Labshake search
Citations for Zymo Research :
1451 - 1500 of 6880 citations for Estrone 3 Sulfate E1S ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: ... RNA was extracted using quick-RNA MiniPrep Kit (Zymo) and used to generate complementary DNA using qScript cDNA synthesis kit (Quantabio) ...
-
bioRxiv - Microbiology 2024Quote: ... and processed with an RNA Clean & Concentrate kit (Zymo). The next-generation sequencing (NGS ...
-
bioRxiv - Microbiology 2024Quote: ... and processed with an RNA Clean & Concentrate kit (Zymo) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... purified via Zymoclean Gel DNA Recovery Kit (Zymo Research), and submitted for Sanger sequencing to Quintara Biosciences.
-
bioRxiv - Neuroscience 2024Quote: ... per manufacturer recommendations followed by Zymo Direct-zol column purification kit (Zymo Research, Cat # R2062). Library preparation was performed in 2 equal batches by Salk’s Next Generation Sequencing Core ...
-
bioRxiv - Molecular Biology 2024Quote: ... and a Direct-zol RNA MicroPrep Kit (Zymo Research). After rRNA depletion (see the “Library preparation for ribosome profiling” section) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and a Direct-zol RNA MicroPrep Kit (Zymo Research). After separation on a 15% urea polyacrylamide gel electrophoresis (PAGE ...
-
bioRxiv - Microbiology 2024Quote: ... and the RNA Clean and Concentrator kit (Zymo Research). The RNA was DNase-treated using the TURBO DNA-freeTM kit (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... or the Quick-DNA/RNA Microprep Plus Kit (Zymo), and RNA quantity and purity were determined by a NanoDropOne spectrophotometer (Nanodrop Technologies) ...
-
bioRxiv - Genomics 2020Quote: Genomic DNA was isolated with the NucleoSpin Tissue kit (Macherey&Nagel, Düren, Germany) and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Pyrosequencing was performed on a PyroMark ID System (Biotage ...
-
bioRxiv - Microbiology 2020Quote: ... The total DNA from each bacterium was extracted using a DNA extraction kit (ZR soil microbe DNA MiniPrep™ kit (Zymo Research, USA) according to the manufacturer’s instructions [35] ...
-
bioRxiv - Synthetic Biology 2020Quote: ... MCs were further cleaned to remove any PP contamination using Plasmid-Safe ATP Dependent DNase kit (Epicenter, WI, USA) followed by the DNA Clean & Concentrator Kit (Zymo Research, CA, USA).
-
bioRxiv - Physiology 2019Quote: DNA samples from cells and tissue were isolated using Qiagen’s DNA easy blood and tissue kit (Manchester, UK) and bisulfite converted using Zymo Research EZMethylation Kit (Zymo Research, Irvine, CA, USA) as per manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: ... Nucleic acid was isolated with either the Quick Microprep Plus kit (Catalog D7005) or the Zymo Quick Miniprep Plus kit (Catalog D7003) (Zymo Research, Irvine, CA). Gel electrophoresis was performed as described above ...
-
bioRxiv - Developmental Biology 2023Quote: ... Plasmid and fosmid DNA was purified with either the Zyppy Plasmid Miniprep kit or ZymoPURE II Plasmid Midiprep kit (Zymo Research, Irvine, CA). Following restriction digests or PCR ...
-
bioRxiv - Microbiology 2023Quote: ... control samples (n = 4, one for each extraction batch) using commercially available kits optimized for fecal-based microbial communities (Zymo fecal/soil kit), and some aliquots archived ...
-
bioRxiv - Cell Biology 2023Quote: ... and RNA was isolated using either a commercial kit (53BP1 and MDM2i RNAseq experiments; Direct-zol RNA Miniprep Kits, Zymo Research Cat#R2050) or with chloroform according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... purified PCR product was digested with EcoRI-HF and BamHI-HF at 37°C for 1.5 hours and purified again using a Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). For the cloning backbone ...
