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Citations for Zymo Research :
1151 - 1194 of 1194 citations for 6 1 BROMO ETHYL 4 CHLORO 5 FLUOROPYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... followed by resuspension of ∼200k to 1 million cells in 300ul RNA Lysis Buffer from the Quick-RNA Miniprep Kit (Zymo R1055) and stored in −70deg ...
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bioRxiv - Genomics 2024Quote: gDNA was isolated from ∼25 mg of frozen tissue or ∼1 × 106 HepG2 cells suspended in 200 µL of PBS using the Quick-DNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocol with an overnight (for frozen tissue ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from 1*106 WT or sorted SLAMF6-KO NK-92 cells using an RNA Micro Kit (Zymo Research). After PolyA selection libraries were prepared and lllumina sequencing of 20-30 million paired-end reads performed ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was isolated from 1-day old zebrafish embryos with TRI Reagent using Direct-zolTM RNA MicroPrep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Following MASC PCR validation using the MASC PCR primer set described in Supplementary Data 1 and BAC extraction using the ZR BAC DNA Miniprep Kit (Zymo Research) based on the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: DNA bisulfite conversion was performed directly starting from cell pellets (a maximum of 1×105 cells per sample) using the EZ DNA Methylation-Direct kit (Zymo Research #D5021) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... PCR products were applied to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The corresponding amount of polyA RNA were incubated with 1X fragmentation buffer at 95 °C for 1 minute to generate ~300 nt of RNA fragment followed by column purification (Zymo Research, R1016) as described before [23] ...
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bioRxiv - Genomics 2019Quote: ... or by sodium bisulfite using two commercially available kits: BS (1)EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA Research) and BS (2 ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from three biological replicates of E/I networks composed of Ngn2 #2 and Ascl1 #1 (DIV 49) with the Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Total RNA from adipose tissues (experiment 1) was extracted using the Direct-zol™ RNA MiniPrep Plus kit protocol (Zymo research, #R2071). RNA was reverse-transcribed using the SuperScript™ III Reverse Transcriptase Kit (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
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bioRxiv - Genetics 2020Quote: ... DNA was digested in columns by adding a mixture of 5 μl RNA Lysis Buffer 1 from the kit andl DNase I and 75 μl RNA Lysis Buffer 1 from the kit andl DNA Digestion Buffer from DNase I Set kit (Zymo Research E1010) followed by a 15-minute incubation at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Genomic DNA (1 μg) was treated with sodium bisulfite using the EZ DNA Methylation Gold Kit per manufacturer’s instructions (Zymo Research; Irvina, CA). Bisulfite converted DNA (∼20 ng ...
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bioRxiv - Immunology 2021Quote: ... PCR products were subjected to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: DNA bisulfite conversion was performed starting from a maximum of 1 × 105 pelleted cells per sample using the EZ DNA Methylation-Direct kit (Zymo Research #D5021), and following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The amplified promoter and coding PIGJ DNA sequence were first purified from 1% agarose gels using the ZymocleanTM Gel DNA recovery kit (Zymo Research, #D4007) and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were grown in Sc-Ura medium for 1 d and plasmid DNA was purified using Zymoprep II Yeast plasmid Miniprep kit (Zymo Research, CA). Plasmids were then transformed into E ...
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bioRxiv - Plant Biology 2023Quote: ... The adapter-ligated DNA molecules were purified using 1× Agencourt AMPure XP magnetic beads and were subjected to bisulfite conversion using ZYMO EZ DNA Methylation-Gold Kit (Zymo, Cat. #D5005). Libraries were then amplified by PCR using 20 μL of bisulfite-converted DNA molecules ...
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bioRxiv - Cell Biology 2024Quote: ... the beads were boiled in Tris-EDTA containing 1% SDS and 12% β-Mercaptoethanol before RNA was extracted using a Direct-zol RNA Microprep kit (Zymo Research). The extracted RNA was then prepared for RT- qPCR analysis to examine RB1 ...
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bioRxiv - Immunology 2024Quote: ... coli were resuspended in 1 ml of TRIzol and their RNA was purified using Quick-RNA DirectZol columns ((#ZR2072 Zymo Research Ozyme) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... WT and Rnlsmut NIT-1 cells were used for RNA isolation using Zymo Quick-RNA miniprep plus kit (Zymo Research cat. R1058). Novogene Corporation Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared supernatant was discarded and the concentrated high-speed pellet (“100,000 x g pellet”) was lysed directly in 300 μl of TRI reagent (Zymo Research, R2050-1-200). RNA extraction was performed according to instructions for the Direct-zol RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Immunology 2021Quote: ... mice were euthanized with isoflurane overdose and one lobe of lung tissue was collected in an Omni Bead ruptor tube filled with Tri Reagent (Zymo, #R2050-1-200). Tissue was homogenized using an Omni Bead Ruptor 24 (5.15 ms ...
