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Citations for Zymo Research :
851 - 900 of 1026 citations for 7 TRIFLUOROMETHYL 2 3 4 5 TETRAHYDRO 1H BENZO B AZEPINE HYDROCHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Total RNA from approximately 2 × 106 cells were isolated using the Direct-Zol RNA isolation kit (Zymo Research). RNAseq libraries were prepared as previously described 70 using KAPA Standed RNA-seq library preparation kit (KAPA Biosystems) ...
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bioRxiv - Systems Biology 2020Quote: ... 2 μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
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bioRxiv - Plant Biology 2021Quote: ... 500 pg - 2 µg DNA was treated with sodium bisulfite using the EZ DNA Methylation kit (Zymo Research). Amplicons for sequencing were generated by PCR using degenerate primers (see Supplemental Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 2 hours at 45°C and purified using the ChIP DNA Clean and Concentrator Kit (Zymo Research).
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bioRxiv - Genomics 2021Quote: ... RNA was isolated from 2 day-differentiated TXΔXicB6 cells using the Direct-Zol RNA Miniprep Kit (Zymo Research). To remove any remaining gDNA ...
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bioRxiv - Immunology 2021Quote: Total RNA isolations from SARS-CoV-2 were carried using DIRECT-ZOL RNA Miniprep Kits (Zymo Research, USA), and concentrations were determined using QUBIT fluorometer with the QUBIT RNA HS Assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from 2×106 NCI-H295R cells was isolated with the Quick-DNA Miniprep Kit (Zymo research). Bisulfite treatment was performed using the EZ DNA Methylathion™ Kit (Zymo Research) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... for 2 min at 30Hz and extracted RNA using the Direct-Zol RNA MiniPrep kit (Zymo Research, USA) with on-column DNA digest according to the manufacturer protocol ...
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bioRxiv - Genomics 2023Quote: ... 2 x 500 ng of DNA was bisulfite modified with the EZ DNA Methylation-Lightning kit (\#D5030, Zymo) and standardized to a concentration of 25 ng/uL BC-DNA ...
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bioRxiv - Systems Biology 2023Quote: ... We then sequentially added and centrifuged through the column 400 µL DNA/RNA prep buffer (Zymo, #D7010-2), 600 µL DNA/RNA wash buffer (Zymo ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were then harvested by centrifugation at 3500g for 4 min and prepared for transformation using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). For plasmid transformations ...
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bioRxiv - Microbiology 2023Quote: ... control samples (n = 4, one for each extraction batch) using commercially available kits optimized for fecal-based microbial communities (Zymo fecal/soil kit), and some aliquots archived ...
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bioRxiv - Microbiology 2019Quote: ... The enriched circular DNA samples were then purified by DNA Clean & ConcentratorTM-5 kit according to manufacturer’s instructions (Zymo Research, USA). Elution was done with 10 µl of 10 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2022Quote: ... TurboDNase treatment as described above usually removes most genomic DNA and small fragments of DNA that could prime as random primers (detectable by ‘no RT primer’ control) are cleared away efficiently by the RNA Clean & Concentrator-5 kit (Zymo Research). Hence ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from 150 µL of the fractionated samples using an RNA clean and concentrator-5 kit (Zymo Research, R1016) using the same procedure to extract RNA from unfractionated cell lysate (described above) ...
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bioRxiv - Molecular Biology 2021Quote: ... purified PCR product was digested with EcoRI-HF and BamHI-HF at 37°C for 1.5 hours and purified again using a Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). For the cloning backbone ...
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bioRxiv - Developmental Biology 2019Quote: ... Sorted cells were collected in PBS, centrifuged (2000 rpm, 5 min) and RNA extracted using the Quick RNA Mini Prep (Zymo Research) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... The assembled DNA was then cleaned and concentrated using Zymo Clean and Concentrator-5 kit (SKU D4013 Zymo Research, Irvine, CA), quantified by Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2019Quote: ... Total RNA was isolated from cell pellets in 5 batches (each batch contained one of each of the 5 strains) using the ZR Fungal/Bacterial RNA mini-prep kit including the DNase I on-column digestion step (Zymo Research). Each cell pellet was resuspended in lysis buffer and transferred to screw-capped tubes containing glass beads and the cells were broken open using a mini-beadbeater (Biospec Products ...
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bioRxiv - Genomics 2019Quote: ... The poly(A)-tailed Mb3Cas12a mRNAs were purified using the RNA Clean & Concentrator™-5 (Cat. R1016, Zymo Research, Irvine, CA) and eluted in a Tris-EDTA solution (Cat.11-01-02-02 ...
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bioRxiv - Developmental Biology 2019Quote: The cDNA of all single cells was collected together and further concentrated with the DNA Clean and Concentrator−5 kit (Zymo Research). The Exonuclease I (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... 5 heads of each genotype were pooled and total RNA extracted using the Direct-zol RNA Microprep kit (Zymo Research, R2060) and the quality determined using a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Genetics 2020Quote: ... all PCR products were cleaned using a Zymo Research ZR-96 DNA Clean & Concentrator™-5 (Zymo Research, Irvine, California, USA). Samples were eluted in 50µl of DNA free water.
