Labshake search
Citations for Zymo Research :
851 - 900 of 1055 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from rosette tissue harvested at ZT 23 on day 25 post imbibition (n = 4 biological replicates) using the Direct-zol-96 MagBead RNA kit (Zymo Research). RNA was checked for degradation using a Tapestation 4150 (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA from 105 days old CorticOs (N = 4; n = 6-8 per genotype) was extracted using the Direct-zol RNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2023Quote: ... The lysates were centrifuged at 5000 x g for 10 min at 4 °C and supernatant added to gDNA extraction columns (Zymo Research). The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research ...
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bioRxiv - Developmental Biology 2024Quote: 20 zebrafish embryos per biological replicate were collected at 4 or 6 hpf and snap-frozen in 300 µl of DNA/RNA Shield (#R1100-50, Zymo Research). Total RNA then was extracted using Direct-zol RNA Miniprep Kit (#R2050 ...
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bioRxiv - Molecular Biology 2024Quote: ... The isolated yeast populations obtained after round 4 were subjected to colony screening and plasmid recovery using a Zymoprep yeast plasmid kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was isolated from the fresh feces collected from UT1- or PBS-gavaged mice (female, n = 4/group) using a ZymoBIOMICS DNA Miniprep Kit (Zymo Research). Note ...
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bioRxiv - Biophysics 2021Quote: ... The DNA was then purified by gel extraction and a DNA Clean & Concentrator −5 kit (Zymo Research, cat#D4014). DNA quality was determined by 260nm/230nm and 260nm/280nm ratios on a DS-11+ spectrophotometer (DeNovix ...
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bioRxiv - Microbiology 2021Quote: ... The pooled sample library was concentrated using DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). The pooled library concentration was determined using the Quant-iT™ High-Sensitivity DNA Assay Kit (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Zip code amplicons were purified using the DNA Clean and Concentrator-5 kit (Zymo Research, CA. Cat. No. D4013) and eluted in 15 μL of Milli-Q ® H2O ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA from each of the tubes were purified using the Clean-and-Concentrator-5 columns (Zymo, #D4004, Irvine, CA) using 5X binding buffer to increase yield ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were stopped by cleaning up the samples following the ZYMO RNA Clean & Concentrator-5 kit (ZYMO, R1013) protocol ...
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bioRxiv - Microbiology 2020Quote: ... pooled in equimolar concentrations and concentrated using the DNA Clean & Concentrator™-5 Kit (Zymo Research, Irvine, CA, USA). Sequencing of the amplicon library was performed on the Illumina MiSeq System with MiSeq reagent kit v2 (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: ... and RNA >200 nt was purified using an RNA Clean & Concentrator-5 Kit per the manufacturer’s instructions (Zymo Research). Ribosomal RNA was depleted using a MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... RNA was then purified and concentrated on a RNA Clean Concentrator™-5 column (Zymo Research, Irvine, CA, USA).
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bioRxiv - Biophysics 2021Quote: ... The samples were then purified by gel extraction and a DNA Clean & Concentrator −5 kit (Zymo Research, cat#D4014). PCR product solution were then phosphorylated with a second round of T4 PNK ...
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bioRxiv - Molecular Biology 2023Quote: ... A portion of the RNA was purified a second time with the RNA Clean & Concentrator-5 kit (Zymo Research), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Free RNAs were extracted using phenol/chloroform and purified with the RNA Clean & Concentrator-5 kit (Zymo Research, R1015).
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bioRxiv - Biochemistry 2022Quote: ... Eluted RNA (total 200 µL) was purified and concentrated using RNA Clean and Concentrator-5 kit (R1015, Zymo Research). 10 ng of purified fragmented total RNA (before adding the antibody/ IgG ...
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bioRxiv - Genomics 2023Quote: ... the resulting poly-A+ RNA fraction (corresponding to 0.7-2% of the starting amount) was then purified again on a Clean & Concentrator-5 Column Kit (Zymo) and eluted in 10µL of molecular-grade water.
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bioRxiv - Genomics 2023Quote: ... according to manufacturer’s instructions followed by a clean up using the RNA Clean & Concentrator-5 kit (Zymo Research, R1013). Concentrations were determined using the Qubit 2.0 Fluorometer ...
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bioRxiv - Neuroscience 2024Quote: ... the RNA samples were purified and concentrated using an RNA Clean & Concentrator-5 Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The total RNA and RF samples were purified using a modified RNA Clean & Concentrator-5 kit method (Zymo Research). The volume of each sample was increased to 100 µl ...
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bioRxiv - Genomics 2023Quote: ... cytosines in 87bp DNA fragment were modified with hydroxy-methylation by PCR amplification with 5-hydroxymethyl-dCTP supplement (ZYMO). The hydroxy-methylated DNA fragment was combined with equal amounts of CpG methylated pUC19 DNA (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Added water to volume of 50 μL and cleaned up the RNA (Zymo RNA clean and concentrator -5 # R1013) and eluted in 15 μL water.
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were placed on ice and then purified using Zymo DNA clean and concentrator kit -5 (Zymo #D4004) following cDNA protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cfRNAs were then purified and concentrated into 6 µl using an RNA Clean and Concentrator-5 kit (Zymo, R1016). Plasma extracellular vesicles (EVs ...
