2022Quote: ... The high molecular weight bands were excised with a razor and dissolved in 3 volumes of Zymo Agarose Dissolving Buffer (Zymo D4001) at 50°C ...
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bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Genetics 2022Quote: ... cells were synchronized in G1 phase by addition of 4 µg/mL α-factor (Zymo research, mating hormone peptide) for 2 h ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from 2 × 106 cells using the Quick-RNA miniprep kit (Zymo) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: RNA was extracted from 2 dpf whole embryos using the Directzol RNA mini kit (Zymo). cDNA was synthesised and amplified using Thermo Fisher SuperScript II Reverse Transcriptase Kit ...
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bioRxiv - Genetics 2019Quote: Genomic DNA (2 μg) was modified by bisulfite treatment (EZ DNA methylation kit, Zymo Research) and amplified by FastStart DNA polymerase (Roche) ...
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bioRxiv - Genetics 2019Quote: Genomic DNA (2 μg) was modified by bisulfite treatment (EZ DNA methylation kit, Zymo Research) and amplified by FastStart DNA polymerase (Roche) ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (300µl) was mixed with 30µl of Proteinase K (Zymo Cat #D3001-2-20) digestion buffer and 15µl of Proteinase K in a new tube using a brief vortex step ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2-infected and Mock-infected samples were treated with RNA Shield (Zymo Research) to preserve their genetic integrity and inactivate the virus ...
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bioRxiv - Genomics 2022Quote: ... then thawed and combined with 2 volumes of DNA Shield (Zymo Research, Irvine, CA, USA). Genomic DNA was extracted using a Quick DNA Miniprep Plus kit (Zymo Research ...
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bioRxiv - Genomics 2022Quote: ... into the 20 μl ligation reaction for incubation with 30 minutes at 30°C and 3 minutes at 65°C to inactivate the enzymes followed by column purification (Zymo Research, R1016). Before the reverse transcription (RT) ...
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bioRxiv - Plant Biology 2020Quote: Genomic DNA was extracted from aerial parts of 3-week-old seedlings using Quick-DNA Plant/Seed Miniprep Kit (Zymo research, D6020) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: A PCR of all constructs using forward primer pTD2F (5’-TTGGCACCAAAATCAACGGG–3’) and reverse primer SP6 was carried out with SuperFi DNA Polymerase (ThermoFischer Scientific, USA) and agarose gel purified (Zymo Research, USA). These primers amplify the gene insert ...
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bioRxiv - Zoology 2024Quote: ... Clean-up used a combination of two kits: the beads from the Select-a-size Magbead kit (Zymo Cat# D4084-3-10) and the buffers from Genomic DNA Clean & Concentrator -25 (Zymo Cat# D4064) ...
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bioRxiv - Physiology 2024Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick-RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Physiology 2023Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick- RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
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bioRxiv - Molecular Biology 2019Quote: ... Following phenol/chloroform and EtOH precipitation the pellets were dissolved in 300ul 10mM Tris pH7.5 and DNA was further purified using 4 Zymo columns per sample (Zymo, D4014). Each sample was eluted in 200ul total of 10mM Tris pH7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Samples were then transferred in 4 ml tubes and processed for total RNA extraction with RNA micro kit (ZYMO REAGENT) according to company protocol ...
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bioRxiv - Immunology 2021Quote: ... Samples were stored in RNAlater (Invtrogen) for 24 hours at 4°C and then homogenized in TRI Reagent (Zymo Research) with a beadbeater ...
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bioRxiv - Microbiology 2023Quote: ... 4 ml of the cultures were used for total RNA extraction using the Direct-zol RNA extraction kit (Zymo Research) and TRI Reagent (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Tumors were then harvested 4 weeks post-injection and processed using Quick-DNA midiprep plus kit (Zymo Research Cat. #D4075). For each processed tumor ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from CaCo-2 cells using Direct-zol™ RNA Microprep (ZYMO cat: R2062), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Plasmids of sorted and unsorted populations where extracted using the Zymo prep kits (Zymo, version 2) with modifications as described previously19 ...
