Labshake search
Citations for Zymo Research :
1001 - 1050 of 1371 citations for 2' Chloro 4 5 5 dimethyl 1 3 dioxan 2 yl butyrophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were resolved on 2% agarose gels followed by extraction and purification of candidate DNA amplicons using Zymoclean Gel DNA Recovery Kits (Zymo Research). Purified PCR products were cloned using the TOPO TA cloning kit (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e. 1X PBS, pH 7.2 or Zymo DNA/RNA shield). Swabs in PBS were placed on ice and sent for culturing while the other swabs were transported back at room temperature to the laboratory and stored at -80°C ...
-
bioRxiv - Plant Biology 2020Quote: ... Amplicons were excised from a 2 % agarose gel (200-800 bp fragments were excised) and purified using the Gel DNA recovery kit (Zymo Research). Library concentrations were measured with qPCR according to Illumina’s Sequencing Library qPCR Quantification Guide ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Products were loaded on a 2 % agarose gel and the appropriate bands were excised and cleaned up with the Zymoclean Gel DNA Recovery Kit (Zymo Research). All purified amplicons were shipped to Macrogen Europe B.V ...
-
bioRxiv - Genomics 2021Quote: ... Chronotype was assessed by computing mid-sleep time on free days (MSF).2 Participants’ stool samples were collected using DNA/RNA Shield Fecal Collection tubes (Zymo research). Sample DNA extraction was performed using the PureLink Microbiome DNA Purification Kit (Invitrogen ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... 300µm diameter) follwing a Proteinase K digestion (at 55°C for 2 hours) using a Quick-RNA MicroPrep kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... the reaction was prepared in a volume of 20 µL consisting in 2 µL of the template DNA and 18 µL of Femto Bacterial qPCR Premix (Femto Bacterial DNA Quantification Kit, Zymo Research). The PCR reaction was performed using a 7500 Fast Real-Time PCR System (ThermoFisher Scientific ...
-
bioRxiv - Genomics 2024Quote: Isolates for sequencing were cherrypicked using an Opentrons OT-2 Liquid Handler and DNA extraction was then performed using the Quick-DNA Fecal/Soil Microbe Kit (Zymo Research) on a KingFisher Flex instrument (Thermo Fisher Scientific) ...
-
bioRxiv - Systems Biology 2023Quote: ... Pools 4-8 from experimental replicate #2 were resuspended in 100 µL spheroplast digest buffer with 2.5U/mL Zymolyase T100 (Zymo Research #E1005) and incubated at 37°C for 20 minutes ...
-
bioRxiv - Genetics 2022Quote: RNA was extracted from pellets of ∼2 million iPSCs or ∼6 million neurons immediately after harvesting with the Quick-RNA Miniprep Kit (Zymo R1054) following manufacturer’s instructions except for two alterations ...
-
bioRxiv - Cell Biology 2023Quote: ... before being spun down (10k rpm, 2 minutes) and resuspended in 80 μL of digestion buffer (included in the YeaStar RNA Kit, Zymo Research). Cells were then stored on ice until ready for RNA extraction ...
-
bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Genomics 2024Quote: ... for 2 hr at 37 C and then cleaned and concentrated using a Zymo DNA Clean and Concentrator column (Zymo D4013). The debranched RCA product was run on a 1% low-melt agarose gel ...
-
bioRxiv - Plant Biology 2020Quote: ... a qRT-PCR product was purified from a complete PCR reaction using the Zymo DNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA) and eluted in 10 μl of nuclease-free water ...
-
bioRxiv - Microbiology 2021Quote: ... Genomic DNA extractions were performed upon 5 million trophozoites of Nf69 using Quick-DNA Miniprep Plus Kit according to manufacturer recommendations (Zymo Research, Irvine, CA, USA). In short ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The sample was then centrifuged at 2300 rpm for 5 min and the supernatant was collected and concentrated and purified using a Zymo-Spin V reservoir (Zymo Research Irvine, CA, USA) and Qiagen MinElute spin column (Qiagen ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... strains were struck to synthetic defined (SD) media (CSM minus arginine, yeast nitrogen base, glucose) containing 5-Fluoroorotic Acid (5FOA) (Zymo Research, Irvine, CA, USA). to isolate cells that spontaneously lost pTC416-SEC53 ...
