Labshake search
Citations for Zymo Research :
1601 - 1650 of 1848 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: One leaf tissue of 4- to 5-week-old plants with similar age from fwa and early flowering ZF transgenic lines were collected for RNA extraction using Direct-zol RNA MiniPrep kit (Zymo Research). 1μg of total RNA was used to prepare the libraries for RNA-seq following TruSeq Stranded mRNA kit (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from primary neurons was extracted using home-made TRIzol reagent in combination with Direct-zol RNA Kit (Zymo Research). The samples were fixed in cold methanol for 10 mins at −20C ...
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bioRxiv - Genetics 2022Quote: ... 500 ng of genomic DNA from each sample was bisulfite-converted with the EZ DNA Methylation™ Kit (Zymo Research, D5002) and analyzed using Infinium HumanMethylationEPICBeadChip™ Kit (Illumina ...
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bioRxiv - Immunology 2022Quote: RNA was isolated from TRI Reagent samples using phenol-chloroform extraction or column-based extraction systems (Direct-zol RNA Miniprep, Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: RNAlater-preserved samples were homogenized in PBS and total RNA was extracted from samples using the Quick-RNA Miniprep Kit (Zymo Research). RNA extracts were evaluated for concentration and purity using a Nanodrop 8000 instrument (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from neocortical regions of slice cultures using Trizol reagent followed by RNA Clean & Concentrator kit (Zymo research R1014). cDNA was synthesized with 0.5 μg of RNA using the cDNA Synthesis Kit (Bio-Rad 170-8891 ...
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bioRxiv - Systems Biology 2023Quote: ... to exclude small fragments (less than about 200bp) and the DNA was eluted from the gel slices using the Zymoclean Gel DNA Recovery Kit (Zymo, #D4001), followed by quantification (Qubit ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 2-5 x107 cells grown in different nutrients (see above) with the YeaStar RNA Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... the dsDNA target was PCR amplified from the plasmid containing the protospacer and purified by DNA Clean & Concentrator-25 (Zymo Research). The reaction was initiated with incubating 1 μM Casπ RNP effectors with 20 nM dsDNA target at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X ...
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bioRxiv - Genomics 2022Quote: ... 400ng of genomic DNA from 406 participants was subjected to bisulfite conversion using the EZ-96 DNA Methylation-Goldkit (Zymo Research). Two PCR products were amplified ...
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bioRxiv - Immunology 2023Quote: ... DNA obtained from anti-OVA hits were then cut out from agarose gels and amplicons from heavy and light chains were purified separately by gel extraction (Zymoclean Gel DNA Recovery Kit, Zymo Research).
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bioRxiv - Genomics 2023Quote: ... Tissue was then homogenized with steel beads and total RNA was extracted using the Direct-zol™ RNA MicroPrep kit from Zymo Research ...
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bioRxiv - Evolutionary Biology 2022Quote: RNA was extracted from 30 μL of each virus inoculum using a Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: A standard whole-cell mixture consisting of 21 representative strains from 17 species of the gut microbiota (Zymo Research, ref D6331) was divided into 10 aliquots and stored in the storage solution provided by the manufacturer at −80 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: DNase-treated RNA prepped from one well of a six well plate (approximately 106 cells) was extracted using Quick-RNA MiniPrep (Zymo Research) from human U251-MG glioblastoma cells (ECACC 09063001 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA was isolated from rat kidney samples and PTC cultures using Trizol + Direct-zol columns according to the referenced protocol (Zymo Research). For PTC cultures ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA was isolated from Transwell filters (multiple filters were pooled per timepoint) and concentrated over RNA Clean & Concentrate (RCC) columns (Zymo Research). RNA quality was assessed by Agilent Bioanalyzer and all samples had a RIN > 6.3 ...
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bioRxiv - Microbiology 2024Quote: ... 250 µl aqueous phase was mixed 1:1 with 75% ethanol and transferred into the Zymo-Spin IC Column from the RNA Clean & Concentrator Kit (Zymo Research). RNA clean-up and collection was done following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: RNA was extracted from callus and protoplast cultures using the ZR Plant RNA Miniprep™ kit (R2024, Zymo Research, CA, USA) and TRIzol™ Reagent (15596026 ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 1.5 mL cultures of Bacteroides strains using the Zymobionics DNA Miniprep kit (Zymo Research, Irvine, CA) and used as input for IS-Seq as described previously 19 ...
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bioRxiv - Cell Biology 2024Quote: ... mitochondria and mitoplasts isolated from magnetic-bead IP (40 ng) methods were converted to cDNA libraries (Zymo-Seq RiboFree Total RNA #R300, Zymo Research) then underwent whole transcriptome RNA sequencing as described above.
