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Citations for Zymo Research :
1051 - 1100 of 1167 citations for 3 2 5 Dioxoimidazolidin 4 yl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... which were cut out from a 2% agarose gel and cleaned using the Zymoclean Gel DNA Recovery Kit (Zymo), eluting in 15 μl ...
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bioRxiv - Microbiology 2021Quote: ... cells collected by centrifugation (6000 xg, 10 min, 4°C) and DNA extracted using the ZR Fungal/Bacterial DNA Kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: DNA from 32 skull base chordomas and 4 NP samples were bisulfite converted with EZ-96 DNA Methylation kit (Zymo Research, Orange, CA, USA) and used for genome-wide DNA methylation profiling with Methylation EPIC (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated using the Illumina NextSeq 500 High Output Kit v2.5 (Cat. #20024908) and eluted in DNA Elution Buffer (Zymo Research, Cat. #D3004-4-10). Library concentration and average size was verified using the TapeStation DNA 5000 Assay Reagents and Screen Tapes (Agilent ...
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bioRxiv - Plant Biology 2020Quote: ... the 50 µL aliquot plus 5 µL of 10% (w/v) SDS was purified using the Zymo RNA clean and concentrator kit (Zymo Research; R1017). The total RNA was rRNA-depleted with the Ribo-Zero plant leaf kit (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... templates were run on a 1% agarose gel to confirm cutting and the reactions were purified with Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). 1ug of linearized template was used in a 100uL T7 RNA Polymerase (purified in house ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were run on a 1% agarose gel to check the product size and purified using Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). To create the cloning insert ...
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bioRxiv - Synthetic Biology 2021Quote: Plasmids were isolated from 5-10 mL yeast cultures either by alkaline lysis (39) or using Zymo Yeast Miniprep Kit I (Zymo Research D2001). Plasmids were then recovered into DH10B ElectroMax E ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Cell Biology 2022Quote: ... followed by selective recovery of total RNA using an RNA spin column clean-up kit (RNA Clean & Concentrator-5; Zymo, Irvine, CA). RNA was extracted from cultured cells by phase separation with chloroform followed by RNA precipitation in isopropanol as previously described [38] ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested codon random mRFP with singlestranded overhangs was cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). The complete 15 μl containing the codon random mRFP library was used in a ligation reaction to generate the plasmid library.
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bioRxiv - Molecular Biology 2022Quote: ... The ligation mixture was cleaned and concentrated to 10 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). 1 μl of the codon randomised mRFP library was transformed into electrocompetent DH10B cells (20 μl competent cells ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions containing the dsDNA block were cleaned and concentrated to 20 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 4 μl Gel Loading Dye ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using TRIzol-based (Fisher: 15596026) phenol-chloroform extraction and purified with RNA Clean and Concentrator-5 spin columns (Zymo research: R2050). RNA concentration and quality was assessed with a NanoDrop 1000 spectrophotometer (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were extracted from a 10 mL culture (5 OD) of sorted cells subpopulations using the Zymoprep™ Yeast Plasmid Miniprep II (Zymo Research) following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Biophysics 2021Quote: ... RNA was then extracted from the aqueous phase using a spin column-based purification kit (RNA Clean & Concentrator-5, Zymo Research # R1015) and reverse-transcribed into cDNA using SuperScript VILO Master Mix (Thermo Fisher # 11755050) ...
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was extracted from overnight cultures grown in 5 mL BHI broth using the Zymo BIOmics DNA Miniprep Kit (Zymo, Irvine, CA).
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bioRxiv - Genetics 2023Quote: ... Total RNA was purified from the aqueous phase using the RNA Clean & Concentrator-5 kit with DNase I treatment to remove genomic DNA contamination (Zymo Research R1013). RNA quantity was determined with the Qubit RNA Broad Range Assay (ThermoFisher Scientific Q10210) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (500 x g for 5 minutes) and lysed for RNA and DNA extraction using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research, D7003). RNA and DNA were eluted in water ...
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bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s instructions and 35 µL of the supernatant were treated with the RNA Clean & Concentrator Kit 5 (Zymo Research, Irvine, USA) using an adapted protocol (the RNA prep buffer step was performed twice ...
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bioRxiv - Cell Biology 2024Quote: ... and all reactions with the appropriate molecular weight product were combined and cleaned using the DNA Clean and Concentrator 5 kit (Zymo Research D4013). 10 µg LentiCRISPRv2-opti (Addgene #163126 ...
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bioRxiv - Neuroscience 2023Quote: ... The sequencing libraries were then run on a 10% TBE polyacrylamide gel and purified using the DNA Clean & Concentrator-5 kit (Zymo Research D4013).
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bioRxiv - Cancer Biology 2023Quote: ... The rRNA-depleted and DNAse-treated RNA was then cleaned with the Zymo RNA Clean and Concentrate-5 kit (Zymo Technologies, USA) and eluted in 8 uL nuclease-free water.
