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Citations for Zymo Research :
51 - 100 of 336 citations for 7 Oxa 3 azabicyclo 4.1.0 heptane 1 methyl 3 propyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Genetics 2019Quote: 25-30 eyes were dissected from both WT and pitx2ΔCE5-7 eye zebrafish embryos at 3 dpf in Ringer buffer and genomic DNA was purified using Quick-DNA microprep Plus kit (ZYMO Research). Eyes were resuspended in Solid Tissue buffer containing Proteinase K and incubated at 55°C for 6 hours ...
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bioRxiv - Microbiology 2019Quote: ... at 48 hpe cells were washed 3 times with PBS to eliminate extracellular virus and total RNA was extracted using Direct-zol RNA MiniPrep Plus (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Individual 50 μL reactions were run on a 3% agarose gel and libraries were purified using the Zymoclean Gel DNA Recovery kit (D4007, Zymo Research). Sequencing was performed using standard Illumina instructions ...
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bioRxiv - Microbiology 2022Quote: ... and stored at -20°C until (≤ 3 days) the DNA was extracted via the Zymo Quick-DNA Fecal/Soil Microbe kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... the DNA was treated with sodium bisulfite under non-denaturing conditions (no denaturation and incubation at 37°C for 3 hrs) using the Zymo Lightning bisulfite modification kit (Zymo Research). Following sample clean-up ...
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bioRxiv - Genomics 2021Quote: ... The nascent RNA was purified by adding 3 volumes of TRIzol and processed with the Direct-zol RNA Miniprep kit (Zymo Research) with addition of 1/100 volume of 100 mM DTT to each supplied wash buffer.
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bioRxiv - Microbiology 2021Quote: ... and genomic DNA was extracted from 3 mL of overnight (TSB) broth culture using the ZR Duet DNA/RNA MiniPrep Plus kit (Zymo Research) following manufacturer’s protocol for the “suspended cells” option ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Microbiology 2022Quote: ... The high molecular weight bands were excised with a razor and dissolved in 3 volumes of Zymo Agarose Dissolving Buffer (Zymo D4001) at 50°C ...
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bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Immunology 2022Quote: ... Libraries for Illumina-based sequencing were prepared with the Pico Methyl-Seq Library Prep Kit (Zymo Research) using Illumina TruSeq DNA methylation indices ...
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bioRxiv - Neuroscience 2023Quote: Sorted nuclei DNA on beads were processed by Pico Methyl-Seq Library Prep Kit (Zymo Research, D5455) to generate bisulfite sequencing libraries ...
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bioRxiv - Genomics 2020Quote: ... The genomic DNA of JLat cells and CD4 T cells from individuals #3 and #4 were extracted with a Quick-DNA Miniprep Plus kit (Zymo Research #D4068) according to the product protocol ...
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bioRxiv - Genomics 2022Quote: ... into the 20 μl ligation reaction for incubation with 30 minutes at 30°C and 3 minutes at 65°C to inactivate the enzymes followed by column purification (Zymo Research, R1016). Before the reverse transcription (RT) ...
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bioRxiv - Plant Biology 2020Quote: Genomic DNA was extracted from aerial parts of 3-week-old seedlings using Quick-DNA Plant/Seed Miniprep Kit (Zymo research, D6020) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: A PCR of all constructs using forward primer pTD2F (5’-TTGGCACCAAAATCAACGGG–3’) and reverse primer SP6 was carried out with SuperFi DNA Polymerase (ThermoFischer Scientific, USA) and agarose gel purified (Zymo Research, USA). These primers amplify the gene insert ...
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bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
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bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
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bioRxiv - Zoology 2024Quote: ... Clean-up used a combination of two kits: the beads from the Select-a-size Magbead kit (Zymo Cat# D4084-3-10) and the buffers from Genomic DNA Clean & Concentrator -25 (Zymo Cat# D4064) ...
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bioRxiv - Physiology 2024Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick-RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Physiology 2023Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick- RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Neuroscience 2019Quote: ... Methylation-sensitive restriction enzyme-dependent methylation analysis was performed using OneStep q-Methyl Kit (Zymo, Irvine, CA, USA) following the instructions given in the manual using 20ng DNA for each sample ...
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bioRxiv - Plant Biology 2021Quote: ... Bisulfite converted DNA was used to build libraries with the Pico Methyl-Seq library kit (Zymo Research, D5455). 7 cycles of amplification was used for library construction ...
