Labshake search
Citations for Zymo Research :
1051 - 1100 of 1397 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... those in the correct size range were extracted and purified using a Zymoclean gel DNA recovery kit (Zymo Research, Irvine, CA, USA) and Sanger sequenced (Quintara Biosciences ...
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bioRxiv - Genomics 2020Quote: DNA was extracted using 4-10 unstained slides of FFPE tissue using the Pinpoint Slide DNA Isolation system (Zymo Research, Orange, CA). In brief ...
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bioRxiv - Plant Biology 2020Quote: ... The yeast expression vector constructs or empty vector were transformed into yeast Saccharomyces cerevisiae WAT11 (Pompon et al., 1996; Truan et al., 1993) by using Yeast Transformation II Kit (ZYMO, CA, USA) and screened on synthetic SD-dropout medium lacking uracil (SD-Ura ...
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bioRxiv - Genetics 2021Quote: ... All samples were randomized into 96-well plates for RNA extraction with the Direct-zol-96 RNA Kit (Zymo Research, Irvine, CA). Libraries constructed with the Lexogen QuantSeq 3′ mRNA-Seq Library Kit FWD (Lexogen ...
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bioRxiv - Genetics 2020Quote: 200ng of liver genomic DNA per sample was treated with sodium bisulfite using the EZ DNA Methylation kit (Zymo Research, Irvine, CA). Standards using mouse genomic DNA of known percentage methylation (0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA was extracted from the pellets and the highly infected samples used the Zymo Quick-RNA kit (Zymo Research LLC, Irvine, CA). Three samples with unfiltered hemolymph ...
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bioRxiv - Plant Biology 2020Quote: ... As a positive control we included a mock microbial community in three wells (ZymoBiomics Microbial Community Standard, Zymo Research, Irvine, CA, USA). We used the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Some DNA extracts that showed high levels of degradation were size-selected using Select-a-Size column or bead kits (ZYMO, Irvine, CA). High molecular weight ...
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bioRxiv - Biochemistry 2020Quote: ... The Carbon-Clarke equation was used to calculate how many colonies were needed to ensure a 99% confidence that all guides in the library was represented after each transformation (∼37,000 colonies).20 Colonies were scraped and midi-prepped (Zyppy Plasmid Midiprep Kit, Zymo Research, Irvine, CA) in preparation to be transformed into strain W.
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bioRxiv - Biochemistry 2021Quote: ... at 10 m/s for 90 s to lyse cells and then using the Zymo Research Direct-Zol RNA MiniPrep Plus kit (Zymo, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene using primers custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
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bioRxiv - Genetics 2021Quote: ... The adaptor-ligated DNA samples were subjected to bisulfite conversion by the EZ DNA Methylation-Gold kit (Zymo Research, Irvine, CA, USA) and amplified by PCR with the KAPA HiFi HotStart Uracil+ ReadyMix kit (Roche ...
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bioRxiv - Microbiology 2021Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Bacterial 16S primers amplify the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Microbiology 2022Quote: ... Fungal DNA from the cultures was extracted using a ZymoBIOMICS® Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2022Quote: ... followed by selective recovery of total RNA using an RNA spin column clean-up kit (RNA Clean & Concentrator-5; Zymo, Irvine, CA). RNA was extracted from cultured cells by phase separation with chloroform followed by RNA precipitation in isopropanol as previously described [38] ...
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bioRxiv - Genomics 2022Quote: ... 500ng of high-quality genomic DNA was bisulfite converted using the Zymo EZ-96 DNA Methylation kit (Zymo Research, Irine, CA, USA). Bisulfite converted samples were then amplified ...
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bioRxiv - Microbiology 2022Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 300μl of the resulting coral slurry using ZymoBIOMICS™ DNA/RNA Miniprep (Zymo Research Corp., Irvine, CA, USA) following an additional 2-step enzyme incubation to increase recovery of bacterial DNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
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bioRxiv - Developmental Biology 2022Quote: RNA was extracted from flash-frozen fetal liver (left lobe) samples using a Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA)) per manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and from cell lines using and Direct-zol™ RNA MiniPrep w/ Zymo-Spin™ IIC Columns (Zymo Research, Irvine, CA, USA). ...
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bioRxiv - Microbiology 2022Quote: ... we added negative DNA extractions and PCR controls from each time point and mock communities (ZymoBIOMICS microbial community standard, Zymo, Irvine, CA) to each library for additional quality control and inference.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted and genomic DNA was removed using the Direct-zol 96-well MagBead RNA Kit (Zymo Research, Orange, CA). cDNA was synthesized from total RNA using the iScript™ Reverse Transcription System (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... All samples were equilibrated to a total volume of 50 μL and then DNA was extracted using Zymo Quick-DNA Microprep Kit (Zymo Research, CA). Procedure was performed using modifications to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... and 500ng of DNA from each sample was bisulfite converted using the Zymo EZ DNA Methylation Kit (Zymo Research, Irvine, CA USA) following the manufacturer’s protocol using the specified modifications for the Illumina Infinium Methylation Assay ...