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bioRxiv - Developmental Biology 2024Quote: Total RNA was isolated from 1-day old zebrafish embryos with TRI Reagent using Direct-zolTM RNA MicroPrep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... RNA from three biological replicates of infected leaves and fungal mycelium were extracted using Quick-RNA Fungal/Bacteria Miniprep (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2024Quote: ... Adapter-ligated fragments of 150-250bp and 250-300bp were recovered from a 2/5% NuSieve 1:1 agarose gel (Zymoclean Gel DNA Recovery Kit, Zymo Research). The fragments were then bisulfite treated using EX DNA Methylation-Lightning Kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were removed from its plastic housing using a razor blade and placed in 550 µL of RNA lysis buffer (Zymo R1050) at room temperature for 15 minutes to lyse the neurites ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was isolated from the fresh feces collected from UT1- or PBS-gavaged mice (female, n = 4/group) using a ZymoBIOMICS DNA Miniprep Kit (Zymo Research). Note ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from these samples as previously described (19) using a ZymoBiomics DNA Miniprep kit (Zymo Research, Irvine, CA USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from cells expressing the iRS3 reporter 3h post-induction using the Direct-zol™ RNA MiniPrep kit (Zymo). After extraction ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from infected cells 72 h post infection using Quick-DNA Miniprep kit (Zymo Research, catalogue no. D3024).
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bioRxiv - Genomics 2024Quote: ... An aliquot of 250 ng DNA from each sample was bisulfite converted using the EZ DNA Methylation kit (Zymo Research, #D5001) according to manufacturer’s instructions for Illumina DNA methylation arrays ...
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bioRxiv - Biochemistry 2024Quote: DNase-treated RNA prepped from one well of a six well plate (approximately 105 cells) was extracted using Quick-RNA MiniPrep (Zymo Research) from indicated cells ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of every amplicon from the same RT product was pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: DNA was isolated from freshly-grown colonies using the Zymo Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA). Libraries were prepared using the Nextera DNA Flex Library Prep as recommended by the manufacturer (Illumina ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from 1*106 WT or sorted SLAMF6-KO NK-92 cells using an RNA Micro Kit (Zymo Research). After PolyA selection libraries were prepared and lllumina sequencing of 20-30 million paired-end reads performed ...
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bioRxiv - Systems Biology 2024Quote: ... We isolated the transposed and adaptor-ligated genomic DNA from the reaction with the Zymo DNA Clean and Concentrator--5 Kit (Zymo #D4014) and then amplified the libraries using the custom primers described in Supplemental Table 4 (originally designed by the William Greenleaf lab at Stanford University) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Genomic DNA was extracted from the hippocampus using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research, Irvine, CA, United States) and checked for purity and quantity using a NanoDrop spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... followed by resuspension of ∼200k to 1 million cells in 300ul RNA Lysis Buffer from the Quick-RNA Miniprep Kit (Zymo R1055) and stored in −70deg ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was prepared from 1×106 cells of HAP1 and HELLS KO using Direct-zol RNA Microprep kit (Zymo Research). The RNA was checked for quality using the Agilent BioAnalzyer 2100 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from TRAP and input samples with TriZol and the Direct-Zol RNA MicroPrep Kit (Zymo Research, Orange, CA).
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bioRxiv - Genomics 2024Quote: Genomic DNA from placental tissues was isolated using a Quick-DNA mini prep plus kit according to the manufacturer’s instructions (Zymo Research D4068). LINE-1 methylation levels were quantified using an ELISA-based Global DNA Methylation Assay LINE-1 kit ...
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bioRxiv - Neuroscience 2024Quote: RNA extraction and quantitative reverse transcription polymerase chain reaction (qRT-PCR) Total RNA was extracted from fresh frozen mouse cerebral cortex tissues using Direst-zol RNA miniprep (R2052, Zymo Research). The extracted RNA was reverse-transcribed into cDNA using the High-Capacity cDNA Reverse Transcription Kit (4368814 ...
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bioRxiv - Cancer Biology 2024Quote: ... was incubated for 30 minutes on a rotating orbital.DNA recovered from dynabeads was purified with a DNA clean and Concentrator-5 kit (Zymo Research, D4013). Amplification of the recovered DNA was carried out using a whole genome amplification kit (WGA2 ...
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bioRxiv - Bioengineering 2024Quote: ... Genomic DNA from one full-scale lentiviral infection replicate was extracted using Quick-DNA Midiprep Plus kit and protocol (Zymo, D4075). An Alt-R S.p ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from iPSC control and iPSC-MPPAX3/7-FOXO1 cells (in three replicates) using RNA Zymo-Spin columns (Zymo R1015). Before library preparation ...
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bioRxiv - Cell Biology 2024Quote: Genomic DNA was extracted from HG-cultured β-cell lines and processed using the EZ-DNA Methylation Direct Kit (Zymo Research). The treated DNA was amplified by PCR and purified with a gel extraction kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA extraction from drug-resistant cells was performed using the Zymo QuickDNA™ Midiprep Plus Kit (Zymo Research, Cat. D4068), according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was extracted from 12-day old gametophytes using Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, Irvine, CA, USA). Primers were designed using SnapGene with elongation factor 1 (Ceric.09G081400 ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from 100 mg of ground tissue following the protocol of the Quick-RNA Miniprep kit (Zymo Research). The integrity of the RNA samples was assessed using the RNA Integrity Number (RIN) ...
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bioRxiv - Developmental Biology 2024Quote: We isolated genomic DNA from all samples using the Zymo Quick DNA mini-prep Plus Kit (Zymo Research; Cat. no. D4068). The samples were amplified and prepared for sequencing as described previously (Gilbert et al ...
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bioRxiv - Genetics 2021Quote: DNA bisulfite conversion was performed directly starting from cell pellets (a maximum of 1×105 cells per sample) using the EZ DNA Methylation-Direct kit (Zymo Research #D5021) following the manufacturer’s instructions ...