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Citations for Zymo Research :
1101 - 1150 of 1448 citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Total RNA was extracted from SCD or HD HSPCs (15, 24, 48, 72 hours and 6 days post-transfection) using the Quick-DNA/RNA Miniprep (ZYMO Research). RNA was treated with DNase using the DNase I kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from Calu-3 lysates using Direct-zol RNA Miniprep Plus Kit (Zymo Research) and reverse transcribed using High-Capacity cDNA RT kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA from AsPC1 or OV90 cells transfected with 100 nM siSMNDC1 or siCON for 48 h was extracted using Quick-RNA MiniPrep (R1055, Zymo Research, Irvine, CA). Three biological repeats were performed ...
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bioRxiv - Synthetic Biology 2020Quote: ... were pretreated with a solution of β-mercaptoethanol plus 60 mM EDTA and then incubated with 6 U/mL zymolyase (Zymo Research) in Buffer I to remove the cell wall ...
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bioRxiv - Microbiology 2022Quote: ... 6-10 aphids colonized with fluorescent bacteria from each condition were collected and crushed in DNA/RNA shield (Zymo Research, CA, USA). Whole aphid RNA was purified using an RNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Cancer Biology 2023Quote: In vitro-infected keratinocytes and SCC cells were lysed in 6-wells with 1mL TRIzol Reagent and the RNA was purified by using the Direct-zol™ RNA Miniprep kit (Zymo Research). The procedure was performed according to the manufacturer protocol except for an additional 1 min centrifugation after the last washing step to completely remove eventual ethanol leftover ...
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bioRxiv - Plant Biology 2023Quote: ... Each biological replicated was generated by pooling three individual ChIP pull-downs (each consisting of 6 g of whole seedling tissues) during DNA purification using the ChIP DNA Clean & Concentrator Kit (Zymo Research, D5201). The samples were sent to BGI Genomics for library preparation and sequencing using DNBSEQ ...
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bioRxiv - Genomics 2024Quote: ... The pre-adenylated linker was eluted in 6 µl H2O following the clean-up using the Oligo Clean & Concentrator Kit (Zymo Research, D4060).
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bioRxiv - Synthetic Biology 2022Quote: ... 10 µL of Mag-Binding beads (Zymo Research, #D4100-2-24) was added and the sample was incubated for 3 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from 3 x 105 Raji cells using Direct-zolTM RNA MiniPrep kit (Zymo Research) according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The band at roughly 3 kb was extracted using a Zymoclean Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-500 ng of DNA from each sample was bisulfite-converted using the Zymo EZ DNA Methylation Kit (Zymo Research, Irvine, CA USA) following the manufacturer’s protocol and the specified modifications for the Illumina Infinium methylation assays ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The liver of control and BPA lineage fish (9 females and 6 males) were used for total RNA extraction by using Quick RNA/DNA Miniprep Plus Kit (#D-7003, Zymo Research, CA, USA) according to the manufacturer’s protocol as previously described60 ...
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bioRxiv - Bioengineering 2021Quote: Genomic DNA was extracted from 3×106 HEK293_LPCD19/BiTE cells using Quick-DNA midiprep kit (#D4075, Zymo Research). Gene specific primers annealing to the signal sequence (5’-CTGGTCCTGCATCATCCTGTTTC-3’ ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... We then lysed 3 dpf larvae individually by rigorously pipetting in 55 μl DNA/RNA Shield (Zymo Research) at RT followed by overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and were filled with 3 µl of the RNA Lysis Buffer (Quick-RNA Microprep Kit, Zymo, Irvine, Ca). The pipette was mounted on a micromanipulator and used to aspirate 2– 10 corpuscles from duck bill skin by applying negative pressure using a high-speed pressure clamp system (HSPC-1 ...
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bioRxiv - Plant Biology 2024Quote: Genomic DNA was extracted from 3-week-old seedlings with Quick-DNA Plant/Seed Miniprep Kit (ZYMO, D6020). 200ng fragmented genomic DNA (200bp-500bp ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL Ribolock) and recovered by RNA clean and concentrator kit (Zymo) using the manufactureŕs protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 60 per biological replicate) were dissected from 6-day-old females in cold 1X PBS and stored in RNA/DNA shield (Zymo Research Europe; Freiburg, Germany) at −80°C until RNA extraction ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA products were separated on a 3% agarose gel and then extracted using gel extraction kit (ZYMO research). In vitro transcription with T7 RNA polymerase and in vitro Dicer cleavage assays were performed as previously described (Sheng et al ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from 3×105 cells per replicate using Direct-Zol RNA Miniprep Kit (Zymo Research, USA) according to the manufacturer’s protocol and shipped in dry ice to the MDC/BIH Genomic Core Facility for quality assessment using TapeStation (Agilent Technologies) ...
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bioRxiv - Systems Biology 2023Quote: ... 3 μl of reaction mix were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Cell Biology 2024Quote: ... The column was washed twice with 2 ml DNA Wash Buffer (Zymo Research) by a second step of centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 ml Trizol (R2050-1, Zymo Research) as well as 200 µl chloroform were added to the interface and phase separation and removal were repeated ...