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bioRxiv - Genomics 2023Quote: ... cleaned up either with phenol-chloroform and precipitated with ethanol or with RNA Clean & Concentrator -5 kit (Zymo Research). Two biological replicates were collected for each genotype ...
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bioRxiv - Microbiology 2023Quote: ... The RNA samples were cleaned and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... The reaction was purified and size selected to >200 nts using Zymo RNA Clean and Concentrator-5 (Zymo #R1013) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... DNase I treated RNA samples were purified and further concentrated using RNA clean and concentrator-5 (Zymo Research, R1014) according to the manufacture’s protocols ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was size-selected to ≥200 nts using the Zymo RNA Clean and Concentrator-5 kit (Zymo #R1013) according to manufacturer’s protocol and eluted in 6 µl of nuclease-free water.
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bioRxiv - Molecular Biology 2024Quote: ... The RNA was then purified and concentrated using RNA Clean and Concentrator-5 spin columns (Zymo Research; Catalog #R1015).
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNA (3µg) was purified with RNA Clean and Concentrator-5 kit (Zymo Research, Irvine, CA, USA, Ref. ZR1016), keeping only small RNAs fragments (17 - 200 nt ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 5-week-old plant leaves using Direct-zol RNA Miniprep kit (Zymo research, R2050). Library preparation was performed using NEXTFLEX® Small RNA-Seq Kit v3 (Cosmobio ...
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bioRxiv - Biochemistry 2024Quote: ... Fragmented mRNA was then size selected by differential silica binding with RNA Clean & Concentrator-5 spin columns (Zymo Research) using 33% ethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... The final purification of amplified DNA was performed with the DNA clean and Concentrator-5 kit (Zymo Research, D4013) and quantified using NanoDrop one (260nm) ...
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bioRxiv - Developmental Biology 2024Quote: ... for 5 minutes and then RNA was purified using Direct-zol RNA Miniprep kit according to the manufacturer’s instructions (Zymo). RNA was reverse transcribed using UltraScript cDNA synthesis kit (PCR Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and 3 swabs (2 for metagenomic DNA isolation, 1 for storage) moistened with DNA/RNA shield (Zymo Research, Cat. No. ZYR.R1100-250). Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e ...
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bioRxiv - Plant Biology 2022Quote: ... Then, samples were centrifuged (14000 g, 3 min) and the supernatants were transferred to silica columns to bind DNA (Zymo Research, Irvin, California, USA). After 1 min of incubation ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Microbiology 2024Quote: ... The column was then washed with 200 μl of RNA wash buffer and subjected to desulfonation by adding 200 μl of RNA desulphonation buffer (Zymo Research #R5001-3-40) to the column ...
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bioRxiv - Molecular Biology 2024Quote: ... Coprecipitated proteins were released from the beads by heating/denaturation (CBD-, s-AP) in 1 × loading buffer or eluting with Strep-Elution buffer (Strep-AP, Zymo Research, P2004-3-30) or lysis buffer containing 3xFlag peptide (Flag IP ...
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bioRxiv - Genomics 2024Quote: ... and three susceptible (n= 3) untreated seedlings were used for genomic high molecular weight DNA isolation using the Quick DNA HMW MagBead Kit (Zymo Research, Irvine, CA, USA). DNA integrity ...
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bioRxiv - Genomics 2020Quote: DNA was extracted using 4-10 unstained slides of FFPE tissue using the Pinpoint Slide DNA Isolation system (Zymo Research, Orange, CA). In brief ...
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bioRxiv - Genetics 2024Quote: ... GMSM-K - 6 non-risk and 7 risk genotype for rs11119348 and 4 each genotype for rs661849) using Quick-DNA/RNA Miniprep kit (Zymo Research #D7001) followed by DNase treatment using manufacturer’s instructions (Thermo Fisher Scientific #AM1907) ...
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bioRxiv - Biochemistry 2024Quote: ... at 37 °C for specific time (20 min, 1 hour, 2 hours, 4 hours, or 16 hours) followed by Oligo Clean & Concentrator Kits (OCC, Zymo Research, D4061) cleanup ...
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bioRxiv - Molecular Biology 2020Quote: ... treated for two hrs using 2 units of DNase I per hour and was cleaned of residual DNase I with the RNA clean and concentrator-5 (Zymo). DNase-treated RNA was then divided into two equal aliquots ...
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bioRxiv - Molecular Biology 2021Quote: ... For RNA analysis 5% of input material and 70% of elution were processed by the addition of 400 µl RNA lysis buffer (Zymo) and RNA extraction using the Zymo Quick-RNA™ MicroPrep RNA extraction kit (Zymo ...
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bioRxiv - Molecular Biology 2020Quote: ... The enriched RNA and input total RNA from 5% of lysate were purified using Direct-zol RNA Purification Kit (Zymo). Target genes in input total RNA and enriched RNA were quantified by qRT-PCR using SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... the transposed DNA fragments were harvested and cleaned up with Zymo DNA Clean and Concentrator-5 kit (D4011, Zymo Research). The libraries were indexed and amplified with Illumina i5/i7 indexing primers (FC-121-1011 ...