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bioRxiv - Microbiology 2021Quote: ... The fungal isolates were identified by extracting genomic DNA with the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research; Irvine (Fig, CA), amplifying and sequencing the internal transcribed spacer (ITS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: DNA was extracted from 100 mg caecum content using the Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA, USA) with minor adaptations from manufacturer’s instructions (Ducarmon et al ...
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bioRxiv - Physiology 2021Quote: ... Total RNA was extracted from each supernatant with the Zymo Quick-DNA/RNA™ Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Genomic DNA (1 μg) was treated with sodium bisulfite using the EZ DNA Methylation Gold Kit per manufacturer’s instructions (Zymo Research; Irvina, CA). Bisulfite converted DNA (∼20 ng ...
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A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite AdipogensbioRxiv - Pharmacology and Toxicology 2021Quote: ... Total RNA was extracted and genomic DNA was removed using the Direct-zol MagBead RNA Kit and following manufacturer’s protocol (Zymo Research, Orange, CA). RNA concentrations and contamination were determined spectrophotometrically using a Nanodrop (ND-1000 ...
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bioRxiv - Microbiology 2021Quote: Six PCR reactions from each sample were pooled and purified with the DNA Clean & Concentrator™ kit (Zymo Research, Irvine, CA, USA), and quantified with Qubit 1.0 fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was purified and treated with DNase by using the Direct-zol RNA MiniPrep kit (Zymo Research Corporation, Orange, CA, USA), according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 20 mg of each tissue using the ZYMO Quick-DNA™ Miniprep Plus Kit (Zymo Research, Irvine, CA). Resulting DNA was amplified neat and diluted 1:2 ...
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bioRxiv - Immunology 2021Quote: DNA for HLA typing was isolated from PBMCs using the Quick-DNA/RNA™ Microprep Plus Kit (Zymo Research, Irvine, CA, USA). High throughput-HLA typing was performed by the Institute of Transplantation Diagnostics and Cell Therapeutics (ITZ) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... methylated indexed adapters were ligated and 20 µL of cleaned DNA fragments were subjected to sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research, CA). Pre-amplification of the bisulfite-converted library was carried out for an equimolar pool of eight samples and was then subjected to exome capture at 47°C for 45 hours ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were isolated from agarose gel after electrophoresis and purified using Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, CA, USA). Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was extracted from overnight cultures grown in 5 mL BHI broth using the Zymo BIOmics DNA Miniprep Kit (Zymo, Irvine, CA).
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bioRxiv - Genomics 2023Quote: ... bisulfite sequencing libraries were constructed according to the manufacturer’s instructions using the EZ DNA Methylation-GoldTM Kit (Zymo Research, Irvine, CA, USA). Bisulfite sequencing libraries were sequenced to generate 150 bp paired-end reads ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was then extracted from each sample using the Quick-RNA MiniPrep Kit according to the manufacturer’s protocol (Zymo Research, Irvine, CA). The total amount of RNA in each sample was subsequently quantified with a Qubit 2.0 fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Cross-links between the DNA fragments and histones were reversed and the DNA fragments were recovered using the ChIP DNA Clean & Concentrator Kit (Zymo, Irvine, CA). Two biological replicates were performed for each group ...
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bioRxiv - Systems Biology 2022Quote: ... 100 μl of the diluted culture was spread on LB agar plates supplemented with 25 μg/ml of 2,3,5-triphenyltetrazolium chloride to stain colonies bright red using Rattler Plating Beads (Zymo Research, Irvine, CA, USA), and incubated for 12 to 24 hours ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from snap-frozen cell pellets using the Quick-RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA from the heat lysed ZeptoMetrix MTB verification panel samples was purified and eluted in 40μL TE using Oligo Clean & Concentrator (Cat# D4061, Zymo Research, CA, USA). 5μL of extracted DNA was used as input for the LAMP assay.
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bioRxiv - Microbiology 2024Quote: ... The amplified products were purified using the QIAquick PCR purification kit and underwent size selection using 1.5% low-melt agarose gels and Gel DNA Recovery Kit (Cat. No. D4002, Zymo Research, Irvine, CA). A final pool was made after quantification of each sample using the Qubit high-sensitivity dsDNA kit (Cat ...
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bioRxiv - Genetics 2023Quote: Genomic DNA (gDNA) was isolated from two G1 adults per mutant line using Zymo Quick-DNA MicroPrep kit (Zymo Research, Irvine, CA), and the yield was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Plant Biology 2024Quote: Total RNA from seedlings was isolated using a Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research, Irvine, CA). cDNA was synthesized using Oligo(dT ...
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bioRxiv - Genomics 2023Quote: ... extracted RNA was concentrated and treated with DNAse I utilizing on-column digestion following the manufacturer’s protocol (Zymo Research, Irvine, CA, USA). The resulting RNA was depleted of ribosomal RNA for two hours ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the flash-frozen cell pellets using the Zymo dual DNA/RNA extraction kit (D7001, Zymo, Irvine, CA, USA). Extractions were performed in batches of 12 where all treatments and timepoints per individual were extracted in the same batch ...
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bioRxiv - Microbiology 2023Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...