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bioRxiv - Physiology 2020Quote: ... from all the experimental groups (n=3/group) was subjected to bisulphite treatment using the EZ DNA methylation™ kit (Zymo Research, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... one set of replicates (total of 3 replicates per treatment) was harvested for DNA and RNA using ZR-Duet DNA/RNA MiniPrep (Plus) kit (Zymo Research Cat # D7003) following manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... 4-S-UTP containing RNA was eluted in 100mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAse reaction to eliminate traces of genomic DNA ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 1 ml of sediment slurry was serially diluted and 100 μL was plated in 10−4 dilution in triplicate on MB2216 agar plates using rattler beads (Zymo #S1001); colony forming units (CFU ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from Vero cells after 4 days post infection using the Direct-zol RNA isolation kit (Zymo Research). 1 μg of RNA isolate was subjected to rRNA removal with the NEBNext rRNA Depletion Kit (NEB ...
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bioRxiv - Bioengineering 2022Quote: The isolated yeast populations obtained after round 4 were subjected to colony screen and plasmid recovery by using Zymoprep kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Genomics 2020Quote: ... 4-SUTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAseq reaction to eliminate traces of genomic DNA ...
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bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged 1 min at 12000 g at 4°C and RNAs were extracted from supernatants with Direct-zol RNA Mini Prep kit (Zymo Research), following manufacturer’s protocol and including the optional DNAse treatment step ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from rosette tissue harvested at ZT 23 on day 25 post imbibition (n = 4 biological replicates) using the Direct-zol-96 MagBead RNA kit (Zymo Research). RNA was checked for degradation using a Tapestation 4150 (Agilent ...
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bioRxiv - Bioengineering 2023Quote: ... The lysates were centrifuged at 5000 x g for 10 min at 4 °C and supernatant added to gDNA extraction columns (Zymo Research). The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research ...
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Phage-centric ecological interactions in aquatic ecosystems revealed through ultra-deep metagenomicsbioRxiv - Microbiology 2019Quote: ... were cut in small pieces (≅ 3–5mm) using sterile scissors and processed for DNA extraction using the ZR Soil Microbe DNA MiniPrep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... Then, samples were centrifuged (14000 g, 3 min) and the supernatants were transferred to silica columns to bind DNA (Zymo Research, Irvin, California, USA). After 1 min of incubation ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mL of suspended biomass was collected in triplicate from the mixed tank and PBR effluent and stored in 5 mL polypropylene transport tubes (FisherbrandTM, USA) prefilled with 3 mL DNA/RNA ShieldTM (Zymo Research corp., CA, USA). The samples were briefly vortexed and stored at -20 °C until they were shipped to University at Buffalo (UB ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Genomics 2022Quote: ... for 2 hours at 37C then cleaned using a Zymo DNA Clean & Concentrator column-5 (Zymo #D4013). The cleaned RCA product was digested using NEBNext dsDNA Fragmentase (NEB #M0348 ...
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bioRxiv - Biochemistry 2020Quote: ... The released RNA was then cleaned up by adding 2 volumes of RNA Lysis Buffer (Zymo Research) and one volume of 100% ethanol ...
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bioRxiv - Microbiology 2022Quote: pAd/SARS-CoV-2-S1Beta was amplified and purified using ZymoPURE II plasmid maxiprep kit (Zymo Research). For Expi293 cell transfection ...
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bioRxiv - Microbiology 2023Quote: ... and 2) amplification of DNA extracted from a ZymoBIOMICS Microbial Community Standard (Zymo Research, Irvine, CA U.S.A.). Contaminating ASVs were identified through the use of the decontam package 84 ...
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bioRxiv - Genomics 2023Quote: ... 2 µl adapter filling mixture containing 0.375 mM 5-Methylcytosine dNTP Mix (Zymo Research, cat. no. D1030), 0.75 µl of Thermopol Reaction buffer (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... filled with 1% FBS-HBSS or Tri Reagent (Zymo, #R2050-1-200). Tissue was homogenized in an Omni Bead Ruptor 24 (5.15 ms ...
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bioRxiv - Genomics 2020Quote: DNA was extracted using 4-10 unstained slides of FFPE tissue using the Pinpoint Slide DNA Isolation system (Zymo Research, Orange, CA). In brief ...
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bioRxiv - Plant Biology 2019Quote: Total RNA from 4 weeks old Arabidopsis thaliana WT Col-0 and mta mutant plants was isolated using Direct-zol™ RNA kit (Zymo Research). RNA was quantified by Qubit RNA Assay Kit (Life Technologies ...