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Citations for Zymo Research :
1051 - 1100 of 1165 citations for 3 5 Diacetoxy 2 acetoxymethyl 6 phenethyl tetrahydro pyran 4 yl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: DNA from 32 skull base chordomas and 4 NP samples were bisulfite converted with EZ-96 DNA Methylation kit (Zymo Research, Orange, CA, USA) and used for genome-wide DNA methylation profiling with Methylation EPIC (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated using the Illumina NextSeq 500 High Output Kit v2.5 (Cat. #20024908) and eluted in DNA Elution Buffer (Zymo Research, Cat. #D3004-4-10). Library concentration and average size was verified using the TapeStation DNA 5000 Assay Reagents and Screen Tapes (Agilent ...
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bioRxiv - Plant Biology 2020Quote: ... the 50 µL aliquot plus 5 µL of 10% (w/v) SDS was purified using the Zymo RNA clean and concentrator kit (Zymo Research; R1017). The total RNA was rRNA-depleted with the Ribo-Zero plant leaf kit (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... templates were run on a 1% agarose gel to confirm cutting and the reactions were purified with Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). 1ug of linearized template was used in a 100uL T7 RNA Polymerase (purified in house ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were run on a 1% agarose gel to check the product size and purified using Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). To create the cloning insert ...
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bioRxiv - Synthetic Biology 2021Quote: Plasmids were isolated from 5-10 mL yeast cultures either by alkaline lysis (39) or using Zymo Yeast Miniprep Kit I (Zymo Research D2001). Plasmids were then recovered into DH10B ElectroMax E ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Cell Biology 2022Quote: ... followed by selective recovery of total RNA using an RNA spin column clean-up kit (RNA Clean & Concentrator-5; Zymo, Irvine, CA). RNA was extracted from cultured cells by phase separation with chloroform followed by RNA precipitation in isopropanol as previously described [38] ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested codon random mRFP with singlestranded overhangs was cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). The complete 15 μl containing the codon random mRFP library was used in a ligation reaction to generate the plasmid library.
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bioRxiv - Molecular Biology 2022Quote: ... The ligation mixture was cleaned and concentrated to 10 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). 1 μl of the codon randomised mRFP library was transformed into electrocompetent DH10B cells (20 μl competent cells ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions containing the dsDNA block were cleaned and concentrated to 20 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 4 μl Gel Loading Dye ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using TRIzol-based (Fisher: 15596026) phenol-chloroform extraction and purified with RNA Clean and Concentrator-5 spin columns (Zymo research: R2050). RNA concentration and quality was assessed with a NanoDrop 1000 spectrophotometer (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were extracted from a 10 mL culture (5 OD) of sorted cells subpopulations using the Zymoprep™ Yeast Plasmid Miniprep II (Zymo Research) following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Biophysics 2021Quote: ... RNA was then extracted from the aqueous phase using a spin column-based purification kit (RNA Clean & Concentrator-5, Zymo Research # R1015) and reverse-transcribed into cDNA using SuperScript VILO Master Mix (Thermo Fisher # 11755050) ...
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmentation was performed for 30 minutes at 37°Con a rotator and DNA was purified using the DNA Clean and Concentrator 5 kit (Zymo Research D4004). PCR was performed as previously described [23] using 11 total cycles ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (500 x g for 5 minutes) and lysed for RNA and DNA extraction using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research, D7003). RNA and DNA were eluted in water ...
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bioRxiv - Cell Biology 2024Quote: ... and all reactions with the appropriate molecular weight product were combined and cleaned using the DNA Clean and Concentrator 5 kit (Zymo Research D4013). 10 µg LentiCRISPRv2-opti (Addgene #163126 ...
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bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s instructions and 35 µL of the supernatant were treated with the RNA Clean & Concentrator Kit 5 (Zymo Research, Irvine, USA) using an adapted protocol (the RNA prep buffer step was performed twice ...
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bioRxiv - Biochemistry 2023Quote: ... The tagmented DNA was cleaned up using Zymo DNA Clean and Concentrator-5 columns with 5X binding buffer (Zymo, cat. no. D4004). 5 μL of purified DNA out of 12.5 μL purified product was amplified in a 25 μL PCR reaction containing NEBNext PCR master mix (1X final) ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was extracted from overnight cultures grown in 5 mL BHI broth using the Zymo BIOmics DNA Miniprep Kit (Zymo, Irvine, CA).
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bioRxiv - Genetics 2023Quote: ... Total RNA was purified from the aqueous phase using the RNA Clean & Concentrator-5 kit with DNase I treatment to remove genomic DNA contamination (Zymo Research R1013). RNA quantity was determined with the Qubit RNA Broad Range Assay (ThermoFisher Scientific Q10210) ...
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bioRxiv - Neuroscience 2023Quote: ... The sequencing libraries were then run on a 10% TBE polyacrylamide gel and purified using the DNA Clean & Concentrator-5 kit (Zymo Research D4013).
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bioRxiv - Cancer Biology 2023Quote: ... The rRNA-depleted and DNAse-treated RNA was then cleaned with the Zymo RNA Clean and Concentrate-5 kit (Zymo Technologies, USA) and eluted in 8 uL nuclease-free water.