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bioRxiv - Bioengineering 2020Quote: ... at 37°C for 24 hours to ensure complete removal of any 5’-triphosphate moieties then purified again using a RNA spin column (Zymo Research). Lastly ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared supernatant was then transferred to a fresh reaction tube and applied to spin-columns (RNA Clean & Concentrator-5 columns, Zymo-Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The reaction was then treated with DNase I to remove template DNA and RNA product was purified using an RNA Clean and Concentrator-5 (Zymo #R1016). Purified RNA was used as the template for reverse transcription using ProtoScript II according to manufacturer protocol ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the DNA template via in vitro transcription (IVT ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA precipitants were treated with 100 μl of 0.04 U Turbo DNase I at 25°C for 30 min to remove DNA contamination and cleaned using an RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Research, USA). The RNA was eluted in 16 μl RNase-free water for subsequent experiments ...
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bioRxiv - Cell Biology 2021Quote: ... the RNA was cleaned to remove enzyme and buffer components using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1014) before the next treatment ...
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bioRxiv - Microbiology 2021Quote: ... rRNA depleted RNA was purified from the reaction mixture with the Zymo RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA) according to manufacturer instructions and eluted in 12 µl nuclease-free water ...
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bioRxiv - Synthetic Biology 2021Quote: ... After the second treatment with DNase I the samples were column purified and concentrated using RNA Clean & Concentrator-5 kit (Zymo Research). Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... The digested fragments were purified using the DNA Clean & Concentrator-5 Kit and the Zymoclean Gel DNA Recovery Kit (Zymo Research) respectively ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Mixture was incubated at 37 ℃ for 30 min followed by purification with a Zymo DNA Clean and Concentrator-5 Kit (Zymo, DP4033). Transposed DNA was eluted in 24 μl elution buffer and amplified by PCR reaction (10 μl 5 X TAB ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-5 and LPI-15 were collected from three greenhouse-grown WT plants (∼5 ft in height) for RNA extraction using Direct-zol RNA MiniPrep kit (Zymo Research) with Plant RNA Purification Reagent (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... 1.5 µg of genomic DNA in 30 ml ddH2O was digested to free nucleosides using 5 U of DNA Degradase Plus (Zymo Research) in 25 μl reactions incubated for 2 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Products were checked on an agarose gel and cleaned-up through a PCR-clean up kit (DNA Clean and Concentrator-5, Zymo, D4013), followed by a secondary PCR to add illumina indexes (SFig ...
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bioRxiv - Molecular Biology 2020Quote: 15 µl of UltraPure water was added to each assembly reaction on ice and 2.5 µl of these diluted reactions were then transformed into 50 µl chemically competent DH5α cells (prepared in house, Zymo T3001) on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... Gel band within 100-250bp was cut and purified using Zymoclean Gel DNA recovery kit/ DNA Clean & Concentrator-5 (Zymo Research). If needed ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by a 5-day incubation period before total RNA was collected by Direct-zol RNA MiniPrep Plus kit (Zymo Research). 69 RNASeq profiles ...
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bioRxiv - Genomics 2022Quote: ... The samples were treated with DNase I and cleaned using ethanol precipitation and subsequent RNA Clean & Concentrator-5 spin-column purification (Zymo Research). Successful removal of genomic DNA was confirmed by PCR using a host-specific primer pair (Supplementary Figure S1) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).
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bioRxiv - Microbiology 2022Quote: ... were incubated in a total volume of 20 µL for 30 min at 37°C and subsequently column-purified using RNA Clean and concentrator 5 (Zymo Research).
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bioRxiv - Cancer Biology 2023Quote: ... Initial genomic library distributions were assessed on a 1.5% agarose gel and purified using a ZR-96 Genomic DNA Clean & Concentrator-5 Kit (Zymo Research, D4067). Library concentrations were quantified using the Quant-iT™ dsDNA Broad Range Assay Kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Viral gene sequences in pPolI plasmids for all PR8 segments were amplified by PCR using corresponding primer pairs and were purified using ZYMO Research DNA cleaner and Concentrator-5 (ZYMO, D4014). Purified PCR products were in vitro transcribed using the T7 RiboMAX Express Large Scale RNA Production System (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA from each fraction was first precipitated in EtOH/C2H3NaO2 and then purified through RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Reaserach, R1016). RNA distribution profiles were analyzed by qRT-PCR.
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bioRxiv - Systems Biology 2023Quote: ... We ran this concentrated library on a 2% agarose 1X TAE gel with an EtBr stain at 90 V for 85 minutes and extracted the resulting 636 bp band using a DNA Clean & Concentrator-5 Kit (Zymo, #D4014) with a 20 μL MB H2O elution ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at 37°C for 1 hour and then purified with Zymo DNA Clean & Concentrator-5 (Zymo Research) following the PCR product protocol and eluting DNA into 10.5 μL of water ...