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Citations for Zymo Research :
1001 - 1050 of 1137 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... The IVT reaction was assembled based on the manufacturer’s recommendations and the sgRNA was purified using an RNA Clean & Concentrator-5 kit (Zymo Research). RNA concentration was measured using a NanoDrop (ThermoFisher)
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bioRxiv - Plant Biology 2021Quote: ... The RNA was then purified and concentrated using the RNA Clean Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). cDNA libraries were prepared using a mix of 50 ng RNA and 0.5 ng Spike-in RNA Variant Control Mix E2 (Lexogen ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated using the PerfectPure RNA Cultured Cell kit (5 PRIME) or Quick-RNA™ MiniPrep (Zymo Research). One microgram of the total RNA was reverse-transcribed into cDNA with M-MLV (Promega ...
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bioRxiv - Immunology 2021Quote: ... Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo). Fragments were amplified according to the previously published protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA fragments were purified from the reaction using a RNA Clean & Concentrator(tm)-5 column (Zymo Research, Cat. n° R1013). The resulting RNA fragments were ligated to different adaptors (listed in Supplementary Table 1 ...
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bioRxiv - Genetics 2020Quote: ... viral RNA was prepared from 200 µl of clarified virus stocks (3.000 rpm, 5 min) with the Quick-RNA Viral Kit (Zymo research) and eluted in 14 µl RNase free H20 ...
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bioRxiv - Genetics 2021Quote: RNA was isolated from eight 5 days old males using ZR Tissue and Insect RNA MicroPrep™ (Zymo Research, #R2030). DNA was removed using TURBO™ DNase (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... RNA was cleaned and concentrated to 10 μl using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA), as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... P7 and E18.5 was directly dissolved in RNA isolation buffer and RNA was isolated using the Quick-RNA micro prep kit (Zymo). Library preparation was done using the NEB Next® Ultra™ RNA Library Prep Kit ...
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bioRxiv - Cell Biology 2022Quote: ... all wells were pooled to purify DNA products using the Zymo DNA Clean & Concentrator-5 kit (Zymo, Cat #: 11-303) according to manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: RNA was extracted from various developmental stages and adult tissues using TRIzol Reagent (Thermo Fischer Scientific, Waltham, MA, USA) and further purified with the RNA clean and concentrator-5 kit (Zymo), following the provided instructions ...
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bioRxiv - Genetics 2023Quote: ... For specific miRNA quantification, we size-fractionated small RNAs (< 200nt, containing pre-miRNAs) using RNA Clean & Concentrator 5 column kits (Zymo), as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were treated with 1 μL of DPNI for 3 h at 37°C and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s protocol (Zymo CN# D4004).
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and DNA was extracted at days 1 and 5 utilizing the Quick-DNA/RNA Miniprep (Zymo Research). Viral genomes were quantified using a standard curve and normalized to RNAseP.
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bioRxiv - Microbiology 2023Quote: Total DNA was isolated from ∼6x105-7x105 MRC-5 infected fibroblasts using Zymo Duet RNA/DNA isolation Kit (Zymo Research). Viral genomes were quantitated by quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... PCR purification and plasmid isolation was done using the DNA Clean & Concentrator-5 and Zyppy Plasmid Miniprep Kits from Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR product sizes were verified on a 1% agarose gel prior to PCR clean up (DNA Clean & Concentrator-5, Zymo). A DNA fragment for transcription of lpp (lpp sequence preceded by aforementioned T7 transcription-enabling sequence ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted and purified using Trizol reagent and RNA Clean & Concentrator-5 kit (Zymo Research cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The cells debris was removed after centrifugation at 15000g for 5 mins and cell supernatant containing the released virions were subjected to 5μl DNase I (Zymo research) for 1 hour to remove remaining untransfected chimeric NL plasmid ...
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bioRxiv - Genomics 2023Quote: ... the aqueous phase was transferred to an RNA Clean and Concentrator-5 column and purified according to the manufacturer’s instructions including on-column DNaseI digestion (Zymo Research).
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bioRxiv - Biochemistry 2022Quote: ... decorated cDNA from multiple samples were pooled together and purified using the DNA Clean & concentrator-5 Kit (Zymo research, #D4014). After elution with 20 µl of nuclease free water ...
