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Citations for Zymo Research :
1101 - 1150 of 1348 citations for 7 Boc 3 chloro 5 6 7 8 tetrahydroimidazo 1 2 a pyrazine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Total RNA was purified from the aqueous phase using the RNA Clean & Concentrator-5 kit with DNase I treatment to remove genomic DNA contamination (Zymo Research R1013). RNA quantity was determined with the Qubit RNA Broad Range Assay (ThermoFisher Scientific Q10210) ...
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bioRxiv - Neuroscience 2023Quote: ... The sequencing libraries were then run on a 10% TBE polyacrylamide gel and purified using the DNA Clean & Concentrator-5 kit (Zymo Research D4013).
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bioRxiv - Cancer Biology 2023Quote: ... The rRNA-depleted and DNAse-treated RNA was then cleaned with the Zymo RNA Clean and Concentrate-5 kit (Zymo Technologies, USA) and eluted in 8 uL nuclease-free water.
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bioRxiv - Biochemistry 2023Quote: ... The tagmented DNA was cleaned up using Zymo DNA Clean and Concentrator-5 columns with 5X binding buffer (Zymo, cat. no. D4004). 5 μL of purified DNA out of 12.5 μL purified product was amplified in a 25 μL PCR reaction containing NEBNext PCR master mix (1X final) ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were purified from agarose gels using the Zymoclean® Gel DNA Recovery Kit or from PCR reactions using the DNA Clean & Concentrator®-5 DNA kit from Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted by phenol-chloroform for the ZNF469 profiling to keep the small DNA fragments and with the DNA Clean & Concentrator-5 kit (Zymo Research, D4004) for the H3K27ac profiling ...
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bioRxiv - Developmental Biology 2020Quote: ... Extracted DNA was treated with RNAse A (1 μL of 1 mg/mL added to samples of 100 μL for 30 minutes at room temperature) and then purified and concentrated using a DNA Clean and Concentrator-5 Kit (Zymo Research Corp., USA). The concentration of genomic DNA was measured on a QUBIT 4 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: Genomic DNA samples were also used for global DNA methylation analysis using the 5-mC DNA ELISA Kit (Zymo Research, Irvine, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from the sorted cells as described (Hrvatin et al., 2014a) and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1013). Complementary DNA (cDNA ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed from the 0.22 μm filters (containing the 5–0.22 μm microbial size fraction) using the ZR Soil Microbe DNA MiniPrep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: IRES-Venus and human SNCA fragments were amplified by PCR (MJ Research, PTC-200 Peltier Thermal Cycler) and purified using DNA Clean & Concentrator™-5 Kit (Zymo Research, D4003) to provide 20 μl of eluted DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was isolated using phenol-chloroform extraction and then purified and concentrated using ethanol precipitation and washing on a silica column (RNA Clean & Concentrator-5, Zymo Research, Irvine, CA). Total RNA samples were reverse transcribed (ThermoFisher SuperScirpt IV Vilo) ...
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bioRxiv - Microbiology 2023Quote: ... Remaining material from the same DNA extract of subculture 19.9 was then concentrated using the DNA Clean and Concentrator-5 kit (Zymo Research; Irvine, California, U.S.A.) and used for long read sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was removed from the purified RNA samples using RNA Clean & Concentrator™-5 columns with on-column DNase digest (Zymo Research, R1014). For rRNA-depleted samples ...
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bioRxiv - Systems Biology 2024Quote: ... Microbial genomic DNA was extracted from frozen feces (∼100 mg, or 5 pellets) with the ZymoBIOMICS 96 MagBead DNA Kit with lysis tubes (Zymo Research, Irvine, CA), according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was purified and the DNA oligos were removed from samples using RNA Clean & Concentrator™-5 columns with on-column DNase digest (Zymo Research, R1014) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or TRI Reagent (Zymo, #R2050-1) for gene expression analysis ...
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bioRxiv - Genomics 2021Quote: ... digestion with 1% zymolyase (Zymo Research) to weaken ascus walls ...
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bioRxiv - Plant Biology 2020Quote: ... a qRT-PCR product was purified from a complete PCR reaction using the Zymo DNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA) and eluted in 10 μl of nuclease-free water ...
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bioRxiv - Neuroscience 2019Quote: ... Beads were then washed several times with buffers and RNA purification was performed on eluted beads (Zymo RNA Clean & Concentrator −5, R1013, Zymo Research, Irvine, CA, USA). RNA quality and concentration were determined via Agilent BioAnalyzer prior to sequencing.
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bioRxiv - Molecular Biology 2019Quote: ... Briefly dissected mosquito midguts were collected in pools of 5 and viral RNA was isolated using the Zymo Quick-RNA™ Viral Kit (Zymo, Irvine, CA, USA). Viral RNA samples were reverse-transcribed (RT ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA extractions were performed upon 5 million trophozoites of Nf69 using Quick-DNA Miniprep Plus Kit according to manufacturer recommendations (Zymo Research, Irvine, CA, USA). In short ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The sample was then centrifuged at 2300 rpm for 5 min and the supernatant was collected and concentrated and purified using a Zymo-Spin V reservoir (Zymo Research Irvine, CA, USA) and Qiagen MinElute spin column (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... a 4.5 ml sample of each microcosm and extracted bacterial DNA with a Quick-DNA Fecal/Soil Microbe Miniprep Kits (Zymo Research, Irvine, California, USA). Two negative controls that contained the SH salt medium alone were included in the process of DNA extraction ...