-
bioRxiv - Developmental Biology 2019Quote: ... Sorted cells were collected in PBS, centrifuged (2000 rpm, 5 min) and RNA extracted using the Quick RNA Mini Prep (Zymo Research) according to manufacturer’s protocol.
-
bioRxiv - Genomics 2019Quote: ... The poly(A)-tailed Mb3Cas12a mRNAs were purified using the RNA Clean & Concentrator™-5 (Cat. R1016, Zymo Research, Irvine, CA) and eluted in a Tris-EDTA solution (Cat.11-01-02-02 ...
-
bioRxiv - Genetics 2020Quote: ... all PCR products were cleaned using a Zymo Research ZR-96 DNA Clean & Concentrator™-5 (Zymo Research, Irvine, California, USA). Samples were eluted in 50µl of DNA free water.
-
bioRxiv - Bioengineering 2020Quote: ... at 37°C for 24 hours to ensure complete removal of any 5’-triphosphate moieties then purified again using a RNA spin column (Zymo Research). Lastly ...
-
bioRxiv - Biochemistry 2021Quote: ... The cleared supernatant was then transferred to a fresh reaction tube and applied to spin-columns (RNA Clean & Concentrator-5 columns, Zymo-Research) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: DNA samples ranging from 500 ng-2 μg were digested to free nucleosides using 5-15 U of DNA Degradase Plus (Zymo Research) in 25 μl reactions incubated for 2 hrs at 37 °C ...
-
bioRxiv - Genomics 2020Quote: ... The reaction was then treated with DNase I to remove template DNA and RNA product was purified using an RNA Clean and Concentrator-5 (Zymo #R1016). Purified RNA was used as the template for reverse transcription using ProtoScript II according to manufacturer protocol ...
-
bioRxiv - Bioengineering 2022Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the DNA template via in vitro transcription (IVT ...
-
bioRxiv - Microbiology 2020Quote: ... Most of the supernatant was removed and 750 μl of ZymoBIOMICS Lysis Solution and 19 μl of proteinase K (D3001-2-20/D3001-2-5, Zymo Research) were added to the remaining 200 μl of the samples and incubated for 30 min at +55 °C ...
-
bioRxiv - Microbiology 2019Quote: ... 1.5 µg of genomic DNA in 30 ml ddH2O was digested to free nucleosides using 5 U of DNA Degradase Plus (Zymo Research) in 25 μl reactions incubated for 2 hours at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: 15 µl of UltraPure water was added to each assembly reaction on ice and 2.5 µl of these diluted reactions were then transformed into 50 µl chemically competent DH5α cells (prepared in house, Zymo T3001) on ice ...
-
bioRxiv - Genomics 2022Quote: ... The samples were treated with DNase I and cleaned using ethanol precipitation and subsequent RNA Clean & Concentrator-5 spin-column purification (Zymo Research). Successful removal of genomic DNA was confirmed by PCR using a host-specific primer pair (Supplementary Figure S1) ...
-
bioRxiv - Microbiology 2024Quote: ... All 30 samples were pooled in an equivolume and purified with RNA Clean & Concentrator-5 columns (Zymo Research, Irvine, CA, USA). RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Invitrogen ...
-
bioRxiv - Genetics 2023Quote: ... Transposition reactions were incubated for 30 min at 37°C while shaking at 1,000 rpm and cleaned using DNA Clean & Concentrator-5 (Zymo Research, D4003). Transposed libraries were amplified in 50 μl PCRs (0.5 μl Q5 DNA polymerase ...
-
bioRxiv - Biophysics 2024Quote: ... or downstream of the poly(A) segment (Trichoplax AKDF19383) and purified via ethanol precipitation or DNA Clean & Concentrator-5 columns (Zymo Research). mRNA was transcribed for 2-2.5 h with mMESSAGE mMACHINE kits (ThermoFisher ...