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bioRxiv - Biochemistry 2023Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... treated with 1 μL of DpnI for 3 h at 37℃ and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo CN# D4004). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Microbiology 2024Quote: ... The reaction was stopped by adding 250 µl of DNA Binding Buffer and DNA was isolated using the Clean and Concentrator-5 Kit (Zymo). Libraries were produced by PCR amplification of tagmented DNA and sequenced on a NextSeq 2000 sequencer 150 bp Paired End.
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bioRxiv - Microbiology 2024Quote: ... beads were removed by centrifugation (20,000 × g, 5 min) and total RNA was isolated using the Direct-zol RNA miniprep kit (Zymo Research) as directed by the manufacturer.
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bioRxiv - Microbiology 2024Quote: ... The 60 samples were pooled into 5 groups of 12 samples each during purification using RNA Clean & Concentrator (Zymo Research) and all eluted with 16µl DEPC UltraPure water.
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bioRxiv - Molecular Biology 2024Quote: ... a final round of PCR was performed by taking 100 ng of amplified sgRNA library and 5 cycles of the above PCR program was run followed by Zymo column clean up ...
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bioRxiv - Immunology 2024Quote: ... and the resulting products were purified using the DNA Clean and Concentrator-5 Kit following the manufacturer’s instructions (Zymo Research). In-Fusion reactions were prepared in 5 µL volumes at a 1:3 vector to insert molar ratio ...
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bioRxiv - Biochemistry 2024Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Bioengineering 2024Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Molecular Biology 2022Quote: ... TurboDNase treatment as described above usually removes most genomic DNA and small fragments of DNA that could prime as random primers (detectable by ‘no RT primer’ control) are cleared away efficiently by the RNA Clean & Concentrator-5 kit (Zymo Research). Hence ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from 150 µL of the fractionated samples using an RNA clean and concentrator-5 kit (Zymo Research, R1016) using the same procedure to extract RNA from unfractionated cell lysate (described above) ...
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bioRxiv - Molecular Biology 2021Quote: ... purified PCR product was digested with EcoRI-HF and BamHI-HF at 37°C for 1.5 hours and purified again using a Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). For the cloning backbone ...
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bioRxiv - Genetics 2021Quote: ... 5 heads of each genotype were pooled and total RNA extracted using the Direct-zol RNA Microprep kit (Zymo Research, R2060) and the quality determined using a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Genetics 2020Quote: ... all PCR products were cleaned using a Zymo Research ZR-96 DNA Clean & Concentrator™-5 (Zymo Research, Irvine, California, USA). Samples were eluted in 50µl of DNA free water.
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bioRxiv - Bioengineering 2020Quote: ... at 37°C for 24 hours to ensure complete removal of any 5’-triphosphate moieties then purified again using a RNA spin column (Zymo Research). Lastly ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared supernatant was then transferred to a fresh reaction tube and applied to spin-columns (RNA Clean & Concentrator-5 columns, Zymo-Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The reaction was then treated with DNase I to remove template DNA and RNA product was purified using an RNA Clean and Concentrator-5 (Zymo #R1016). Purified RNA was used as the template for reverse transcription using ProtoScript II according to manufacturer protocol ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the DNA template via in vitro transcription (IVT ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA precipitants were treated with 100 μl of 0.04 U Turbo DNase I at 25°C for 30 min to remove DNA contamination and cleaned using an RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Research, USA). The RNA was eluted in 16 μl RNase-free water for subsequent experiments ...
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bioRxiv - Cell Biology 2021Quote: ... the RNA was cleaned to remove enzyme and buffer components using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1014) before the next treatment ...
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bioRxiv - Microbiology 2021Quote: ... rRNA depleted RNA was purified from the reaction mixture with the Zymo RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA) according to manufacturer instructions and eluted in 12 µl nuclease-free water ...
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bioRxiv - Synthetic Biology 2021Quote: ... After the second treatment with DNase I the samples were column purified and concentrated using RNA Clean & Concentrator-5 kit (Zymo Research). Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... The digested fragments were purified using the DNA Clean & Concentrator-5 Kit and the Zymoclean Gel DNA Recovery Kit (Zymo Research) respectively ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Mixture was incubated at 37 ℃ for 30 min followed by purification with a Zymo DNA Clean and Concentrator-5 Kit (Zymo, DP4033). Transposed DNA was eluted in 24 μl elution buffer and amplified by PCR reaction (10 μl 5 X TAB ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-5 and LPI-15 were collected from three greenhouse-grown WT plants (∼5 ft in height) for RNA extraction using Direct-zol RNA MiniPrep kit (Zymo Research) with Plant RNA Purification Reagent (Invitrogen) ...