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Citations for Zymo Research :
1151 - 1200 of 1344 citations for 1 5 Bis 2 2 methyl 1 oxoallyl oxy ethyl dihydrogen benzene 1 2 4 5 tetracarboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The tagmented DNA was cleaned up using Zymo DNA Clean and Concentrator-5 columns with 5X binding buffer (Zymo, cat. no. D4004). 5 μL of purified DNA out of 12.5 μL purified product was amplified in a 25 μL PCR reaction containing NEBNext PCR master mix (1X final) ...
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmentation was performed for 30 minutes at 37°Con a rotator and DNA was purified using the DNA Clean and Concentrator 5 kit (Zymo Research D4004). PCR was performed as previously described [23] using 11 total cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were treated with 0.5 mg/ml proteinase K at 65 ℃ for one hour and purified with Zymo ChIP DNA Clean Concentrator kit (Zymo Research D5205) as the manufacturer’s manual and quantified by QUBIT ...
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bioRxiv - Molecular Biology 2024Quote: ... The aqueous phase was retained for RNA purification using the Zymo RNA Clean & Concentrator-5 kit following manufacturer directions for the capture of small RNA fragments with slight modification (Zymo Research, R1013). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... 75 μl of nuclease-free water was added to the mix followed by 270 μl of RNA binding buffer (RNA Clean and Concentrator-5 column kit, Zymo Research, R1015) and 400 μl of 100% ethanol ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were purified from agarose gels using the Zymoclean® Gel DNA Recovery Kit or from PCR reactions using the DNA Clean & Concentrator®-5 DNA kit from Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted by phenol-chloroform for the ZNF469 profiling to keep the small DNA fragments and with the DNA Clean & Concentrator-5 kit (Zymo Research, D4004) for the H3K27ac profiling ...
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bioRxiv - Microbiology 2024Quote: ... was amplified using flanking primers and the resulting PCR products were purified using a DNA Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA). STm 14028s carrying the pKD46 plasmid (64 ...
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bioRxiv - Developmental Biology 2020Quote: ... Extracted DNA was treated with RNAse A (1 μL of 1 mg/mL added to samples of 100 μL for 30 minutes at room temperature) and then purified and concentrated using a DNA Clean and Concentrator-5 Kit (Zymo Research Corp., USA). The concentration of genomic DNA was measured on a QUBIT 4 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from the sorted cells as described (Hrvatin et al., 2014a) and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1013). Complementary DNA (cDNA ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed from the 0.22 μm filters (containing the 5–0.22 μm microbial size fraction) using the ZR Soil Microbe DNA MiniPrep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: IRES-Venus and human SNCA fragments were amplified by PCR (MJ Research, PTC-200 Peltier Thermal Cycler) and purified using DNA Clean & Concentrator™-5 Kit (Zymo Research, D4003) to provide 20 μl of eluted DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was isolated using phenol-chloroform extraction and then purified and concentrated using ethanol precipitation and washing on a silica column (RNA Clean & Concentrator-5, Zymo Research, Irvine, CA). Total RNA samples were reverse transcribed (ThermoFisher SuperScirpt IV Vilo) ...
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bioRxiv - Microbiology 2023Quote: ... Remaining material from the same DNA extract of subculture 19.9 was then concentrated using the DNA Clean and Concentrator-5 kit (Zymo Research; Irvine, California, U.S.A.) and used for long read sequencing ...
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bioRxiv - Systems Biology 2024Quote: ... Microbial genomic DNA was extracted from frozen feces (∼100 mg, or 5 pellets) with the ZymoBIOMICS 96 MagBead DNA Kit with lysis tubes (Zymo Research, Irvine, CA), according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was purified and the DNA oligos were removed from samples using RNA Clean & Concentrator™-5 columns with on-column DNase digest (Zymo Research, R1014) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was removed from the purified RNA samples using RNA Clean & Concentrator™-5 columns with on-column DNase digest (Zymo Research, R1014). For rRNA-depleted samples ...
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bioRxiv - Immunology 2021Quote: ... containing 1% pME and total RNA was isolated using the Quick-RNA MicroPrep Kit (Zymo Research, 11-328M). All subsequent RNA was used for reverse transcription using random hexamer and oligo dT primers with SuperScriptll (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... An aliquot of each lung homogenate (300µL) was added to 100µL of TRIReagent (Zymo Research R2050-1-200) and stored at −80°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Fragments were amplified using overhang primers (Supplementary table 1) and purified using DNA Clean & Concentrator kit (Zymo Research). The pUC19-iTol2 backbone was digested with BamHI-HF (NEB ...
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bioRxiv - Immunology 2022Quote: ... 500μL of nasal wash was added to 500μL of TRIzol reagent (Zymo Research Catalog No R2050-1-200) and the remainder of the nasal wash was stored for serological analysis ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng - 1 μg of poly(A)+ RNA was converted using the EZ RNA methylation kit (Zymo Research) with a modified high-stringency conversion condition (Sulfonation ...
