Labshake search
Citations for Zymo Research :
101 - 150 of 159 citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI, USA], 2 U/mL zymolyase [Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... Filters were then transferred into a 1.5 ml tube and the filter-associated cells were resuspended by adding 1 ml 1X PBS + 0.05% Triton X-100 detergent and agitating the tubes at high speed for 2 minutes using a Genie Disruptor (Zymo). This suspension as well as the inoculum were diluted ...
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bioRxiv - Microbiology 2019Quote: ... To determine if the mock community amplicon library compositions produced by the our 2-Step library preparation and HiSeq sequencing methods were within the same variation observed by Zymo Research ...
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bioRxiv - Neuroscience 2020Quote: RNA was extracted from lumbar DRGs 2-5 following the manufacturer’s protocol with a Direct-zol RNA Miniprep Plus kit (Zymo R2071). RNA libraries were prepared and sequenced by the UTSW Microarray core facility on an Illumina NextSeq SE-75 sequencer at 40 million reads/sample ...
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bioRxiv - Microbiology 2020Quote: ... collect ~5 roots and fully submerge in a pre-labelled 2 mL microcentrifuge tube filled with DNA/RNA Shield (Zymo Research ...
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bioRxiv - Genomics 2021Quote: ... RNA was extracted from inactivated swab samples using the Quick DNA/RNA Viral Magbead kit from step 2 of the DNA/RNA purification protocol (Zymo) (files.zymoresearch.com/protocols/_r2140_r2141_quick-dna-rna_viral_magbead.pdf).
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bioRxiv - Developmental Biology 2023Quote: DNA was extracted from fin clips stored in 100% ethanol for <2 weeks using the PCR biosystems rapid extract lysis kit or Quick-DNATM Miniprep Plus kit by Zymo research ...
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bioRxiv - Microbiology 2023Quote: ... tissue was scraped from each fragment (avoiding the apical tip) and added directly to 2 mL tubes containing 0.5ml DNA/RNA shield (Zymo Research) and Lysing Matrix A (MP Biomedicals ...
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bioRxiv - Genomics 2023Quote: ... at 37 °C for 2 hours and purified using Zymo ssDNA/RNA clean & concentrator kit following manufacturer’s instructions (Zymo D7011). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products with 150-400 bp were then cut and purified with 2% agarose gel and column (Zymo research, D4008). Finally ...
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bioRxiv - Microbiology 2023Quote: ... two agar plugs were placed in a 2 mL eppendorf tube with 500 μL of chloroform and agitated on a Genie Disruptor (Zymo) for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... two agar plugs were placed in a 2 mL eppendorf tube with 500 μL of chloroform and agitated on a Genie Disruptor (Zymo) for 2 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were resolved on 2% agarose gels followed by extraction and purification of candidate DNA amplicons using Zymoclean Gel DNA Recovery Kits (Zymo Research). Purified PCR products were cloned using the TOPO TA cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA (gDNA) was isolated from a saturated overnight culture (approximately 2 x 108 cells) using YeaStar Genomic DNA Kit (Zymo Research). Genomic DNA (125 µL at 15 ng µL−1 in ultrapure water ...
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bioRxiv - Plant Biology 2019Quote: ... RNA was isolated from Tak-1 and Tak-2 whole-plant material using the Direct-Zol RNA Mini-Preparation Kit (Zymo Research). cDNA synthesis was carried out using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The Variovorax CL14 genes contained in Vectors 1 and 2 were inferred by isolating plasmid from these clones using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research) and Sanger sequencing the insert ends using primers JMC247 and JMC270 (Supplementary Table 19) ...
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bioRxiv - Microbiology 2020Quote: ... Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e. 1X PBS, pH 7.2 or Zymo DNA/RNA shield). Swabs in PBS were placed on ice and sent for culturing while the other swabs were transported back at room temperature to the laboratory and stored at -80°C ...
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bioRxiv - Cell Biology 2021Quote: ... Spheroplasting of harvested yeast cells was performed for 2 minutes in lysis buffer (1 M sorbitol 100 mM EDTA, 0.1% β-mercaptoethanol) containing 25 units of zymolyase (Zymo Research). RNA extraction was performed with an RNeasy kit (QIAGEN ...
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bioRxiv - Genetics 2021Quote: DNA samples ranging from 500 ng-2 μg were digested to free nucleosides using 5-15 U of DNA Degradase Plus (Zymo Research) in 25 μl reactions incubated for 2 hrs at 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Amplicons were excised from a 2 % agarose gel (200-800 bp fragments were excised) and purified using the Gel DNA recovery kit (Zymo Research). Library concentrations were measured with qPCR according to Illumina’s Sequencing Library qPCR Quantification Guide ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Products were loaded on a 2 % agarose gel and the appropriate bands were excised and cleaned up with the Zymoclean Gel DNA Recovery Kit (Zymo Research). All purified amplicons were shipped to Macrogen Europe B.V ...
