Labshake search
Citations for Zymo Research :
51 - 100 of 6710 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... The Carbon-Clarke equation was used to calculate how many colonies were needed to ensure a 99% confidence that all guides in the library was represented after each transformation (∼37,000 colonies).20 Colonies were scraped and midi-prepped (Zyppy Plasmid Midiprep Kit, Zymo Research, Irvine, CA) in preparation to be transformed into strain W.
-
bioRxiv - Molecular Biology 2022Quote: ... The dsDNA blocks were excised from the gel and purified to 20 μl mQ using the Zymoclean™ Gel DNA Recovery Kit (Zymo, D4002). 5 μl of the dsDNA was used to quantify the DNA concentration with the Qubit assay (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR reactions containing the dsDNA block were cleaned and concentrated to 20 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 4 μl Gel Loading Dye ...
-
bioRxiv - Microbiology 2021Quote: ... were thoroughly mixed 1:1 with 2X DNA/RNA Shield™ and incubated at RT for 20 min for inactivation and vRNA was extracted using the Quick-RNA™ Viral Kit (Zymo Research) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 20 mg of each tissue using the ZYMO Quick-DNA™ Miniprep Plus Kit (Zymo Research, Irvine, CA). Resulting DNA was amplified neat and diluted 1:2 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... methylated indexed adapters were ligated and 20 µL of cleaned DNA fragments were subjected to sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research, CA). Pre-amplification of the bisulfite-converted library was carried out for an equimolar pool of eight samples and was then subjected to exome capture at 47°C for 45 hours ...
-
bioRxiv - Genomics 2023Quote: ... 20 µL of DNA binding buffer (Zymo) was added to each well and incubated at room temperature for 20 minutes ...
-
bioRxiv - Genetics 2021Quote: ... All samples were randomized into 96-well plates for RNA extraction with the Direct-zol-96 RNA Kit (Zymo Research, Irvine, CA). Libraries constructed with the Lexogen QuantSeq 3′ mRNA-Seq Library Kit FWD (Lexogen ...
-
bioRxiv - Neuroscience 2022Quote: ... total cellular RNA using ∼1×106 cells from one well of a six-well plate was isolated using TRIzol reagent followed by Direct-zol RNA MiniPrep Plus kit purification (Zymo Research # R2070). RNA was quantified using Qubit RNA HS Assay kit and cDNA synthesised from ∼2µg of RNA using SuperScript IV VILO Master Mix kit following gDNA removal with ezDNase ...
-
bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA from individual eGFP+ cell lines was extracted from 96 well-plates using Quick-DNA Universal 96 Kit (Zymo Research, D4071) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Cell pellets collected from 48-well plates were lysed and total RNAs were extracted using the Direct-zolTM RNA Miniprep kit (Zymo Research R2053) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from the mixture of colonies from each plate using a Quick-DNA HMW MagBead Kit (Zymo Research, USA) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... The pellet was frozen at -20°C until DNA extraction using a Zymo fecal/soil DNA extraction kit according to enclosed directions (Zymo Research, Irvine, CA). After sonication ...
-
bioRxiv - Developmental Biology 2020Quote: RNA was isolated directly from the TRIzol muscle samples using a 96 well plate extraction kit (Direct-zol™-96 RNA, Zymo Research, #R2054): after thawing to room temperature in 1,5 ml Eppendorf tubes ...
-
bioRxiv - Genomics 2021Quote: ... DNA samples were plated randomly across 96-well plates and provided to the Emory University Integrated Genomics Core for bisulfite modification using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA), and subsequent assessment of genome-wide DNAm using the Illumina MethylationEPIC Beadarray (Illumina ...
-
bioRxiv - Genomics 2023Quote: ... the supernatant was transferred to a deep 96-well plate and mixed with 400ul of genomic lysis buffer (Zymo, #D3004-1). The mix was stored at -20C to await further processing ...
-
bioRxiv - Cancer Biology 2019Quote: ... 600 ng of purified DNA were randomly distributed on a 96-well plate and processed using the EZ-96 DNA Methylation kit (Zymo Research Corp., CA, USA) following the manufacturer’s recommendations for Infinium assays ...
-
bioRxiv - Microbiology 2020Quote: ... The extracted DNA was then purified in the well-plate format using the ZR-96 Clean and Concentrator kit (Zymo research, Irvine, CA US), following manufacturer’s instructions and eluting in 6 µl of the supplied elution buffer ...
-
bioRxiv - Genomics 2020Quote: ... DNA were extracted from clonally expanded cells (from one well of the culture plate) and from Jurkat cells using Quick-DNA™ Miniprep Plus Kits (Zymo research, catalog no. D4068) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 20 µl of each module (PCR product + Zymo Shield) (6 in total ...
-
bioRxiv - Plant Biology 2022Quote: ... A final concentration of 0.1 ng/µL was employed for both the Femto Fungal DNA Quantification Kit and Femto Bacterial DNA Quantification Kit (Zymo Research) according to the manufacturer protocol ...
-
bioRxiv - Genomics 2020Quote: ... purified using 1 column of Zymoclean Gel DNA Recovery Kit (Zymo Research D4008) and eluted in 20μl ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... DNA was extracted from 1 mL of culture using kit D7005 from Zymo Research ...
-
bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from mixtures of KU-1 or BL3.1 and BTL26 for a total of 1 × 106 cells using Quick-DNA Miniprep Kits (Zymo Research). The DNA concentrations of the samples were measured by UV absorbance at 260 nm using a NanoDrop 8000 Spectrophotometer (Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2019Quote: ... or by sodium bisulfite using two commercially available kits: BS (1)EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA Research) and BS (2 ...