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were purified from agarose gels using the Zymoclean® Gel DNA Recovery Kit or from PCR reactions using the DNA Clean & Concentrator®-5 DNA kit from Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted by phenol-chloroform for the ZNF469 profiling to keep the small DNA fragments and with the DNA Clean & Concentrator-5 kit (Zymo Research, D4004) for the H3K27ac profiling ...
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bioRxiv - Microbiology 2024Quote: ... 75 μl of nuclease-free water was added to the mix followed by 270 μl of RNA binding buffer (RNA Clean and Concentrator-5 column kit, Zymo Research, R1015) and 400 μl of 100% ethanol ...
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bioRxiv - Microbiology 2024Quote: ... was amplified using flanking primers and the resulting PCR products were purified using a DNA Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA). STm 14028s carrying the pKD46 plasmid (64 ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were treated with 0.5 mg/ml proteinase K at 65 ℃ for one hour and purified with Zymo ChIP DNA Clean Concentrator kit (Zymo Research D5205) as the manufacturer’s manual and quantified by QUBIT ...
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bioRxiv - Molecular Biology 2024Quote: ... The aqueous phase was retained for RNA purification using the Zymo RNA Clean & Concentrator-5 kit following manufacturer directions for the capture of small RNA fragments with slight modification (Zymo Research, R1013). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... This library pool was then size selected on a 2% agarose gel by cutting between 150-800 nucleotides and performing gel purification (Zymo). To determine the loading density of the final pooled library ...
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bioRxiv - Microbiology 2020Quote: The Zymobiomics microbial community DNA standard consisting of 8 bacterial and 2 fungal genomic DNAs (cat. no. D6305) was obtained from Zymo Research (Irvine ...
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bioRxiv - Genomics 2022Quote: Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI, USA], 2 U/mL zymolyase [Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... Filters were then transferred into a 1.5 ml tube and the filter-associated cells were resuspended by adding 1 ml 1X PBS + 0.05% Triton X-100 detergent and agitating the tubes at high speed for 2 minutes using a Genie Disruptor (Zymo). This suspension as well as the inoculum were diluted ...
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bioRxiv - Genomics 2021Quote: ... RNA was extracted from inactivated swab samples using the Quick DNA/RNA Viral Magbead kit from step 2 of the DNA/RNA purification protocol (Zymo) (files.zymoresearch.com/protocols/_r2140_r2141_quick-dna-rna_viral_magbead.pdf).
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bioRxiv - Developmental Biology 2023Quote: DNA was extracted from fin clips stored in 100% ethanol for <2 weeks using the PCR biosystems rapid extract lysis kit or Quick-DNATM Miniprep Plus kit by Zymo research ...
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bioRxiv - Microbiology 2023Quote: ... tissue was scraped from each fragment (avoiding the apical tip) and added directly to 2 mL tubes containing 0.5ml DNA/RNA shield (Zymo Research) and Lysing Matrix A (MP Biomedicals ...
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bioRxiv - Genomics 2023Quote: ... at 37 °C for 2 hours and purified using Zymo ssDNA/RNA clean & concentrator kit following manufacturer’s instructions (Zymo D7011). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products with 150-400 bp were then cut and purified with 2% agarose gel and column (Zymo research, D4008). Finally ...
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bioRxiv - Microbiology 2023Quote: ... two agar plugs were placed in a 2 mL eppendorf tube with 500 μL of chloroform and agitated on a Genie Disruptor (Zymo) for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... two agar plugs were placed in a 2 mL eppendorf tube with 500 μL of chloroform and agitated on a Genie Disruptor (Zymo) for 2 min ...
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bioRxiv - Molecular Biology 2024Quote: ... All of these reactions generated a 181 bp amplicon that was cut out from a 2% agarose gel and cleaned using the Zymoclean Gel DNA Recovery Kit (Zymo).
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bioRxiv - Systems Biology 2024Quote: ... a commercial 1:1 mix of Imtechella halotolerans and Allobacillus halotolerans was added (2 × 107 cells each per 20 µL suspension; Zymo product numbers LMG 26483 and LMG 24826 ...
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bioRxiv - Developmental Biology 2020Quote: ... Extracted DNA was treated with RNAse A (1 μL of 1 mg/mL added to samples of 100 μL for 30 minutes at room temperature) and then purified and concentrated using a DNA Clean and Concentrator-5 Kit (Zymo Research Corp., USA). The concentration of genomic DNA was measured on a QUBIT 4 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 % ethanol was added to the aqueous phase (1:1, v/v) and transferred to RNA Clean and Concentrator (RCC) ™ −5 kit (Zymo Research; R1013) and centrifuged at 16,000 x g for 30 s ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from the sorted cells as described (Hrvatin et al., 2014a) and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1013). Complementary DNA (cDNA ...
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bioRxiv - Immunology 2021Quote: MSP1-specific B cells that successfully expanded in culture were collected by centrifugation (5 min. at 250 × g and RT) and stored at -70°C in 50 µl Tri-Reagent (Zymo #R2050-1-200). Heavy and light chain variable regions were amplified from MSP1-specific B cells by cDNA synthesis and a series of PCR reactions ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed from the 0.22 μm filters (containing the 5–0.22 μm microbial size fraction) using the ZR Soil Microbe DNA MiniPrep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions.