Labshake search
Citations for Zymo Research :
951 - 1000 of 1307 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... We cleaned and concentrated the reaction product using a DNA Clean & Concentrator-5 Kit (Zymo, #D4014) and eluted in 25 μL MB H2O ...
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bioRxiv - Genetics 2023Quote: ... and PCR products were cleaned up using the DNA Clean & Concentrator-5 kit (Zymo Research #D4014). We later quantified the concentration of PCR fragments using a Nanodrop (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Precipitated RNA solutions were transferred to Zymo RNA columns (Zymo RNA Clean & Concentrator 5 kit, R1013) for DNAse treatment and purification as per manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 75 µl DNA Digestion Buffer and 5 µl DNAase (Zymo Research, USA, CA, Cat No: E1010) were added to the dried pellet ...
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bioRxiv - Microbiology 2024Quote: ... followed by a cleaning step using RNA Clean & ConcentratorTM-5 kit (Zymo Research, Irvine, CA, USA) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... The product was then purified using the DNA Clean and Concentrator-5 kit (Zymo Research, USA). These amplified copies of the original DNA isolations contain no DNA modifications ...
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bioRxiv - Microbiology 2024Quote: ... The DNA-free RNA was cleaned up with the RNA Clean & Concentrator-5 kit (Zymo Research) and eluted in 15 µl of water ...
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bioRxiv - Molecular Biology 2024Quote: ... The final libraries were cleaned up using Zymo DNA Clean & Concentrator kit 5 (Zymo Research, D4014). KAS-seq libraries were pooled and sequenced on an Illumina HiSeq 4000 System (University of Manchester Genomic Technologies Core Facility) ...
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bioRxiv - Biophysics 2024Quote: ... Linearized plasmid DNA was immediately purified using the DNA Clean and Concentrator-5 kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... RNA was purified from the reaction mixture using the RNA Clean and Concentrator-5 kit (Zymo) and eluted in 20 μL of water.
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bioRxiv - Synthetic Biology 2024Quote: ... Approximately 700-800 µl of the mixture was transferred to a column-IC (Zymo C1004-5), washed twice with 700 µl and 400 µl RNA wash buffer for the first and second wash ...
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bioRxiv - Molecular Biology 2024Quote: ... The probed RNAs were then cleaned using the recommended RNA Clean and Concentrator-5 protocol (Zymo).
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bioRxiv - Genomics 2024Quote: ... the cleanup reaction was performed with a Zymo DNA Clean and Concentrator-5 Kit (D4014, Zymo). Library preparation with eluted genomic DNA can be performed using TruePrep DNA Library Prep Kit (TD501 ...
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bioRxiv - Zoology 2024Quote: ... The RNA was then cleaned using the Zymo RNA Clean and Concentrator -5 kit (Zymo, R1015) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted and purified using the RNA Clean & Concentrator-5 Kit (Zymo Research, Irvine, CA). 1.5 ug RNA was converted to cDNA using the SuperScript III RT-PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... RNA purification was performed using RNA Clean and Concentrator-5 kit (Zymo Research; Irvine, CA, USA) as per the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: DNA bisulfite conversion was performed directly starting from cell pellets (a maximum of 1×105 cells per sample) using the EZ DNA Methylation-Direct kit (Zymo Research #D5021) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The corresponding amount of polyA RNA were incubated with 1X fragmentation buffer at 95 °C for 1 minute to generate ~300 nt of RNA fragment followed by column purification (Zymo Research, R1016) as described before [23] ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from three biological replicates of E/I networks composed of Ngn2 #2 and Ascl1 #1 (DIV 49) with the Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Total RNA from adipose tissues (experiment 1) was extracted using the Direct-zol™ RNA MiniPrep Plus kit protocol (Zymo research, #R2071). RNA was reverse-transcribed using the SuperScript™ III Reverse Transcriptase Kit (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
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bioRxiv - Genetics 2020Quote: ... DNA was digested in columns by adding a mixture of 5 μl RNA Lysis Buffer 1 from the kit andl DNase I and 75 μl RNA Lysis Buffer 1 from the kit andl DNA Digestion Buffer from DNase I Set kit (Zymo Research E1010) followed by a 15-minute incubation at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Genomic DNA (1 μg) was treated with sodium bisulfite using the EZ DNA Methylation Gold Kit per manufacturer’s instructions (Zymo Research; Irvina, CA). Bisulfite converted DNA (∼20 ng ...
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bioRxiv - Immunology 2021Quote: ... PCR products were subjected to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: DNA bisulfite conversion was performed starting from a maximum of 1 × 105 pelleted cells per sample using the EZ DNA Methylation-Direct kit (Zymo Research #D5021), and following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The amplified promoter and coding PIGJ DNA sequence were first purified from 1% agarose gels using the ZymocleanTM Gel DNA recovery kit (Zymo Research, #D4007) and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were grown in Sc-Ura medium for 1 d and plasmid DNA was purified using Zymoprep II Yeast plasmid Miniprep kit (Zymo Research, CA). Plasmids were then transformed into E ...