-
bioRxiv - Microbiology 2022Quote: ... were incubated in a total volume of 20 µL for 30 min at 37°C and subsequently column-purified using RNA Clean and concentrator 5 (Zymo Research).
-
bioRxiv - Cell Biology 2023Quote: ... Viral gene sequences in pPolI plasmids for all PR8 segments were amplified by PCR using corresponding primer pairs and were purified using ZYMO Research DNA cleaner and Concentrator-5 (ZYMO, D4014). Purified PCR products were in vitro transcribed using the T7 RiboMAX Express Large Scale RNA Production System (Promega ...
-
bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at 37°C for 1 hour and then purified with Zymo DNA Clean & Concentrator-5 (Zymo Research) following the PCR product protocol and eluting DNA into 10.5 μL of water ...
-
bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and mixed with 450 μl ethanol and transferred to an RNA Clean & Concentrator-5 column (Zymo Research) for downstream RNA purification and concentration ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Products were checked on an agarose gel and subjected to a PCR-clean up step (DNA Clean and Concentrator-5, Zymo, D4013). A second PCR (PCR2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was purified by phenol-chloroform extraction and the aqueous fraction was further cleaned using DNA Clean & Concentrator-5 with Zymo-Spin Columns (Zymo Research). Libraries were built with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
-
bioRxiv - Bioengineering 2023Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the PCR DNA template via in vitro transcription (IVT ...
-
bioRxiv - Molecular Biology 2024Quote: ... The resulting RNA pellets were resuspended in water and digested with 2 U of DNAse I for 30 min at 37 °C followed by purification using RNA clean & concentrator 5 columns (Zymo Research). mRNA levels in every fraction was quantified by RT-qPCR as described above ...
-
bioRxiv - Molecular Biology 2024Quote: ... The resulting RNA was digested with 2 U of DNAse I for 30 min at 37 °C and further purified using RNA clean & concentrator 5 columns (Zymo research). About 1.5 μg of total RNA was retrotranscribed with hexamers using Superscript VI retrotranscriptase (Invitrogen) ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 40–60 minutes and demethylated using rtStar tRNA-optimized First strand cDNA synthesis kit following manufacturer’s protocol with intervening RNA cleaning steps (Zymo RNA Clean and Concentration Kit). For the annealing reaction ...
-
bioRxiv - Genomics 2023Quote: ... Samples were bisulfite converted after size selection using EZ DNA Methylation-Gold Kit or EZ DNA Methylation-Lightning Kit (Zymo cat#D5005, cat#D5030) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... DNA from the blood was extracted using the Machery Nagel NucleoMag® Blood 200 μL extraction kit (cat, 744501) and 500 ng total DNA was bisulfite modified using the EZ-96 DNA Methylation-Lightning kit (Zymo Research Corp, cat, D5047). 8 μl of modified DNA was subjected to methylation analysis on the Illumina Infinium MethylationEPIC BeadChip (Illumina ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA was subsequently purified with the Direct-zol kit (Zymo). RNA was then precipitated overnight and resuspended after ethanol washes in 5 uL 10 mM Tris HCl pH 8.0 ...
-
bioRxiv - Cell Biology 2020Quote: ... Total RNA was isolated (Direct-zol RNA kit, Zymo Research) and quality validated on the Agilent 2200 TapeStation ...
-
bioRxiv - Genetics 2021Quote: ... and purified using the DNA Clean and Concentrator kit (Zymo). sgRNAs or crRNAs were transcribed using the HiScribe T7 High Yield RNA Synthesis kit (NEB ...
-
bioRxiv - Genetics 2021Quote: ... RNA was extracted using the extraction RNA kit (Zymo research). Leaf RNA was prepared as above except that leaves were grinded in Trizol with one metal bead.
-
bioRxiv - Genomics 2021Quote: ... The Zymo EZ DNA Methylation-Gold Kit (D5005, Zymo Research) was used for bisulfite treatment ...