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA and RNA were extracted from fresh frozen tumor (n=2) and normal (n=3) samples using the ZR-Duet DNA/RNA MiniPrep extraction kit (ZYMO RESEARCH, Irvine, CA). Quantification was performed with a NanoDrop One™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... from all the experimental groups (n=3/group) was subjected to bisulphite treatment using the EZ DNA methylation™ kit (Zymo Research, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... one set of replicates (total of 3 replicates per treatment) was harvested for DNA and RNA using ZR-Duet DNA/RNA MiniPrep (Plus) kit (Zymo Research Cat # D7003) following manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... We created libraries from bisulfite-converted single stranded DNA using the Pico Methyl-Seq Library Prep Kit (Zymo Research), which were then pooled for sequencing on an Illumina NextSeq 500 instrument using the NextSeq 500/550 V2 High Output reagent kit (1 × 75 cycles ...
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Phage-centric ecological interactions in aquatic ecosystems revealed through ultra-deep metagenomicsbioRxiv - Microbiology 2019Quote: ... were cut in small pieces (≅ 3–5mm) using sterile scissors and processed for DNA extraction using the ZR Soil Microbe DNA MiniPrep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... Then, samples were centrifuged (14000 g, 3 min) and the supernatants were transferred to silica columns to bind DNA (Zymo Research, Irvin, California, USA). After 1 min of incubation ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mL of suspended biomass was collected in triplicate from the mixed tank and PBR effluent and stored in 5 mL polypropylene transport tubes (FisherbrandTM, USA) prefilled with 3 mL DNA/RNA ShieldTM (Zymo Research corp., CA, USA). The samples were briefly vortexed and stored at -20 °C until they were shipped to University at Buffalo (UB ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then treated with Turbo DNAse 2 to 3 times and then purified with the RNA Clean and Concentrator-5 Kit (Zymo Research Cat#: 11-325) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... The whole genome bisulfite sequencing (WGBS) libraries were prepared using Pico Methyl-seq library prep kit (Cat# D5456, Zymo research). Paired-end ...
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bioRxiv - Immunology 2020Quote: ... WGBS libraries were prepared using 100 ng of DNA using the Pico Methyl-Seq™ Library Prep Kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA libraries were prepared genomic DNA (10 ng) using a Pico Methyl-Seq Library Prep Kit (ZYMO, catalog # D5456). MQ beads (Epigentik ...
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bioRxiv - Microbiology 2020Quote: ... and 7) ZymoBIOMICS™ DNA Microprep kit (Zymo Research, California, USA) with modifications ...
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bioRxiv - Systems Biology 2022Quote: ... the second sample was stored at RT for 3 – 5 days and the third sample was collected in a Zymo research tube (Zymo Research Inc., Irvine, CA, USA) containing 9ml DNA/RNA shield and stored at RT for 3 – 5 days before storage at −80 °C ...
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bioRxiv - Immunology 2020Quote: ... the DNA was subjected to bisulfite conversion and PCR-based sequencing adapter tagging as described for WGBS above (Pico Methyl-Seq Library Prep Kit, Zymo Research). The library was quantified and sequenced on an Illumina Nextseq 550 ...
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bioRxiv - Genetics 2020Quote: ... We then prepared whole-genome bisulphite sequencing libraries from each DNA sample using the Pico Methyl-Seq Library Prep Kit (Zymo Research), according to the manufacturer’s protocol (see Supplementary Table 1 for detailed sample metadata and sequence accession codes) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... age-matched (7-10 days post eclosion adults) using Zymo Quick-DNA extraction kit (Zymo D3012). The 16S V1-V2 region was amplified ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from 7-day old gemmae using Direct-Zol RNA MINIprep kit (Zymo Research) following manufactures instructions ...
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bioRxiv - Genomics 2020Quote: ... 7) We verified the presence of high molecular weight DNA products in the samples before purifying nucleic acids by Zymo DNA Clean & Concentrator-5 (ZYMO Research).
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bioRxiv - Microbiology 2021Quote: Infected Huh-7 cells (with or without medium) or mouse lung tissues were lysed in DNA/RNA shield reagent (Zymo Research) and total RNA was extracted by using RNeasy kit (Qiagen ...
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bioRxiv - Genetics 2019Quote: Four 7 micron sections of the left ventricle were used to isolate total RNA using the Quick-RNATM FFPE kit (Zymo Research). 1 µg of total RNA was used to generate cDNAs using the SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from human iPSC-FBs and mouse Raw 264-7 cells using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 ml Trizol (R2050-1, Zymo Research) as well as 200 µl chloroform were added to the interface and phase separation and removal were repeated ...