-
bioRxiv - Genetics 2020Quote: ... DNA was digested in columns by adding a mixture of 5 μl RNA Lysis Buffer 1 from the kit andl DNase I and 75 μl RNA Lysis Buffer 1 from the kit andl DNA Digestion Buffer from DNase I Set kit (Zymo Research E1010) followed by a 15-minute incubation at room temperature ...
-
bioRxiv - Genomics 2023Quote: ... and samples were eluted in 20 μl Elution Buffer (Zymo).
-
bioRxiv - Molecular Biology 2021Quote: ... transferred to a Direct-zol spin plate (Zymo Research), and RNA was extracted according to the Direct-zol protocol (Zymo Research).
-
bioRxiv - Microbiology 2021Quote: ... transferred to a Direct-zol spin plate (Zymo Research), and RNA was extracted according to the Direct-zol protocol (Zymo Research).
-
bioRxiv - Genomics 2022Quote: ... transferred to a Direct-zol spin plate (Zymo Research), and RNA was extracted according to the Direct-zol protocol (Zymo Research).
-
bioRxiv - Microbiology 2024Quote: ... The upper aqueous phase was transferred to an RNase-free tube and mixed in a 1:1 ratio with 100% EtOH and processed via the clean and concentrator kit (Zymo Research). RNA was eluted in 15 µL RNase-free ddH2O ...
-
bioRxiv - Microbiology 2024Quote: ... 250 µl aqueous phase was mixed 1:1 with 75% ethanol and transferred into the Zymo-Spin IC Column from the RNA Clean & Concentrator Kit (Zymo Research). RNA clean-up and collection was done following the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2024Quote: ... The RNA was mixed with ethanol absolute at a 1:1 v/v ratio and submitted to a spin column-based purification protocol (Direct-zol RNA Miniprep kit, Zymo Research), including a DNAse treatment step ...
-
bioRxiv - Bioengineering 2020Quote: ... Cells were first grown in YPD overnight and then streaked on SC agar plates containing 1 mg/mL 5-FOA (Zymo Research, Orange, CA, USA). A single colony from an SC/5-FOA agar plate was streaked again on a new SC/5-FOA agar plate ...
-
bioRxiv - Molecular Biology 2019Quote: ... Genomic DNA was isolated from 1 ml quiescent fractions (YeaStar kit, Zymo Research, Protocol I), and the DNA eluted with 60 μl of TE buffer (10 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... Genomic DNA (1 μg) was converted using the ZYMO EZ DNA Methylation-Gold kit (ZYMO) and one twentieth of the elution products were used as templates for PCR amplification ...
-
bioRxiv - Molecular Biology 2019Quote: Plasmids were extracted from yeast with Yeast Plasmid Miniprep 1 kit (Zymo Research, D-2001). Library fragments were amplified in 17 PCR cycles using primers specific to DNA sequences that flank Aβ-DHFR in p416 ...
-
bioRxiv - Genomics 2021Quote: RNA extractions from 1×10E6 cells were performed with Quick RNA Miniprep Kit (Zymo Research). 140 ng-500 ng RNA were retro-transcribed with Reverse Aid reverse transcriptase (Thermo Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... containing 1% BME and total RNA purified using the Quick-RNA Microprep kit (Zymo Research). All resulting RNA was used as an input for complementary DNA synthesis using the SMART-Seq v4 kit (Takara Bio ...
-
bioRxiv - Molecular Biology 2020Quote: ... 100 % ethanol was added to the aqueous phase (1:1, v/v) and transferred to RNA Clean and Concentrator (RCC) ™ −5 kit (Zymo Research; R1013) and centrifuged at 16,000 x g for 30 s ...
-
bioRxiv - Microbiology 2019Quote: ... RNA was extracted from 1−3 × 106 cells using the Quick-RNA Miniprep Kit (Zymo Research) as per manufacturer’s instructions ...
-
bioRxiv - Genetics 2020Quote: 1 μg of total RNA was bisulfite-modified with the EZ RNA Methylation Kit (Zymo Research). Briefly ...
-
bioRxiv - Synthetic Biology 2022Quote: ... plasmids were extracted from 1 ml of culture using the ZymoPURE Plasmid Miniprep Kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: Total RNA was isolated from 1 million cells using Quick RNA Miniprep Kit (Zymo Research #R1055) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: RNA extractions were performed using ∼1 million cells using Quick-RNA MicroPrep Kit (Zymo Research, Irvine, CA). RT-qPCR for HOXA9 used 100 ng of RNA for cDNA synthesis (Applied Biosystems ...
-
bioRxiv - Plant Biology 2019Quote: ... 1 μg of genomic DNA was treated with sodium bisulfite using an EZ DNA Methylation Kit (Zymo). The treated DNA was used as the templates to amplify the OsFIE1 and OsFIE2 segments (150–300 bp ...
-
bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from 1×106 primary melanocytes (MC) using an RNA isolation kit (Zymo Research) as per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Bisulfite conversion of 1 µg genomic DNA was performed using EZ DNA Methylation Kit (D5001, Zymo Research). The bisulfite-converted DNA samples were subject to genome-wide methylation analysis using the Illumina Infinium MethylationEPIC BeadChips ...
-
bioRxiv - Genetics 2020Quote: ... Generated amplicons were purified from 1% agarose gels using the Zymoclean Gel DNA Recovery Kit (Zymo Research) before library preparation.
-
bioRxiv - Genomics 2023Quote: ... The product was extracted from a 1% agarose-TBE gel (Zymo Gel Extraction kit, 10 µL elution), then assembled with the 5′ UTR gBlock in a Gibson reaction ...