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bioRxiv - Molecular Biology 2022Quote: ... the aqueous phase was diluted back in TRizol reagent (1:3) to proceed with RNA purification by zymodirect RNA kit (Zymo research, cat. R2051). The quality of RNA samples was assessed using Agilent 2200 TapeStation (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... 2 extracted with tissue samples and 1 run with water samples) and 2 samples of DNA from mock bacterial communities (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Irvine CA) were prepared and sequenced ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research, Irvine, CA). The purified DNA fragments were then submitted for Sanger sequencing (GENEWIZ ...
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bioRxiv - Plant Biology 2022Quote: ... Fragments were resolved with gel electrophoresis (1% agarose in 1x TAE buffer) and excised using the Zymoclean Gel DNA Recovery Kit (Zymo Research; Ref.: D4008) for elution of high-concentration ultra-pure DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Two-third of final flag elute was taken for DNase treatment using NEB DNase 1 (M0303) and RNA was purified using RNA Clean and Concentrator Kit (Zymo Research, R1015/R1017). The final RNA concentration was determined using Qubit RNA high Sensitivity Assay Kit and 100ng of RIP-purified RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α λpir (Table 1) was prepared using the commercially available Mix and Go™ transformation kit (Zymo Research International, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of genomic DNA per sample was subjected to bisulfite conversion using the EZ DNA Methylation Kit (Zymo research Cat. no. D5001) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified samples were run on a 2% agarose gel and fragments of the correct size were purified using ADB Agarose Dissolving Buffer (Zymo D4001-1-100) and UPrep Micro Spin Columns (Genesee Scientific 88– 343) ...
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bioRxiv - Evolutionary Biology 2024Quote: PCR products were visualized by agarose gel (1%) electrophoresis then extracted and purified using Zymoclean Gel DNA Recovery Kit (Zymo Research Lot. #213587) following manufacturer protocols ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and 3 swabs (2 for metagenomic DNA isolation, 1 for storage) moistened with DNA/RNA shield (Zymo Research, Cat. No. ZYR.R1100-250). Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e ...
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bioRxiv - Molecular Biology 2020Quote: ... Prior to extraction the samples were bead-beaten for 1 minute at 1600 RPM using ZR Bashing Bead Lysis matrix (S6012 - Zymo Research, Irvine, CA, USA). After bead beating ...
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bioRxiv - Microbiology 2021Quote: ... were then homogenised in 200 µl nuclease-free water using a tube pestle and host cells lysed by addition of 1 ml of host depletion solution (Zymo Research, Irvine, CA, USA) with a 15 min incubation at room temperature with end over end mixing ...
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bioRxiv - Microbiology 2021Quote: ... were drawn from the intravenous line at 1- or 2-hour intervals and collected in DNA/RNA shield (Cat# R1150, Zymo Research, Irvine, CA, USA). All procedures were approved by SNUBH IACUC (BA1804-246/040-01).
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from 1 × 105 cells using the Quick-RNA MiniPrep kit with Zymo-Spin IIICG columns (Zymo Research, Irvine, CA USA) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... total DNA or total RNA was extracted from 1−3 × 106 cells using the Quick-DNA or Quick-RNA Miniprep Kits (Zymo Research, Irvine, CA USA) respectively ...
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bioRxiv - Genomics 2020Quote: Bisulfite conversion of 1 ug genomic DNA was performed using the EZ-96 DNA Methylation Kit (Deep Well Format) (Zymo Research; Irvine, CA, USA) according to the manufacturer’s instructions (www.zymoresearch.com) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL, Zymo-IIICG = 60μL, all from Zymo Research), and then allowed to incubate on the column at 37°C for 45 min ...
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bioRxiv - Cell Biology 2023Quote: ... Double stranded TA-bridges were synthesized annealing BM-1 with non-biotinylated oligos and DNA was purified using Genomic DNA Clean & Concentrator™ (ZYMO RESEARCH, Cat.No. D4065). 30 fmol from each biotinylated oligos were used per reaction and biotinylated Epstein-Barr nuclear antigen (EBNA ...
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bioRxiv - Physiology 2024Quote: ... Complementary DNA (cDNA) was synthesized from 1 to 2 μg of RNA using High-Capacity RNA-to-cDNA™ Kit (Zymo Research, Irvine, CA). Quantitative real-time polymerase chain reaction (qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sub pool was further amplified with lsPCR2 primers in Supplementary Table 1 by PCR amplification of 10 cycles and the library pools were gel purified (Zymo Gel DNA Recovery Kit).