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Citations for Zymo Research :
101 - 150 of 166 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The cDNA was purified in 10 μl water using the Oligo Clean and Concentrator™ kit (Zymo). Next ...
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bioRxiv - Immunology 2022Quote: ... Resulting cDNAs were pooled and purified by the DNA Clean and Concentrator TM-5 kit (Zymo research) and treated with Exonuclease I (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... mitochondria and mitoplasts isolated from magnetic-bead IP (40 ng) methods were converted to cDNA libraries (Zymo-Seq RiboFree Total RNA #R300 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... cDNA synthesis was immediately followed by a cleanup step using a DNA Clean & Concentrator Kit (Zymo Clean).
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bioRxiv - Microbiology 2024Quote: ... Japan) and cDNA was synthesized with the Direct-zol™ RNA Miniprep (Zymo Research, Orange, CA, USA) and ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo ...
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bioRxiv - Genomics 2020Quote: ... flash frozen in liquid nitrogen and stored at -80 °C for further usage or RNA was extracted using the Quick-RNA FFPE Miniprep kit (Zymo Research, Cat. N: R1008) with Zymo-Spin IC Columns (Zymo Research ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted in carbon free N−C− minimal medium and spread on LB agar plates using Rattler Plating Beads (Zymo Research, Irvine, CA, USA) with a target density of 200 colonies per plate ...
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bioRxiv - Bioengineering 2024Quote: Cell number was directly assessed as a function of total isolated DNA at experimental Days 1 and 7 using a Zymo Quick-DNA Microprep Plus Kit (n=5, Zymo Research Crop, Irvine, CA). A standard curve at experimental Day 0 was generated using known quantities of hMSCs and following the “Biological Fluids & Cells” protocol as directed ...
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bioRxiv - Molecular Biology 2020Quote: ... Universally methylated mouse DNA standard (cat. no. D5012, Zymo Research) and Mlh1−/− tissues were used as Mlh1+/+ and Mlh1−/− allele controls ...
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bioRxiv - Microbiology 2024Quote: The DNA of the ancestor strains and their clones derived after repeated treatment with phage NP3 or M32 and the respective untreated clones after 72 h growth were isolated using the ZymoBIOMICS DNA Mircoprep Kit (Zymo Research Europe GmbH ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from whole-tissue (50 ng) and isolated mitochondria (10 ng) were converted to cDNA libraries (Zymo-Seq RiboFree Total RNA #R300 ...
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bioRxiv - Microbiology 2024Quote: ... followed by neutralization with HCl (5M) and cDNA purification using the Zymo Oligo Clean & Concentrator kit (Zymo Research). The sample was eluted with water and vacuum concentrated to 5 ul ...
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bioRxiv - Molecular Biology 2021Quote: High-molecular-weight genomic DNA from HAP1 Δ72 and HepG2 Δ15 deletion clone was extracted using Quick-DNA kit (Zymo Research) and shipped to Samplix Services (Denmark ...
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bioRxiv - Microbiology 2020Quote: ... The Variovorax CL14 genes contained in Vectors 1 and 2 were inferred by isolating plasmid from these clones using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research) and Sanger sequencing the insert ends using primers JMC247 and JMC270 (Supplementary Table 19) ...
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bioRxiv - Genetics 2021Quote: ... Isolation of the plasmid DNA from bacterial clones was conducted using Zymo or Qiagen mini prep and maxi prep kits (Qiagen #27104, Zymo #D4208T)
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bioRxiv - Microbiology 2024Quote: RNA extractions and cDNA reactions were performed as described previously(1) using Direct-zol RNA miniprep kit (Zymo Reserach) and RevertAid RT Kit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was extracted from HBEC controls and various HBEC derivative cells (three different clones for each group) using a Quick-DNA Miniprep Plus kit (Zymo Research, D4070). DNA samples were submitted to the Van Andel Institute’s Genomics Core (Controls ...
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bioRxiv - Molecular Biology 2020Quote: ... Universally methylated mouse DNA standard (cat. no. D5012, Zymo Research, Irvine, CA) and genomic DNA extracted from normal spleen was used as positive and negative controls ...
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bioRxiv - Genetics 2021Quote: IL2 and CD20 constructs were created through PCR amplification from cDNA and genomic DNA respectively.PCR products were purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research) and cloned into the pEntryD-Topo vector ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting double-stranded cDNA was purified with 1.8X volumes of Select-a-Size DNA Clean and Concentrator MagBeads (Zymo Research).
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bioRxiv - Pathology 2022Quote: RNA was extracted and cDNA was synthesized according to the manufactures’ manuals of Quick-RNA Miniprep kit (Zymo Research, R1054) and High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... decorated cDNA from multiple samples were pooled together and purified using the DNA Clean & concentrator-5 Kit (Zymo research, #D4014). After elution with 20 µl of nuclease free water ...
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bioRxiv - Developmental Biology 2021Quote: cDNA preparation and preamplification: RNA isolation of digested samples was performed using the Quick-RNA MicroPrep kit (Zymo Research, Cat #: R1050). In-column DNAse-I treatment was performed for trace DNA removal before eluting RNA ...