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bioRxiv - Genomics 2020Quote: ... the total RNA was extracted from active and tun samples (3 replicates) using Direct-Zol RNA MicroPrep Kits (Zymo Research), and the sequencing library was constructed using SMARTer v4 Ultra Low Input RNA Kit for Sequencing (Clonetech ...
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bioRxiv - Genetics 2022Quote: Yeast colonies containing bait and prey constructs that grew on Trp−/Leu−/His− + 25 mM 3-Amino-1,2,4-triazole (3AT) selection plates were extracted using the Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004) followed by Sanger sequencing for gene identification ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from 3 biological replicates of 100mg of frozen tissue of each accession using the Quick-DNA/RNA Miniprep kit (Zymo) following manufacturer’s instructions with the following modifications ...
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bioRxiv - Genomics 2023Quote: ... 3 colonies were randomly selected and further expanded for plasmid DNA purification (Zyppy™ Plasmid Purification Kit; Zymo research #D4019). Extracted DNA was then sequenced to verify the integrity of the cloned fragments.
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from a single leaf of 3-week-old T4 Setaria lines using the Directzol RNA kit (Zymo) including on-column DNAse treatment ...
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bioRxiv - Genomics 2021Quote: Cells were FACS sorted into 384-plate wells containing 2 µl lysis buffer (Zymo M-digestion buffer also containing proteinase K) ...
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bioRxiv - Genomics 2020Quote: ... run on 2 % agarose gel and extracted using the Zymo Gel Extraction Kit (Zymo) with final elution in 16 uL ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2×106 cells were stored in 1X DNA/RNA Shield (Zymo Research, U.S.) at -80°C for downstream molecular analysis.
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bioRxiv - Plant Biology 2023Quote: ... 2 ml of cultures were harvested and Direct-Zol RNA MiniPrep kit (Zymo Research) was used ...
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bioRxiv - Physiology 2024Quote: ... 2 x isopropanol and purified with Zymo RNA clean and concentrator kit (Zymo Research) for subsequent RNA purification.
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bioRxiv - Cancer Biology 2022Quote: ... Individual 50 μL reactions were run on a 3% agarose gel and libraries were purified using the Zymoclean Gel DNA Recovery kit (D4007, Zymo Research). Sequencing was performed using standard Illumina instructions ...
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bioRxiv - Microbiology 2022Quote: ... and stored at -20°C until (≤ 3 days) the DNA was extracted via the Zymo Quick-DNA Fecal/Soil Microbe kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2021Quote: ... The nascent RNA was purified by adding 3 volumes of TRIzol and processed with the Direct-zol RNA Miniprep kit (Zymo Research) with addition of 1/100 volume of 100 mM DTT to each supplied wash buffer.
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bioRxiv - Microbiology 2021Quote: ... and genomic DNA was extracted from 3 mL of overnight (TSB) broth culture using the ZR Duet DNA/RNA MiniPrep Plus kit (Zymo Research) following manufacturer’s protocol for the “suspended cells” option ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Microbiology 2022Quote: ... The high molecular weight bands were excised with a razor and dissolved in 3 volumes of Zymo Agarose Dissolving Buffer (Zymo D4001) at 50°C ...
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bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from 2 × 106 cells using the Quick-RNA miniprep kit (Zymo) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: RNA was extracted from 2 dpf whole embryos using the Directzol RNA mini kit (Zymo). cDNA was synthesised and amplified using Thermo Fisher SuperScript II Reverse Transcriptase Kit ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (300µl) was mixed with 30µl of Proteinase K (Zymo Cat #D3001-2-20) digestion buffer and 15µl of Proteinase K in a new tube using a brief vortex step ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2-infected and Mock-infected samples were treated with RNA Shield (Zymo Research) to preserve their genetic integrity and inactivate the virus ...
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bioRxiv - Genomics 2022Quote: ... then thawed and combined with 2 volumes of DNA Shield (Zymo Research, Irvine, CA, USA). Genomic DNA was extracted using a Quick DNA Miniprep Plus kit (Zymo Research ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each swab was deposited in a separate 2 mL DNA/RNA shield tube (Zymo; #R1102) immediately after collection ...
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bioRxiv - Molecular Biology 2024Quote: ... DNase I (1 U ml−1, Zymo Research, #E1010), MgCl2 (5 mM ...
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bioRxiv - Genomics 2022Quote: ... into the 20 μl ligation reaction for incubation with 30 minutes at 30°C and 3 minutes at 65°C to inactivate the enzymes followed by column purification (Zymo Research, R1016). Before the reverse transcription (RT) ...
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bioRxiv - Plant Biology 2020Quote: Genomic DNA was extracted from aerial parts of 3-week-old seedlings using Quick-DNA Plant/Seed Miniprep Kit (Zymo research, D6020) according to the manufacturer’s protocol ...