Labshake search
Citations for Zymo Research :
1251 - 1300 of 1328 citations for 1 4 Dichloro 2 5 bis dichloromethyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... a small piece of gel (∼100 μl volume) was dissolved in 400 μl TRI-reagent (Zymo Research, R2050-1-200) and the RNA was purified using an RNA isolation kit (Zymo Research ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
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bioRxiv - Genomics 2023Quote: ... the cells were pelleted and resuspended at 1x106 cells per 300 ul of RNA lysis buffer (Zymo, #R1060-1-100). Cells were pipette mixed and frozen at -80 until RNA isolation was performed ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were then reverse-crosslinked overnight in elution buffer (1 % SDS, 0.1 M NaHCO3) and purified using the ChIP DNA Clean & Concentrator kit (Zymo Research) following manufacturer instructions ...
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bioRxiv - Zoology 2024Quote: ... The cDNA to be used as a template for PCR amplification was obtained from a mix of ten 1 to 25 days-old CSFB adults by RNA extraction using Quick tissue kit (Zymo) and reverse transcription using the LunaScript® RT SuperMix Kit ...
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bioRxiv - Microbiology 2024Quote: ... coli cells co-expressing FaRel2-FLAG3 with either ATfaRel2 or PaSpoSSU5 were deacylated under 100 μL of 100 mM CHES-NaOH pH 9.0 for 1 hour at 37°C and purified by Oligo Clean & Concentrator (Zymo Research). Before adapter ligation ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 mg of genomic DNA was treated with sodium bisulfite using EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 1 ug of genomic DNA was bisulfite (BS)-converted using the EZ DNA Methylation Gold Kit (Zymo Research Cat# D5006) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Linear PCR products were purified from agarose gel (1%) after electrophoresis with a Zymoclean-Gel DNA Recovery kit (Zymo Research), circularized using DNA Ligation Kit
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were isolated from fully grown cultures (1 mL) using a Zymoprep™ Yeast Plasmid Miniprep II kit (Zymo Research) according to the manufacturer’s protocol and the remaining culture was propagated to start the next screening round.
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bioRxiv - Cell Biology 2024Quote: ... The streptavidin beads were then eluted by incubation in 1 mL TRIzol at 25°C for 10 minutes and the glycoRNA was extracted and purified by Zymo C column ...
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bioRxiv - Biochemistry 2022Quote: ... These sorted libraries were grown for 1 day at 30°C shaking in SD-CAA media and then 300 µl of the cultures were miniprepped (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... DNA was then extracted from approximately 1 ml of the red blood cell pellets using Zymo Bead Genomic DNA kit (Zymo Research) following the manufacturer’s procedures ...
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bioRxiv - Genomics 2020Quote: ... We plated 1% of the library to estimate complexity and grew the rest of the sample and then midi prepped (Zymo D4200) the resulting library.
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bioRxiv - Biochemistry 2021Quote: ... The PCR products were subjected to 1% agarose gel electrophoresis and they were purified using a Zymogen gel DNA recovery kit (Zymo Research). The assembly reactions were transformed to chemically competent E ...
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bioRxiv - Cancer Biology 2021Quote: Genomic DNA was extracted from GBM-DCs and TRA-1-81+/SSEA4+ ic-GSCs using the Quick-DNA Microprep Kit (D-3020, Zymo Research). Bisulfite conversion ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was extracted from 1 ml breastmilk using Quick-DNA Fungal/Bacterial extraction kit following the manufacturer’s instructions (Zymo Research, USA). Extraction kits were purchased separately for Batch 1 and Batch 2 ...
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bioRxiv - Plant Biology 2022Quote: ... A final concentration of 0.1 ng/µL was employed for both the Femto Fungal DNA Quantification Kit and Femto Bacterial DNA Quantification Kit (Zymo Research) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from CEM-SS or Tet-inducible HTLV-1 Tax CEM-SS cells that were infected with DNaseI-treated WT or IN- HIV-1 at 2dpi using a DNA Miniprep Plus kit (Zymo Research). Purified DNA was then digested with MscI ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were incubated for 1 hour at 37 °C and DNA was purified using the DNA Clean & Concentrator-25 kit (Zymo Research) as described by the manufacturer.
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bioRxiv - Plant Biology 2020Quote: ... ToMVMP-ToBRFV or ToMVMP-ToBRFV(1-216) were linearized with SmaI and cleaned by Gel extraction kit (Zymo Research, ZR-D4002). In vitro transcription using the mMESSAGE mMACHINE™ T7 kit (Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... 100 mg of thawed cell pellets were washed three times in 1 mL deionized water and extracted using Quick-DNA Fungal/Bacterial Miniprep kit (Zymo Research). A total of 2.4 μg of purified gDNA was fragmented by sonication to average size of ~350 bp in four separate aliquots using a Diagenode Picoruptor ...
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bioRxiv - Genomics 2022Quote: ... A 2-cm-long piece of each cotton strip was cut off and transferred into a ZR BashingBead Lysis Tube (0.1 & 0.5 mm) pre-filled with 1 mL of DNA/RNAShield (Zymo Research, Freiburg, Germany) using sterile laboratory gloves ...
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bioRxiv - Microbiology 2022Quote: ~1 kb products from the Pan-Entamoeba PCR above were gel purified using a Zymoclean Gel DNA Recovery Kit (Zymo Research) and submitted to the UW-Madison Biotechnology Center for Sanger Sequencing using the amplification primers described above ...