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bioRxiv - Plant Biology 2023Quote: ... The adapter-ligated DNA molecules were purified using 1× Agencourt AMPure XP magnetic beads and were subjected to bisulfite conversion using ZYMO EZ DNA Methylation-Gold Kit (Zymo, Cat. #D5005). Libraries were then amplified by PCR using 20 μL of bisulfite-converted DNA molecules ...
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bioRxiv - Microbiology 2024Quote: ... at which time the 0 minute timepoint aliquots were rapidly collected as follows: 200 μL of culture was mixed with 1 mL of DNA/RNA shield (Zymo Research Corporation) to be used for RNA extraction (described later ...
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bioRxiv - Cell Biology 2024Quote: ... the beads were boiled in Tris-EDTA containing 1% SDS and 12% β-Mercaptoethanol before RNA was extracted using a Direct-zol RNA Microprep kit (Zymo Research). The extracted RNA was then prepared for RT- qPCR analysis to examine RB1 ...
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bioRxiv - Physiology 2024Quote: ... WT and Rnlsmut NIT-1 cells were used for RNA isolation using Zymo Quick-RNA miniprep plus kit (Zymo Research cat. R1058). Novogene Corporation Inc ...
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bioRxiv - Immunology 2024Quote: ... coli were resuspended in 1 ml of TRIzol and their RNA was purified using Quick-RNA DirectZol columns ((#ZR2072 Zymo Research Ozyme) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... individual array bearing worms were isolated and lysed in 4µl of EB (Zymo, Cat. No.: D3004-4-16) buffer with 1mg/ml proteinase K (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... hydrophila MAH-4 was pelleted and genomic DNA obtained using Zymo DNA Miniprep (Zymo Research, Irvine, CA, USA). Long reads and short paired end reads (2 x 151 bps ...
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bioRxiv - Genetics 2023Quote: ... we extracted DNA from 2–4 dpf whole larvae using the Quick-DNA Microprep Kit (D3021, Zymo Research). We then performed a PCR for GFP as well as a ‘junction PCR’ to amplify a region including both mitfa and GFP to ensure integration at the intended locus.
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bioRxiv - Molecular Biology 2024Quote: ... 4°C) and DNA contained in the supernatant was extracted using the DNA Clean & Concentrator kit (Zymo D4013). The fragment sizes were assessed by Agilent BioAnalyzer to choose the appropriate MNase concentration yielding a 90/10 monomer/dimer ratio.
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bioRxiv - Genomics 2024Quote: ... worms were thawed at 4°C and subsequently preserved in DNA/RNA Shield (Zymo Research, Irvine, CA, USA). Adults of T ...
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bioRxiv - Cancer Biology 2022Quote: ... Individual 50 μL reactions were run on a 3% agarose gel and libraries were purified using the Zymoclean Gel DNA Recovery kit (D4007, Zymo Research). Sequencing was performed using standard Illumina instructions ...
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bioRxiv - Microbiology 2022Quote: ... and stored at -20°C until (≤ 3 days) the DNA was extracted via the Zymo Quick-DNA Fecal/Soil Microbe kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2021Quote: ... The nascent RNA was purified by adding 3 volumes of TRIzol and processed with the Direct-zol RNA Miniprep kit (Zymo Research) with addition of 1/100 volume of 100 mM DTT to each supplied wash buffer.
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bioRxiv - Microbiology 2021Quote: ... and genomic DNA was extracted from 3 mL of overnight (TSB) broth culture using the ZR Duet DNA/RNA MiniPrep Plus kit (Zymo Research) following manufacturer’s protocol for the “suspended cells” option ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Microbiology 2022Quote: ... The high molecular weight bands were excised with a razor and dissolved in 3 volumes of Zymo Agarose Dissolving Buffer (Zymo D4001) at 50°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared supernatant was discarded and the concentrated high-speed pellet (“100,000 x g pellet”) was lysed directly in 300 μl of TRI reagent (Zymo Research, R2050-1-200). RNA extraction was performed according to instructions for the Direct-zol RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Immunology 2021Quote: ... mice were euthanized with isoflurane overdose and one lobe of lung tissue was collected in an Omni Bead ruptor tube filled with Tri Reagent (Zymo, #R2050-1-200). Tissue was homogenized using an Omni Bead Ruptor 24 (5.15 ms ...
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bioRxiv - Molecular Biology 2022Quote: ... Two-third of final flag elute was taken for DNase treatment using NEB DNase 1 (M0303) and RNA was purified using RNA Clean and Concentrator Kit (Zymo Research, R1015/R1017). The final RNA concentration was determined using Qubit RNA high Sensitivity Assay Kit and 100ng of RIP-purified RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α λpir (Table 1) was prepared using the commercially available Mix and Go™ transformation kit (Zymo Research International, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified samples were run on a 2% agarose gel and fragments of the correct size were purified using ADB Agarose Dissolving Buffer (Zymo D4001-1-100) and UPrep Micro Spin Columns (Genesee Scientific 88– 343) ...
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bioRxiv - Microbiology 2022Quote: ... 2 extracted with tissue samples and 1 run with water samples) and 2 samples of DNA from mock bacterial communities (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Irvine CA) were prepared and sequenced ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research, Irvine, CA). The purified DNA fragments were then submitted for Sanger sequencing (GENEWIZ ...