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bioRxiv - Genomics 2021Quote: ... Chronotype was assessed by computing mid-sleep time on free days (MSF).2 Participants’ stool samples were collected using DNA/RNA Shield Fecal Collection tubes (Zymo research). Sample DNA extraction was performed using the PureLink Microbiome DNA Purification Kit (Invitrogen ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 300µm diameter) follwing a Proteinase K digestion (at 55°C for 2 hours) using a Quick-RNA MicroPrep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: RNA was extracted from pellets of ∼2 million iPSCs or ∼6 million neurons immediately after harvesting with the Quick-RNA Miniprep Kit (Zymo R1054) following manufacturer’s instructions except for two alterations ...
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bioRxiv - Physiology 2022Quote: Total RNA was extracted from larval fat bodies (2-5 pooled/sample) or larval brains (5 pooled/sample) using the Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
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bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
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bioRxiv - Bioengineering 2023Quote: The 2 μL of EVs were incubated at room temperature for 15 min with 1 U of DNase I (Zymo Research) and 1x DNA Digestion Buffer ...
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bioRxiv - Microbiology 2023Quote: ... the reaction was prepared in a volume of 20 µL consisting in 2 µL of the template DNA and 18 µL of Femto Bacterial qPCR Premix (Femto Bacterial DNA Quantification Kit, Zymo Research). The PCR reaction was performed using a 7500 Fast Real-Time PCR System (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 2-5 x107 cells grown in different nutrients (see above) with the YeaStar RNA Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... before being spun down (10k rpm, 2 minutes) and resuspended in 80 μL of digestion buffer (included in the YeaStar RNA Kit, Zymo Research). Cells were then stored on ice until ready for RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... nojo-1 and nojo-2 complete roots of plants growing 12 DAS using Quick-RNA Miniprep (Zymo Research, Irvine, CA, USA). Complementary DNA was generated using the SuperScriptTMII Reverse Transcriptase (InvitrogenTM ...
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bioRxiv - Systems Biology 2023Quote: ... Pools 4-8 from experimental replicate #2 were resuspended in 100 µL spheroplast digest buffer with 2.5U/mL Zymolyase T100 (Zymo Research #E1005) and incubated at 37°C for 20 minutes ...
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bioRxiv - Genomics 2024Quote: Isolates for sequencing were cherrypicked using an Opentrons OT-2 Liquid Handler and DNA extraction was then performed using the Quick-DNA Fecal/Soil Microbe Kit (Zymo Research) on a KingFisher Flex instrument (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... and sorted into 384-well plates preloaded with 2μl of digestion buffer for snmC-seq215 (20 mL digestion buffer consists of 10 mL M-digestion buffer (2×, Zymo D5021-9), 1 ml Proteinase K (20 mg ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from three biological replicates of E/I networks composed of Ngn2 #2 and Ascl1 #1 (DIV 49) with the Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cultured neurons at 15 DIV were left untreated or treated for 48 h with 2 μM TTX and then processed for total RNA extraction using the kit Direct-zol™ RNA MicroPrep (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... C-SCCE0625), and ScriptCap 2′-O-Methyltransferase (Cellscript, C- SCMT0625) and purified by a Direct-zol RNA Microprep Kit (Zymo Research, D2060). The radiolabeled TOP RNAs (4 pmol ...
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bioRxiv - Microbiology 2019Quote: ... and a mock microbial community DNA (8 bacterial + 2 yeasts species mixed in defined proportions) (ZymoBIOMICS Microbial Community DNA standard, Zymo Research, USA) were included in the experiments ...
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bioRxiv - Immunology 2021Quote: ... followed by a final clean-up step by agarose-gel extraction (2% (w/v) gel) with Zymoclean Gel DNA Recovery kit (Zymo Research D4001). Yeast scFv display libraries were generated using the amplified VH and VL DNA libraries from above and the pYD1 yeast surface display vector (Addgene plasmid #73447 ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified cell lysates (2×108 cells) were used to purify total RNA using the Direct-zol RNA MiniPrep kit (Zymo Research, USA). One microgram of total RNA after Turbo DNase I (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from HL-7702 cell lines and 2 μg DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (ZYMO Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... cucullus vegetative cells (300,000 cells) and 2-week-old mature cysts (3,000,000 cells) using the Direct-zol RNA purification system (Zymo Research Crop., California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... we prepared a series of 8 serial dilutions of a known mock microbial community consisting of 8 gram positive and negative bacteria and 2 yeast (Zymo Research, catalog # D6300), as previously described (31) ...
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bioRxiv - Microbiology 2022Quote: ... 2 extracted with tissue samples and 1 run with water samples) and 2 samples of DNA from mock bacterial communities (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Irvine CA) were prepared and sequenced ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was extracted from 2-10 mL of stationary cultures with the Zymo Research Midiprep gDNA kit (Zymo Research EUROPE GmbH, Germany). Library preparation and sequencing were performed at Novogene (UK ...
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bioRxiv - Genomics 2023Quote: ... Purification was performed according to manufacturer’s instructions and the converted DNA was then eluted in 32.5 µl nuclease-free water (17 µl × 2 times) through a column from the DNA Clean & Concentrator kit (Zymo Research, cat. no. D4003).
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bioRxiv - Cancer Biology 2022Quote: ... The outer PCR products amplified using primer #1 and #2 were purified and concentrated using the DNA Clean and Concentrator 5 kit (Zymo Research, Orange, CA) and eluted in 20ul of TE buffer ...