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bioRxiv - Biochemistry 2023Quote: ... The tagmented DNA was cleaned up using Zymo DNA Clean and Concentrator-5 columns with 5X binding buffer (Zymo, cat. no. D4004). 5 μL of purified DNA out of 12.5 μL purified product was amplified in a 25 μL PCR reaction containing NEBNext PCR master mix (1X final) ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmentation was performed for 30 minutes at 37°Con a rotator and DNA was purified using the DNA Clean and Concentrator 5 kit (Zymo Research D4004). PCR was performed as previously described [23] using 11 total cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were treated with 0.5 mg/ml proteinase K at 65 ℃ for one hour and purified with Zymo ChIP DNA Clean Concentrator kit (Zymo Research D5205) as the manufacturer’s manual and quantified by QUBIT ...
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bioRxiv - Molecular Biology 2024Quote: ... The aqueous phase was retained for RNA purification using the Zymo RNA Clean & Concentrator-5 kit following manufacturer directions for the capture of small RNA fragments with slight modification (Zymo Research, R1013). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... 75 μl of nuclease-free water was added to the mix followed by 270 μl of RNA binding buffer (RNA Clean and Concentrator-5 column kit, Zymo Research, R1015) and 400 μl of 100% ethanol ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were purified from agarose gels using the Zymoclean® Gel DNA Recovery Kit or from PCR reactions using the DNA Clean & Concentrator®-5 DNA kit from Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted by phenol-chloroform for the ZNF469 profiling to keep the small DNA fragments and with the DNA Clean & Concentrator-5 kit (Zymo Research, D4004) for the H3K27ac profiling ...
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bioRxiv - Microbiology 2024Quote: ... was amplified using flanking primers and the resulting PCR products were purified using a DNA Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA). STm 14028s carrying the pKD46 plasmid (64 ...
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bioRxiv - Molecular Biology 2021Quote: ... This library pool was then size selected on a 2% agarose gel by cutting between 150-800 nucleotides and performing gel purification (Zymo). To determine the loading density of the final pooled library ...
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bioRxiv - Microbiology 2020Quote: The Zymobiomics microbial community DNA standard consisting of 8 bacterial and 2 fungal genomic DNAs (cat. no. D6305) was obtained from Zymo Research (Irvine ...
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bioRxiv - Genomics 2022Quote: Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI, USA], 2 U/mL zymolyase [Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... Filters were then transferred into a 1.5 ml tube and the filter-associated cells were resuspended by adding 1 ml 1X PBS + 0.05% Triton X-100 detergent and agitating the tubes at high speed for 2 minutes using a Genie Disruptor (Zymo). This suspension as well as the inoculum were diluted ...
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bioRxiv - Genomics 2021Quote: ... RNA was extracted from inactivated swab samples using the Quick DNA/RNA Viral Magbead kit from step 2 of the DNA/RNA purification protocol (Zymo) (files.zymoresearch.com/protocols/_r2140_r2141_quick-dna-rna_viral_magbead.pdf).
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bioRxiv - Developmental Biology 2023Quote: DNA was extracted from fin clips stored in 100% ethanol for <2 weeks using the PCR biosystems rapid extract lysis kit or Quick-DNATM Miniprep Plus kit by Zymo research ...
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bioRxiv - Microbiology 2023Quote: ... two agar plugs were placed in a 2 mL eppendorf tube with 500 μL of chloroform and agitated on a Genie Disruptor (Zymo) for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... two agar plugs were placed in a 2 mL eppendorf tube with 500 μL of chloroform and agitated on a Genie Disruptor (Zymo) for 2 min ...
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bioRxiv - Genomics 2023Quote: ... at 37 °C for 2 hours and purified using Zymo ssDNA/RNA clean & concentrator kit following manufacturer’s instructions (Zymo D7011). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products with 150-400 bp were then cut and purified with 2% agarose gel and column (Zymo research, D4008). Finally ...
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bioRxiv - Microbiology 2023Quote: ... tissue was scraped from each fragment (avoiding the apical tip) and added directly to 2 mL tubes containing 0.5ml DNA/RNA shield (Zymo Research) and Lysing Matrix A (MP Biomedicals ...
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bioRxiv - Molecular Biology 2024Quote: ... All of these reactions generated a 181 bp amplicon that was cut out from a 2% agarose gel and cleaned using the Zymoclean Gel DNA Recovery Kit (Zymo).
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bioRxiv - Systems Biology 2024Quote: ... a commercial 1:1 mix of Imtechella halotolerans and Allobacillus halotolerans was added (2 × 107 cells each per 20 µL suspension; Zymo product numbers LMG 26483 and LMG 24826 ...
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bioRxiv - Developmental Biology 2020Quote: ... Extracted DNA was treated with RNAse A (1 μL of 1 mg/mL added to samples of 100 μL for 30 minutes at room temperature) and then purified and concentrated using a DNA Clean and Concentrator-5 Kit (Zymo Research Corp., USA). The concentration of genomic DNA was measured on a QUBIT 4 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 % ethanol was added to the aqueous phase (1:1, v/v) and transferred to RNA Clean and Concentrator (RCC) ™ −5 kit (Zymo Research; R1013) and centrifuged at 16,000 x g for 30 s ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from the sorted cells as described (Hrvatin et al., 2014a) and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1013). Complementary DNA (cDNA ...