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bioRxiv - Neuroscience 2022Quote: ... linear PCR products were purified from agarose gel (1%) with a Zymoclean-Gel DNA Recovery kit (Zymo Research) and circularized with DNA Ligation Kit ‘Mighty Mix’ (Takara) ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour at room temperature followed by isolation with a RNA Clean & Concentrator-25 kit (Zymo Research). 1 µg RNA was converted to ribosomal depleted cDNA libraries ready for sequencing using the TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... the PCR master mix has been treated with 0.02% (v/v) of DNAse I 1 U/mL (Zymo) at room temperature for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... After purifying both PCR products using a 1% agarose gel and a gel purification kit (Zymo, no. D4007), the two fragments were assembled using a Gibson assembly mix (NEB ...
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bioRxiv - Microbiology 2023Quote: ... 70∼80% cells were positive for Thy1.1 after sorting and lysed with TRI reagent (Zymo Research #R2050-1) for RT-qPCR.
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng-1 μg of genomic DNA was bisulfite converted using the EZ DNA Methylation-Gold kit (Zymo). Bisulfite-converted DNA was PCR amplified ...
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bioRxiv - Microbiology 2024Quote: ... RNA was isolated from ∼ 1 x 106 cells using Quick-RNATM miniprep kit (Zymo research, Cat-No.: ZR1055). cDNA was synthesized from the RNA samples using GoScript™ Reverse Transcription System (Promega ...
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bioRxiv - Genomics 2024Quote: ... Each cell pellet was re-suspended in 500µL of TRI Reagent (Zymo Research; Cat. No. R2050-1-200) and each technical duplicate was combined into 1 tube with a final volume of 1mL ...
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bioRxiv - Plant Biology 2020Quote: ... a qRT-PCR product was purified from a complete PCR reaction using the Zymo DNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA) and eluted in 10 μl of nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA extractions were performed upon 5 million trophozoites of Nf69 using Quick-DNA Miniprep Plus Kit according to manufacturer recommendations (Zymo Research, Irvine, CA, USA). In short ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The sample was then centrifuged at 2300 rpm for 5 min and the supernatant was collected and concentrated and purified using a Zymo-Spin V reservoir (Zymo Research Irvine, CA, USA) and Qiagen MinElute spin column (Qiagen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... strains were struck to synthetic defined (SD) media (CSM minus arginine, yeast nitrogen base, glucose) containing 5-Fluoroorotic Acid (5FOA) (Zymo Research, Irvine, CA, USA). to isolate cells that spontaneously lost pTC416-SEC53 ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mL of suspended biomass was collected in triplicate from the mixed tank and PBR effluent and stored in 5 mL polypropylene transport tubes (FisherbrandTM, USA) prefilled with 3 mL DNA/RNA ShieldTM (Zymo Research corp., CA, USA). The samples were briefly vortexed and stored at -20 °C until they were shipped to University at Buffalo (UB ...
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bioRxiv - Neuroscience 2023Quote: RRBS libraries were prepared from 200 ng of genomic DNA digested with MspI restriction enzyme and then extracted with ZR-DNA Clean & Concentrator™-5 kit (Zymo Research, Cat# D4014). According to Illumina’s specified guidelines ...
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bioRxiv - Microbiology 2023Quote: ... a 4.5 ml sample of each microcosm and extracted bacterial DNA with a Quick-DNA Fecal/Soil Microbe Miniprep Kits (Zymo Research, Irvine, California, USA). Two negative controls that contained the SH salt medium alone were included in the process of DNA extraction ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mixture was incubated at 65 °C for 3 h and the RNA was then isolated by using the Zymo RNA clean and concentrator-5 kit (Zymo Research, cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were run on 1% agarose gel and purified using the Zymoclean gel DNA recovery kit (Zymo Research).
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bioRxiv - Microbiology 2022Quote: ... RNA extractions were performed from 1 ml LB culture using the Direct-zol RNA MiniPrep Plus (Zymo Research, ZR2073). Total RNAs were reverse-transcribed into cDNAs using iScript™ cDNA Synthesis Kit (BioRad ...
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bioRxiv - Systems Biology 2022Quote: ... The flow through was mixed with 1 volume of ethanol then transferred to a Zymo Spin IIC column (Zymo) and centrifuged ...
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bioRxiv - Biochemistry 2020Quote: ... The entire PCR reaction is run on 1% agarose gel and the DNA at 230bp was purified (Zymo Research). See Supplemental Figure S2.
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL ...
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bioRxiv - Plant Biology 2023Quote: RNA was purified from seedlings Col-0 and cpk32-1 using the Direct-zol RNA MiniPrep Kit (Zymo Research). The reverse-transcriptase cDNA synthesis reaction was carried out by the RevertAid Reverse Transcriptase (ThermoFisher).
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bioRxiv - Genomics 2023Quote: ... One of each type of swabs was placed into 1 mL DNA/RNA Shield (Zymo Research, Irvine California, USA) and the other of each into 1 mL methanol ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae eby100 was retransformed using 1 µg of pGal1 – Aga2p_eGFP following the protocol of the EZ yeast transformation kit II (Zymo). 100 µL of cells were selected on SDCAA plates ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant and pellet samples on non-denaturing 1% agarose gel we extracted RNA with TRI reagent® (ZYMO Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant and pellet samples on non-denaturing 1% agarose gel we extracted RNA with TRI reagent® (ZYMO Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Bacterial RNA was extracted from ∼1 × 108 CFU after AMP exposure (above) using Direct-zol RNA Miniprep kit (Zymo) as per manufacturer’s instructions ...