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bioRxiv - Neuroscience 2023Quote: RRBS libraries were prepared from 200 ng of genomic DNA digested with MspI restriction enzyme and then extracted with ZR-DNA Clean & Concentrator™-5 kit (Zymo Research, Cat# D4014). According to Illumina’s specified guidelines ...
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bioRxiv - Microbiology 2019Quote: ... The Ribo-Zero rRNA Removal Kit (Bacteria) (illumina®, San Diego, CA) was used for mRNA enrichment and the RNA Clean & Concentrator™ −5 (Zymo Research, Irvine, CA) for final purification ...
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bioRxiv - Biophysics 2019Quote: ... RNA samples were purified after each of the steps above (dephosphorylation, phosphorylation and ligation) with the RNA Clean & Concentrator™-5 (Zymo Research Europe, Freiburg, Germany) and eluted with nuclease-free water ...
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bioRxiv - Microbiology 2020Quote: ... The final pool volume was reduced to concentrate the sequencing library using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). The pooled library concentration was determined using the Quant-iT™ High-Sensitivity DNA Assay Kit (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from mixtures of KU-1 or BL3.1 and BTL26 for a total of 1 × 106 cells using Quick-DNA Miniprep Kits (Zymo Research). The DNA concentrations of the samples were measured by UV absorbance at 260 nm using a NanoDrop 8000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... all mouse tissues obtained from BSL3 were treated with 1% Triton by volume or placed in TRIzol (Zymo R2050-1) to inactivate virus.
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bioRxiv - Immunology 2024Quote: ... the supernatant (200uL) from one well from each dilution series was mixed 1:1 with 2x RNA/DNA Shield (Zymo) for viral inactivation and RNA extraction as described above ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 mg/ml Proteinase K (Zymo Research) for 1 hour at 55°C ...
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bioRxiv - Microbiology 2021Quote: ... A 10:1 RNA/DNA shield from Zymo was added to each saliva sample to maintain samples during shipping.
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bioRxiv - Microbiology 2022Quote: ... and 100μL TRIzol (Zymo Research, R2050-1-50) was added to each well followed by isolation of total RNA using Direct-Zol RNA Mini-Prep Plus Kit (Zymo Research ...
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bioRxiv - Neuroscience 2024Quote: ... or preserved in TRI Reagent (Zymo, #R2050-1) for gene expression analysis.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with RNase for 1 hour and proteinase K for 1 hour before ChIP DNA Clean & Concentrator (Zymo Research) to extract the DNA.
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bioRxiv - Microbiology 2024Quote: ... The upper aqueous phase was transferred to an RNase-free tube and mixed in a 1:1 ratio with 100% EtOH and processed via the clean and concentrator kit (Zymo Research). RNA was eluted in 15 µL RNase-free ddH2O ...
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bioRxiv - Microbiology 2024Quote: ... 250 µl aqueous phase was mixed 1:1 with 75% ethanol and transferred into the Zymo-Spin IC Column from the RNA Clean & Concentrator Kit (Zymo Research). RNA clean-up and collection was done following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... The RNA was mixed with ethanol absolute at a 1:1 v/v ratio and submitted to a spin column-based purification protocol (Direct-zol RNA Miniprep kit, Zymo Research), including a DNAse treatment step ...
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bioRxiv - Physiology 2024Quote: ... the column was brought to room temperature for 5 min and the RNA was spun out into fresh tubes (50 μL for Zymo-II and 60 μL for Zymo-IICG columns). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 x ZymoTaq PreMix (Zymo Research, Irvine CA, USA) and 10μM of each primer ...
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bioRxiv - Genomics 2020Quote: ... with Oligo binding buffer (Zymo Research, D4060-1-10). The final product was assumed to have full yield (for a list of sequences ...
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bioRxiv - Immunology 2024Quote: ... Anti-DNA-HRP (1:100; Zymo Research; Irvine, CA), was added to the plate and incubated for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... then homogenized with TRI Reagent (Zymo Research, R2050-1) by the gentleMACS Dissociator (Miltenyi Biotec 130-093-235) ...
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bioRxiv - Microbiology 2020Quote: ... 75μl supernatants were collected at 48hpi and inactivated 1:1 in 1X DNA/RNA Shield for RNA extraction and RT-qPCR analysis (Zymo Research, Irvine, CA). RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: Viral load was quantified from pharyngeal and nasal swabs taken from infected animals at TNPRC as previously described (1) Swab and bronchial brush samples were collected in 200 μL of DNA/RNA Shield 1× (catalog number R1200; Zymo Research, Irvine, CA) and extracted for viral RNA using the Quick-RNA viral kit (catalog number R1034/5 ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected at 48hpi and inactivated 1:1 in 1X DNA/RNA Shield for RNA extraction and RT-qPCR analysis (Zymo Research, Irvine, CA). RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... by adding RNA recovery buffer (R1070-1-10; Zymo Research) to the excised gel slices and further incubating at 70°C (1,500 rpm ...
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bioRxiv - Genomics 2019Quote: ... by adding RNA recovery buffer (Zymo Research, R1070-1-10) to the excised gel slices and further incubating at 70°C (1500 rpm ...
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bioRxiv - Evolutionary Biology 2023Quote: Cultures were then resuspended in a 1% zymolyase (Zymo Research) solution to weaken ascus walls ...