-
bioRxiv - Neuroscience 2023Quote: RRBS libraries were prepared from 200 ng of genomic DNA digested with MspI restriction enzyme and then extracted with ZR-DNA Clean & Concentrator™-5 kit (Zymo Research, Cat# D4014). According to Illumina’s specified guidelines ...
-
bioRxiv - Microbiology 2023Quote: ... a 4.5 ml sample of each microcosm and extracted bacterial DNA with a Quick-DNA Fecal/Soil Microbe Miniprep Kits (Zymo Research, Irvine, California, USA). Two negative controls that contained the SH salt medium alone were included in the process of DNA extraction ...
-
bioRxiv - Neuroscience 2020Quote: ... and sorted into 384-well plates preloaded with 2μl of digestion buffer for snmC-seq215 (20 mL digestion buffer consists of 10 mL M-digestion buffer (2×, Zymo D5021-9), 1 ml Proteinase K (20 mg ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was extracted from SARS-CoV-2 infected and uninfected Huh7 cells using the Direct-zol™ RNA Miniprep (Zymo Research, USA).
-
bioRxiv - Microbiology 2021Quote: RNA from homogenized virus-inactivated lung and brain tissues of SARS-CoV-2 infected animals was extracted using the Direct-zol RNA MiniPrep Kit (Zymo Research R2051) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... RNA from SARS-CoV-2 infected and uninfected Huh7 cells and supernatent was extracted using the Direct-zol RNA Miniprep (Zymo Research, US) and quantitative real-time polymerase chain reaction (qRT-PCR ...
-
bioRxiv - Neuroscience 2021Quote: ... cultured neurons at 15 DIV were left untreated or treated for 48 h with 2 μM TTX and then processed for total RNA extraction using the kit Direct-zol™ RNA MicroPrep (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... C-SCCE0625), and ScriptCap 2′-O-Methyltransferase (Cellscript, C- SCMT0625) and purified by a Direct-zol RNA Microprep Kit (Zymo Research, D2060). The radiolabeled TOP RNAs (4 pmol ...
-
bioRxiv - Immunology 2021Quote: ... followed by a final clean-up step by agarose-gel extraction (2% (w/v) gel) with Zymoclean Gel DNA Recovery kit (Zymo Research D4001). Yeast scFv display libraries were generated using the amplified VH and VL DNA libraries from above and the pYD1 yeast surface display vector (Addgene plasmid #73447 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... the clarified cell lysates (2×108 cells) were used to purify total RNA using the Direct-zol RNA MiniPrep kit (Zymo Research, USA). One microgram of total RNA after Turbo DNase I (Thermo Fisher Scientific ...
-
bioRxiv - Zoology 2023Quote: ... cucullus vegetative cells (300,000 cells) and 2-week-old mature cysts (3,000,000 cells) using the Direct-zol RNA purification system (Zymo Research Crop., California, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from HL-7702 cell lines and 2 μg DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (ZYMO Research, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... 2 μl of 0.5 M EDTA was added to this mixture and RNA fragments were column purified (Zymo research Cat no-D4061) and eluted in RNAse free water ...
-
bioRxiv - Microbiology 2020Quote: ... The final pool volume was reduced to concentrate the sequencing library using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). The pooled library concentration was determined using the Quant-iT™ High-Sensitivity DNA Assay Kit (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... we prepared a series of 8 serial dilutions of a known mock microbial community consisting of 8 gram positive and negative bacteria and 2 yeast (Zymo Research, catalog # D6300), as previously described (31) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was extracted from 2-10 mL of stationary cultures with the Zymo Research Midiprep gDNA kit (Zymo Research EUROPE GmbH, Germany). Library preparation and sequencing were performed at Novogene (UK ...