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bioRxiv - Cancer Biology 2023Quote: ... Modifications to the published method are the following: 1) extraction of RNA prior to cDNA reverse transcription with the Zymo Direct-zol-96 RNA isolation kit (Zymo, #R0256) 2 ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× RNase H buffer overnight at 37 °C before immunoprecipitation and recovered using the Oligo Clean & Concentrator kit (Zymo Research). For m1A-DIP ...
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bioRxiv - Bioengineering 2024Quote: Up to 1 × 108 yeast cells from a growing culture were taken to perform a zymoprep using the ZymoprepII kit (D2004, Zymo Research). The TRACeR sequences were amplified from the extracted plasmids and the adapter sequences were added by PCR ...
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bioRxiv - Genomics 2023Quote: ... the supernatant was transferred to a deep 96-well plate and mixed with 400ul of genomic lysis buffer (Zymo, #D3004-1). The mix was stored at -20C to await further processing ...
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bioRxiv - Molecular Biology 2023Quote: ... aqueous phase containing cfRNAs was mixed with 1 volume of ethanol (95-100%) and then added to the Zymo-Spin column (Zymo, R1016) for RNA binding ...
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bioRxiv - Microbiology 2023Quote: Cells were infected with 1 MOI of TB40/EGFP and DNA and RNA was isolated using Quick-DNA/RNA miniprep kit (Zymo Research) from 0–72 hpi ...
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bioRxiv - Genomics 2023Quote: Total RNA (∼1 µg) was used to make RNA-seq libraries following the Zymo-Seq Ribo-free Total RNA Library Kit (Zymo Research) instructions with dual indexing ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from each well of a 6-well plate containing ES or DE cells using 1 mL Tripure and the Direct-Zol RNA Extraction kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Constructs were circularized with vector backbone by Gibson assembly and introduced to XL-1 Blue Mix and Go chemical competent cells (Zymo Research), miniprepped (Qiagen) ...
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bioRxiv - Bioengineering 2023Quote: ... The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research) supplemented with RNase A (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: BMDMs (1 × 106) were treated as indicated and RNA was purified using Direct-zol RNA Miniprep (Zymo Research Corporation, Cat# R2053). Samples were submitted to Azenta Life Sciences for standard RNA-seq processing ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from 1*106 WT or sorted SLAMF6-KO NK-92 cells using an RNA Micro Kit (Zymo Research). After PolyA selection libraries were prepared and lllumina sequencing of 20-30 million paired-end reads performed ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was isolated from 1-day old zebrafish embryos with TRI Reagent using Direct-zolTM RNA MicroPrep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Following MASC PCR validation using the MASC PCR primer set described in Supplementary Data 1 and BAC extraction using the ZR BAC DNA Miniprep Kit (Zymo Research) based on the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was prepared from 1×106 cells of HAP1 and HELLS KO using Direct-zol RNA Microprep kit (Zymo Research). The RNA was checked for quality using the Agilent BioAnalzyer 2100 ...
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bioRxiv - Immunology 2024Quote: Interior nasal turbinate swabs (herein referred to as nasal swabs) were collected and stored in 1 mL of Zymo-DNA/RNA shield reagent (Zymo Research), before RNA was extracted twice in parallel from 250 μL of sample and purified with the KingFisher Flex sample purification system (ThermoFisher ...
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bioRxiv - Genetics 2024Quote: ... followed by resuspension of ∼200k to 1 million cells in 300ul RNA Lysis Buffer from the Quick-RNA Miniprep Kit (Zymo R1055) and stored in −70deg ...
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bioRxiv - Genomics 2024Quote: gDNA was isolated from ∼25 mg of frozen tissue or ∼1 × 106 HepG2 cells suspended in 200 µL of PBS using the Quick-DNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocol with an overnight (for frozen tissue ...
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bioRxiv - Bioengineering 2024Quote: ... the collected yeast cells were grown to saturation and 1 ml of saturated culture was removed for DNA extraction using the Zymoprep yeast Plasmid Miniprep II (Zymo Research) kit according to manufacturer protocols (using 10 µl zymolyase) ...
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bioRxiv - Genetics 2021Quote: DNA bisulfite conversion was performed directly starting from cell pellets (a maximum of 1×105 cells per sample) using the EZ DNA Methylation-Direct kit (Zymo Research #D5021) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The corresponding amount of polyA RNA were incubated with 1X fragmentation buffer at 95 °C for 1 minute to generate ~300 nt of RNA fragment followed by column purification (Zymo Research, R1016) as described before [23] ...
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bioRxiv - Physiology 2022Quote: Total RNA from adipose tissues (experiment 1) was extracted using the Direct-zol™ RNA MiniPrep Plus kit protocol (Zymo research, #R2071). RNA was reverse-transcribed using the SuperScript™ III Reverse Transcriptase Kit (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
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bioRxiv - Genetics 2020Quote: ... DNA was digested in columns by adding a mixture of 5 μl RNA Lysis Buffer 1 from the kit andl DNase I and 75 μl RNA Lysis Buffer 1 from the kit andl DNA Digestion Buffer from DNase I Set kit (Zymo Research E1010) followed by a 15-minute incubation at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Genomic DNA (1 μg) was treated with sodium bisulfite using the EZ DNA Methylation Gold Kit per manufacturer’s instructions (Zymo Research; Irvina, CA). Bisulfite converted DNA (∼20 ng ...