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bioRxiv - Neuroscience 2021Quote: ... we used Q5 hot-start high-fidelity DNA polymerase to amplify the transcripts of each candidate gene from complementary DNA (cDNA) synthesized using the total RNA of w1118 fly heads (extracted using MiniPrep kit; Zymo Research). For each candidate gene ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR products containing linked TCRα:β cDNA amplicons were purified using a DNA Clean & Concentrator Kit (D4033, Zymo Research, Irvine, CA).
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bioRxiv - Plant Biology 2022Quote: ... YES3-Gate-OPT3 construct or YES3-Gate lacking the cDNA insert were transformed into appropriate yeast line using the Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformants were selected for uracil prototrophy on YNB medium containing 6.7% (w/v ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were run on an UltraPure Agarose (Thermo Fischer Scientific) gel and cDNA fragments larger than 500 nt were purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo Research). The library was prepared using the ONT 1D kit (SQK-LSK108 ...
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Modifications to the published method are the following: 1) extraction of RNA prior to cDNA reverse transcription with the Zymo Direct-zol-96 RNA isolation kit (Zymo, #R0256) 2 ...
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bioRxiv - Bioengineering 2022Quote: cDNA samples were again purified via column purification using a Zymo Research DNA Clean and Concentrator-25 kit (Zymo, Irvine, CA). Select 200-300 bp regions were then amplified using specific primers designed against various hotspot regions found in the patient-sample derived consensus sequence (Supplementary Table 5) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from whole-tissue (50 ng) and isolated mitochondria (10 ng) were converted to cDNA libraries (Zymo-Seq RiboFree Total RNA #R300, Zymo Research). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... mitochondria and mitoplasts isolated from magnetic-bead IP (40 ng) methods were converted to cDNA libraries (Zymo-Seq RiboFree Total RNA #R300, Zymo Research) then underwent whole transcriptome RNA sequencing as described above.
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bioRxiv - Cell Biology 2020Quote: ... and primary mouse keratinocytes was isolated using Direct-zol RNA Microprep Kits (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: RNA from mouse lungs was extracted using RNA Miniprep Plus Kit (Zymo Research). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleaned pools of samples were gel purified to remove excess primer and eluted cDNA was cleaned through Zymo DNA Clean and Concentrator kit (Zymo Research #D4014).
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bioRxiv - Molecular Biology 2024Quote: ... The post-RT droplets were broken with 10% perfluorooctanol and supernatant containing barcoded-cDNA was collected by passing through a filter column (Zymo, C1004-250) at 1000g for 1 min.
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bioRxiv - Biophysics 2023Quote: ... The cDNA was size selected using denatured polyacrylamide gel electrophoresis followed by dPAGE purification (small-RNA PAGE Recovery Kit, Zymo Research #R1070). The cDNA was then amplified using Q5-Ultra II DNA polymerase (New England Biolabs #M0544 ...
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bioRxiv - Cell Biology 2023Quote: gDNA was isolated from mouse gastrocnemius using Quick-DNA Microprep Plus Kit (Zymo D4074). gDNA was amplified using CloneAmp™ HiFi PCR Premix (Takara 639298 ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated from 5µl of mouse serum using the RNA Clean & Concentrator -5 (Zymo) or High Pure Viral Nucleic Acid kit (Roche ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was isolated from mouse kidneys using Direct-zolTM RNA MiniPrep Plus (Zymo Research). 1 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 40–60 minutes and demethylated using rtStar tRNA-optimized First strand cDNA synthesis kit following manufacturer’s protocol with intervening RNA cleaning steps (Zymo RNA Clean and Concentration Kit). For the annealing reaction ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from mouse kidneys using Direct-zol™ RNA MiniPrep Plus (Zymo Research). 2 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research), and all of them were assessed for purity and quantity using a Nanodrop 1000 spectrophotometer ...
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bioRxiv - Molecular Biology 2021Quote: ... pLXSP-16E7 or control vector infected primary mouse keratinocytes with the Quick-RNA Miniprep kit (Zymo Research). cDNA was synthesized with the Quantinova Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Genomics 2020Quote: Total RNA was isolated from mouse cochlea sensory epithelia using the Direct-zolTM RNA MiniPrep (Zymo Research). The RNA was quantified using a Nano-drop® (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from mouse hearts using either the Direct-zol RNA Miniprep kit (Zymo, cat# R2051) or the RNeasy Fibrous Tissue Mini Kit (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from DIV12 infected mouse cortical neurons using the Direct-zol RNA microprep (Zymo). Ten µg were reverse-transcribed into cDNA by Maxima First Strand cDNA Synthesis Kit (Thermofischer) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse hippocampus with the Quick-RNA Miniprep Plus Kit (Zymo Research, R1058) and RNA integrity was determined using Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from mouse tissues using the Quick-RNA MiniPrep kit (#R1055, Zymo Research, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA (400 ng) from mouse tail was bisulphite converted with the EZ DNA Methylation-Lightning kit (Zymo Research ...