-
bioRxiv - Genomics 2023Quote: ... Purification was performed according to manufacturer’s instructions and the converted DNA was then eluted in 32.5 µl nuclease-free water (17 µl × 2 times) through a column from the DNA Clean & Concentrator kit (Zymo Research, cat. no. D4003).
-
bioRxiv - Zoology 2023Quote: ... for 3 min at 28 Hz in 2 mL microcentrifuge tubes in 1X sterile PBS or four volumes of DNA/RNA Shield (Zymo Research, Irvine, CA) and two 5 mm stainless steel grinding balls ...
-
bioRxiv - Plant Biology 2024Quote: Each composite sample was placed in a 2 ml ZR BashingBead Lysis tube containing beads and ZR stabilization reagent (Zymo Research, Irvine, CA), and was mixed immediately in the field using a bead-beating TerraLyzer lysis system (Zymo Research ...
-
bioRxiv - Genomics 2024Quote: ... and 2 µL of DNA isolated using the Quick-DNA Fecal-Soil Microbe kit or the Quick-DNA Miniprep kit (Zymo Research, Irvine, CA). Reactions were run in a BioRad C1000 Touch (Bio-Rad Laboratories ...
-
bioRxiv - Bioengineering 2024Quote: ... we first made a PCR standard by gel extracting the 93-bp PCR amplicon produced from the GAPDH primer/probe set using a 2% agarose gel and Zymo DNA Gel Extraction kit (Zymo cat. no. D4007). The concentration of gel-extracted GAPDH amplicon was quantified with a QuBit high sensitivity dsDNA assay and then diluted in nuclease-free water to create 10-fold serial dilutions ranging from 1e9 to 1e2 molecules/uL ...
-
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... Punches from each of these brain regions were placed in individual tubes containing 2□mm size beads (ZR BashingBeads Lysis Tubes, Zymo Research, item S6003-50) and 1 mL of QIAzol lysis reagent (Qiagen ...
-
bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: RNA was isolated from the cell culture supernatant of SARS-CoV-2-infected cells using the Quick-RNA Viral Kit (Zymo, California, USA cat no. R1035) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: The expression vectors were introduced into the yeast PichiaPink™ Strain 2 using Frozen-EZ Yeast Transformation II™ (Zymo Research Co., Irvine, CA, USA). Recombinant strains were initially cultured in BMGY liquid medium at 30 °C for about 24 h ...
-
bioRxiv - Microbiology 2023Quote: ... containing a known concentration and taxonomy of 8 bacterial and 2 fungal species was also included as an internal control (Zymo Research Corp, Cat No. D6310). Extracted nucleic acids from each sample were randomized into a 96-well rack (Figure 1C ...
-
bioRxiv - Physiology 2024Quote: ... the column was brought to room temperature for 5 min and the RNA was spun out into fresh tubes (50 μL for Zymo-II and 60 μL for Zymo-IICG columns). Next ...
-
bioRxiv - Neuroscience 2023Quote: OE from 3 controls and 3 sFlt1 mice was dissected and immediately placed in RNA lysis buffer (Zymo Research R1051) without vortexing for 5min and further processed according to manufacturer’s guidelines ...
-
bioRxiv - Microbiology 2023Quote: ... 4 ml of urine was added to 4 ml of 2X DNA/RNA Shield® (Zymo, USA). Serum and urine samples were stored and transported on ice to the Institute of Microbiology at Universidad San Francisco de Quito and thereafter maintained at −20°C until DNA extraction.
-
bioRxiv - Evolutionary Biology 2020Quote: Total RNA from six whole 20-21 dpf offspring (3 control offspring, 3 treatment offspring) was extracted using a Zymo Duet extraction kit (Zymo, NZ). The integrity of RNA samples was determined using an Agilent RNA 6000 Nano chip on an Agilent 2100 Bioanalyzer to check that the samples had an RNA Integrity Number (RIN ...
-
bioRxiv - Genomics 2020Quote: ... DNA in the initial 20-μL nucleic acids solution was digested by adding 16-μL DNase I (1 U/μL) plus 4-μL 10X DNase I buffer (DNase I set; Zymo Research), incubating for 15 min at room temperature ...